Investigation of DMSO-Induced Conformational Transitions in Human Serum Albumin Using Two-Dimensional Raman Optical Activity Spectroscopy

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Microsatellite markers for Bokermannohyla species (Anura, Hylidae) from the Brazilian Cerrado and Atlantic Forest domains

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

O padrão de compressão de video MPEG moving picture experts group

This work focuses on the study of video compression standard MPEG. To this end, a study was undertaken starting from the basics of digital video, addressing the components necessary for the understanding of the tools used by the video coding standard MPEG. The Motion Picture Experts Group (MPEG) was formed in the late '80s by a group of experts in order to create international standards for encoding and decoding audio and video. This paper will discuss the techniques present in the video compression standard MPEG, as well as its evolution. Will be described in the MPEG-1, MPEG-2, MPEG-4 and H.264 (MPEG-4 Part 10), however, the last two will be presented with more emphasis, because the standards are present in most modern video technologies, as in HDTV broadcasts

Metabolomics method to comprehensively analyze amino acids in different domains

Amino acids play essential roles in both metabolism and the proteome. Many studies have profiled free amino acids (FAAs) or proteins; however, few have connected the measurement of FAA with individual amino acids in the proteome. In this study, we developed a metabolomics method to comprehensively analyze amino acids in different domains, using two examples of different sample types and disease models. We first examined the responses of FAAs and insoluble-proteome amino acids (IPAAs) to the Myc oncogene in Tet21N human neuroblastoma cells. The metabolic and proteomic amino acid profiles were quite different, even under the same Myc condition, and their combination provided a better understanding of the biological status. In addition, amino acids were measured in 3 domains (FAAs, free and soluble-proteome amino acids (FSPAAs), and IPAAs) to study changes in serum amino acid profiles related to colon cancer. A penalized logistic regression model based on the amino acids from the three domains had better sensitivity and specificity than that from each individual domain. To the best of our knowledge, this is the first study to perform a combined analysis of amino acids in different domains, and indicates the useful biological information available from a metabolomics analysis of the protein pellet. This study lays the foundation for further quantitative tracking of the distribution of amino acids in different domains, with opportunities for better diagnosis and mechanistic studies of various diseases.

Durable bonds at the adhesive/dentin interface: an impossible mission or simply a moving target?

Composite restorations have higher failure rates, more recurrent caries and increased frequency of replacement as compared to dental amalgam. Penetration of bacterial enzymes, oral fluids, and bacteria into the crevices between the tooth and composite undermines the restoration and leads to recurrent decay and failure. The gingival margin of composite restora tions is particularly vulnerable to decay and at this margin, the adhesive and its seal to dentin provides the primary barrier between the prepared tooth and the environment. The intent of this article is to examine physico-chemical factors that affect the integrity and durability of the adhesive/dentin interfacial bond; and to explore how these factors act synergistically with mechanical forces to undermine the composite restoration. The article will examine the various avenues that have been pursued to address these problems and it will explore how alterations in material chemistry could address the detrimental impact of physico-chemical stresses on the bond formed at the adhesive/dentin interface.

Influência do tempo sedentário na qualidade de vida e na percepção dos estados de ânimo em idosos fisicamente ativos

Introduction: Sedentary behavior is characterized by individuals who designed much of the day sitting or lying down activities, even if such conduct regular physical activity. Over the years people tend to be less active, worsening the quality of life. For healthy aging is necessary for the individual to be constantly active, and moving activities improve mood and quality of life, though little is known of the influence of sedentary behavior in these variables. Aim: To investigate the association between sedentary time, quality of life and mood states of elderly women engaged in physical activity. Methodology: The study included 68 elderly women who belong to the Physical Activity Program for the Elderly (PROFIT), for at least 6 months. These answered the Sedentary Behaviour Questionnaire, the Pentacle Welfare and IPAQ Short Version. The Pep States List Reduced and Illustrated (LEA-RI) was applied before and after a workout and before and after three times of sedentary behaviors. To evaluate the difference of sedentary behavior time average for each domain of quality of life was conducted the analysis of the 95% confidence interval for the STATA program version 12.0 and to the moods list data a non-parametric test - test binomial using SPSS version 17.0 program and has adopted a p≤0,05. Results: A moderate intensity physical activity was the most practiced (939.5 ± 650.2 min / week) and the activity most frequently performed in sedentary time was watching television (187.6 ± 96,4min / day). In general, the quality of life for the elderly is considered good (98.39%) and the disease is most prevalent hypertension (29.47%). After a physical activity older felt less useless, less shy and less fear and after a sedentary behavior, felt less agitated and less useless. In relation between the domains of QOL and sedentary behavior time, there were no statistical differences. Conclusion: Elderly active even after a sedentary behavior...