Efficiency of aquatic macrophytes to treat Nile tilapia pond effluents

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Valor nutritivo de silagens de milho (Zea mays L.) produzidas com inoculantes enzimo-bacterianos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Impact of different grazing systems for bovine cattle on the soil microbiological and chemical characteristics

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Bromeliad-living spiders improve host plant nutrition and growth

Although bromeliads are believed to obtain nutrients from debris deposited by animals in their rosettes, there is little evidence to support this assumption. Using stable isotope methods, we found that the Neotropical jumping spider Psecas chapoda (Salticidae), which lives strictly associated with the terrestrial bromeliad Bromelia balansae, contributed 18% of the total nitrogen of its host plant in a greenhouse experiment. In a one-year field experiment, plants with spiders produced leaves 15% longer than plants from which the spiders were excluded. This is the first study to show nutrient provisioning in a spider-plant system. Because several animal species live strictly associated with bromeliad rosettes, this type of facultative mutualism involving the Bromeliaceac may be more common than previously thought.

Influence of water level variation on biomass and chemical composition of the aquatic macrophyte Eichhornia azurea (Kunth) in an oxbow lake of the Rio Mogi-Guacu (São Paulo, Brazil)

The influence of water level variation (flood pulse) on the biomass and chemical composition of the aquatic macrophyte Eichhornia azurea, was investigated in a tropical oxbow lake of the Rio Mogi-Guacu, State of São Paulo, Brazil. The flood pulse causes an increase in total nitrogen content from 0.67 to 1.35 mg/L and total phosphorus content from 10.5 to 101.0 mu g/L of the water. This fertilization, associated with other factors, determines a typical seasonal variation in the biomass and chemical composition of the macrophyte.

Cultivation of Rubrivivax gelatinosus in fish industry effluent for depollution and biomass production

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Determinação bromatológica de Pleurotus ostreatus cultivado em resíduos de diferentes cultivares de bananeira

The objective of this work was to evaluate the nutritional composition (raw protein, ether extract, raw fiber, FDA, FDN and ash) of the POS 09/100 strain of Pleurotus ostreatus grown in three combinations of residues (pseudo-stem, leave and pseudo-stem + leaf) and four banana tree cultivars (Thap maeo, Prata ana, Pelipita and Caipira). The basidiomata harvested were submitted to dehydration in a forced ventilation stove with temperature adjusted to 40 degrees C until reaching constant weight, being then grinded in a knife mill. The method proposed by Kjeldahl (1883) was used to determine total nitrogen. The Weende method (AOAC, 1997) was employed to determine crude fiber, acid-detergent fiber (ADF) and neutral detergent fiber (NDF). According to the results obtained, crude protein, ash, ADF and NDF contents varied with the kind of residue and banana tree cultivar; the substrate prepared with leaves of banana tree (Prata Ana cultivar) yielded the highest crude protein (35.4%) and ash (6.7%) contents in the basidiomata of P. streams; the substrate prepared with pseudo-stein of banana tree (Prata Ana cultivar) provided the highest ADF (31.6%) and NDF (41.9%) contents in the basidiomata of P. ostreatus. Ether extract and crude fiber contents of P. ostreatus didn't vary in function of the treatments, with averages of 2.45 and 11.27%, respectively.

Changes in protein fractions, trypsin inhibitor and proteolytic activity in the cotyledons of germinating chickpea

The chickpea seed germination was carried out in 6 days. During the period it was observed a little variation on total nitrogen contents, however the non protein nitrogen was double. A decrease of 19.1 and 20.6% in relation to total nitrogen was observed to the total globulin and albumin fractions, respectively. The gel filtration chromatography on Sepharose CL-6B and SDS-PAGE demonstrated alterations on the distribution patterns of the albumin and total globulin fractions between the initial and the sixth day of germination suggesting the occurrence of protein degradation in the germination process.The assay for acid protease only appeared in the albumin fraction with casein and chickpea total globulin as substrates, whereas the former was more degradated than the latter, however the transformations detected in the protein fractions apppear indicated that others enzymes could be acting during the process. The trypsin inhibitor activity had a little drop after six day of germination indicating a possible increase on the digestibility of the proteins.

Distribuição e abundância de larvas de Simulium spp.em córregos do estado de São Paulo nos diferentes níveis de qualidade da água

The aim of this work was to determine the distribution and abundance the species of Simulium: in 5 streams of the north coastal region of the state of São Paulo. Simulium larval and water samples were collected every 2 weeks in summer and winter, 2001. The larval were collected on artificial substrates (triplicate) preserved in alcohol (70%) and after analyzed quantitative and qualitative. From the streams the values of temperature, dissolved oxygen, conductivity, pH and stream discharge were measured. The contents of total nitrogen and dissolved nitrogen, ammoniacal nitrogen, nitrate, nitrite, orthophosphate, dissolved phosphorus and total phosphorus were determined in the laboratory. Water quality has great influence in the distribution and abundance of the species. S. pertinax was more abundant in streams without or with low levels of pollution, S. inaequale in streams more polluted and S. incrustatum in intermediary levels of pollution. S. pertinax was the species more abundant with 57.5% of presence in the samples, the second more abundant was S. inaequale with 42.1% of presence and S. incrustatum was rare with 0.4% of presence. The larval abundance was lower in the polluted environment. © 2012 by Unisinos.

Use of a new milk-clotting protease from Thermomucor indicae-seudaticae N31 as coagulant and changes during ripening of Prato cheese

Prato cheeses were manufactured using coagulant from Thermomucor indicae-seudaticae N31 or a commercial coagulant. Cheeses were characterised using the following analysis: yield; fat; acidity; moisture; ash; salt; pH; total nitrogen; total protein; NS-pH 4.6/NT100; NS-TCA 12%/NT100; casein electrophoresis; and RP-HPLC. The results were statistically analysed and revealed that the proteolytic indices were not significantly different throughout the 60 days of ripening of cheeses made with either coagulant. Even though there were some quantitative differences in the peptide profile of cheeses, the enzyme from T. indicae-seudaticae N31 was used in the production of good quality Prato cheese without having to change the established technological parameters of the process. © 2011 Elsevier Ltd. All rights reserved.

Determinação bromatológica de pleurotus ostreatus cultivado em resíduos de diferentes cultivares de bananeira

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Acúmulo de biomassa em variedades de cana-de-açúcar inoculadas com diferentes estirpes de bactérias diazotróficas

The objective of this study was to evaluate the contribution of inoculation with diazotrophs, applied both individually and in combination, in commercial varieties of sugarcane. The experiment was carried out at the experimental grounds of Embrapa Agrobiologia, in Seropédica, Rio de Janeiro, Brazil. The experimental design was of randomised blocks, with sub-divided plots and six replications. Six varieties of sugarcane with six treatments were used: control, control with nitrogen, inoculation of the individual strains: BR 11512, BR 11724 and BR 11411, in addition to inoculation with a cocktail consisting of five strains of diazotrophs. Differences were observed between varieties and between treatments, and an interaction observed between the treatments and varieties. For all the parameters evaluated, no response was observed in the sugarcane varieties RB855536 and RB92606. In the variety RB918639, the only difference was in the accumulation of green biomass. No difference was observed between the treatments for total nitrogen accumulation. The most responsive variety to inoculation was RB867515, differing in the accumulation of fresh and dry biomass. This study showed that inoculation promotes the accumulation of biomass, the contribution being different for the varieties and strains of bacteria, suggesting an interaction between the factors studied. The variety RB867515 is promising for use in studies of inoculation with diazotrophs.

Efeito da inulina e de probióticos sobre a digestibilidade dos nutrientes e balanço do nitrogênio em leitões

This study aimed to evaluate different inulin and probiotic levels as supplement in diets for piglets on nutrient digestibility and nitrogen balance. Twenty four crossbred barrows (Pietráin × Landrace × Large White), with initial average weight of 18.00 ± 0.38 kg, were individually housed in metabolic cages. The experimental design was a completely randomized block, in a 2 × 3 factorial scheme (probiotic levels: 0.30 and 0.60 %; inulin levels: 0.00, 0.25 and 0.50 %), with four replications. The probiotic used was a mix of Lactobacillus acidophillus, Streptococcus faecium and Bifidobacterium bifidum. The inulin was the prebiotic used in this study, characterized as an indigestible carbohydrate formed by fructooligosaccharides. Inulin levels provided a quadratic effect (p<0.05) on the digestibility coefficients of dry matter, organic matter and ether extract, and the better responses were obtained supplementing 0.194, 0.185 and 0.188 %. Quadratic effects were observed for the nitrogen excreted in feces, total nitrogen excretion, nitrogen efficiency use and nitrogen digestibility. The inulin levels of 0.194 and 0.216 %, in piglet diets, were the better for dry matter digestibility and total nitrogen excretion, respectively.

Balanço de nitrogênio em viveiros de crescimento final de Macrobrachium amazonicum com uso de aeração noturna e substratos artificiais

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeito de dietas e altura da coluna d’água na sobrevivência de larvas de Betta splendens e o aporte de nitrogênio e fósforo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)