Qualidade de pitaias de polpa branca armazenadas em diferentes temperaturas

The aim of this paper was to evaluate the effect of storage temperature on the quality of red pitaya of pulp white, produced in Itajobi city, São Paulo state. The pitayas were stored at room temperature, (21-27 degrees C with 44-63% de UR), at 18 +/- 1 degrees C, with 86-92% RH), 13 +/- 1 degrees C, with 85-90% RH and at 8 +/- 1 degrees C, with 85-95% RH. The quality was monitored during storage time through the parameters: fresh weight loss titleble acidity; soluble solids contents; vitamin C, external appearance, pH and fruit firmness. Through the results obtained may be concluded that the temperature at 8 +/- 1 degrees C it was proportioned the small fresh weight loss; the acidity, soluble solids, pH and fruit firmness were influenced by the storage temperature and storage time, but the temperature at 8 +/- 1 degrees C it was that occasioned the small change theses parameters. In general, it can be concluded that the temperature at 8 +/- 1 degrees C it was the best to maintenance the quality of pitaya fruit.

Cytotoxicity of denture base resins: Effect of water bath and microwave postpolymerization heat treatments

Purpose: This study compared the effect of two postpolymerization heat treatments on the cytotoxicity of three denture base resins on L929 cells using 3H-thymidine incorporation and MTT assays. Materials and Methods: Sample disks of Lucitone 550, QC 20, and Acron MC resins were fabricated under aseptic conditions and stored in distilled water at 37°C for 48 hours. Specimens were then divided into three groups: (1) heat treated in microwave oven for 3 minutes at 500 W; (2) heat treated in water bath at 55°C for 60 minutes; and (3) no heat treatment. Eluates were prepared by placing three disks into a sterile glass vial with 9 mL of Eagle's medium and incubating at 37°C for 24 hours. The cytotoxic effect from the eluates was evaluated using the 3H-thymidine incorporation and MTT assays, which reflect DNA synthesis levels and cell metabolism, respectively. Results: The components leached from the resins were cytotoxic to L929 cells when 3H- thymidine incorporation assay was employed. In contrast, eluates from all resins revealed noncytotoxic effects as measured by MTT assay. For both MTT assay and 3H-thymidine incorporation, the heat treatments did not decrease the cytotoxicity of the materials tested. Conclusion: Resins were graded by 3H-thymidine incorporation assay as slightly cytotoxic and by MTT assay as noncytotoxic. Cytotoxicity of the denture base materials was not influenced by microwave or water bath heat treatment.

Shear bond strength of adhesive systems to enamel and dentin. Thermocycling influence

Objectives: The purpose of the this study was to evaluate the influence of thermocycling on shear bond strength on bovine enamel and dentin surfaces of different adhesive systems. Methods: Thirty sound bovine incisors were sectioned in mesiodistal and inciso-cervical direction obtaining 60 incisal surfaces (enamel) and 60 cervical surfaces (dentin). Specimens were randomly assigned to 3 groups of equal size (n = 40), according to the adhesive system used: I-Single Bond; II-Prime & Bond NT/NRC; III-One Coat Bond. After 24-h storage in distilled water at 37 o C, each main group was divided into two subgroups: A- specimens tested after 24 h storage in distilled water at 37°C; B - specimens submitted to thermocycling (500 cycles). Shear bond strength tests were performed. Data were submitted to ANOVA and Tukey test. Results: Means (MPa) of different groups were: I-AE-16.96, AD-17.46; BE-21.60, BD-12.79; II-AE-17.20, AD-11.93; BE-20.67, BD-13.94; III-AE-25.66, AD-17.53; BE-24.20, BD-19.38. Significance: Thermocycling did not influence significantly the shear bond strength of the tested adhesive systems; enamel was the dental substrate that showed larger adhesive strength; One Coat Bond system showed the best adhesive strength averages regardless of substrate or thermocycling. © 2005 Springer Science + Business Media, Inc.

Minimally processed guava Fruits (Psidium guaiava L.)

'Paluma' guavas, after internal quality evaluation using magnetic resonance tomography, were used to produce fresh-cut product. Fruits were peeled or not, cut in halves and seed removed, and they were packaged in polystyrene trays covered with PVC film or in a PET container with a lid. These packages were stored for 12 days at 5°C, 10°C and ambient temperature (22.6°C). Tomography evaluation verified that impacts produced internal bruising with loss of cellular integrity and liquefication of the placenta tissues. Compression was more evident on the pericarp and cutting promoted superficial deformation. Storage temperature affected the weight loss, with fruit packaged in the polystyrene tray having a greater weight loss. The peeling did not influence weight loss. Product stored at 5°C and 10°C for 8 days had low microbial growth (<103 UFC.g-1) and no coliforms. Rapid spoilage and a short shelf life (3-4 days) occurred when the product was stored at ambient temperature. Peeling reduced ascorbic acid concentration and total soluble solids. Use of calcium to protect fresh-cut products was not efficient. Calcium absorption capacity of 'Pedro Sato' guava was tested using 45Ca. Fruits treated with 2% CaCl2, with or without the radioisotope, were divided in four layers (epicarp, mesocarp, endocarp and seed) and analyzed for the total and 45Ca calcium. It was observed that the applied calcium remained in superficial layers of45fruits, which was confirmed by autoradiography. Internal layers did not contain 45Ca, indicating that calcium was not distributed into different parts of the fruit.

A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis)

The invasive fire ant Solenopsis invicta is medically important because its venom is highly potent. However, almost nothing is known about fire ant venom proteins because obtaining even milligram-amounts of these proteins has been prohibitively challenging. We present a simple and fast method of obtaining whole venom compounds from large quantities of fire ants. For this, we separate the ants are from the nest soil, immerse them in dual-phase mixture of apolar organic solvent and water, and evaporate each solvent phase in separate. The remaining extract from the aqueous phase is largely made up of ant venom proteins. We confirmed this by using 2D gel electrophoresis while also demonstrating that our new approach yields the same proteins obtained by other authors using less efficient traditional methods. © 2013 Elsevier Ltd.

Lentinula edodes (Shiitake) modulates chemically induced mutagenesis by enhancing pitting

This study was undertaken to understand how Lentinula edodes modulates in vivo mutagenesis induced by alkylating agents in bone marrow and peripheral blood as described in our previous article. Male Swiss mice were pretreated for 15 consecutive days with aqueous extracts prepared from L. edodes, after which, the number of circulating blood cells, normal erythroid bone marrow cell cycling, and phagocytosis of micronucleated reticulocyte (MNRET) and activation of spleen macrophages were assessed. The results indicate that the antimutagenicity seen in bone marrow and peripheral blood is exerted by distinct compounds with different actions. The antimutagenic effect in bone marrow is exerted by compounds subject to degradation at deep-freeze storage temperature of -20 C. On the other hand, compounds responsible for antimutagenicity in peripheral blood are not subject to degradation at -20 C. The results also indicate that the antimutagenic action in peripheral blood leading to the reduction of circulating MNRET occurs in the spleen primarily through a phagocytic activity due to higher macrophage numbers and probably not due to the enhanced activation state of individual cells. © Mary Ann Liebert, Inc.

Efeitos da aplicação de salmoura com plasma bovino em biceps femoris (coxão duro) injetado, cru e cozido na estabilidade física, química, microbiológica e sensorial

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Podisus nigrispinus (Dallas, 1851) (Hemiptera: Pentatomidae): efeito do alimento e da temperatura de armazenamento de ovos no desenvolvimento do inseto

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Suplementação de ração de codornas com vitaminas A, D e E sobre o desempenho e qualidade dos ovos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Processamento mínimo de figo-da-índia: formas de preparo, associadas a diferentes embalagens e temperaturas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Conservação de abacaxi e mamão minimamente processados: associação entre o preparo, a embalagem e a temperatura de armazenamento

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Qualidade de caqui armazenado sob refrigeração: estádios de maturação, destanização e irradiação ionizante

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Qualidade e conservação de banana nanica irradiada, climatizada e refrigerada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Antioxidante em vegetais pós-colheira de origem orgânica

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Ciclo de cultivo e técnicas pós-colheita de yacon (Polymnia sonchifolia Poep. Endl.) em função do conteúdo de frutose total nos órgãos subterrâneos

Pós-graduação em Agronomia (Horticultura) - FCA