Artificial neural network associated to UV/Vis spectroscopy for monitoring bioreactions in biopharmaceutical processes

Currently, mammalian cells are the most utilized hosts for biopharmaceutical production. The culture media for these cell lines include commonly in their composition a pH indicator. Spectroscopic techniques are used for biopharmaceutical process monitoring, among them, UV–Vis spectroscopy has found scarce applications. This work aimed to define artificial neural networks architecture and fit its parameters to predict some nutrients and metabolites, as well as viable cell concentration based on UV–Vis spectral data of mammalian cell bioprocess using phenol red in culture medium. The BHK-21 cell line was used as a mammalian cell model. Off-line spectra of supernatant samples taken from batches performed at different dissolved oxygen concentrations in two bioreactor configurations and with two pH control strategies were used to define two artificial neural networks. According to absolute errors, glutamine (0.13 ± 0.14 mM), glutamate (0.02 ± 0.02 mM), glucose (1.11 ± 1.70 mM), lactate (0.84 ± 0.68 mM) and viable cell concentrations (1.89 105 ± 1.90 105 cell/mL) were suitably predicted. The prediction error averages for monitored variables were lower than those previously reported using different spectroscopic techniques in combination with partial least squares or artificial neural network. The present work allows for UV–VIS sensor development, and decreases cost related to nutrients and metabolite quantifications.

Comparison of logistic and neural network models to fit to the egg production curve of White Leghorn hens

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Automatic detection of thermal damage in grinding process by artificial neural network

Esse trabalho tem por objetivo o desenvolvimento de um sistema inteligente para detecção da queima no processo de retificação tangencial plana através da utilização de uma rede neural perceptron multi camadas, treinada para generalizar o processo e, conseqüentemente, obter o limiar de queima. em geral, a ocorrência da queima no processo de retificação pode ser detectada pelos parâmetros DPO e FKS. Porém esses parâmetros não são eficientes nas condições de usinagem usadas nesse trabalho. Os sinais de emissão acústica e potência elétrica do motor de acionamento do rebolo são variáveis de entrada e a variável de saída é a ocorrência da queima. No trabalho experimental, foram empregados um tipo de aço (ABNT 1045 temperado) e um tipo de rebolo denominado TARGA, modelo ART 3TG80.3 NVHB.

Discoloration process modeling by neural network

The photo-oxidation of acid orange 52 dye was performed in the presence of H2O2, utilizing UV light, aiming the discoloration process modeling and the process variable influence characterization. The discoloration process was modeled by the use of feedforward neural network. Each sample was characterized by five independent variables (dye concentration, pH, hydrogen peroxide volume, temperature and time of operation) and a dependent variable (absorbance). The neural model has also provided, through Garson Partition coefficients and the Pertubation method, the independent variable influence order determination. The results indicated that the time of operation was the predominant variable and reaction mean temperature was the lesser influent variable. The neural model obtained presented coefficients of correlation on the order 0.98, for sets of trainability, validation and testing, indicating the power of prediction of the model and its character of generalization. (c) 2007 Elsevier B.V. All rights reserved.

Multinodal Load Forecasting in Power Electric Systems using a Neural Network with Radial Basis Function

In this paper we present the results of the use of a methodology for multinodal load forecasting through an artificial neural network-type Multilayer Perceptron, making use of radial basis functions as activation function and the Backpropagation algorithm, as an algorithm to train the network. This methodology allows you to make the prediction at various points in power system, considering different types of consumers (residential, commercial, industrial) of the electric grid, is applied to the problem short-term electric load forecasting (24 hours ahead). We use a database (Centralised Dataset - CDS) provided by the Electricity Commission de New Zealand to this work.

Short-Term Multinodal Load Forecasting Using a Modified General Regression Neural Network

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Identification of lubricant contamination by biodiesel using vibration analysis and neural network

Purpose - The purpose of this paper is to provide information on lubricant contamination by biodiesel using vibration and neural network.Design/methodology/approach - The possible contamination of lubricants is verified by analyzing the vibration and neural network of a bench test under determinated conditions.Findings - Results have shown that classical signal analysis methods could not reveal any correlation between the signal and the presence of contamination, or contamination grade. on other hand, the use of probabilistic neural network (PNN) was very successful in the identification and classification of contamination and its grade.Research limitations/implications - This study was done for some specific kinds of biodiesel. Other types of biodiesel could be analyzed.Practical implications Contamination information is presented in the vibration signal, even if it is not evident by classical vibration analysis. In addition, the use of PNN gives a relatively simple and easy-to-use detection tool with good confidence. The training process is fast, and allows implementation of an adaptive training algorithm.Originality/value - This research could be extended to an internal combustion engine in order to verify a possible contamination by biodiesel.

Artificial neural networks: A novel approach to analysing the nutritional ecology of a blowfly species, Chrysomya megacephala

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Classification and characterization of places for mapping of environment using hierarchical neural network and omnivision

Mobile robots need autonomy to fulfill their tasks. Such autonomy is related whith their capacity to explorer and to recognize their navigation environments. In this context, the present work considers techniques for the classification and extraction of features from images, using artificial neural networks. This images are used in the mapping and localization system of LACE (Automation and Evolutive Computing Laboratory) mobile robot. In this direction, the robot uses a sensorial system composed by ultrasound sensors and a catadioptric vision system equipped with a camera and a conical mirror. The mapping system is composed of three modules; two of them will be presented in this paper: the classifier and the characterizer modules. Results of these modules simulations are presented in this paper.

Estimation of the volumetric oxygen tranfer coefficient (KLa) from the gas balance and using a neural network technique

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Design of a simplified neuro-fuzzy system based on CMAC Architectures

This work presents the design of a fuzzy controller with simplified architecture that use an artificial neural network working as the aggregation operator for several active fuzzy rules. The simplified architecture of the fuzzy controller is used to minimize the time processing used in the closed loop system operation, the basic procedures of fuzzification are simplified to maximum while all the inference procedures are computed in a private way. As consequence, this simplified architecture allows a fast and easy configuration of the simplified fuzzy controller. The structuring of the fuzzy rules that define the control actions is previously computed using an artificial neural network based on CMAC Cerebellar Model Articulation Controller. The operational limits are standardized and all the control actions are previously calculated and stored in memory. For applications, results and conclusions several configurations of this fuzzy controller are considered.

An application of neural network technique to correct the dome temperature effects on pyrgeometer measurements

This work describes an application of a multilayer perceptron neural network technique to correct dome emission effects on longwave atmospheric radiation measurements carried out using an Eppley Precision Infrared Radiometer (PIR) pyrgeometer. It is shown that approximately 7-month-long measurements of dome and case temperatures and meteorological variables available in regular surface stations (global solar radiation, air temperature, and air relative humidity) are enough to train the neural network algorithm and correct the observed longwave radiation for dome temperature effects in surface stations with climates similar to that of the city of São Paulo, Brazil. The network was trained using data from 15 October 2003 to 7 January 2004 and verified using data, not present during the network-training period, from 8 January to 30 April 2004. The longwave radiation values generated by the neural network technique were very similar to the values obtained by Fairall et al., assumed here as the reference approach to correct dome emission effects in PIR pyrgeometers. Compared to the empirical approach the neural network technique is less limited to sensor type and time of day (allows nighttime corrections).

Feedforward neural networks based on PPS-wavelet activation functions

Function approximation is a very important task in environments where computation has to be based on extracting information from data samples in real world processes. Neural networks and wavenets have been recently seen as attractive tools for developing efficient solutions for many real world problems in function approximation. In this paper, it is shown how feedforward neural networks can be built using a different type of activation function referred to as the PPS-wavelet. An algorithm is presented to generate a family of PPS-wavelets that can be used to efficiently construct feedforward networks for function approximation.

Optimization of Radial Basis Function neural network employed for prediction of surface roughness in hard turning process using Taguchi's orthogonal arrays

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Neural network detection of grinding burn from acoustic emission

An artificial neural network (ANN) approach is proposed for the detection of workpiece `burn', the undesirable change in metallurgical properties of the material produced by overly aggressive or otherwise inappropriate grinding. The grinding acoustic emission (AE) signals for 52100 bearing steel were collected and digested to extract feature vectors that appear to be suitable for ANN processing. Two feature vectors are represented: one concerning band power, kurtosis and skew; and the other autoregressive (AR) coefficients. The result (burn or no-burn) of the signals was identified on the basis of hardness and profile tests after grinding. The trained neural network works remarkably well for burn detection. Other signal-processing approaches are also discussed, and among them the constant false-alarm rate (CFAR) power law and the mean-value deviance (MVD) prove useful.