Medicinal plants' hosting ability for nematode meloidogyne javanica and m. incognita

Baida, F.C., D.C. Santiago, L. H. I. Vidal, L. C. Baida, C. T. Stroze. 2011. Medicinal Plants' Hosting Ability for Nematode Suitability Meloidogyne incognita and M. javanica. Nematropica 41: 150-153.Medicinal plants can be attacked by pests, diseases and nematodes, which can compromise the quality and quantity of their healing properties and their yield. The aim of this study was to analyse the reaction of 15 medicinal plant species to the nematode Meloidogyne spp. The seedlings were produced by seed germination or cuttings under greenhouse conditions. A completely random experimental pattern of 15 treatments and 10 replications was chosen for the study. The seedlings were inoculated with approximately 5000 eggs + J(2)/plant 20 days after planting. Plant height and fresh and dry leaf weight, were measured 60 days after planting. The roots were collected, thoroughly washed and stained with Philoxine B and then processed to extract the eggs to determine the reproduction factor. For M. incognita the results showed that Chamomile was susceptible with RF = 1,64 making it a good host, and the other plants were resistant (RF < 1), and for M. javanica that all the plants showed resistance (RF < 1), Myrrh, Rue and Balsam demonstrating immunity (RF = 0).

Novos Registros sobre Meloidogyne mayaguensis no Brasil e Estudo Morfológico Comparativo com M. incognita

Almeida, E.J., P.L.M. Soares, A.R. Silva & J.M. Santos. 2008. New records on Meloidogyne mayaguensis in Brazil and comparative study with M. incognita. Meloidogyne mayaguensis is the plant-parasitic nematode responsible for a great impact on guava production in Brazil, since it has been the cause for eradication of thousand of hectares of guava plantations in the Northeastern region. In the present study, this species was detected in soybean fields of Ituverava municipality, São Paulo state, and in different vegetable crops (lettuce, cucumber, pepper and cherry tomato) in Chapada dos Guimaraes municipality, Mato Grosso state, causing root galls and other symptoms. The species was identified on the basis of the perineal pattern of females, and on the morphology and morphometry of anterior region of males. Isozyme phenotype for esterase was used for confirmation. This constitutes the first report on the occurrence of M. mayaguensis on lettuce, cucumber, pepper and cherry tomato cultures in Mato Grosso state and the first one in soybean in São Paulo state. It was found that morphological features of male anterior region and female perineal pattern are enough for the safe distinction between M. mayaguensis and M. incognita.

Avaliação Comparativa da Agressividade de Meloidogyne javanica e M. incognita à Variedade SP 911049 de Cana-de-açúcar

Nematodes cause extensive losses to sugarcane in Brazil and also in other producing regions. Meloidogyne incognita, M. javanica and Pratylenchus zeae are the key species for this culture worldwide. In the present study, the aggressiveness of M. javanica and M. incognita to sugarcane variety SP 911049 was evaluated comparatively,. The following parameters were compared: reproduction factor (RF) of these nematodes, effect of nematodes in the natural incidence of pests, and the influence on the development and technological characteristics of sugarcane. Considering the data of RF, biometrics, natural infestation of pests, mortality of plants, and technological variables, it was concluded that M. javanica was more aggressive to sugarcane, although its rate of multiplication was much smaller than the one of M. incognita.

Reprodução de Meloidogyne incognita, M. javanica e Pratylenchus coffeae em Diferentes Cultivares de Bananeira

Banana (Musa spp.) is one of the most consumed fruits in the world, and it is cultivated in many tropical countries. In Brazil, the productivity is low due to several factors such as the incidence of pests and diseases. The nematodes are among the most important problems found in the production of bananas. This research studied the reproduction of Meloidogyne incognita race 2, M. javanica and Pratylenchus coffeae in different cultivars of banana. The evaluation of the experiments with Meloidogyne spp. was carried out 120 days after the inoculation. The analyzed parameters were: number of gall, number of the external masses of eggs, number of eggs per gram of root, reproduction factor and number of juveniles in the soil. The evaluation of the experiment with P. coffeae was carried out 90 days after the inoculation; the final population was evaluated. The cultivars PV-0344, Maca, Grande Naine, FHIA 01, Thap Maeo and Prata Ana allowed greater multiplication of P. coffeae than Caipira, SH-3640 and FHIA-18. For M. javanica, the cultivars Calypso, Bucanneer, Grande Naine, PV-0344, Nanicao Magario, FHIA-02, SH-3640, Pacovan, and Prata Ana exhibited larger populations, decreasing in Maca. All cultivars allowed high multiplication rates of M. incognita.

Flutuação populacional de Meloidogyne enterolobii em pomar de goiabeira (Psidium guajava)

The objective of this work was to study the population fluctuation of Meloidogyne enterolobii in a commercial orchard of 'Paluma' guava with five years of age, under micro sprinkler irrigation system and usual practices for this crop. In the orchard, located in the municipality of Vista Alegre do Alto (SP) Brazil and infested by M. enterolobii, five plants exhibiting nematode symptoms were selected for monthly sampling from July 2007 to June 2008. Two single samples of rhizosphere soil and guava roots were collected from each plant, with the aid of hoe to a depth of 20 cm on the projection of the trees, forming a composite sample per plant. In nematology laboratory of Faculdade de Ciencias Agrarias e Veterinarias Universidade Estadual Paulista, eggs and second stage juveniles (J2) of M. enterolobii were extracted from the samples and quantified. There was not a marked seasonal variation in the nematode population in soil or roots, possibly as a result of the guava vegetation stimulated by irrigation or the presence of invasive plants during the experimental period. However, the soil density reached a peak in January, corresponding to the month of elevated rainfall and temperatures. In roots, there were two significant peaks, in February and a major peak in August. There was a negative correlation between nematode populations in soil and roots.

Duration of Protopolybia exigua exigua (de Saussure) nests and related aspects as influenced by phorid fly infestation (vespidae, polistinae, epiponini)

The main characteristics of 55 nests of Protopolybia exigua exigua were periodically evaluated in Ribeirão Preto, southeastern Brazil. Although the queens' life-span can eventually reach one year, nests barely thrive for six months because most of them are periodically invaded by the scavenger phorid fly, Megaselia aff. picta. Under heavy infestation the adult wasp population partially closes the nest entrance, migrates and constructs a new nest in the neighborhood. Afterwards the old-nest's entrance can be completely closed by the wasps which results in blocking the parasitoid flies inside. In the tropics, predation by ants is considered the main selective pressure which shaped most of the bionomic characteristics of Polistinae wasps. It is suggested that Megaselia can easily overcome the usual wasps defensive tactics just because they are so specialized against ants. Aerial raids, fast erratic-jerking movements, rapid and efficient oviposition behavior, etc. can eventually turn Megaselia into a very important enemy of some social wasps.

Foraging activity of Protopolybia exigua (Hymenoptera, Vespidae) in different phases of the colony cycle, at an area in the region of the Médio São Francisco River, Bahia, Brazil

In this work, the foraging activity of Protopolybia exigua (de Saussure) was studied, aiming to verify the amplitude of the foraging time, the relation between the physical factors of weather (temperature, relative air humidity, luminosity and wind speed) and the daily activity of searching for resources, as well as to analyze the influence of the colony development phases and the number of individuals (adults and immatures) engaged in such activity. The study was carried out in two areas of the city of Bom Jesus da Lapa, Bahia, Brazil, during the period from January to June 2006, when the observation of 12 colonies of P. exigua was performed. The results revealed this species presented an amplitude of activity of almost 13 hours. There was an average of 44.4 nest exits and 37.6 returns a day by the nest workers and the Returns with Resources Index for this species was 93.5%. The foraging activity increased as of 10:01, and became more intense, with the peak from 1:01 p.m. to 4:00 p.m., when the highest temperatures (°C) and the lowest values of relative air humidity (%) were observed. The colonies that had a larger number of larvae presented a higher average number of trips to the field per hour (10.94) and the number of females in the colony tends to induce foraging activity. There were no differences between the numbers of nest exits by the workers regarding the three phases of colony development.

Resistance of rose rootstocks to Meloidogyne hapla nematode

Roses are widely used in landscaping. One of the most important fitossanitary problems of this ornamental plant in gardens is the fitonematodes, especially Meloidogyne hapla, which is the most important one in colder climate regions. This work had the objective of study the resistance of nine rose rootstocks (Rosa multiflora 'Paulista', R. multiflora 'Japanese', R. multiflora 'Iowa', R. multiflora 'Kopman's', R. indica × multiflora, R. indica 'Mayor', R. sp. 'Natual Brier', R. manetti and R. canina 'Inermis') to Meloidogyne hapla nematode. Ten replications of each rootstock were used. The roots, collected in a commercial area of cut roses, visually had galls there were isolated and identified. The inoculum of M. hapla was previously produced in rose and tomatoes seedlings under greenhouse conditions. The plants were harvested ninety days after inoculation. The roots were washed and the number of eggs and juveniles recuperated in the root system of each plant was estimated by the Final Population (FP), and the Reproductive Factor (RF) was calculated. The rootstocks with RF<1 values were considered resistant, and the ones with RF>1, susceptible. Rosa manetti and Rosa sp. 'Natual Brier' rootstocks showed the lowest values for RF, indicating that, although they are susceptible, they provide the lowest nematode reproduction. Based on the RF values, it was concluded that all the rose rootstocks evaluated were susceptible to M. hapla nematode.

Rootstocks resistant to Meloidogyne incognita and compatibility of grafting in net melon

Due to the few studies about grafting in net melon, in order to obtain better control of soil pathogens, the aim of the present study was to evaluate 16 genotypes of Cucurbitaceae: Benincasa hispida, Luffa cylindrica, pumpkin 'Jacarezinho', pumpkin 'Menina Brasileira', squash 'Exposição', squash 'Coroa', pumpkin 'Canhão Seca', pumpkin 'Squash', pumpkin 'Enrrugado Verde', pumpkin 'Mini Paulista', pumpkin 'Goianinha', watermelon 'Charleston Gray', melon 'Rendondo Gaucho', melon 'Redondo Amarelo', cucumber 'Caipira HS' and cucumber 'Caipira Rubi', regarding to compatibility of grafting in net melon and resistance to Meloidogyne incognita, based on the reproduction factor (RF), according to Oostenbrink (1966). To assess resistance, the seedlings were transplanted to ceramic pots and inoculated with 300/mL eggs and/or second stage juveniles of M. incognita. At 50 days after transplanting, the plants were removed from the pots and the resistance was evaluated. The compatibility between resistant rootstock and grafts of net melon was determined by performing simple cleft grafting, in a commercial net melon hybrid of great market acceptance and susceptible to M. incognita (Bonus no. 2). The genotypes Luffa cylindrica, pumpkin 'Goianinha', pumpkin 'Mini-Paulista', melon 'Redondo Amarelo', watermelon 'Charleston Gray' are resistant to the nematode M. incognita. The better compatibilities occurred with the rootstocks melon 'Amarelo', which presented 100% of success, followed by pumpkin 'Mini-Paulista' with 94%. On the other hand, Sponge gourd, watermelon 'Charleston Gray' and pumpkin 'Goianinha' showed low graft take percentages of 66%, 62% and 50%, respectively.

Flutuação populacional de Meloidogyne mayaguensis em pomar de goiabeira e estudo do controle biológico com fungos nematófagos associado a culturas de cobertura

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Respostas biométricas e fotossintética de plantas de milho a Pratylenchus spp. e avaliação da resistência de genótipos de milho e sorgo a esses nematóides e a Meloidogyne spp

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo das inter-relações patógeno-hospedeiro de Meloidogyne incognita (Kofoid & White) Chitwood, M. Javanica (Treub) Chitwood e Pratylenchus brachyurus (Godfrey) Filipjev & Schuurmans Stekhoven em cana-de-açúcar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Eficácia de ácidos húmicos e fúlvicos no controle de Meloidogyne incognita e Meloidogyne javanica in vitro e na cultura de cana-de-açúcar em vasos

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Análise da expressão de genes relacionados à resistência a Meloidogyne javanica em soja, através da técnica de PCR em tempo real

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudos histopatológicos e ciclos biológicos de Meloidogyne mayaguensis e M. javanica em tomateiros com gene Mi

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)