Green chromatographic fingerprinting: An environmentally friendly approach for the development of separation methods for fingerprinting complex matrices

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Educação bilingue para surdos: práticas discursivas em tempos de exclusão

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Nanostructured screen-printed electrodes modified with self-assembled monolayers for determination of metronidazole in different matrices

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Convergência cultural, divergência nos olhares: práticas discursivas e construção de subjetividades na cultura da convergência

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Um estudo sobre as interações discursivas numa aula experimental de Física

This is a study on importance the discourse of the teacher associated with social interactions triggered in classroom from of an experimental activity. We will analyzed a trial class in which a physics teacher of a public school Guaratinguetá city interacted with their student, seeking to categorize certain movements of the teacher's speech and identify the moments when this could establish the definition of the situation, mediation semiotic and intersubjectivity

Stability at different temperatures of turmeric oleoresin encapsulated in maltodextrin/gelatin matrices by freeze-drying

Turmeric (Curcuma longa L.), which has been used for long time as a spice, food preservative and coloring agent, is a rich source of beneficial phenolic compounds identified as curcuminoids. These phenolic compounds are known for their antioxidant, anti-inflammatory and antimutagenic properties, among others. On the other hand, they are very susceptible to oxidation, requiring protection against oxygen, light and heat. This protection can be achieved by microencapsulation. In this work, the characteristics and the stability of turmeric oleoresin encapsulated by freeze-drying using mixtures of maltodextrin and gelatin as wall materials were studied. Encapsulated turmeric oleoresin was stored at –20, 25 and 60 °C, in the absence of light, and analyzed over a period of 35 days for curcumin and total phenolic contents and color. Results showed that the samples produced with 26% maltodextrin/0.6% gelatin and 22% maltodextrin/3% gelatin presented good encapsulation efficiencies and solubility. In general, the method of encapsulation employed originated products with satisfactory thermal stability, although the encapsulated materials with a higher proportion of maltodextrin in relation to gelatin had better stabilities, especially at –20 and 25 °C temperatures.

Produções discursivas do horror: materialidade fílmica e memória na trilogia de Zé do Caixão

Pós-graduação em Linguística e Língua Portuguesa - FCLAR

Youtube: práticas discursivas e identitárias no ciberespaço

Inegavelmente, as mídias tradicionais como a televisão, o rádio e a mídia impressa detêm grande influência na sociedade contemporânea, tanto para o delineamento do pensamento coletivo, quanto para a sugestão à formação cultural por meio de enunciados produzidos por sujeitos que ocupam determinadas formações sócio- ideológicas. Entretanto, atualmente, um objeto que tem ganhado espaço privilegiado, diferente das mídias tradicionais, são as mídias digitais. Fluidos no grande oceano do ciberespaço que se constitui como um aglutinador de mídias, onde se mesclam mídias tradicionais e novas mídias, centros recepto-difusores encontram-se dispersos em toda parte e, efetivamente, em parte alguma, tornando-se, assim, um espaço onde a cultura participativa e (d)a interatividade ganham traços. Dessa maneira, o presente trabalho, embasado principalmente pelo aporte teórico da Análise do Discurso de linha francesa, tendo como fio condutor as reflexões de Michel Foucault, procura discutir questões de identidades produzidas e veiculadas nos meios digitais. Toma-se como corpus de análise vídeos caseiros de pessoas “anônimas” que transmitem-se pelo canal do YouTube, onde mostram suas habilidades vocais, artísticas e até mesmo conhecimentos de técnicas áudio-visuais para a produção de efeitos de sentido. Estes sujeitos fazem uso de técnicas de confissão para a construção e afirmação de uma identidade de “artista” e, por essas práticas, produzem novas subjetividades. Em torno destes vídeos, ainda, são originadas comunidades inteligentes de pertencimento pontuadas no próprio YouTube e em outras plataformas, como no Twitter, Facebook, My Space, etc. onde são mobilizados e compartilhados os savoir-faire desses sujeitos e virtualizadas e atualizadas as identidades à luz de uma cultura da convergência.

As estratégias discursivas e suas implicações na relação entre oralidade e escrita? um estudo do parêntese na crônica

Based on functional theory, the text discusses the relations between spoken language and written language, defending not only the existence of a sole grammatical system for both, but also the relevance of studies contemplating specificities of use, some of these being more directed toward interaction, and others being more directed toward system. The investigation that aims to prove these premises used the chronicle as examination material, due to its linguistic colloquialism in general and due to the chronicler’s personal engagement, which involve linguistic strategies available for the expression of information and for the reader’s understanding. The investigation was concentrated on the discourse parenthesis, especially on its connection with the degree of language planning and with the creation of view plans and the establishment of emphasis. It was concluded that the resource of parenthesis both represents linguistic informality (which is more directed toward speaking) and shows the care with preparation and marking of style (which is more directed toward writing), illustrating specificities.

Do riso à produção de enunciados humorísticos: implicações pragmáticas e discursivas

Volume eletrônico. Número especial. 2a parte

Stability of curcumin microencapsulated by spray and freeze drying in binary and ternary matrices of maltodextrin, gum arabic and modified starch

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Water distribution in dentin matrices: Bound vs. unbound water

Objective. This work measured the amount of bound versus unbound water in completely-demineralized dentin.Methods. Dentin beams prepared from extracted human teeth were completely demineralized, rinsed and dried to constant mass. They were rehydrated in 41% relative humidity (RH), while gravimetrically measuring their mass increase until the first plateau was reached at 0.064 (vacuum) or 0.116 g H2O/g dry mass (Drierite). The specimens were then exposed to 60% RH until attaining the second plateau at 0.220 (vacuum) or 0.191 g H2O/g dry mass (Drierite), and subsequently exposed to 99% RH until attaining the third plateau at 0.493 (vacuum) or 0.401 g H2O/g dry mass (Drierite).Results. Exposure of the first layer of bound water to 0% RH for 5 min produced a -0.3% loss of bound water; in the second layer of bound water it caused a -3.3% loss of bound water; in the third layer it caused a -6% loss of bound water. Immersion in 100% ethanol or acetone for 5 min produced a 2.8 and 1.9% loss of bound water from the first layer, respectively; it caused a -4 and -7% loss of bound water in the second layer, respectively; and a -17 and -23% loss of bound water in the third layer. Bound water represented 21-25% of total dentin water. Chemical dehydration of water-saturated dentin with ethanol/acetone for 1 min only removed between 25 and 35% of unbound water, respectively.Signcance. Attempts to remove bound water by evaporation were not very successful. Chemical dehydration with 100% acetone was more successful than 100% ethanol especially the third layer of bound water. Since unbound water represents between 75 and 79% of total matrix water, the more such water can be removed, the more resin can be infiltrated. (C) 2014 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

A hybrid method for the solution of a sparse power system matrices on vector computers

This paper describes a methodology for solving a linear system of equations on vector computer. The methodology combines direct and inverse factors. The decomposition and implementation of the direct solution in a CRAY Y-MPZE/232, and the performance results are discussed.

Characterization of biomodified dentin matrices for potential preventive and reparative therapies

Biomodification of existing hard tissue structures, specifically tooth dentin, is an innovative approach proposed to improve the biomechanical and biochemical properties of tissue for potential preventive or reparative therapies. The objectives of the study were to systematically characterize dentin matrices biomodified by proanthocyanidin-rich grape seed extract (GSE) and glutaraldehyde (GD). Changes to the biochemistry and biomechanical properties were assessed by several assays to investigate the degree of interaction, biodegradation rates, proteoglycan interaction, and effect of collagen fibril orientation and environmental conditions on the tensile properties. The highest degree of agent–dentin interaction was observed with GSE, which exhibited the highest denaturation temperature, regardless of the agent concentration. Biodegradation rates decreased remarkably following biomodification of dentin matrices after 24 h collagenase digestion. A significant decrease in the proteoglycan content of GSE-treated samples was observed using a micro-assay for glycosaminoglycans and histological electron microscopy, while no changes were observed for GD and the control. The tensile strength properties of GD-biomodified dentin matrices were affected by dentin tubule orientation, most likely due to the orientation of the collagen fibrils. Higher and/or increased stability of the tensile properties of GD- and GSE-treated samples were observed following exposure to collagenase and 8 months water storage. Biomodification of dentin matrices using chemical agents not only affects the collagen biochemistry, but also involves interaction with proteoglycans. Tissue biomodifiers interact differently with dentin matrices and may provide the tissue with enhanced preventive and restorative/reparative abilities.