Comparison of two methods of synchronization of estrus in brown brocket deer (Mazama gouazoubira)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Seasonal variation in growth and yield of the brown mussel Perna perna (L.) cultured in Ubatuba, Brazil

Seasonal variation in growth and yield of cultured brown mussels Perna perna was studied in Ubatuba, south-east Brazilian coastline. Young mussels were transplanted (seeded) into four groups of 16 polyethylene net tubes 2 m long, suspended from a wooden raft (18 m 2). The first group was set out in April (autumn), the next in July (winter), the next in October (spring) 1984 and the final group in January (summer) 1985. One net tube of each group was sampled monthly and biometric data were collected. Growth was initially faster for the spring group, but at the end of the culture period length and weight were not statistically different between groups. L(∞) and W(∞) were 73.9, 71.3, 72.7 and 73.8 mm and 26.3, 23.9, 25.7 and 25.7 g for the autumn, winter, spring and summer groups, respectively. Maximum yield was attained 9 months after seeding for groups of autumn, winter and spring (7.2, 5.2 and 6.3 kg x m -1, respectively) and after 10 months for the summer group (6.9 kg X m -1). The conclusion of this study is that, growth and yield were unaffected by the season of seeding at the study site and that it is not commercially worthwhile to farm mussels more than 9 months, due to yield decrease.

Continuous emission versus freeze-out via hanbury brown-twiss

The effect of the continuous emission hypothesis on the two-pion Bose-Einstein correlation function is discussed and compared with the corresponding results based on the usual freeze-out. Sizable differences in the correlation function appear in these different descriptions of the decoupling process. This means that, when extracting properties of the hot matter formed in high-energy heavy-ion collisions from the data, completely different conclusions may be reached according to the description of the particle emission process adopted.

Efeito da sacarose e sorbitol na conservação in vitro de Passiflora giberti N. E. Brown

This work objectified the study of sucrose and sorbitol effect in the in vitro conservation for Passiflora giberti N. E. Brown, access. Therefore, an experiment was conducted in a completely randomized design to compare control treatment (standard MS) to MS medium supplemented with three sucrose concentrations (0, 15 and 30 g L -1) combined with three sorbitol concentrations (10, 20 and 40 g L -1), in a total of 10 treatments with 20 replicas. The experiment evaluation was carried out at 30, 60, 90 and 120 days of incubation, whereas the height of shoots (cm), number of roots, number and color of leaves were observed. The results showed the possibility to maintain passion-fruit microplants for a four months period under slow growth in MS medium supplemented with 10 or 20 g L -1 of sorbitol, without sucrose, and kept under 16 hours photoperiod (22 μ E m -2 s -1) and temperature of 27 ± 1°C. Sucrose sustained the longest development of the microplants. Root formation was affected by the sorbitol in the concentration of 40 g L -1 and by the absence of sucrose in the culture medium.

Ophthalmic patterns of captive brown brocket deer (Mazama gouazoubira)

Captive brown brocket deer (Mazama gouazoubira) were manually restrained to assess tear production by the Schirmer tear test I to measure intraocular pressure by applanation tonometry, to examine ocular conjunctival epithelial cells via cytologic and histologic samples, and to survey ocular conjunctival microflora by microbiologic culture. The mean value for the Schirmer tear test I was 8.9 ± 1.8 mm/min, and the mean intraocular pressure was 15.3 ± 3.1 mm Hg. Conjunctival epithelium contained stratified pavimentous layers of cells, and the microflora consisted of predominantly gram-positive bacteria. Copyright 2007 by American Association of Zoo Veterinarians.

Modelación para ajuste de la curva de lactación usando función lineal, no lineal y polinomios segmentados en ganados con alto grado de sangre Brown Swiss

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Unusually rapid growth of brown tumour in the mandible after parathyroidectomy associated with the presence of a supernumerary parathyroid gland

The aim of this study is to report the case of a quick growing brown tumour in the jaw after a parathyroidectomy due to the presence of a rare fifth parathyroid gland. The patient had chronic renal disease and the diagnosis was tertiary hyperparathyroidism. Thirty days after the parathyroidectomy, the patient returned with a significant increase in the tumour size. The suspicion of a supernumerary gland was confirmed by parathyroid scintigraphy. The treatment of brown tumour is dependent on the treatment of the hyperparathyroidism. However, curettage should be considered if a large lesion is disturbing mastication. In conclusion, this case should attract the attention of general practitioner dentists, since they may be the first professionals who have contact with the patient with a brown tumour in the jaws. Likewise, this case emphasises the importance of knowing the type of hyperparathyroidism involved to allow for effective treatment planning. © 2011 European Association for Cranio-Maxillo-Facial Surgery.

Cytogenetic description of the Amazonian brown brocket Mazama nemorivaga (Artiodactyla, Cervidae)

The Amazonian brown brocket Mazama nemorivaga (Cuvier, 1817) is a small to medium-sized deer from the Amazon rainforest and ecotones. The first karyotype described was 2n=67 to 69 + 2-7 B and FN= 69-72, in which all chromosomes were acrocentric and the X chromosome was the only submetacentric chromosome. However, important aspects of the species chromosome evolution were not resolved because of the lack of information on chromosome banding. The G-banding pattern of M. nemorivaga karyotype showedthe presence of an XX/XY1Y2 sex chromosome system as a product of an X-autosome tandem fusion, which results in a basic 2n=68, FN=70 in females and 2n= 69, FN=70 in males. The fact that this karyotype only differs from that of Capreolus capreolus pygargus (Pallas, 1771; 2n=70, FN=72+B) by X-autosome tandem fusion may corroborate the basal condition of M. nemorivaga and its proximity to the ancestral karyotype of the American Odocoileini. A derived karyotype 2n=67, XY1Y2, FN=70 + 3B from the Brazilianstate of Mato Grosso (the western Amazon) may be evidence of differentiation between western and eastern populations. © Bruno Ferreto Fiorillo et al.

Influence of densities on the growth of brown mussel Perna perna L. cultivated in suspended socks at Caraguatatuba, southeastern Brazil

This study was carried out in a marine area at Cocanha Beach, Caraguatatuba, southeastern Brazil, aiming to determine the most appropriated density for brown mussel (Perna perna L.) cultured in suspended socks (French System), in order to reduce costs with acquisition of juvenile mussels (seed) and labor. In September 2007, 15 socks measuring 2 m each were reared at densities of 300, 400 and 500 seeds/m. After 250 days, mussels were harvested and separated in two length classes: non-commercial (<6 cm) and commercial (≥6 cm). Mussels were counted and weighed. In August 2008, the experiment was repeated with 1-m-long socks at lower densities (200, 250 and 300 seeds/m) in order to identify differences among commercial-sized mussels only. Data were analyzed by ANOVA or nonparametric Kruskal-Wallis test, followed by Tukey or SNK tests, respectively. The results suggest that it is feasible to use densities from 250 to 300 seeds/m, since biomass yields are similar to those registered at higher densities and the use of mussel seed is therefore optimized. © 2013 Springer Science+Business Media Dordrecht.

Evaluation of estrogenic, antiestrogenic and genotoxic activity of nemorosone, the major compound found in brown Cuban propolis

Background: Brown propolis is the major type of propolis found in Cuba; its principal component is nemorosone, the major constituent of Clusia rosea floral resins. Nemorosone has received increasing attention due to its strong in vitro anti-cancer action. The citotoxicity of nemorosone in several human cancer cell lines has been reported and correlated to the direct action it has on the estrogen receptor (ER). Breast cancer can be treated with agents that target estrogen-mediated signaling, such as antiestrogens. Phytoestrogen can mimic or modulate the actions of endogenous estrogens and the treatment of breast cancer with phytoestrogens may be a valid strategy, since they have shown anti-cancer activity.Methods: The aim of the present investigation was to assess the capacity of nemorosone to interact with ERs, by Recombinant Yeast Assay (RYA) and E-screen assays, and to determine by comet assay, if the compound causes DNA-damaging in tumoral and non-tumoral breast cells.Results: Nemorosone did not present estrogenic activity, however, it inhibited the 17-β-estradiol (E2) action when either of both methods was used, showing their antiestrogenicity. The DNA damage induced by the benzophenone in cancer and normal breast cells presented negative results.Conclusion: These findings suggest that nemorosone may have therapeutic application in the treatment of breast cancer. © 2013 Camargo et al.; licensee BioMed Central Ltd.

Construção de filmes ultra finos de Bismarck Brown R por eletropolimerização e sua aplicação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Fontes e modos de aplicação de potássio na alface americana (Lactuca sativa cv. Lucy Brown) em ambiente protegido

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of sex and seasons of the year on hematologic and serum biochemical variables of captive brown brocket deer (Mazama gouazoubira)

The Brown brocket deer (Mazama gouazoubira) is the most common free-living and captive deer in South America, especially in Brazil, and has great ecological and scientific significance. However, data on hematological and biochemical parameters in brown brocket deer are scarce. The goal of this study was to establish reference ranges for hematological and biochemical parameters of Mazama gouazoubira, comparing differences during the seasons of the year and between sex. Blood samples from ten adult healthy brown brocket deer (6 female and 4 male) were collected during daytime, monthly, during 12 months. The animals were maintained in individual stable, protected from noise and fed ad libitum with commercial ration and green fodder. For blood collection, animals were submitted to physical restrain for no longer than 2 minutes. The following parameters were determined: red blood cell count (RBC), haemoglobin concentration, packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), white blood cell count (WBC), platelet count, enzyme activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (GGT) and serum levels of alkaline phosphatase (ALP), creatine kinase (CK), total protein (TP), albumin, cholesterol, total calcium, ionic calcium, sodium, potassium, magnesium, triglycerides, creatinine and urea. Values were compared according to season and sex. RBC count, WBC count and MCV suggested seasonal influence. Haemoglobin concentration, PCV and MCV were influenced by sex. Serum concentration of total calcium, ionic calcium, sodium, potassium and magnesium were influenced by season. Serum magnesium was also influenced by sex. The blood parameters herein reported may be useful as reference values for diagnostic and prognostic purposes in captive brown-brocket deer.