Detec����o de anticorpos anti-leishmania chagasi em c��es do munic��pio S��o Jos�� do Rio Preto, S��o Paulo

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Estudo cl��nico-laboratorial das articula����es de c��es naturalmente infectados com leishmaniose visceral e experimentalmente inoculados com Leishmania chagasi por via intra-articular

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Detec����o das metaloproteinases-2 e -9 no plexo cor��ide e no liquor de c��es naturalmente infectados por Leishmania chagasi

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Avalia����o do fator de transforma����o do crescimento-beta 1, interleucina-10 e interferon-gama em c��es machos, assintom��ticos e sintom��ticos, naturalmente infectados por Leishmania (Leishmania) chagasi

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Necessidade de vigil��ncia epidemiol��gica para Leishmania infantum (syn. Leishmania chagasi) e Leishmania (Viannia) braziliensis em flebotom��neos e gatos errantes no Bosque Municipal de Mar��lia-SP

P��s-gradua����o em Medicina Veterin��ria - FMVZ

Infec����o por Leishmania chagasi em gatos com dermatopatias provenientes de ��rea endemica para Leishmaniose visceral

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Resposta humoral, carga parasit��ria e avalia����o histopatol��gica na infec����o experimental por Leishmania (Leishmania) chagasi em camundongos BALB/c imunossuprimidos

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

N��veis de TNF-alfa, IL-4 e IL-10 no ba��o e no f��gado de c��es naturalmente infectados por Leishmania (Leishmania) chagasi

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Resposta imune ap��s �� infec����o por Leishmania chagasi em camundongos Balb/c imunossuprimidos

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Serological evidence of Infection by Leishmania (Leishmania) infantum (Synonym: Leishmania (Leishmania) chagasi) in free-ranging wild mammals in a nonendemic region of the state of S��o Paulo, Brazil

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Clinical and parasitological evaluation of dogs naturally infected by Leishmania (Leishmania) chagasi submitted to treatment with meglumine antimoniate and allopurinol

Aiming to assess the efficacy of the treatment, to verify the occurrence of possible disease relapses and to search for the presence of parasites after the treatment, seven dogs naturally infected by Leishmania sp., were submitted to a treatment with meglumine antimoniate and allopurinol. For this, lymph node and bone marrow aspiration biopsies were carried out at seven moments. After the end of the six-month observation period all dogs were submitted to euthanasia. Then, spleen and liver ���imprints��� and in vitro cultures were carried out to search for amastigote forms of the parasite. All animals presented remission of the symptoms and during all the observation period no dog presented relapse of the disease, although amastigote forms of the parasite were observed in two of the animals at the end of the experiment. Thus, it was possible to conclude that the treatment promotes clinical healing but it does not eliminate the parasites completely.

Use of crude, FML and rK39 antigens in ELISA to detect anti-Leishmania spp. antibodies in Felis catus

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

N��veis de IgG anti-Leishmania e perfil de citocinas em c��es machos e f��meas assintom��ticos naturalmente infectados por Leishmania (L.) Chagasi

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Anti-leishmania antibodies in cerebrospinal fluid from dogs with visceral leishmaniasis

Visceral leishmaniasis in Brazil is caused by Leishmania (Leishmania) chagasi and the dog is its most important reservoir. The clinical features in dogs include loss of weight, lymphadenopathy, renal failure, skin lesions, fever, hypergammaglobulinemia, hepatosplenomegaly, anemia, and, rarely, neurological symptoms. Most infected animals develop active disease, characterized by high anti-leishmania antibody titers and depressed lymphoproliferative ability. Antibody production is not primarily important for protection but might be involved in the pathogenesis of tissue lesions. An ELISA test was used to determine if there is an association between neurological symptoms and the presence of anti-L. chagasi antibodies in cerebrospinal fluid (CSF). Thirty serum and CSF samples from symptomatic mixed breed dogs (three with neurological symptoms) from a region of high incidence of visceral leishmaniasis in Brazil were examined for antibody using total parasite antigen and anti-dog IgG peroxidase conjugate. A high level of L. chagasi antibodies was observed in sera (mean absorbance �� SD, 1.939 �� 0.405; negative control, N = 20, 0.154 �� 0.074) and CSF (1.571 �� 0.532; negative control, N = 10, 0.0195 �� 0.040) from all animals studied. This observation suggests that L. chagasi can cause breakdown of filtration barriers and the transfer of antibodies and antigens from the blood to the CSF compartment. No correlation was observed between antibody titer in CSF and neurological symptoms.

Din��mica populacional de minic��rculos de cinetoplastos em Leishmania infantum chagasi

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)