Histopatologia e imunoistoquímica na distrofia muscular do Golden Retriever

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Unravelling the Neospora caninum secretome through the secreted fraction (ESA) and quantification of the discharged tachyzoite using high-resolution mass spectrometry-based proteomics

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Possible interspecific origin of the B chromosome of Hypsiboas albopunctatus (Spix, 1824) (Anura, Hylidae), revealed by microdissection, chromosome painting, and reverse hybridisation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Detecção e restauração de áreas sombreadas em imagens digitais de alta resolução espacial

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação da apoptose em tecido ovariano em gatas

Há um crescente interesse nas técnicas reprodutivas, por agilizar a procriação de populações animais de interesse econômico, a preservação de espécies em extinção ou a utilização como modelo experimental em outras espécies. A reprodução de felinos em cativeiro é dificultada devido ao ambiente estranho, o que os leva a apresentarem alterações na própria fisiologia. Torna-se importante o estudo de fenômenos reprodutivos destes animais com a finalidade de melhor compreensão e, posteriormente, adaptação adequada das biotécnicas reprodutivas. A atuação da apoptose na fisiologia ovariana ainda não é bem compreendida, havendo a necessidade da comunidade científica elucidá-la melhor. O objetivo deste trabalho foi testar a hipótese de que os índices de apoptose no tecido ovariano são diferentes em gatas jovens, adultas e idosas. Foram utilizadas 18 gatas distribuídas em 3 grupos contendo 6 animais cada, de acordo com a idade (jovens, adultos e idosos). As amostras do tecido ovariano destinadas a avaliação de apoptose, foram submetidas ao método terminal deoxynucleotidyltransferase-biotin nick end-labelling (TUNEL). Os dados referentes aos diferentes grupos foram submetidos à análise de variância e as médias foram comparadas pelo Teste de Tukey. Não houve diferença significativa quanto ao número de folículos e número de células positivas das gatas jovens, adultas e idosas, sendo P < 0,05. O resultado do trabalho sugere que o fenômeno da apoptose em tecido ovariano em gatas não possui relação com a faixa etária, ou seja, a apoptose ocorre de forma contínua nos folículos não-dominantes envolvidos em cada ciclo estral apresentado

Locating control points in aerial images with a multi-scale approach based on terrestrial image patches

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influence of the Paracoccidioides brasiliensis 14-3-3 and gp43 proteins on the induction of apoptosis in A549 epithelial cells

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Immunomodulatory Effects of Galectin-1 on an IgE-Mediated Allergic Conjunctivitis Model

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Characterization of a monoclonal antibody recognizing mast cells

Immunohistochemical screening for monoclonal antibodies prepared by immunization of mice with a rat osteoblastic cell population led to identification of one antibody that reacted against a small population of cells present in the soft connective tissue compartment of 21 days fetal rat calvaria. The morphology of the cells and the immunohistochemical staining characteristics (a distinct intracellular granular pattern) suggested that the antibody might be reacting specifically against mast cells. We used combined histochemistry and immunohistochemistry to further characterize this antibody, designated RCJ102. Cryosections containing calvaria bone, soft connective tissues and skin were prepared from the top of the head of 21 days fetal rats, and from adult rats cryosections of lung, muscle, adipose tissue and small intestine were prepared. Some sections were labelled by indirect immunofluorescence with RCJ102; corresponding sections were labelled histochemically with toluidine blue. There was a direct correspondence between mast cells identified histochemically and cells labelling with RCJ102 in all tissues except intestine, in which the mast cell detectable by histochemistry were not labelled by RCJ102. These results suggest that the RCJ102 antibody will be a valuable new reagent for further elucidation of the heterogeneity described between connective tissue and intestinal mucosal mast cells.

Study on the origin and nature of the adenomatoid odontogenic tumor by immunohistochemistry

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Apoptose no carcinoma de próstata

Pós-graduação em Patologia - FMB

Avaliação nutricional e conformidade da rotulagem de “petiscos” consumidos por crianças à legislação brasileira

Label is every and any information regarding to a product that is transcribed in its package. For the consumer it is through the nutritional information tables contained in the labels that there is access to data such as quantity and percentage of nutrients contained in foods. Through this knowledge, it is possible to make healthier food choices, minimizing the negative effects related to poor nutrition in the population, especially among children, the most critical rate of obesity incidence. The aim of this study was to evaluate the appropriateness of the labels of some foods consumed by children in relation to the Recommended Daily Intake (RDI) and verify that the general aspects of the labels were in accordance with Brazilian regulations. Five products were selected like snacks, corn snacks, snacks, peanut, peanuts, potato snacks and biscuit recipes. The labels of different brands of each snack were analyzed using the Checklist of Labelling, which is based on RDC No. 259 and RDC No. 360. The nutritional adequacy of nutrients from these foods (carbohydrates, protein, total fat, saturated fat, trans fat, dietary fiber and sodium) was evaluated in relation to that recommended by the RDA for children 4-8 years old. There was small percentage of mistakes in the labels of the analyzed foods, about 12%, being the presence of words that induce the consumers to the misunderstanding the irregularity with larger predominance, present in 25% of the labels. Other items in disagreement were the incomplete specification of the addictive ones alimentary in the list of ingredients and the absence of instructions about the conservation of the foods after opening the packings, both with percentile of occurrence of 18,75%. The high sodium content found in the nutritional information of food shows that should reduce the consumption of these products among children.

Avaliação comparativa do potencial osteocondutor de quatro diferentes substitutos ósseos em defeitos críticos em calvárias de coelhos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)