Hydrochemical evaluation of different spring waters for bottling

This investigation was carried out within the Parana sedimentary basin in São Paulo and Mato Grosso do Sul States, Brazil, and involved the sampling of different spring water samples with the purpose of evaluating their quality for bottling Several methods were utilized for acquiring the hydrochemical data, such as methyl orange end-point titration, potentiometry, ion selective electrodes, spectrophotometry, atomic absorption spectrophotometry and inductively coupled plasma spectrometry The results obtained for the analyzed samples were compared with the guideline values established by the São Paulo State and Brazilian Health Ministry legislations for defining the potable water standards The Brazilian Code for Mineral Waters that was established by Register 7841 published on 8 August 1945 was also taken into account for verifying if the spring waters are mineralized The hydrochemical data allowed the identification of anthropogenic and geogenic inputs of some constituents in most of the samples, which affected the water quality and did not allow them to be utilized for commercial purposes (bottling) The waters of these springs can only become appropriate for human consumption after previous chemical treatment

Seasonal variation of Alphitobius diaperinus population in broiler facilities in the center-north region of the state of São Paulo

The seasonality of Alphitobius diaperinus population was studied during different seasons during one year, in broiler facilities located in the center-north region of the State of São Paulo. Parasite counting was weekly carried out by the use of traps adapted from the modified model of Arends. During the experimental period, facilities were not submitted to chemical treatment, and the litter was changed between flocks. A lower number of adults and larvae was observed during autumn, with the peak registered in the summer. There was no significant difference between spring and winter averages (adults and larvae), and between spring and summer averages (adults). Average temperatures in the facilities varied from 23.4ºC (winter) to 27.1ºC (spring). The environmentally controlled systems used by the broiler industry provide an adequate habitat for A. diaperinus development. The continuous monitoring of the population of this parasite is essential for designing control strategies, and this can be accomplished by utilizing the traps used in the present experiment.

Qualidade fisiológica de sementes de soja tratadas com fungicidas durante e após o armazenamento

O presente trabalho teve o objetivo de avaliar o efeito do tratamento fungicida durante e após a armazenagem, na qualidade fisiológica de sementes de soja. Utilizaram-se sementes da cultivar BR-16, produzidas na safra de 1999/2000 e armazenadas em Marechal Cândido Rondon - PR. Para o tratamento utilizou-se a mistura de carbendazin + thiram, na dosage de 30 e 70g de i.a./100kg de semente, respectivamente. Realizou-se o tratamento de amostras mensalmente, de maio a dezembro de 2000, sendo as sementes analisadas imediatamente após o procedimento. Sementes sem tratamento representaram a testemunha. As amostras tratadas e armazenadas foram analisadas e comparadas com as tratadas e analisadas imediatamente antes da semeadura (dezembro de 2000). A qualidade das sementes foi avaliada através dos testes de germinação, de tetrazólio para vigor e viabilidade e emergência em campo. O delineamento experimental foi inteiramente casualizado, com quatro repetições por tratamento para cada teste. Realizou-se a comparação das médias através do teste de Tukey a 5% de probabilidade. O teste de germinação e o de tetrazólio não evidenciaram efeito negativo do tratamento fungicida anterior ao período de armazenagem sobre a qualidade das sementes. As sementes tratadas e não apresentaram decréscimo de viabilidade e de vigor, atingindo valores de germinação inferiores a 75%, em dezembro de 2000. Na emergência em campo, constatou-se que as sementes mantidas tratadas por mais de quatro meses apresentaram desempenho inferior comparativamente às tratadas nas demais épocas.

TRATAMENTO DE SEMENTES DE ARROZ, TRIGO, FEIJÃO E SOJA COM UM PRODUTO FORMULADO À BASE DE CÉLULAS E DE METABÓLITOS DE Bacillus subtilis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação de fontes de amônia para o tratamento de fenos de gramíneas tropicais. 1. Constituintes da parede celular, poder tampão e atividade ureática

Avaliaram-se as alterações da fração fibrosa e as características químicas dos fenos de braquiária decumbens (Brachiaria decumbnes Stapf) e jaraguá (Hyparrhenia rufa Ness Stapf) não-tratados, tratados com uréia (U-5,4% da MS), uréia (UL-5,4% da MS) mais labe-labe (Lablab purpureus L. Sweet, cv. Highworth-3,0% da MS) ou amônia anidra (NH3 -3,0% da MS). O tratamento químico com uréia ou NH3 aumentou o pH e a digestibilidade in vitro verdadeira dos fenos. A amonização não alterou os teores de fibra em detergente ácido e celulose, mas diminuiu os de fibra em detergente neutro, hemicelulose e lignina. O uso do labe-labe como fonte adicional de urease não aumentou a eficiência da uréia no tratamento dos volumosos. As avaliações do conteúdo de umidade, do poder tampão e da atividade ureática são técnicas que podem auxiliar na previsão das respostas dos volumosos à amonização com o uso de uréia.

Avaliação de fontes de amônia para o tratamento de fenos de gramíneas tropicais. 2. Compostos nitrogenados

Este estudo foi desenvolvido para se avaliarem as alterações nos conteúdos de compostos nitrogenados dos fenos de braquiária decumbens (Brachiaria decumbnes Stapf) e jaraguá (Hyparrhenia rufa Ness Stapf) não-tratados, tratados com uréia (U - 5,4% da MS), uréia (UL - 5,4% da MS) mais labe-labe (Lablab purpureus L. Sweet, cv. Highworth-3,0% da MS) ou amônia anidra (NH3 -3,0% da MS). A aplicação de amônia anidra ou de uréia aumentou os teores de N total, N insolúvel em detergente neutro, N insolúvel em detergente ácido, N não-protéico e N amoniacal. A amonização diminuiu as relações N insolúvel em detergente neutro/N total e N insolúvel em detergente ácido/N total e aumentou as relações N não-protéico/N total, N amoniacal/N total e os teores de PB. O N aplicado foi retido, principalmente, nas formas de NNP e N amoniacal.

Efeitos da amonização sobre a ocorrência de fungos, composição química e digestibilidade in vitro de fenos de grama Seda (Cynodon dactylon (L.) Pers.)

The experiment was conducted with grama seda (Cynodon dactylon (L.) Pers.) hays stored with a low moisture content (12-15%) and without chemical treatment, and hays stored with a high moisture content (20-25%) and treated with anhydrous ammonia (NH3) al 0.5 and 1.0% of DM, and urea at 0.9 and 1.8% of DM. At 65 days after treatment (AT) under a plastic cover, the bales were opened and samples were taken at 3, 15 and 30 days to determine the chemical composition and in vitro digestibility (IVDMD) of the hays. For the identification of fungi, samples were taken at 0, 15 and 30 days AT. The data were analyzed according to a split-plot design with the effects of the chemical treatments studied in the main plot and the effects of the periods of post-treatment studied in the sub-plots, Fourteen genera of fungi were observed in the hays, not treated and treated with NH3 and urea, with a higher occurrence of Cladosporium, Curvularia, Aspergillus, and Penicillium. Treatment with anhydrous ammonia and 1.8% urea controlled the occurrence of Aspergillus; however, Penicillium decreased in hays treated with ammonia 30 days AT. Ammoniation did not influence the contents of ADF, cellulose and lignin in the hays, but NDF and hemicellulose decreased with the use of ammonia 30 days AT. The CP contents and the IVDMD increased with ammoniation. The CP contents decreased in hays treated with NH3 as days AT increase, while hays treated with urea did not change.

Prediction of cassava starch edible film properties by chemometric analysis of infrared spectra

Cassava starch has been shown to make transparent and colorless flexible films without any previous chemical treatment. The functional properties of edible films are influenced by starch properties, including chain conformation, molecular bonding, crystallinity, and water content. Fourier-transform infrared (FTIR) spectroscopy in combination with attenuated total reflectance (ATR) has been applied for the elucidation of the structure and conformation of carbohydrates. This technique associated with chemometric data processing could indicate the relationship between the structural parameters and the functional properties of cassava starch-based edible films. Successful prediction of the functional properties values of the starch-based films was achieved by partial least squares regression data. The results showed that presence of the hydroxyl group on carbon 6 of the cyclic part of glucose is directly correlated with the functional properties of cassava starch films.

Mantispa minuta Fabricius (Neuroptera : Mantispidae) on egg sacs of the orb-weaver spider Parawixia bistriata (Araneidae) in forests of Eucalyptus grandis in Brazil

This is the first report of the interaction between the two entomophagous agents Mantispa minuta Fabricius (Mantispidae) and Parawixia bistriata Rengger, an orb-weaver spider (Araneidae). From egg sacs kept in the laboratory we observed the emergence of adult neuropterans followed by the emergence of a high number of spiderlings. This association illustrates the interactions among the invertebrate predators coexisting in Eucalyptus plantations managed without chemical treatment, in the State of São Paulo, Brazil.

Small angle X-ray scattering study of structural changes in silica gel modified with organofunctional groups

Silica gel surfaces, organofunctionalized with 2-mercaptobenzimidazole, iminosalicylaldehyde and imidazole groups were examined using the small angle X-ray scattering technique (SAXS). From the scattering intensity data it was concluded that particles have a uniform size after the coupling reaction. The chemical treatment of the silica gel leads to an attachment of the organofunctional groups on the solid-pore interface of the silica with an increase of the mean size of the solid phase and some coalescence of the pores. © 1989.

Clonal chromosome abnormalities found in three non-neoplastic proliferative brain lesions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Immediate replantation of maxillary incisors in rats: effects of tooth immersion in sodium fluoride and subsequent removal of the periodontal ligament.

The advantages and disadvantages of maintaining the periodontal ligament (PDL) in immediate replantation as well as chemical treatment of the root surface have been a matter of discussion because the vitality of such tissue in surgery is always questioned. This study evaluated the effects of conserving the tooth in sodium fluoride and the removal of the PDL before replantation of incisors in rats. There was more cementum-dentin resorption in the group with the PDL. The group without the PDL showed more discreet resorption, repair occurred through the newly formed bone tissue in the PDL space and ankylosis was more extensive than in the group with the PDL.

Efeito do Tratamento com Hidróxido de Sódio sobre a Fração Fibrosa, Digestibilidade e Tanino do Feno de Jurema-Preta (Mimosa tenuiflora. Wild)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA.

A study was conducted to evaluate in vitro the effect of root surface conditioning with basic fibroblast growth factor (b-FGF) on morphology and proliferation of fibroblasts. Three experimental groups were used: non-treated, and treated with 50 microg or 125 microg b-FGF/ml. The dentin samples in each group were divided into subgroups according to the chemical treatment received before application of b-FGF: none, or conditioned with tetracycline-HCl or EDTA. After contact with b-FGF for 5 min, the samples were incubated for 24 h with 1 ml of culture medium containing 1 x 10(5) cells/ml plus 1 ml of culture medium alone. The samples were then subjected to routine preparation for SEM, and random fields were photographed. Three calibrated and blind examiners performed the assessment of morphology and density according to two index systems. Classification and regression trees indicated that the root surfaces treated with 125 microg b-FGF and previously conditioned with tetracycline-HCl or EDTA presented a morphology more suggestive of cellular adhesion and viability (P = 0.004). The density of fibroblasts on samples previously conditioned with EDTA, regardless of treatment with b-FGF, was significantly higher than in the other groups (P < 0.001). The present findings suggest that topical application of b-FGF has a positive influence on both the density and morphology of fibroblasts.

Efeito do tratamento químico na sanidade de sementes de espécies florestais

This study evaluated the effect of different fungicide and sodium hypochlorite treatments in reducing the incidence of fungi on seeds of native species of the Atlantic rainforest. The experiment was done using the completely randomized design in a 5×5 factorial with five levels of factor A (forest species) and five levels of factor B (chemical treatment and surface disinfection with sodium hypochlorite) with four replications. The fungal genera Fusarium, Alternaria, Aspergillus and Penicillium were identified. The species 'Jacarandáda- Bahia' and 'Ipê-roxo' had lower fungal incidence, 1.0% and 1.3%, respectively. Except for 'Angico Vermelho', the fungicide pencycurom and sodium hypochlorite were not significantly different from the control in the species evaluated. Seed treatment with fungicides 'Captan' and 'Tiram' yielded satisfactory results in reducing the incidence of fungal contamination in forest species seeds.