Imputation of microsatellite alleles from dense SNP genotypes for parentage verification across multiple Bos taurus and Bos indicus breeds

To assist cattle producers transition from microsatellite (MS) to single nucleotide polymorphism (SNP) genotyping for parental verification we previously devised an effective and inexpensive method to impute MS alleles from SNP haplotypes. While the reported method was verified with only a limited data set (N = 479) from Brown Swiss, Guernsey, Holstein, and Jersey cattle, some of the MS-SNP haplotype associations were concordant across these phylogenetically diverse breeds. This implied that some haplotypes predate modern breed formation and remain in strong linkage disequilibrium. To expand the utility of MS allele imputation across breeds, MS and SNP data from more than 8000 animals representing 39 breeds (Bos taurus and B. indicus) were used to predict 9410 SNP haplotypes, incorporating an average of 73 SNPs per haplotype, for which alleles from 12 MS markers could be accurately be imputed. Approximately 25% of the MS-SNP haplotypes were present in multiple breeds (N = 2 to 36 breeds). These shared haplotypes allowed for MS imputation in breeds that were not represented in the reference population with only a small increase in Mendelian inheritance inconsistancies. Our reported reference haplotypes can be used for any cattle breed and the reported methods can be applied to any species to aid the transition from MS to SNP genetic markers. While ~91% of the animals with imputed alleles for 12 MS markers had ≤1 Mendelian inheritance conflicts with their parents' reported MS genotypes, this figure was 96% for our reference animals, indicating potential errors in the reported MS genotypes. The workflow we suggest autocorrects for genotyping errors and rare haplotypes, by MS genotyping animals whose imputed MS alleles fail parentage verification, and then incorporating those animals into the reference dataset.

Publicações da revista odontológica de Araçatuba e sua importância na área odontológica

The Araçatuba Dental Journal was created with the purpose of disseminating scientific papers produced in universities of São Paulo state and from other localizations in Brazil. The aim of this study was to analyze and quantify the papers published in the Araçatuba Dental Journal from 2001 to 2011. Twenty two online editions were studied. A higher number of scientific studies (44,54%) was found compared to literature reviews (27,73%) and case reports (27,73%). The Araçatuba Dental School (UNESP) was the instituition that most published papers in the journal (44,12%). It can be concluded that regardless the analyzation process, the mentioned journal provides to the readers the opportunity to update their knowledge in dentistry.

Como realizar o selamento periférico e a moldagem funcional?

The functional impression technique is one of the most important steps for complete and/or partial edentulism treatment. So, correct analysis and record of supporting structures are essential to determine the denture extension. The functional impression technique has two stages - a customized final impression tray and a border impression - which aims to record the optimal denture supporting area. The aim of this study was to review the literature related to border impression and functional impression techniques, describing the technique and materials, and identifying the procedure’s objectives and function.

Purificação parcial e caracterização bioquímica de uma isoforma de β-glicosidase do fungo termofílico Myceliophthora thermophila M.7.7

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)