Efeitos da pressão crítica (25 cm H2O) e mínima de selo do balonete de tubos traqueais sobre a mucosa traqueal do cão

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Intravenous versus inhalation anaesthesia for one-lung ventilation

BackgroundThis is an update of a Cochrane Review first published in The Cochrane Library, Issue 2, 2008.The technique called one-lung ventilation can confine bleeding or infection to one lung, prevent rupture of a lung cyst or, more commonly, facilitate surgical exposure of the unventilated lung. During one-lung ventilation, anaesthesia is maintained either by delivering an inhalation anaesthetic to the ventilated lung or by infusing an intravenous anaesthetic. It is possible that the method chosen to maintain anaesthesia may affect patient outcomes. Inhalation anaesthetics may impair hypoxic pulmonary vasoconstriction (HPV) and increase intrapulmonary shunt and hypoxaemia.ObjectivesThe objective of this review was to evaluate the effectiveness and safety of intravenous versus inhalation anaesthesia for one-lung ventilation.Search methodsWe searched the Cochrane Central Register of Controlled Trials (CENTRAL); The Cochrane Library (2012, Issue 11); MEDLINE (1966 to November 2012); EMBASE (1980 to November 2012); Literatura Latino-Americana e do Caribe em Ciencias da Saude (LILACS, 1982 to November 2012) and ISI web of Science (1945 to November 2012), reference lists of identified trials and bibliographies of published reviews. We also contacted researchers in the field. No language restrictions were applied. The date of the most recent search was 19 November 2012. The original search was performed in June 2006.Selection criteriaWe included randomized controlled trials and quasi-randomized controlled trials of intravenous (e. g. propofol) versus inhalation (e. g. isoflurane, sevoflurane, desflurane) anaesthesia for one-lung ventilation in both surgical and intensive care participants. We excluded studies of participants who had only one lung (i.e. pneumonectomy or congenital absence of one lung).Data collection and analysisTwo review authors independently assessed trial quality and extracted data. We contacted study authors for additional information.Main resultsWe included in this updated review 20 studies that enrolled 850 participants, all of which assessed surgical participants no studies investigated one-lung ventilation performed outside the operating theatre. No evidence indicated that the drug used to maintain anaesthesia during one-lung ventilation affected participant outcomes. The methodological quality of the included studies was difficult to assess as it was reported poorly, so the predominant classification of bias was 'unclear'.Authors' conclusionsVery little evidence from randomized controlled trials suggests differences in participant outcomes with anaesthesia maintained by intravenous versus inhalational anaesthesia during one-lung ventilation. If researchers believe that the type of drug used to maintain anaesthesia during one-lung ventilation is important, they should design randomized controlled trials with appropriate participant outcomes, rather than report temporary fluctuations in physiological variables.

Dose inseminante para fertilização artificial de ovócitos de dourado

Objetivou-se determinar a dose inseminante adequada para uso na fertilização artificial de ovócitos de dourado (Salminus brasiliensis). Os ovócitos foram distribuídos em delineamento inteiramente casualizado, e fertilizados com uma das relações espermatozoides/ovócito 6,0×10³; 6,0×10(4); 6,0×10(5); 6,0×10(6) ou 3,0×10(7), cada uma com quatro repetições. Considerou-se unidade experimental uma incubadora de volume útil de 2,5 L, contendo 2,0 mL de ovócitos não-hidratados. As taxas de fertilização foram mensuradas 8 horas após o início da fertilização. Com intuito de verificar possíveis efeitos da diluição seminal na movimentação dos espermatozoides, realizou-se a mensuração do tempo de duração da motilidade espermática dos espermatozoides de dourado, ativados por meio de diferentes relações de diluição: 6,8×10-5; 6,8×10-4; 6,8×10-3; 6,8×10-2; 3,4×10-1 e 1,0 mL de sêmen por mL de água. O tempo de duração da motilidade foi avaliado em delineamento inteiramente casualizado composto de seis tratamentos e três repetições. As taxas de fertilização apresentaram relação quadrática com o número de espermatozoides por ovócito. As relações de diluição do sêmen tiveram efeito inversamente proporcional sobre a duração da motilidade espermática. A relação que proporcionou melhores taxas de fertilização artificial de ovócitos de dourado (Salminus brasiliensis) foi de 30.722 espermatozoides por ovócio.

Artificial fertilization of oocytes and sperm activation in pacu: effects of the spermatozoa:oocyte ratio, water volume, and in natura semen preservation

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effects of the spermatozoa: oocyte ratio, water volume and water temperature on artificial fertilization and sperm activation of cascudo-preto

The objective of this study was to evaluate the effects of water volume and water temperature on the sperm motility duration and the number of spermatozoa, and the water volume on the fertilization rates of oocytes of Rhinelepis aspera. Experiments were carried out to evaluate the effect of semen dilutions (1.74×10-5, 1.74×10-4, 1.74×10-3, 1.74×10-2, 1.74×10-1 and 1.00 mL of sperm.mL-1 of water) and water temperature (5, 10, 15, 20, 25, 30, 35, 40, 45, and 50 ºC) on spermatozoa motility duration. In addition, the effects of insemination dose (7×10³, 7×10(4), 7×10(5), 7×10(6) and 7×10(7) spermatozoa.oocyte-1) and water volume (1.0, 30.0, 60.0, 90.0 and 120.0 mL water.2.0 mL-1 oocytes) on the artificial fertilization rates of oocytes were evaluated. The longest sperm motility duration were observed for the semen dilution of 1.74×10-5 mL semen.mL-1 water and in water at 5 ºC. The highest fertilization rates were obtained for insemination doses between 7.00×10³ and 1.23×10(7) spermatozoa. oocyte-1 and water volume of 28.11 mL water.2.0 mL-1 oocytes.

Air velocity and exposure time to ventilation affect body surface and rectal temperature of broiler chickens

Increasing air movement over poultry by using fans (ventilation) has become an accepted means of reducing environmental heat stress over the last several years. The purpose of this study was to evaluate the effect of air velocity and exposure time to ventilation on body surface and rectal temperature of broiler chickens. Male broiler chickens aged 36-42 days were placed in individual wire cages and exposed to five different air velocities (5.7, 4.2, 3.1, 2.4, or 1.8 m/sec). Throughout the experiment head, back, leg, and rectal temperatures were monitored every 10 min during a 30-min period for each air velocity. The data showed that exposure time to the wind affected (P<.05) leg and body temperature, with a rapid reduction being observed during the first 10 min. There was a reduction in leg temperature with air velocity of 2 m/sec; however, air velocity lower than 4.5 m/sec was not effective in decreasing head and back temperature. The results suggest that air velocity of 2 m/sec, in air temperature of 29 degrees C, improves heat loss in the birds. The data also indicate that exposure time to ventilation seems to be a critical point in the maintenance of bird thermal homeostasis.

Florescimento e frutificação do maracujazeiro-amarelo submetido à iluminação artificial, irrigação e sombreamento

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Caracterização físico-química de frutos de maracujazeiro-amarelo submetidos à iluminação artificial, irrigação e sombreamento

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Fertility Rates Following Fixed-Time Artificial Insemination in Dairy Heifers in a Practical Progesterone-Based Protocol

Background: In bovines, more efficient management practices are important for maximizing profitability. In order to increase the pregnancy rates in artificial insemination (AI) programs, several hormonal protocols were developed to synchronize the follicular wave and the moment of ovulation in beef and dairy cattle. In dairy cattle, detection of estrus can be difficult due to a number of factors including the incidence of silent estrus. Hormonal treatments designed to control both luteal and follicular function has permitting efficient synchronizations of time of ovulation. Thus, the AI can be performed in a large number of animals on a fixed schedule without the need for detection of estrus. Using these management techniques, the fixed-time artificial insemination (TAI) can overcome the problem of accurate estrus detection and help in reducing the incidence of repeat breeding. In addition, with TAI in cattle operations, it is possible to facilitate management practices and commercialization, and to reduce the time and semen wasting with animals inseminated at incorrect times. The investigation of practical and efficient TAI protocols is important for reducing the labor and animal handling of TAI in dairy cattle, as well as for increasing the profitability of the cattle management system. This study was carried out in order to investigate the effectiveness of TAI in dairy heifers treated with a practical progesterone-based protocol.Materials, Methods & Results: This experiment was conducted at the university farm located in southwestern Brazil, during May 2009. Thirty-nine cycling crossbred dairy heifers were employed in this study. All animals received a single intramuscular injection of estradiol benzoate and intravaginal progesterone releasing device in a random stage of the estrous cycle (Day 0). on day 7 the animals were treated with PGF2a analogue and on day 9 the device was removed. Forty-eight hours after the device removal (day 11) a synthetic analogue of GnRH was administered and the animals were fixed-time artificially inseminated at the time of GnRH injection. The inseminations were performed using four different batches from the same Holstein bull. Among the heifers that were synchronized (87.2%), 30.8% ovulated until 24 h after TAI and 56.4% ovulated between 24 and 32 h after TAI. The conception rate was 61.5%. No effects of ovulation time in conception rates were detected. The conception rate from heifers that ovulated until 24 h after TAI was 58.3% and from heifers that ovulated between 24 and 32 h after TAI was 77.3%. The mean of ovulatory follicle in heifers that ovulated until 24 h was 14.3 mm and in heifers that ovulated between 24 and 32 h was 11.9 mm.Discussion: Taking together, the findings of the present study, along with those of others, emphasize the concept that development of practical methods for TAI offers significant advantages to dairy producers if conception rates are close or greater to those obtained after breeding at detected estrus. Thus, the results of the present study reinforce the possibility of making dairy cattle production more cost-effective using TAI. In conclusion, with the progesterone-based TAI protocol of the present experiment all synchronized animals ovulated up to 32 h after GnRH+TAI and no effects of ovulation time related to conception rate was detected. The exogenous control of luteal and follicular development facilitated the reproductive management and animal handling. Also, inseminating the heifers at the moment of GnRH injection in a progesterone-based TAI protocol is a practical strategy and provided satisfactory results regarding ovulation and conception rates in dairy heifers.

Crescimento, partição de fotoassimilados e produção de rizomas em taro cultivado sob sombreamento artificial

O cultivo associado é uma alternativa para culturas de ciclo longo e propriedades com limitação de área. Todavia, para o estabelecimento de associações, há necessidade de conhecer a tolerância das espécies ao sombreamento. O trabalho objetivou avaliar o crescimento de parte aérea, partição de fotoassimilados e produção de rizomas em plantas de taro 'Japonês' cultivadas sob intensidades e períodos de sombreamento artificial. Utilizou-se o delineamento de blocos ao acaso, com 13 tratamentos e quatro repetições. Os tratamentos foram constituídos de quatro intensidades de sombreamento (controle = pleno sol; 18; 30 e 50% de sombra, mantidos durante o ciclo todo), além da implementação das intensidades de sombra de 18; 30 e 50%, em três períodos (inicial = 0 a 3; intermediário = 3 a 6 e final = 6 a 9 meses). Aos 60; 90; 120; 150; 180; 210; 240 e 270 dias após o plantio (dat), avaliou-se crescimento de planta e partição de massa entre órgãos e aos 270 dat a produção de rizomas. Plantas sob sombreamento, sobretudo nas maiores intensidades e durante o ciclo todo, apresentaram maior produção de biomassa de parte aérea e de rizomas-mãe e filhos pequenos, em detrimento de rizomas-filho grandes, médios e comerciáveis. A intensidade de 18% de sombra, durante todo o ciclo ou nos períodos inicial e intermediário, foi a que menos afetou o desenvolvimento das plantas e produção de biomassa de rizomas-filho comerciáveis.

Comportamento de chamamento de Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) em Psidium guajava, Eucalyptus grandis e em dieta artificial

Este estudo teve como objetivos determinar e comparar o efeito de três dietas: Psidium guajava, Eucalyptus grandis e dieta artificial no comportamento de chamamento e no padrão temporal do comportamento de chamamento de fêmeas virgens de Thyrinteina arnobia (Stoll). Foram analisados os parâmetros: tempo médio para o início do chamamento, número médio de chamamento, tempo médio de cada chamamento e tempo médio total de chamamento. Os bioensaios relacionados ao comportamento de chamamento de fêmeas virgens foram realizados durante seis escotofases consecutivas e as observações tomadas a cada 5 min., a 22 ± 1ºC, 70 ± 5% de UR e 10h de escotofase. As fêmeas, independente da dieta analisada, apresentaram duas posições diferentes de chamamento. A maioria das fêmeas virgens iniciou o chamamento na 1ª hora da 1ª escotofase. O padrão de chamamento da fêmea individualizada foi característico de um padrão contínuo. O tipo de dieta oferecida na fase larval influenciou o início do comportamento de chamamento (pré-chamamento) e o tempo médio total de chamamento. O tipo de dieta também alterou a coloração da glândula de feromônio.

Sites of Defoliation by Atta sexdens rubropilosa (Hymenoptera, Formicidae) in Artificial Plants

This study aimed to achieve a better understanding about the foraging behavior of leaf-cutter ant (Atta sexdens rubropilosa Forel) workers with respect to defoliation sites in plants. To accomplish that, artificial plants 70 cm in height were prepared and divided into four levels (heights), having natural plant leaves attached to them. Evaluations during the bioassays included the number of leaves dropped by the ants, as well as the percentage of plant mass removed. In all replicates, it became evident that the most exploited plant site is the apical region, which significantly differed from other plant levels.

Ventilação mecânica não-invasiva no pós-operatório de cirurgia cardíaca: atualização da literatura

Este estudo objetivou atualizar os conhecimentos em relação à utilização da ventilação mecânica não-invasiva (VMNI) no pós-operatório de cirurgia cardíaca e identificar se há indícios da superioridade de uma forma de modalidade de VMNI em relação à outra. Foi realizada revisão da literatura entre 2006 a 2011, a partir das bases de dados PubMed, SciELO e Lilacs, utilizando os descritores respiração artificial, pressão positiva contínua nas vias aéreas, ventilação com pressão positiva intermitente e cirurgia cardíaca, e seus correspondentes na língua inglesa, os quais foram pesquisados em cruzamentos. A partir dos critérios adotados, foram selecionados nove artigos, dos quais seis demonstraram aplicações de VMNI, por meio de modalidades como pressão positiva contínua nas vias aéreas, pressão positiva com dois níveis pressóricos e respiração com pressão positiva intermitente, no pós-operatório de cirurgia cardíaca, e, três realizaram comparações entre as diferentes modalidades. As modalidades de VMNI descritas na literatura foram utilizadas com resultados satisfatórios. Estudos que comparam diferentes modalidades são escassos, contudo alguns demonstraram superioridade de uma modalidade de VMNI, como é o caso da respiração com pressão positiva intermitente na reversão da hipoxemia e da pressão positiva com dois níveis pressóricos na melhora da oxigenação, da frequência respiratória e frequência cardíaca desses pacientes, em comparação a outras modalidades.

Influence of respiratory biofeedback associated to re-expansive ventilation patterns in individuals with functional mouth breathing

Objectives: Assess the effect of re-expansive respiratory patterns associated to respiratory biofeedback (RBF) on pulmonary function, respiratory muscle strength and habits in individuals with functional mouth breathing (FMB).Methods: Sixty children with FMB were divided into experimental and control groups. The experimental group was submitted to 15 sessions of re-expansive respiratory patterns associated to RBF (biofeedback pletsmovent; MICROHARD (R) V1.0), which provided biofeedback of the thoracic and abdominal movements. The control group was submitted to 15 sessions using biofeedback alone. Spirometry, maximum static respiratory pressure measurements and questions regarding habits (answered by parents/guardians) were carried out before and after therapy. The Student's t-test for paired data and non-parametric tests were employed for statistical analysis at a 5% Level of significance.Results: Significant changes were found in forced vital. capacity, Tiffeneau index scores, maximum expiratory pressure, maximum inspiratory pressure and habits assessed in FMB with the use of RBF associated to the re-expansive patterns. No significant differences were found comparing the experimental and control groups.Conclusions: The results allow the conclusion that RBF associated to re-expansive patterns improves forced vital capacity, Tiffeneau index scores, respiratory muscle strength and habits in FMB and can therefore be used as a form of therapy for such individuals. (C) 2008 Elsevier B.V. All rights reserved.