Characterization of peroxidase changes in tolerant and susceptible soybeans challenged by soybean aphid (Hemiptera: Aphididae)

Changes in protein content, peroxidase activity, and isozyme profiles in response to soybean aphid feeding were documented at V1 (fully developed leaves at unifoliate node, first trifoliate leaf unrolled) and V3 (fully developed leaf at second trifoliate node, third trifoliate leaf unrolled) stages of soybean aphid-tolerant (KS4202) and -susceptible (SD76R) soybeans. Protein content was similar between infested and control V1 and V3 stage plants for both KS4202 and SD76R at 6, 16, and 22 d after aphid introduction. Enzyme kinetics studies documented that control and aphid-infested KS4202 V1 stage and SD76R V1 and V3 stages had similar levels of peroxidase activity at the three time points evaluated. In contrast, KS4202 aphid-infested plants at the V3 stage had significantly higher peroxidase activity levels than control plants at 6 and 22 d after aphid introduction. The differences in peroxidase activity observed between infested and control V3 stage KS4202 plants at these two time points suggest that peroxidases may be playing multiple roles in the tolerant plant. Native gels stained for peroxidase were able to detect differences in the isozyme profiles of aphid-infested and control plants for both KS4202 and SD76R.

Níveis de sobrecarga, ansiedade e depressão de cuidadores de pacientes com doença de Alzheimer participantes e não participantes de grupos de apoio

Currently , as a result of the significant increase in the number of elderly, one can observe an increase in the number of chronic diseases , among them Alzheimer's disease (AD) , which affects both patients and their caregivers , that due care with the patient , just overwhelmed , anxious and depressed. Therefore, this study aims to draw a profile of caregivers of Alzheimer's patients, correlating the physical activity of patients with levels of overload, anxiety, depression caregivers. For both sample consisted of 40 patients with AD to characterize the physical activity levels and perform activities of daily living. In addition, we evaluated 40 caregivers. Patient assessment was made through a medical history, beyond the score Clinical Dementia Rating (CDR), Mini - Mental State Examination (MMSE), Modified Baecke Questionnaire for Older Adults (MBQ) , Functional Activities Questionnaire PFEFFER (QAFP) , and the Self Perception Performance in Activities of Daily Living (EAPAVD). For assessing the caregiver was also used an interview, then the Neuropsychiatric Inventory (NPI), anxiety and depression scale (HAD) Scale and the Zarit caregiver burden (Zarit). The data were processed using descriptive procedures for the analysis of characterization of samples, such as cognitive screening and physical activity level and profile of caregivers. There was a normal distribution of the data using the Shapiro Wilk, For data with normal distribution were used parametric descriptive procedures using One Way ANOVA to compare groups and applying a post hoc Bonferroni. As for the data that showed the destruction not normal was used to standardize the test by Z -score , and then treated by means of parametric statistical procedures , as presented earlier . The Pearson correlation was used to identify possible associations between variables. It was assumed significance level of 5 % (p ≤ 0.05) for all analyzes. Given these results, we conclude that...

Comparação da aptidão física em idosos submetidos a diferentes intervenções de exercício físico

Introduction: The elderly population has increased, and the aging process is responsible for physical and metabolic changes in the body. For the elderly remain independent, with a better quality of life, preventing and treating diseases responsible for this increased of mortality should remain physically active. Objective: To investigate the influence of two types of exercise intervention on physical fitness and physical activity levels in older practitioners of Rio Claro-SP. Method: The study included 18 older adults with mean age 65.8 years (± 2.88) divided into two groups, Program in Physical Exercises Health Unit (PEFUS, n = 9) and Adapted Volleyball (n = 9). Classes of PEFUS were held twice a week lasting 60 min with strength, aerobic endurance, agility, coordination and balance exercises. Classes of Volleyball Adapted were performed 2 times per week lasting 120 minutes, divided into volleyball fundamental exercises and game. For evaluation of physical skills (strength, agility, coordination and flexibility), was performed the AAHPERD test battery. All participants were assessed at the baseline and after 3 months of interventions. The statistical analysis used was the repeated measures ANOVA, through the SPSS version 17.0 and significance level p <0.05. Result: After the interventions there was an increase of leisure time from 362.2 ± 214.9 min / week and 16.7 ± 28.3 min / week to 541.7 ± 137.2 and 44.4 ± 44.8 min / week to Adapted Volleyball and PEFUS, respectively. The variable coordination showed significant improvements after the interventions, decreasing from 12.1 ± 0.7 to 10.8 ± 0.5 sec for Volleyball Adapted and from 14.8 ± 0.9 to 12.1 ± 0, 9 sec for PEFUS (p <0.05). For the variable strength it was observed a group-moment interaction (p <0.05). Conclusion: The interventions showed positive results... (Complete abstract click electronic access below)

Dinâmica linear e não linear da variabilidade da frequência cardíaca: sensibilidade, especificidade, valor preditivo e correlação de características físicas e clínicas no Diabetes Mellitus tipo 1

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Extrato hidroalcoólico de Punica granatum Linn. sobre Staphylococcus spp. isolado de leite bovino

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Chromosome mapping of retrotransposable elements Rex1 and Rex3 in Leporinus Spix, 1829 species (Characiformes: Anostomidae) and its relationships among heterochromatic segments and W sex chromosome

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Higher screen time is associated with overweight, poor dietary habits and physical inactivity in Brazilian adolescents, mainly among girls

To analyse the associations between high screen time and overweight, poor dietary habits and physical activity in adolescents according to sex. The study comprised 515 boys and 716 girls aged 14-17 years from Londrina, Brazil. Nutritional status (normal weight or overweight/obese) was assessed by calculating the body mass index. Eating habits and time spent in physical activity were reported using a questionnaire. The measurement of screen time considered the time spent watching television, using a computer and playing video games during a normal week. Associations between high screen time and dependent variables (nutritional status, eating habits and physical activity levels) were assessed by binary logistic regression, adjusted for sociodemographic and lifestyle variables. Most adolescents (93.8% of boys and 87.2% of girls) spent more than 2 hours per day in screen-time activities. After adjustments, an increasing trend in the prevalence of overweight and physical inactivity with increasing time spent on screen activities was observed for both sexes. Screen times of >4 hours/day compared with <2 hours/day were associated with physical inactivity, low consumption of vegetables and high consumption of sweets only in girls and the consumption of soft drinks in both sexes. The frequency of overweight and physical inactivity increased with increasing screen time in a trending manner and independently of the main confounders. The relationship between high screen time and poor eating habits was particularly relevant for adolescent girls.

Prática de atividades físicas, doenças crônicas não transmissíveis e mortalidade entre adultos usuários da atenção primária do sistema público de saúde de Bauru, São Paulo: estudo longitudinal

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Variáveis antropométricas e hemodinâmicas em relação a diferentes níveis e categorias de atividade física

This study aimed to investigate the relation between physical activity levels (PAL) of hypertensive patients, seeing different do- mains, and, to compare anthropometric variables and blood pressure (BP). The survey was conducted with 192 patients, mean age 63 ± 11 years. The anthropometric variables were body weight, body mass index (BMI) and waist circumference (WC). PAL was estimated using Baecke ́s questionnaire and categorized in four domains (OPA = occupational; leisure time = LTPA; locomotion = LPA and Total PA). Student t test unpaired was applied for comparisons between active and insufficiently active. Differences between active and sedentary in relation to anthropometry and resting blood pressure were observed for the LTPA, LPA and Total PA and significant negative correlations were observed between LPA and Total PA with systolic BP (p<0,05). Conclusion: the LPA and LTPA were associated with resting BP in hypertensive. No differences in BMI and WC among physically active and insuffi- ciently active were found.

Fatores associados aos componentes de aptidão e nível de atividade física de usuários da Estratégia de Saúde da Família, Município de Botucatu, Estado de São Paulo, Brasil, 2006 a 2007

Fundo de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Lipodystrophy in HIV/AIDS patients with different levels of physical activity while on antiretroviral therapy

INTRODUÇÃO: A lipodistrofia relacionada ao uso de terapia antirretroviral (TARV) pode causar estigma estético e elevar o risco de doenças cardiovasculares. A atividade física pode ser uma alternativa válida para o tratamento e prevenção da lipodistrofia. Entretanto, poucos estudos tratam dessa temática. O objetivo deste estudo foi verificar a ocorrência de lipodistrofia relacionada ao uso de TARV em portadores de HIV/AIDS, com diferentes hábitos de atividades físicas. MÉTODOS: A casuística foi formada por 42 portadores de HIV em uso de TARV, do Centro de Testagem e Aconselhamento de Presidente Prudente. Para obtenção do nível de atividade física aplicou-se o Questionário Internacional de Atividade Física (IPAQ); a lipodistrofia foi diagnosticada pelo autorrelato do paciente e a confirmação médica. O percentual de gordura de tronco foi estimado pela absortometria por raio-X de dupla energia (DEXA). Foram coletados também dados referentes a sexo, idade, tempo de uso de TARV, valores de CD4 e carga viral. RESULTADOS: Verificou-se maior ocorrência de lipodistrofia no grupo sedentário quando comparado ao ativo, além de fator protetor da prática da atividade física em relação à ocorrência da lipodistrofia. O grupo com valores mais elevados de CD4 também apresentou maior proporção de sujeitos com lipodistrofia, além de maior proporção de ativos e de indivíduos com menor faixa etária. Os acometidos pela lipodistrofia apresentaram maiores valores de percentual de gordura de tronco, bem como, os sedentários em relação aos ativos. CONCLUSÕES: O estilo de vida fisicamente ativa resultou em efeito protetor para ocorrência da lipodistrofia relacionada ao uso da TARV.

Effects of malathion and cadmium on acetylcholinesterase activity and metallothionein levels in the fish Seriola dumerilli

The potential use of acetylcholinesterase (AChE) and metallothionein (MT) responses as biomarker of organophosphorous (OPs) and trace metal were assessed in fish Seriola dumerilli exposed to 0, 4, 6 mg/kg of malathion for 2, 7 and 13 days, and to 0, 50, 100, 250 mu g/kg of Cd for 2 days. Brain AChE was significantly inhibited after 2 and 7 days of malathion exposure, in a dose-response manner, but no inhibition was observed after 13 days of exposure. When exposed to Cd for 2 days, S. dumerelli presented an increase in AChE activity at a concentration of 50 mu g/kg, but a strong and dose-dependent AChE inhibition at 100 and 250 mu g/kg. Cd treatment also caused a rapid increase in MTs concentration in liver, even at the lower concentration. Our experiments indicate that the measurement of hepatic MT concentration and brain AChE activity in S. dumerilli would be useful biomarkers of OP and Cd exposure and/or effects.

Effects of prenatal exposure to deltamethrin on forced swimming behavior, motor activity, and striatal dopamine levels in male and female rats

The effects of prenatal exposure of rat pups to 0.08 mg/kg deltamethrin (DTM) on physical, reflex and behavioral developmental parameters, on forced swimming and open-field behaviors, and on striatal monoamine levels at 60 days of age were observed. Maternal and offspring body weight, physical and reflex development were unaffected by the exposure to the pesticide. At 21 days of age, open-field locomotion frequency and immobility duration of male and female offspring were not different between control and exposed animals. However, male rearing frequency was increased in experimental animals. A decreased immobility latency to float and in general activity after the swimming test in male offspring was observed at adult age; no interference was detected in the float duration during the swimming test. In addition, these animals presented higher striatal 3,4-dihydroxyphenylacetic acid (DOPAC) levels without modification in dopamine (DA) levels and an increased DOPAC/DA ratio. These data indicate a higher activity of the dopaminergic system in these animals. Noradrenaline (NA) levels were increased, while MHPG levels were not detectable in the system studied. Serotonin (5-HT) and 5-hydroxyindolacetic acid (5-HIAA) levels, as well as the homovanillic acid (HVA)/DA ratio, were not modified by the exposure to the pesticide. No changes were observed in swimming and open-field behaviors nor were there any changes in striatal monoamines or their metabolites in the female experimental group. In relation to the pesticide formula, the present data showing that prenatal exposure to DTM alters latency to float and the activity of striatal dopaminergic system might reflect a persistent effect of the pesticide on animal motor activity, mainly in males. on the other hand, the decrease in general activity observed in experimental male rats suggests higher levels of emotionality induced by previous exposure to the swimming behavior test in relation to control animals. Data gathered in the present study may be important for the assessment of the safety of pyrethroid insecticides. (C) 2001 Elsevier B.V. All rights reserved.