Estudo do músculo diafragma em ratos com insuficiência cardíaca crônica: composição das miosinas e expressão dos fatores de regulação miogênica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização morfológica, bioquímica e molecular do músculo esquelético sóleo de ratos espontaneamente hipertensos com insuficiência cardíaca

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência da alimentação inicial no crescimento muscular e na expressão da MyoD e Miogenina em larvas de pacu

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Adaptações morfofuncionais e respostas moleculares do músculo esquelético de ratos submetidos ao treinamento resistido

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da temperatura sobre a expressão de fatores miogênicos no músculo estriado esquelético do pacu (Piaractus mesopotamicus)

Pós-graduação em Biologia Geral e Aplicada - IBB

Morphology and expression of genes related to skeletal muscle growth in juveniles of pirarucu (Arapaima gigas, Arapaimatidae, Teleostei)

Skeletal muscle growth in the pirarucu (Arapaima gigas) is highly interesting to fish farmers because it provides information about how the mechanism in muscle mass increase, characteristic of the species, is regulated. Pirarucu has specific muscle growth that highlights the species's significance and commercial value. Current research evaluates the morphology and the growth-related gene expression in the red and white skeletal muscles of the pirarucu. Muscle samples were collected from the lateral anterior region and frozen in liquid nitrogen. Histological sections were performed and stained by HE for morphological analysis. Red and white muscle samples were used to determine MyoD, myogenin, and myostatin genes expression by Real-time Polymerase Chain Reaction. Although MyoD and myogenin were not statistically different in the two types of muscles, myostatin was significantly higher in the white rather than in the red muscle. Results show the muscle growth characteristics of the species and may be helpful for improving aquaculture management programs.

Rearing temperature induces changes in muscle growth and gene expression in juvenile pacu (Piaractus mesopotamicus)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Heart Failure-Induced Diaphragm Myopathy

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Morfologia e expressão dos MRF’s no músculo esquelético de ratos submetidos a estímulo atrófico e recuperação por treinamento físico

Recent research advances in understanding the cellular and molecular mechanisms that underlie the processes of hypertrophy and atrophy. This may contribute to development of effective therapeutic strategies to attenuate or block the loss of muscle tissue associated with aging and pathological conditions. In this context, myogenic factors that control the activity of satellite cells have been studied to better understand the events involved in the recovery of muscle mass. Among them, we highlight the Myogenic Regulatory Factors (MRFs), which have been described as potential mediators of muscle growth. The objectives of this study evaluated the morphofunctional adaptations and gene expression of MRFs (MyoD and myogenin) in skeletal muscle (soleus) subjected to an atrophic stimulus followed by physical training. It was used 64 male Wistar rats (80 days, 250 to 300 g), divided into 8 groups (n = 8): C: control animals a week, I: Animals immobilized a week, C3: control animals 3 days; R3: Animals immobilized and recovered for 3 days, T3: Animals immobilized and submitted to exercise for 3 days; C7: Animals controls 7 days; R7: Animals immobilized and subsequently recovered by 7 days, T7: Animals immobilized and subsequently subjected to exercise for 7 days. Initially, the animals in groups I, R3, R7, T3 and T7, were submitted to 7 days of immobilization of the hind limb. Muscle atrophy was confirmed after a direct statistical comparison of the values of cross-sectional area (CSA) of muscle fibers studied in animals in groups I and C, sacrificed immediately after the immobilization period. Then, the groups T3 and T7 were submitted a rehabilitation program with muscle aerobic exercise (swimming) for 3 and 7 days respectively. The groups C, C3 and C7 were kept without stimulus atrophic and were not subjected to exercise. At the end of the experiment, the animals were sacrified and the soleus muscle removed. The quantitative analysis of gene expression ...

Efeitos do hormônio do crescimento sobre vias de sinalização intracelular na miopatia associada à insuficiência cardíaca de ratos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Blepharocheilodontic (BCD) syndrome: Expanding the phenotype?

We describe affected individuals in three generations of a family and another sporadic case, all Brazilian patients, with a combination of signs that diagnose the BCD syndrome. In addition to the cardinal signs, the sporadic case has hypothyroidism and imperforate anus, which was observed previously in one patient. The broadened phenotype and the possibility of involvement of p63 and IRF6 genes in this condition are discussed. © 2003 Wiley-Liss, Inc.

Occurrence of Cu and Cr in the sedimentary humic substances and pore water from a typical sugar cane cultivation area in São Paulo, Brazil

Purpose: The purpose of this paper is to study the interactions of sedimentary humic substances (SHS) from a sugarcane cultivation area with Cu(II) and Cr(III) and to evaluate the occurrence of these metals in the pore water and SHS. Materials and methods: For this study, the northwestern region of the State of São Paulo, Brazil, which is considered the region with the highest production of sugar cane in the state, was selected. Samples of sediment were collected from four sampling sites in the Preto, Turvo, and Grande rivers. The SHS and pore water were extracted from the sediment using the method suggested by the International Humic Substances Society and centrifugation, respectively. The complexing capacity (CC) of the SHS for Cu(II) and Cr(III) was determined by individually titrating these metals with an ultrafiltration system using tangential flow. The total concentrations of Cr and Cu were determined for the pore water, sediments, and humic substances with graphite furnace atomic absorption spectrometry and Zeeman background correction after an acid digestion, according to the methods described in US EPA Method 3050B. Results and discussion: The SHS from a site in the Turvo River, which is typically cultivated with sugarcane, possessed the highest concentration of Cu bound to SHS (25.0%), the largest CC (0.63 mmol Cu g-1 HS) and the highest concentration of this metal in the pore water (1.38 mg Cu Kg-1 sed.). For Cr, the SHS collected from a location on the Preto River dam had the largest CC (0.90 mmol Cr g-1 HS) and the lowest Cr content in the pore water (0.29 mg Cr Kg-1 sed.), indicating that there was an inverse relationship between the CC and the concentration of metal available in the pore water. Conclusions: Sedimentary humic substances might be one of the regulatory factors controlling the availability of Cu and Cr in the sediments found in a typical region that has been planted with sugarcane. Distinct behaviors were observed between the two elements investigated; higher CC and a larger fraction of Cu(II) were found in the pore water of samples originating from sugarcane crops. The opposite behavior was observed for the Cr(III) species. © 2013 Springer-Verlag Berlin Heidelberg.

Adaptações morfofuncionais e respostas moleculares do músculo esquelético de ratos submetidos ao treinamento aeróbio

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Vicilinas de leguminosas e seus efeitos no metabolismo lipídico de ratos dislipidêmicos

Pós-graduação em Alimentos e Nutrição - FCFAR

Biology and oncogenicity of the Kaposi sarcoma herpesvirus K1 protein

The Kaposi sarcoma-associated herpesvirus (KSHV), or human herpesvirus 8, is a gammaherpesvirus etiologically linked to the development of Kaposi sarcoma, primary effusion lymphomas, and multicentric Castleman disease in humans. KSHV is unique among other human herpesviruses because of the elevated number of viral products that mimic human cellular proteins, such as a viral cyclin, a viral G protein-coupled receptor, anti-apoptotic proteins (e.g. v-bcl2 and v-FLIP), viral interferon regulatory factors, and CC chemokine viral homologues. Several KSHV products have oncogenic properties, including the transmembrane K1 glycoprotein. KSHV K1 is encoded in the viral ORFK1, which is the most variable portion of the viral genome, commonly used to discriminate among viral genotypes. The extracellular region of K1 has homology with the light chain of lambda immunoglobulin, and its cytoplasmic region contains an immunoreceptor tyrosine-based activation motif (ITAM). KSHV K1 ITAM activates several intracellular signaling pathways, notably PI3K/AKT. Consequently, K1 expression inhibits proapoptotic proteins and increases the life-span of KSHV-infected cells. Another remarkable effect of K1 activity is the production of inflammatory cytokines and proangiogenic factors, such as vascular endothelial growth factor. KSHV K1 immortalizes primary human endothelial cells and transforms rodent fibroblasts in vitro; moreover, K1 induces tumors in vivo in transgenic mice expressing this viral protein. This review aims to consolidate and discuss the current knowledge on this intriguing KSHV protein, focusing on activities of K1 that can contribute to the pathogenesis of KSHV-associated human cancers. Copyright © 2015 John Wiley & Sons, Ltd.