In vitro antimicrobial activity of different gutta-percha points and calcium hydroxide pastes

The aim of this study was to evaluate the antimicrobial activity of different trademarks and compositions of gutta-percha points and calcium hydroxide pastes used in endodontic therapy. The evaluated material consisted of gutta-percha points containing calcium hydroxide (Roeko™), gutta-percha points containing chlorhexidine (Roeko™), two convencional gutta-percha points (Endo Points™ and Roeko™) and two calcium hydroxide pastes (Calen™ and Calen/PMCC™). Antimicrobial tests included five species of microorganisms: Escherichia coli (ATCC10538), Staphylococcus epidermidis (ATCC12228), Staphylococcus aureus (ATCC6538), Pseudomonas aeruginosa (ATCC27853), and Micrococcus luteus (ATCC9341). The Agar difusion method was employed. The plates were kept at room temperature for 2 h for prediffusion and then incubated at 37°C for 24 h. The triphenyltetrazolium chloride gel was added for optimization and the zones of inhibition were measured. Statistical evaluation was carried out using analysis of variance and Tukey Test. The obtained results showed that all microbial species used in the study were inhibited by the gutta-percha points containing chlorhexidine and by the calcium hydroxide pastes (Calen™ and Calen/ PMCC™), with similar results (p > 0.05). No antimicrobial activity was observed for the other groups. It was concluded that the gutta-percha points containing chlorhexidine presented antimicrobial activity, whereas the gutta-percha points containing calcium hydroxide did not.

Antimicrobial activity of Uncaria tomentosa against oral human pathogens

Uncaria tomentosa is considered a medicinal plant used over centuries by the peruvian population as an alternative treatment for several diseases. Many microorganisms usually inhabit the human oral cavity and under certain conditions can become etiologic agents of diseases. The aim of the present study was to evaluate the antimicrobial activity of different concentrations of Uncaria tomentosa on different strains of microorganisms isolated from the human oral cavity. Micropulverized Uncaria tomentosa was tested in vitro to determine the minimum inhibitory concentration (MIC) on selected microbial strains. The tested strains were oral clinical isolates of Streptococcus mutans, Staphylococcus spp., Candida albicans, Enterobacteriaceae and Pseudomonas aeruginosa. The tested concentrations of Uncaria tomentosa ranged from 0.25-5% in Müeller-Hinton agat. Three percent Uncaria tomentosa inhibited 8% of Enterobacteriaceae isolates, 52% of S. mutans and 96% of Staphylococcus spp. The tested concentrations did not present inhibitory effect on P. aeruginosa and C. albicans. It could be concluded that micropulverized Uncaria tomentosa presented antimicrobial activity on Enterobacteriaceae, S. mutans and Staphylococcus spp. isolates.

In vitro antimicrobial activity of endodontic sealers, MTA-based cements and Portland cement.

The aim of this study was to evaluate the antimicrobial activity of different root-end filling materials - Sealer 26, Sealapex with zinc oxide, zinc oxide and eugenol, white and gray Portland cement, white and gray MTA-Angelus, and gray Pro Root MTA - against six different microorganism strains. The agar diffusion method was used. A base layer was made using Müller-Hinton agar (MH) and wells were formed by removing the agar. The materials were placed in the wells immediately after manipulation. The microorganisms used were: Micrococcus luteus (ATCC9341), Staphylococcus aureus (ATCC6538), Escherichia coli (ATCC10538), Pseudomonas aeruginosa (ATCC27853), Candida albicans (ATCC 10231), and Enterococcus faecalis (ATCC 10541). The plates were kept at room temperature for 2 h for prediffusion and then incubated at 37 degrees C for 24 h. Triphenyltetrazolium chloride 0.05% gel was added for optimization, and the zones of inhibition were measured. Data were subjected to the Kruskal-Wallis and Dunn tests at a 5% significance level. The results showed that all materials had antimicrobial activity against all the tested strains. Analysis of the efficacy of the materials against the microbial strains showed that Sealapex with zinc oxide, zinc oxide and eugenol and Sealer 26 created larger inhibition halos than the MTA-based and Portland cements (P < 0.05). On the basis of the methodology used, it may be concluded that all endodontic sealers, MTA-based and Portland cements evaluated in this study possess antimicrobial activity, particularly the endodontic sealers.

Antimicrobial activity of coffee-based solutions and their effects on Streptococcus mutans adherence

The aim of this study was to evaluate the antimicrobial activity of different coffee solutions and their effects on the adherence of Streptococcus mutans to glass surface. Coffee solutions were prepared with three commercial products (Pilao, Mellita and Café do Ponto) by two different methods (simple and boiled) (n=15). A control group was also included in the study. For antimicrobial activity testing, tubes containing coffee solution and culture medium were inoculated with a suspension of S. mutans ATCC 35688 and incubated for 1 min 1h, 2h and 4h. Serial dilutions and plating on BHI agar were performed. S, mutans adherence to glass in presence of different coffee solutions was also tested. The number of adhered bacteria (CFU/mL) was determined by plating method. The results were statistically analyzed by ANOVA and Turkey's test. The tested coffee solutions did not reduce the number of colony forming units of S. mutans in relation to the control at all evaluation periods. All the solutions reduced significantly the adherence of S. mutans to the glass surface in relation to control. The tested coffee solutions did not present any antimicrobial effect on Streptococcus mutans, however, all the coffee solutions reduced significantly the adherence of S mutans to the glass surface.

Z gamma production and limits on anomalous ZZ gamma and Z gamma gamma couplings in pp collisions at root s=1.96 TeV

We present a study of eeγ and μμγ events using 1109 (1009) pb-1 of data in the electron (muon) channel, respectively. These data were collected with the DØ detector at the Fermilab Tevatron p over(p, ̄) collider at sqrt(s) = 1.96   TeV. Having observed 453 (515) candidates in the eeγ (μμγ) final state, we measure the Zγ production cross section for a photon with transverse energy ET > 7   GeV, separation between the photon and leptons Δ Rℓ γ > 0.7, and invariant mass of the di-lepton pair Mℓ ℓ > 30   GeV / c2, to be 4.96 ± 0.30 (stat . + syst .) ± 0.30 (lumi .)   pb, in agreement with the Standard Model prediction of 4.74 ± 0.22   pb. This is the most precise Zγ cross section measurement at a hadron collider. We set limits on anomalous trilinear Zγγ and ZZγ gauge boson couplings of - 0.085 < h30 γ < 0.084, - 0.0053 < h40 γ < 0.0054 and - 0.083 < h30 Z < 0.082, - 0.0053 < h40 Z < 0.0054 at the 95% C.L. for the form-factor scale Λ = 1.2   TeV.

Effects of Coffea arabica on Streptococcus mutans adherence to dental enamel and dentine

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Residual antibacterial activity of chlorhexidine digluconate and camphorated p-monochlorophenol in calcium hydroxide-based root canal dressings

The purpose of this study was to evaluate the residual antibacterial activity of several calcium hydroxide [Ca(OH) 2]-based pastes, placed in root canals of dogs' teeth with induced chronic periapical lesions. Root canals were instrumented with the ProFile rotary system and filled with 4 pastes: G1 (n=16): Ca(OH) 2 paste + anesthetic solution; G2 (n=20): Calen® paste + camphorated pmonochlorophenol (CMCP); G3 (n=18): Calen®; and G4 (n=18): Ca(OH) 2 paste + 2% chlorhexidine digluconate. After 21 days, the pastes were removed with size 60 K-files and placed on Petri plates with agar inoculated with Micrococcus luteus ATCC 9341. Pastes that were not placed into root canals served as control. After pre-diffusion, incubation and optimization, the inhibition zones of bacterial growth were measured and analyzed by Mann-Whitney U test at 5% significance level. All pastes showed residual antibacterial activity. The control samples had larger halos (p<0.05). The mean residual antibacterial activity halos in G1, G2, G3 and G4 were 7.6; 10.4; 17.7 and 21.4 mm, respectively. The zones of bacterial growth of G4 were significantly larger than those of G1 and G2 (p<0.05). In conclusion, regardless of the vehicle and antiseptic, all Ca(OH) 2-based pastes showed different degrees of measurable residual antibacterial activity. Furthermore, unlike CMCP, chlorhexidine increased significantly the antibacterial activity of Ca(OH) 2.

Anticariogenic effect of fluoride-releasing elastomers in orthodontic patients

This in vivo experimental study evaluated the efficacy of fluoride-releasing elastomers in the control of Streptococcus mutans levels in the oral cavity. Forty orthodontic patients were recruited and divided into two groups of 20. Fluoride-releasing elastomeric ligature ties (Fluor-I-Ties, Ortho Arch Co. Inc., USA) were used in the experimental group, and conventional elastomeric ligature ties (D. Morelli, Brazil), in the control group. Two initial samples of saliva were collected at a 14-day interval to determine the number of colony forming units (CFU) of Streptococcus mutans. Immediately after collecting the second sample, fluoride-releasing elastomeric ligature ties were placed in the patients of the experimental group, and conventional ligature ties, in the patients of the control group. Seven, 14 and 28 days after placement of the elastomeric ligature ties, saliva and plaque surrounding the orthodontic appliance were collected for microbiologic analysis. There were no significant differences in the number of Streptococcus mutans CFUs in saliva or plaque in the area surrounding the fluoride-releasing or conventional elastomeric ligature ties. Thus, fluoride-releasing elastomeric ligature ties should not be indicated to reduce the incidence of enamel decalcification in orthodontic patients. Since there was no significant reduction in S. mutans in saliva or plaque, other means of prevention against enamel decalcification should be indicated for these patients.

High temperature tolerance of determinate tomatoes in Brazil

The experiment was conducted in the experimental area belonging to the Section of Crop Production and Aromatic Medicinal Plants of the FCAV-UNESP, Jaboticabal Campus - Sao Paulo, Brazil. Tolerance to high temperature was studied in six determinate genotypes of tomato (Lycopersicon esculentum L.): Agrocica 8, Apex 1000, Botu-13, Calmech VFAS, Nemadoro and Jab-2, which were cultivated in a greenhouse at temperatures above 33oC for at least 2 h/day during blooming. The objectives of the study were to identify the genetic diversity of the genotypes studied and to determine their performance associated with tolerance to high temperature. Dissimilarity was determined by the generalized Mahalanobis distance. Delineation groups were optimized with the Tocher technique. A random block design was utilized with six treatments and with three replications. Two similarity groups were identified: 1 - Apex 1000, Botu-13, Calmech VFAS, Jab-2, Nemadoro and 2 - Agrocica 8. Crossing of genotypes within one group has no advantage because little genetic divergence and no heterotic response would be expected. However, the crossing of genotypes between groups is suggested. Knowledge of these groups will be important for efficiency future breeding efforts.

High temperature tolerance of indeterminate tomatoes in Brazil

The study was conducted at the Section of Crop Production and Aromatic Medicinal Plants of FCAV-UNESP, Jaboticabal Campus - Sao Paulo, Brazil. Nine indeterminate tomato (Lycopersicon esculentum Mill.) genotypes, Jumbo, Santa Clara, Cláudia VF, Concorde, Débora Plus, FM-9, Carmen, Príncipe Gigante, and CL 5915, were evaluated for high temperature tolerance. Three determinate tomato genotypes, FM-9, Suncoast and TSW-10, were cultivated in a greenhouse at more than 33°C air temperature for at least 2 h/day during bloom. The objective was to identify variable genotypes to determine their tolerance of high temperature. Four replications of fifteen treatments were planted in a randomized block design. Dissimilarity was determined by the generalized Mahalanobis distance. Delineation groups were optimized with the Tocher technique. The tomatoes were classified into six groups of similar temperature responses. CL 5915 was the most tolerant of high temperatures. Crossing of genotypes within one group has no advantage because little genetic divergence and no heterotic response would be expected. However, the crossing of genotypes between groups is suggested. Knowledge of these groups will be important for efficient future breeding efforts.

Determination of crude protein, crude fiber and crude fat in twenty-five genotypes of kale (B. oleracea var. acephala)

The aim of the study was to determine the percentage of crude protein, crude fiber and crude fat (ether extract) of 25 genotypes of kale from the Germplasm Bank of Instituto Agronômico de Campinas and of one genotype grown in the region of Jaboticabal-SP. The plants were cultivated in the field, and the leaves after collection were pre-dried in a convection oven at 65°C for 72 h. Afterward, the leaves were analyzed for crude protein, crude fiber and crude fat (ether-soluble materials). Significant differences were detected among the different genotypes for all the characteristics examined. Of the genotypes studied, six showed more than 30% crude protein: HS-20 (32.56%), Comum (31.70%), Couve de Arthur Nogueira 2 (31.16%), Pires 2 de Campinas (30.63%), Manteiga I-916 (30.36%), and Manteiga de Ribeirao Pires I-2446 (30.03%). In relation to crude fiber, the highest percentage was seen in the genotype Manteiga de Mococa (10.92%), differing significantly from the other genotypes studied. With regard to crude fat, the highest percentage was found in the genotype HS-20 (3.72%), and Pires 1 de Campinas (3.34%). Of the genotypes tested, HS-20 stood out among the others, showing both the highest percentage of protein and fat.

Search for Randall-Sundrum gravitons with 1 fb(-1) of data from p(p)over-bar collisions at root s=1.96 TeV

We search for decays of Kaluza-Klein excitations of the graviton in the Randall-Sundrum model of extra dimensions to e+e- and in 1 fb-1 of pp collisions at s=1.96 TeV collected by the D0 detector at the Fermilab Tevatron. We set 95% confidence level upper limits on the production cross section times branching fraction, which translate into lower limits on the mass of the lightest excitation between 300 and 900 GeV for values of the coupling k/M Pl between 0.01 and 0.1. © 2008 The American Physical Society.

Study of direct CP violation in B±→J/ ψK±(π±) decays

We present a search for direct CP violation in B±→J/ ψK±(π±) decays. The event sample is selected from 2.8fb-1 of pp̄ collisions recorded by D0 experiment in run II of the Fermilab Tevatron Collider. The charge asymmetry ACP(B+→J/ψK+)=+0.0075±0. 0061(stat)±0.0030(syst) is obtained using a sample of approximately 40000 B±→J/ψK± decays. The achieved precision is of the same level as the expected deviation predicted by some extensions of the standard model. We also measured the charge asymmetry ACP(B+→J/ψπ+)=-0. 09±0.08(stat)±0.03(syst). © 2008 The American Physical Society.

Search for scalar neutrino superpartners in e+mu final states in p(p)over-bar collisions at root s=1.96 TeV

We report a search for R-parity-violating production and decay of sneutrino particles in the eμ final state with 1.04±0.06fb-1 of data collected with the D0 detector at the Fermilab Tevatron Collider in 2002-2006. Good agreement between the data and the standard model prediction is observed. With no evidence for new physics, we set limits on the R-parity-violating couplings λ311′ and λ312 as a function of the sneutrino mass. © 2008 The American Physical Society.

First study of the radiation-amplitude zero in W gamma production and limits on anomalous WW gamma couplings at root s=1.96 TeV

We present results from a study of pp̄→Wγ+X events utilizing data corresponding to 0.7fb-1 of integrated luminosity at s=1.96TeV collected by the D0 detector at the Fermilab Tevatron Collider. We set limits on anomalous WWγ couplings at the 95% C.L. The one-dimensional 95% C.L. limits are 0.49<κγ<1.51 and -0.12<λγ<0.13. We make the first study of the charge-signed rapidity difference between the lepton and the photon and find it to be indicative of the standard model radiation-amplitude zero in the Wγ system. © 2008 The American Physical Society.