In vitro evaluation of root canal preparation using oscillatory and rotary systems in flattened root canals

Objective: the aim of this study was to evaluate the biomechanical preparation of flattened root canals using the following systems: Endo-Eze AET stainless steel oscillatory instruments (Ultradent) and RaCe rotary NiTi instruments (FKG Dentaire). Materials and Methods: Twenty extracted human mandibular incisors were randomly assigned to two groups: Group I Instrumentation with oscillatory Endo-Eze AET files (oscillatory technique); Group 2 - Instrumentation with rotary NiTi RaCe files (rotary technique). The teeth were decoronated, had their apices and coronal openings sealed with sticky wax and were embedded in crystal-clear orthophtalic polyester resin. The roots were sectioned transversally with diamond discs at 10 mm (middle third) and 5 mm (apical third) from the apex and the segments were reassembled for instrumentation. The sections were photographed before and after root canal instrumentation and evaluated with respect to whether the original root canal shape was modified by instrumentation. To evaluate the differences in the root canal shape before and after biomechanical preparation, scores were given regarding the instruments touch on the intracanal walls. Results: In middle third of the root canals instrumented with the rotary system, there was a change in the original canal anatomy (p < 0.05), with formation of a protuberance in the mesiodistal direction. This protuberance did not occur when the oscillatory instrumentation was used. The oscillatory system had better results in the middle and apical thirds as evaluated by Dunn's multiple-comparison test (p > 0.05). Conclusion: Under the tested conditions, Endo-Eze oscillatory system yielded the instrumentation of all flattened oot canal walls, maintaining the canal original shape throughout the biomechanical preparation, and was more effective than RaCe rotary system.

Comparative wood anatomy of root and stem in Styrax camporum (Styracaceae)

Styrax camporum Pohl is a shrub common in the cerrado vegetation of south-eastern Brazil. Root and stem wood in Styrax camporum differ quantitatively and qualitatively. Quantitative differences follow normal expectations: roots have wider and longer vessel elements, a lower vessel frequency, a lower ray frequency, and wider rays. Qualitative features of the roots are: simple perforation plates, vestured pits, and septate libriform fibres; qualitative features of the stems are: multiple perforation plates, non-vestured pits, and non-septate fibre-tracheids. Based on generally accepted evolutionary trends, root wood of Styrax camporum has more specialized features than stem wood. Additional comparative studies of stem and root anatomy are needed to determine if such differences between root and stem anatomy are widespread, and consistent with the lines of specialization observed in monocotyledons.

Topographical, diametral, and quantitative analysis of dentin tubules in the root canals of human and bovine teeth

The aim of this study was to evaluate the number and the diameter of dentin tubules in root canals, in the cervical, middle, and apical thirds, of human and bovine teeth. Twenty-four single-rooted, human premolars were divided into four groups (n = 6): GH1, 10 to 15 years; GH2, 16 to 30 years; GH3, 31 to 45 years; and GH4, 46 to 80 years; and 24 bovine incisors were divided into four groups (n = 6): GB1, central; GB2, lateral first; GB3, lateral second; and GB4, lateral third. The crowns were removed from the specimens, which were then debrided, sectioned longitudinally in the vestibular-lingual direction, and submitted to ultrasonic cleaning. Scanning electron microscopic evaluations were made with 1,000x and 5,000x magnification. According to the root thirds, statistically significant differences were found both for the number and the diameter of dentin tubules, with the cervical third presenting the highest mean values for both specimen types. As regards the number of dentin tubules, it was observed that the bovine specimens presented a significantly higher mean value than the human specimens; this difference was not observed when the diameters of the two types were compared.

Effect of a calcium hydroxide-based root canal dressing on periapical repair in dogs: a histological study

Objective. To compare the periapical repair of teeth with periapical lesion following root canal treatment by using a calcium hydroxide-based intracanal dressing for several time periods or filling in a single visit.Study design. After induction of periapical lesions in 4 dogs, the root canals were prepared using 5.25% sodium hypochlorite for irrigation, and animals were separated into 4 experimental groups; in group I, root canals were filled in a single session; in groups II, III, and IV, a calcium hydroxide-based dressing was kept in place for 15, 30, or 180 days, respectively. Root canals from groups I, II, and III were filled with gutta-percha cones and AH Plus sealer. After 180 days, animals were killed and histological sections were stained with hematoxylin-eosin to evaluate periapical repair.Results. Periapical repair was better in groups II, III, and IV (intracanal dressing) compared with group I (single session; P <.05).Conclusion. The use of a calcium hydroxide-based intracanal dressing was important for periapical repair in teeth with periapical lesion. Dressing with calcium hydroxide paste results in better periapical repair than when the root canal is filled in a single-session treatment.

rDNA-based characterization of a new binucleate Rhizoctonia spp. causing root rot on kale in Brazil

In this paper we present the first report of the occurrence of a binucleate Rhizoctonia spp. causing hypocotyl and root rot in kale in Brazil. Rhizoctonia spp. were isolated from kale (Brassica oleracea var. acephala) with symptoms of hypocotyl and root rot. The isolates, characterized as binucleate Rhizoctonia spp., did not show an anastomosis reaction with any of the binucleate Rhizoctonia spp. testers used. The pathogenicity of the isolates was tested under greenhouse conditions; all isolates were pathogenic and showed different symptom severities on kale. The ITS-5.8S rDNA sequences of kale isolates and 50 testers (25 binucleate Rhizoctonia spp. and 25 Rhizoctonia solani) were compared in order to characterize the genetic identity of Rhizoctonia spp. infecting kale. The kale isolates showed genetic identities ranging from 99.3 to 99.8% and were phylogenetically closely related to CAG 7 (AF354084), with identities of 98.5 and 98.7%. It is suggested that the binucleate Rhizoctonia spp. causing hypocotyl and root rot on kale Brazil comprises a new AG not yet described.

Root growth and cotton nutrition as affected by liming and soil compaction

Soil columns were produced by filling PVC tubes with a Dark Red Latosol (Acrortox, 22% of clay). A compacted layer was established at the depth of 15 cm in the columns. In the compacted layer, soil was packed to 1.13, 1.32, 1.48, and 1.82 Mg kg(-1), resulting in cone resistances of 0.18, 0.43, 1.20, and 2.50 MPa. Cotton was cropped for 30 days. Lime was applied to raise base saturation to 40, 52, and 67%. The highest base saturation caused a decrease in phosphorus (P) and zinc (Zn) concentrations in the plants. A decrease in root dry matter, length and surface area was also observed. This could be a consequence of lime induced Zn deficiency. Root growth was decreased in the compacted layer, and complete inhibition was noticed at 2.50 MPa. Once the roots got through the compacted layer, there was a growth recovery in the bottom layer of the pots. The increase in base saturation up 52% was effective in preventing a decrease in cotton root length at soil resistances to 1.20 MPa. Where the roots were shorter, there was an increase in nutrient uptake per unit of root surface area, which kept the plants well nourished, except for P.

POTASSIUM FERTILIZATION, ROOT MORPHOLOGY AND POTASSIUM ABSORPTION BY SOYBEAN

A greenhouse experiment studied the effect of potassium fertilization on soybean (Glycine max L. Merrill) root morphology and on K absorption by six soybean cultivars of different maturation groups and growth habits. The Plants were grown up to 70 days after plant emergence, in pots containing 6.0 kg of soil. In the absence of K, no significant difference in K absorption was observed among the cultivars or in root length and surface, but root mean radius was correlated to K absorption. Differences in K absorption were not associated with root characteristics in the presence of K fertilization. Physiological adjustments in K uptake, as well as K availability in the soil, were more important in soybean nutrition than were morphological adjustments in the root system. The results were not associated with plant growth habit or with maturation group.