Expressão da proteína nuclear de proliferação celular (PCNA), da B-Catenina e do Agnor, na etapa inicial do processo de carcinogênese quimicamente induzido pelo DMBA, em borda lateral de língua de hamsters sírio dourado(Mesocricetus auratus)

The oral cancer model in hamsters shows many simílarities with developmental oral cancer in humans. The proliferating capacity is one the most characteristics of neoplásica ce/Is and detection of these ce/Is allow us, throughout of its counting, to achieve an estimated tumour growing index, with a consequent repercussion about prognostic and in the treatment of those lesions. 40 golden Syrian hamsters, both genders, aged between two to six mouth and weight 150g in average were used. The left síde of tangue of each animal was painted for eight consecutive weeks, with a solution of DMBA. Tongues were removed, fixed in 10% buffered fonnal solution. The histological slides were stained by AgNOR technique and by PCNA and β-catenin immunohistochemical antibodies. Statistical analyzes were performed by ANOVA one-way test and Tukey test. We may conclude that an association between AgNOR and PCNA might indicate the higher proliferating activity of the analyzed celIs. The experimental carcinogenesis model in hamster tongue is an available methodology for immunohistochemistry study. And finally, PCNA and β-catenin immunohistochemical antibodies may be used to analyze possible premalignant areas in oral leukoplakia

Imunidade celular em caninos neonatos - do nascimento ao 45° dia de idade

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A interação escolar de uma criança com Sindrome de Tourette, segundo as percepções de pais e educadores um estudo de caso exploratório

The syndrome of Gilles de la Tourette (ST) can cause marked distress in several areas of functioning. The literature indicates that children with TS may present diffi culties in relationships with teachers and peers. This case study exploration aimed to discover and describe the perceptions of parents and teachers of child with TS enrolled in regular school, regarding the child-school relationships. Interviews were conducted with parents of a 8 years-old child, diagnosed with ST and three educators. Participant observation of everyday children’s school was also performed. The set of reports reveals doubts on the part of respondents to identify involuntary behaviors of the child or to identify intentional actions. It was observed that there is still no connection between parents and educators in meeting the needs of the child. It is assumed that intervention strategies in a multidisciplinary perspective could help parents, educators and health care professionals act in a coordinated way in order to improve the school and social interaction of the child.

Sistema de Comunicação Alternativa na Interação de um aluno com autismo e seu professor

Individuals with autism are often unable to communicate through speech, and the ones that are able to speak sometimes cannot begin or to maintain a conversation. This article presents a case study that observed the communicative profile of a young student with autism interacting with his teacher during classroom activities using adapted PECS. The results demonstrated that the autistic student enhanced communicative initiatives in the interaction with his teacher, and the augmentative and alternative communication system was a facilitating tool used in the communicative learning process.

Efeitos da interação e soluções utilizadas na prevenção da formação do precipitado originado pela interação do hipoclorito de sódio e do diglunato de clorexidina e a repercursão sobre a resistência de união de cimento obturador na dentina do canal radicular

Pós-graduação em Odontologia - FOAR

O leitor na reportagem de revista: um estudo das marcas da interação no gênero

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Interação genótipo vs formas de inoculação com Azospirillum brasilense em milho

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Estudo das células primárias dentais, viabilidade celular e bioatividade de materiais á base de silicato de cálcio

Pós-graduação em Odontologia - FOAR

Características da base de conhecimentos de professores no ensino de biologia celular a partir de um curso de formação continuada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Interação fungo micorrízico arbuscular e adubação fosfática no crescimento e absorção de nutrientes pelo porta-enxerto limoeiro Cravo

Estudou-se o efeito da inoculação com fungo micorrízico arbuscular (FMA), Glomus etunicatum, Glomus clarum, ou Scutelospora heterogama, e da adição de fósforo solúvel (15, 30, 45, 60, 120 e 240 mg de P kg-1 de terra) sobre as variáveis altura, número de folhas, diâmetro do caule, massas vegetais aérea e radicular, teores de nutrientes nas folhas e colonização micorrízica no porta-enxerto limoeiro Cravo (Citrus limonia (L.) Osbeck). Os FMAs, Glomus etunicatum e Glomus clarum, e doses crescentes de fósforo exerceram efeitos significativos sobre essas variáveis. O efeito da inoculação sobre as variáveis de crescimento vegetal foi mais acentuado nos tratamentos com adição de 30 a 60 mg de P kg-1 de terra. Para o teor de P foliar, o efeito da inoculação foi mais acentuado nos tratamentos adicionados de 120 a 240 mg de P kg-1 de terra. A inoculação com estes FMAs aumentou a eficiência do limoeiro Cravo em absorver nutrientes, tanto que as variáveis estudadas em plantas inoculadas na ausência de adubação fosfática superaram às de plantas não inoculadas em substrato adicionado de 240 mg de P kg-1 de terra.

Efeitos da comunicação alternativa na interação professor-aluno com paralisia cerebral não-falante

Speech has been regarded as a social communicative modality required for building relationships in different contexts and cultures. Nevertheless, this is not always a readily available resource, especially for those who have developmental disorders. In this sense, Augmentative and Alternative Communication resources and strategies have been used by nonspeaking students with disabilities as useful tools to achieve greater social participation in different environments. Therefore, the present study examined the interaction between teachers and a student with cerebral palsy before and after the use of Alternative Communication strategies in the classroom. The study had a quantitative and qualitative nature and was designed as an intervention study. There were four main stages: 1) filming before the introduction of the resources in the classroom, 2) training the school teachers 3) providing technical assistance regarding the use of alternative communication to the child's teacher 4) filming teacher-student interactions using these technological resources. The interactions were transcribed and grouped into links. In this research, a link means the initiator's behavior directed to the exchange partner and the response in sequence from the interlocutor back to the initiator. It was found that the established interactions presented a maximum of four links, with greater frequency for episodes of 1st and 2nd links. After the intervention, there were different contexts with the presence of interactions up to the 6th link, often greater frequency for episodes of 1st, 2nd and 3rd links. In addition, the teacher showed a tendency to use symbols only as a tool for evaluating and teaching concepts. This perception changed as the teacher became familiarized with the Augmentative and Alternative Communication technology resources.

Estudo etnobiológico da interação dos pescadores de cerco-fixo com as tartarugas marinhas da região de Cananéia, litoral sul de São Paulo

Marine turtles are reptiles that occur on the Brazilian coast and can travel very large distances between feeding and nesting grounds. Five species found in Brazil are threatened. The consumption of turtle meat and eggs is an ancient habit in many coastal communities around the world. The main causes that threaten these species are the increase in fishing and the drastic changes in the environment. The presence of turtles near the beaches and their accidental capture were reported by fishermen in the region of Cananéia, where this work was developed. This study was also made based in interviews of fishermen that living in the area and the follow up of their fishing activities. This methodology made possible to identify the turtles in the area, the behavior of the fishermen with these animals and the relation between non-commercial fishing and turtles. The fishing traps area has a direct relation with the capture of turtles. The higher incidence of turtle capture is usually next to islands, rocky coasts, low lands and bay entrances, which are areas where the most common species of the region (Chelonia mydas) normally look for food. The fishermen opinions about the critical situation of these species and their conservation has also been studied. Several programs in Environmental Education for the communities of the region have been recommended

Análise do envolvimento do fator de início de tradução de eucariotos 5A (eIF5A) na translocação de proteínas para o retículo endoplasmático em Saccharomyces cerevisiae

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Interação entre universidade e escola de ensino básico: um panorama dos projetos de extensão do Instituto de Biociências - Botucatu

A extensão é compreendida como uma interação entre a Universidade e a comunidade na qual está inserida e como uma via de mão dupla onde a Universidade leva conhecimentos e/ou assistência à comunidade e recebe dela influxos positivos e reais necessidades, ou seja, constitui-se uma relação de troca de conhecimentos. As atividades de extensão desenvolvidas no Instituto de Biociências (IB) da UNESP - Campus Botucatu, por meio de projetos, são diversificadas quanto ao público alvo, quanto ao local de realização e também quanto aos objetivos. Ao longo dos anos, foram realizadas inúmeras atividades de extensão dirigidas aos professores e alunos das escolas de ensino básico, das quais destacamos os projetos de extensão relacionados ao ensino de Ciências e Biologia. Este estudo teve por objetivos identificar as atividades de extensão, desenvolvidas por docentes do IB, voltadas ao ensino básico, e investigar a percepção dos docentes sobre a articulação entre Universidade e escolas de ensino infantil, fundamental e médio. Os dados foram coletados por meio de análise de documentos, questionário e entrevista. Pela análise de documentos, foram identificados os projetos de extensão relacionados ao ensino de Ciências e Biologia e que possuem relação (direta ou indireta) com escolas do ensino básico desenvolvidos pelo IB nos anos 2006, 2007 e 2008, cadastrados na PROEX, vinculados ao Núcleo de Ensino, ao Programa Ciência Na UNESP e ao NADi - Museu Escola. Por meio do questionário, com questões abertas e fechadas, foram coletados dados junto a 15 professores coordenadores e pela entrevista foram coletados dados junto ao Presidente da Comissão Permanente de Extensão Universitária. Segundo os dados obtidos, foi possível perceber um aumento no número de projetos ao longo dos três anos, assim como um aumento na participação de professores e alunos do Instituto...(Resumo completo, clicar acesso eletrônico abaixo)

Aplicações de um potencial reativo no estudo da interação entre moléculas e superfícies bidimensionais

In materials science, the search for technological improvements have become one of the main subject of study of researchers. This is especially true in the case of materials with reduced sizes, in the nanometer scale. Important phenomena to be studied in these cases are the desorption and adsorption on two-dimensional materials, such as graphene. These phenomena are of great importance in the study of interactions between organic films, synthesis or catalysis of reactions on surfaces and even in the creation of nanoscale devices [1, 2, 3, 4]. Between the most important topics related to these phenomena are the storage of gases in low-dimensional systems and the study of nanostructured fuel cells or batteries. In this context we used two different parametrizations for the reactive force field ReaxFF to study the potential barriers and reaction barriers of our system. First we made a study about the Reaction Barriers and Energy Barriers for bonds between graphene and the following atoms: sulfur, fluorine, hydrogen, nitrogen and oxygen. It is important to have this information in order to make it possible to understand how these atoms react with the graphene sheet. Subsequently, we calculate reaction barriers for mixed structures where fluorine is a fixed element bonded to graphene and other element is simultaneously bonded to graphene. This other element (N, O, H or S) is varied in its possible relative positions (ortho, meta and para in relation to fluorine in either: the same side and in the opposite side of the graphene membrane)