Adhesion between fiber post and root dentin: evaluation of post surface conditioning for bond strength improvement.

The aim of this paper was to evaluate two surface conditioning methods associated with the application of adhesive on the post surface for improving the bond to resin cement. Sixty single-rooted bovine teeth were sectioned at 16 mm in length, prepared (9 mm depth), embedded in a PVC cylinder using acrylic resin, and allocated into 3 groups (N.=20) according to post surface treatment: cleaning with ethanol (control group); etching with hydrogen peroxide; etching with hydrofluoric acid. Ten posts for each group were silanized and other 10 posts were silanized and received an adhesive agent. The posts were cemented with self-adhesive resin cement (RelyX U100 resin cement). All teeth were sectioned perpendicularly to the long axis (2 mm thickness per slice), submitted to push out bond strength testing and the type of failure was recorded. The obtained data were submitted to two-way ANOVA and Turkey's test, with the level of significance set at 5%. Neither the hydrofluoric acid or hydrogen peroxide post surface treatment, nor the adhesive application, had an influence on bond strength values. The main type of failure was adhesive between cement and dentin. Etching and the application of an adhesive on the post surface did not presented a significant influence on the bond strength results for the fiber post resin cement-root dentin assembly. The cement appears to adhere very well to the fiber post surface rather than the dentin surface.

Root distribution, nutrient uptake, and yield of two upland rice cultivars under two water regimes

Upland rice (Oryza sativa L.) cultivation has been increasing in global importance due to the decreasing water availability for flood- irrigated rice. The use of sprinkler irrigation to supplement rainfall and the identification of cultivars more adapted to lower water availability could be effective alternatives for producing upland rice without yield losses while using less water. The objective of this field study was to evaluate the root distribution, plant nutrition, and grain yield of two drought tolerant upland rice cultivars under two water regimes in the Cerrado Region of Brazil during two growing seasons. The main plots were two water regimes (rainfed and sprinkler-irrigation plus rainfall). Subplots were two upland rice cultivars Carajás and IAC 201. Low water availability reduced root growth by 7% and grain yields were from 2644 to 4002 kg ha-1 on average for rainfed and sprinkler irrigation treatments, respectively. Carajás had a significantly better root distribution, nutrient uptake, and higher grain yield (3732 kg ha-1) compared with IAC 201 (2914 kg ha-1) averaged over two growing seasons and water regimes. There were no treatment interactions. Our results suggest that, even when cultivars with a higher tolerance to less water availability are used, using sprinkler irrigation to augment limited rainfall during dry periods may be a viable method to increase upland rice grain yields. © 2013 by the American Society of Agronomy.

Seedling morphology and development in the epiphytic cactus Epiphyllum phyllanthus (L.) Haw. (Cactaceae: Hylocereeae)

Seedling morphology is relevant in classification, taxonomy, and vegetation studies to understand plant life cycles, germination succession and requirements, and developmental progression. However, most morphological studies of seedlings lack analysis of organ anatomy, impeding the comprehension of series of development and establishment in a particular environment. Here, we have taken a traditional anatomical approach to examine the stages of seedling development in Epiphyllum phyllanthus, a holo-epiphytic cactus of tribe Hylocereeae. The goals were 1) to offer a comprehensive description of growth series in E. phyllanthus seedlings based on morphological and anatomical analysis and 2) to examine the initial growth phases in the life cycle of this species to identify organ development and understand their adaptive significance in relation to seedling establishment. Our results include descriptions of seed morphology, embryonic features, and seedling vascularization pattern in the root, hypocotyl, cotyledons, and epicotyl. The morphological and developmental patterns in E. phyllanthus seedlings have potential phylogenetic and ontogenetic implications in the Cactaceae. Characters such as the presence of mucilage on the seed coat, the lack of seed operculum, and large cotyledons in E. phyllanthus are comparable to basal cacti, but the root anatomy is more similar to columnar relatives. At the familial level, there is an apparent trend in decreasing number of phloem and xylem poles in the stele of primary root, correlated with degree of specialization and advanced phylogenetic position: tetrarch to septarch-octarch in basal lineages, tetrarch Cereus-type in columnar species, to the diarch vascular system in Rhipsalideae and some species with cylindric/globose stem. © Torrey Botanical Club.

Motor Recovery and Synaptic Preservation after Ventral Root Avulsion and Repair with a Fibrin Sealant Derived from Snake Venom

Background:Ventral root avulsion is an experimental model of proximal axonal injury at the central/peripheral nervous system interface that results in paralysis and poor clinical outcome after restorative surgery. Root reimplantation may decrease neuronal degeneration in such cases. We describe the use of a snake venom-derived fibrin sealant during surgical reconnection of avulsed roots at the spinal cord surface. The present work investigates the effects of this fibrin sealant on functional recovery, neuronal survival, synaptic plasticity, and glial reaction in the spinal motoneuron microenvironment after ventral root reimplantation.Methodology/Principal Findings:Female Lewis rats (7 weeks old) were subjected to VRA and root replantation. The animals were divided into two groups: 1) avulsion only and 2) replanted roots with fibrin sealant derived from snake venom. Post-surgical motor performance was evaluated using the CatWalk system twice a week for 12 weeks. The rats were sacrificed 12 weeks after surgery, and their lumbar intumescences were processed for motoneuron counting and immunohistochemistry (GFAP, Iba-1 and synaptophysin antisera). Array based qRT-PCR was used to evaluate gene regulation of several neurotrophic factors and receptors as well as inflammatory related molecules. The results indicated that the root reimplantation with fibrin sealant enhanced motor recovery, preserved the synaptic covering of the motoneurons and improved neuronal survival. The replanted group did not show significant changes in microglial response compared to VRA-only. However, the astroglial reaction was significantly reduced in this group.Conclusions/Significance:In conclusion, the present data suggest that the repair of avulsed roots with snake venom fibrin glue at the exact point of detachment results in neuroprotection and preservation of the synaptic network at the microenvironment of the lesioned motoneurons. Also such procedure reduced the astroglial reaction and increased mRNA levels to neurotrophins and anti-inflammatory cytokines that may in turn, contribute to improving recovery of motor function. © 2013 Barbizan et al.

Phytotoxicity of organic extracts of Turnera ulmifolia L. and Turnera diffusa Willd. ex Schult. in cucumber seeds

We evaluated the phytotoxic effects that the hexane, ethyl acetate and methanol extracts from leaves and branches of the species Turnera ulmifolia L. and Turnera diffusa Willd. ex Schult. (Turneraceae), at concentrations of 1.25, 2.50, 3.75 and 5.00 mg ml-1, have on seed germination and seedling development in cucumber (Cucumis sativus L.). None of the extracts tested prevented germination, although the ethyl acetate extracts of T. diffusa, at 3.75 and 5.00 mg ml-1, reduced the mean germination speed and time to germination. Hexane extracts of both species reduced the main root length, number of secondary roots and hypocotyl length. In the ethyl acetate and methanol extracts, the number of secondary roots and hypocotyl length varied by species and concentration. Ethyl acetate extracts of T. ulmifolia at ≤ 2.50 mg ml-1stimulated growth of the main root and hypocotyl, as did T. ulmifolia methanol extracts at 1.25 and 3.75 mg ml-1; all other extract/concentration combinations had an inhibitory effect on those parameters. The hexane and ethyl acetate extracts of T. diffusa inhibited the formation of secondary roots and of the main root, although significant inhibition of hypocotyl growth was observed only at ≥ 2.50 mg ml-1. Comparatively, T. diffusa extracts inhibited development to a greater degree, thus presenting greater allelopathic potential, than did T. ulmifolia extracts.

Análise de imagens no estudo da porosidade em latossolo cultivado com batata (Solanum tuberosum L.) sob dois sistemas de irrigação

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Comportamento de milho (Zea Mays L.) e propriedades físicas do solo, no sistema plantio direto, em resposta a aplicação de fertilizante organomineral

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Teste de deterioração controlada para avaliação do vigor de sementes de milho (Zea mays L.)

Pós-graduação em Agronomia (Agricultura) - FCA

Efeitos do extrato bruto e da microcistina-LR em Lactuca sativa L. (Asteraceae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produção de biomassa, rendimento e composição do óleo essencial de manjericão (Ocimum basilicum L.) em função de reguladores vegetais

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento do sistema radicular do milheto (Pennisetum americanum L.) em duas classes de solo em densidade e diferentes profundidades da camada compactada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desenvolvimento do sistema radicular de amaranto (Amaranthus cruentus, variedade BRS Alegria), de milheto (Pennisetum glaucum (L.), variedade BN-2) e de pé de galinha (Eleusine coracana L.) em duas classes de solo e quatro densidades

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desenvolvimento de larenjeira 'Pera' Citrus Sinensis (L.) Osbeck enxertada em limoeiro 'Cravo' (Citrus Limonia) e cultivada em pó de balsalto

Pós-graduação em Ciências Biológicas (Botânica) - IBB

Otimização de protocolos de germinação in vitro de pinhão-manso (Jatropha curcas L.)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização morfológica, citogenética e palinológica de genótipos de feijão-vagem (Phaseolus vulgaris L.) - Fabaceae

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)