Organização genômima e análise da expressão do gene hnRNP Q-like (Heterogeneous nuclear ribonucleoprotein A-like) retroinserido no cromossomo B de Astatotilapia latifasciata

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Pesquisa dos genes icaD e bap de adesão intercelular bacteriana e formação de biofilme por Staphylococcus aureus e Staphylococcus coagulase-negativa isolados de mastite bovina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Caracterização das espécies de leishmania em sangue periférico de cães por PCR-RFLP NA área endêmica de Bauru/SP

Pós-graduação em Ciência Animal - FMVA

Comparação entre métodos diagnósticos da tuberculose em bovinos abatidos em matadouros-frigoríficos do Estado de São Paulo

Pós-graduação em Medicina Veterinária - FCAV

Expressão imuno-histoquímica de KI-67, COX-2, MMP-9 e P53 nos tumores testiculares caninos

Pós-graduação em Ciência Animal - FMVA

Detecção do herpes vírus felino tipo 1 (HVF-1) pela técnica de PCR em tempo real e sua associação com sinais oculares em uma poipulação de gatos domésticos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Validação da intradermoreação de Montenegro para diagnóstico de leishmaniose em felinos

Pós-graduação em Ciência Animal - FMVA

Research of Klebsiella pneumoniae in dairy herds

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Serological and molecular survey of Leptospira spp. among cart horses from an endemic area of human leptospirosis in Curitiba, southern Brazil

Introduction: Cart horses are a re-emerging population employed to carry recyclable material in cities. Methods: Sixty-two horses were sampled in an endemic area of human leptospirosis. The microscopic agglutination test (MAT) and real-time polymerase chain reaction (qPCR) were performed. Results: A seropositivity of 75.8% with serovar Icterohaemorrhagiae in 80.8% of the horses was observed. Blood and urine were qPCR negative. MAT showed positive correlations with rainfall (p = 0.02) and flooding (p = 0.03). Conclusions: Although horses may be constantly exposed to Leptospira spp. in the environment mostly because of rainfall and flooding, no leptospiremia or leptospiruria were observed in this study.

MicroRNoma do carcinoma de pulmão de células não pequenas

Pós-graduação em Bases Gerais da Cirurgia - FMB

Prevalência de coinfecção e fatores associados ao Papilomavírus Humano (HPV) e à Chlamydia tracomatis em mulheres em idade reprodutiva atendidas em Program de Prevençao de Câncer do Colo Uterino na região sudeste do Brasil: sub-titulo / (se houver)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Transcrição cooperativa de genes ribossomais em Escherichia coli usando um modelo estocástico e dependente de sequência

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Detecção do Paracoccidioides spp. em amostras ambientais e diferenciação do complexo P. brasiliensis da espécie P. lutzii por Nested PCR e hibridização in situ

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da diluição do DNA extraido de material parafinado para amplificação em PCR

Introduction: Several reasons may lead to the failure of polymerase chain reaction (PCR) using DNA purified from paraffin-embedded materials: presence of inhibitors and degradation of target DNA. DNA dilution will often reduce the concentration of potential inhibitors and still contain enough DNA to allow PCR amplification. Objective: To evaluate the dilution influence of DNA purified from paraffin-embedded materials on β-globin PCR amplification. Material and Method: Paraffin-embedded blocks from 30 patients with oropharynx squamous cell carcinomas, diagnosed and treated at the Oral Oncology Center were selected. DNA extraction was performed using QIAmp minikit (Quiagen). DNA was quantified and evaluated for purity by spectrophotometer analysis. Two groups were formed with different amounts of DNA: group I had the originally extracted DNA and group II had the same DNA, however diluted with ultrapure water addition. PCR was performed in both groups using oligonucleotides for human β-globin gene. Results: For Group I, amplification of the β-globin gene sequence was successful in 33.33% of the samples and for Group II, in 23.33%. Conclusion: Dilution of the DNA extracted of paraffin-embedded materials did not modify statistically the amount of positive samples β-globin gene amplified in PCR, although the results suggest that this is a way to increase the method for efficacy amplification of PCR.

Detecção de microrganismos de infecções bucais: perspectivas e cuidados a serem seguidos

The diagnosis of head and neck infections constitutes relevant step in their treatment. However, in spite of the fact that most of diseases in head and neck region are infectious in nature, several reasons collaborated for dentists do not ask laboratory tests in order to help clinical diagnosis. By mean of this review literature, based on research articles about the newest and most reliable methods of diagnosis for clinical laboratories, the authors discuss the advantages and disadvantages of each selected method and the relevant aspects in transportation of the specimens to the laboratory. Saliva, biofilm, pus, and blood are the most frequent specimens for microbial diagnosis, being that the most used methods are culture and those based on detection of deoxyribonucleic acid by polymerase chain reaction method. Whereas, the culture depends on cellular viability, and has reduced sensitivity, as well as needs favorable conditions in the sample collection and transportation, PCR shows high sensitivity and specificity, but it does not allow the determination of antibiogram, what reduces its usefulness. In addition, few laboratories possess conditions to perform cultivation of obligate anaerobes or have experience in the molecular detection of these microorganisms.