Padronização da técnica de PCR em tempo-real na avaliação da pluripotência de células-tronco mesequimais caninas

Este relatório final tem como objetivo apresentar as atividades desenvolvidas no período de janeiro/2009 a março/2010, pela aluna Thaila Isabel Wodewotzky, relativas ao projeto de conclusão de curso intitulado “Padronização da Técnica de PCR em tempo-real na Avaliação da Pluripotência de Células-tronco Mesenquimais Caninas”, para fins de obtenção do título de Bacharel em Ciências Biológicas. O referido projeto objetiva avaliar a quantificação e relevância dos níveis de expressão gênica do fator de transcrição Oct4 em CTM´s, por meio da padronização da técnica de PCR em tempo-real. Para tanto, o RNA total das CTM´s obtidas, isoladas e cultivadas a partir da medula óssea de cães foi extraído a partir da medula óssea de cães a fim de avaliar a quantificação e relevância dos níveis de expressão gênica do Oct4 por meio da utilização da técnica de PCR em tempo-real com transcrição reversa (RT-qPCR). O RNA total foi extraído e submetido à reação de transcriptase reversa, para a obtenção do cDNA. Posteriormente esse cDNA foi utilizado na padronização da técnica de qPCR utilizando primers desenhados a partir de sequências obtidas no genebank. . Como normalizador utilizou-se o RNA codificante de GAPDH.Verificou-se desempenho satisfatório dos primers para Otc4 na avaliação de CTM´s de cães. Também o RNAm do GAPDH foi adequado como normalizador. Dessa forma, esse sistema pode ser utilizado na realização de testes quantitativos utilizando amostras de células-tronco embrionárias caninas

O mal-estar docente e os limites da experiência no tempo presente: uma leitura frankfurtiana

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Um tempo para a literatura ou a vida entre o labirinto e a bússola

Since the priors, man has been trying to understand the concept of time. From myth to quantum physics, time is something that inspires reflections on our own lives. Do we exist in time or for time? Literature, a peculiar form of knowledge, deals with the experience of time in many ways. By avoiding categorizations, literature converges time and space in a dimension in which labyrinth and compass converge: at the reading time, in the reader’s space, in the universe of the book, where Cronos, Kairos, and their heir, by excellence, the literary word, constellate. In this way, in this essay, I discuss some aspects of the relationship between literature and time, or still, what literature can teach us about the time.

Degradação da manipueira utilizando reator de lodo ativado em batelada sequencial com elevado tempo de paralisação intermitente da aeração

Conselho Nacional de Desenvolvimento em Pesquisa (CNPq)

Machado de Assis, jornalista: o homem, o texto, o tempo.

This article analyses some of Machado de Assis’ newspaper chronicles written in 1878 for O Cruzeiro. We intend to show that behind their extremely free literary composition, those texts show the author’s deepest critical point of views. Respecting the narrator fictional status, we are trying to see, as carefully as this subject demands on us, the man behind the journalist.

Estudo para correção dos sensores da rede de monitoramento do campo elétrico em função do campo elétrico de tempo bom

A monitoring network of atmospheric electric field covering the Vale do Paraiba region was implemented. The sensors were located on different sites with different altitude and geographic topology. The present work reports the study carried on those sensors in order to verify the necessity of using some correction factor to the measured local electric field intensity due to effects of local environment. The measurements were done in continuous 24 hours per day with the data recorded on registers in each device accumulating information during a period of four months. The relation between the electric field values by each sensor was compared to the reference located on Sao Jose dos Campos city using the same period. In a graphical analysis using the local field intensity and the reference, the data were fitted to a straight line obtained by minimum square method. Variation up to 95% was observed between the field values in some sensors. Another method was proposed, comparing the mean values of the electric field in a function of time. The variation in some sensors reached up to 133%. We conclude that the variations are due to local atmospheric conditions and no correction factor is required on the electric field sensors

Vida e morte em diálogo com a voz narrativa, o tempo e o espaço em Mrs. Dalloway, To The Lighthouse e Between the Acts de Virginia Woolf

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Determinação do tempo de vida de restaurações totalmente cerâmicas (y-tzp/porcelana) com incidência da carga em diferentes regiões de contato

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito da escovação utilizando dentifrícios com diferentes graus de abrasividade no desgaste do esmalte após o uso de agentes clareadores com e sem cálcio, em diferentes intervalos de tempo

Pós-graduação em Odontologia Restauradora - ICT

Validação de técnicas moleculares para o diagnóstico de bactérias em peixes, visando redução de tempo e custo

Pós-graduação em Microbiologia Agropecuária - FCAV

Tempo Ideal para a coleta do sopro no teste respiratório com isótopos estáveis (13C) para o diagnóstico do Helicobacter pylori

A study was conducted to determine the optimal time for collecting the breath examination urea breath marked with the stable isotope 13C. We selected patients before undergoing the examination of endoscopy at the Endoscopy Section of the University Hospital of Botucatu - SP. A screening was performed to determine which patients wanted and could participate. Before performing endoscopy basal sample was collected from the patient and then the labeled urea ingested. The blows were collected in double every 2.5 minutes until an interval of 30.0 minutes after were collected every 5.0 minutes until the time of 45.0 minutes . The samples were analyzed in a mass spectrometer for isotope ratio, located in the center of Stable Isotopes, Institute of Biosciences, UNESP - Botucatu campus. The data were studied and arranged in the form of graphics to better interpretation of results. Based on the obtained results it was determined that a standby time of 15.0 minutes to collect the wind is sufficient for accurate diagnosis and effective

Tempo(s) e alteridade: a escuta nas aulas de filosofia com crianças

Children perceive philosophy classes as space and time in which they can speak whatever they think and they like. ! at is a fact: philosophy classes for and with children open space to speak and to think. But there is a distance between speak and be heard out. Which kind of hearing is interesting in this type of class? Hearing assumes a real meeting between persons and this takes for granted that someone is also and essentially interested in the other’s thought . Such questions take us to have a problem with our concept of childhood. Recovering debates made by Walter Omar Kohan about childhood and temporality we try to connect the questions we indicate here and their meaning in the classes of philosophy for children keeping as our aim to the liberating possibility to children and teachers.

O tempo de O despertar

Departing from the theoretical principle that the feminine text, as a palimpsest, holds subtextual meanings which, in permanent interaction with the textual surface, disarticulate the oppositional and hierarchical backgrounds of patriarchy, this essay intends to offer a general overview on the narrative instance of time in The Awakening, the most important work by Kate Chopin – one of the highlights of Realism in the United States –, with the intention to show how the time instance brings with it and at the same time disseminates into the other narrative elements some inter-dictions to gainsay patriarchy.

Localizando o pretérito perfecto compuesto na linha do tempo: o estágio da gramaticalização do PPC nas variedades diatópicas argentinas

The aim of this study was to observe Argentine diatopic varieties and to identify in which stage of the evolution of the pretérito perfecto compuesto (PPC) the use of compound tense fits into these variations. Therefore, we assume as assumptions (I) some authors` description of the evolutionary stages of this verb tense in Spanish, (II) the description of the values assigned to the perfecto compuesto in the dialect regions of Argentina and (III) the contributions of studies on grammaticalization for the verb tenses analysis.

A ironia de Geni e o Zepelim: sujeitos, poderes e mundos no tempo da suspensão

The purpose of this article is to analyze the song "Geni e o Zepelim", by Chico Buarque, from the point of view of the social voices present and orchestrated by the narrator. The hypothesis is that the image of the song uncovers the nation´s prejudice by criticizing the hypocrisy conducted by the narrator. In order to do so the song is used as the corpus of analysis, which is taken as a discursive arena where social voices discuss and combat. Therefore, it is possible to analyze the criticism regarding prostitution prejudice and Geni a transvestite inflicted ironically by the narrator’s voice – projected by the authorship in the song. Based on the dialogism present in the subjects voices in the lyrics, considering Bakhtin´s Circle perspectives. The center of analysis is the ironic relationships of power because the subjects position symbolize distinct social power spheres, which by irony are relativized. Therefore, thinking about these relations means reflecting about the construction of a certain speech in time and space, which collaborates with the reconstruction of subjects and social realities.