Análise da expressão do gene CaPox que codifica uma peroxidase de classe III envolvida na interação do cafeeiro com nematóides

A caracterização e isolamento do gene com expressão específica em raiz de café (Coffea arabica) que codifica uma peroxidase (CaPOX) e suas respectivas regiões promotoras, permitiu realizar a caracterização da expressão desse gene em reposta a estresse biótico (infecção por nematóides) assim como a análise funcional do seu promotor. Promotores tecido-específicos responsáveis pela regulação de genes responsivos a estresses bióticos tornam-se fundamentais em programas biotecnológicos que visam o aumento da resistência e tolerância vegetal. Partindo desse princípio, realizou-se a quantificação da expressão relativa do gene CaPOX em raízes de café utilizando plantas de Coffea arabica de um cultivar susceptível (Mundo Novo) e de outro cultivar resistente (IAC 388-17-1) a nematóides, respectivamente. Em paralelo utilizou- se plantas transgênicas de tabaco (Nicotiana tabacum SR1) contendo a versão completa do promotor do gene CaPOX em fusão transcricional ao gene repórter uidA (que codifica a β-glucuronidase; GUS) . A partir disso, pode-se observar que o gene CaPOX tem sua expressão aumentada em resposta a infecção por nematóides, sendo que a indução observada ocorre nos tempos iniciais pós-inoculação. Da mesma maneira, o promotor do gene CaPOX é responsivo a infecção por nematóides, sendo ativado nos tempos iniciais pós-inoculação

Remoção de matéria orgânica e nitrogênio de efluentes provenientes de abatedouros de bovinos por meio de reator aeróbio operando em bateladas sequenciais com adição de coagulante químico

The volume of liquid effluent generated in cattle slaughterhouses is quite high and cannot be released untreated in water bodies due to its high pollution load of predominantly organic origin. To minimize the environmental impacts of its industrial wastewater and meet the local environmental legislation, abattoirs shall make the treatment of these effluents. The present work aims to develop the study of a reactor by sequential batch pilot scale, in order to optimize their performance in treating wastewater from a cattle slaughterhouse. The treatment system used was developed and installed in the Laboratory of Wastewater Treatment, in Faculty of Science and Technology UNESP, Presidente Prudente campus. The procedure used followed the operation of sequential batch reactors, in which all processes and treatment operations occurring sequentially in a single unit, by establishing specific operating cycles, which comprise the following separated phases: aerobic reaction, anoxic reaction, sedimentation and emptying. Aiming to improve the quality of treatment was planned the addition of coagulant Poly Aluminum Chloride (PAC) in the reactor, by determining their optimal dosage by Jar-test trials. Were prepared four steps with specific operating cycles: step one or acclimatization (10 hour of aeration, one hour and 30 minutes of sedimentation and 30 minutes for exchanging the effluent); step 2 (6 hours of aeration or aerobic phase, 4 hours and 45 minutes of stirring or anoxic phase and 1 hour and 15 minutes for sedimentation and exchange effluent); step 3 (2 hours and 30 minutes of aeration, 8 hours and 15 minutes of stirring and 1 hour and 15 minutes for sedimentation and exchange) and step 4 (2 hours of aeration, 8 hours and 45 minutes of stirring and 1 hour and 15 minutes for sedimentation and exchange)... (Complete abstract click electronic access below)

Estudo da fotoisomerização em filmes de polímero triacrilato dopado com azocorante disperse red 1

Triacrylate resins are widely used to fabricate 3D microstructures, photonic crystals and optical devices. These resins can be doped with photosensitive materials like Disperse Red 1 (DR1), an Azo dye that changes its molecular configuration from trans to cis (photoisomerisation) with variations in their optical absorption spectrum when irradiated with ultraviolet or visible light. The reversibility of this process is thermally activated and occurs when the molecule remains at dark. In this work was prepared a 1:1 mixture of ethoxylated-6-trimethylolpropane (SR499) and tris-2-hydroxyethyl-isocyanurate (SR368) triacrylate resins, plus photoinitiator Lucirin TPOL, forming a UV curable polymeric matrix doped with DR1. Thin films were deposited onto glass slides by spin-coating technique. The films showed photochromic effect when illuminated with a low power diode laser (450 nm and 50mW/cm2). This effect is reversible after sample is left in dark. Thermochromic effect was evaluated by optical absorption measurements in the range of 40 to 140oC, heating the samples in-situ

Remoção de N-amoniacal em baixas concentrações de oxigênio dissolvido a partir do efluente de frigorífico bovino

This work aimed to develop a biological system for removal of ammonia nitrogen operating at low concentrations of dissolved oxygen. Thus, a biological upflow vertical reactor was built, in which the affluent pass through the support media until the top. Sludge from an anaerobic stabilization pond of a slaughterhouse unit in the city of Presidente Prudente - SP was used as inoculum. Initially the system operated in batch and afterwards in a continuous flow with different HRT. For feeding the reactor, an initial phases was adopted a synthetic culture media, described by Martins (2007), in order to establish the ideal conditions for the development of Anammox bacteria and subsequently, submitted to the system a slugde effluent of slaughterhouse. The results showed significant removal efficiency of N-NH4+, especially in the phase without recirculation of culture media, with an average of 71% removal, with the proportion of removal of N-NH4 +:N-NO2- average 1: 1,69. For the period of operation with effluent from the slaughterhouse, were not obtained satisfactory results, without confirmation of the proliferation of Anammox bacteria in the system, due to the high presences of organic matter in the same confirmed by high concentrations of COD

Biosusceptometria de corrente alternada para avaliação do trânsito gastrintestinal de alimentos líquidos em ratos

The gastrointestinal tract is the main route of nutrients absorption and drugs delivery. Is important to know the parameters related to the tract, like gastric emptying and gastrointestinal transit, in order to better understand the behavior of different kind of meals or drugs passing through the GIT. Many techniques are used to study these parameters, such as manometry, scintigraphy, phenol red, activated charcoal and carbon-13 reading. However, these methods use radiation, are invasive and require animal sacrifice. As an alternative proposal, the Alternate Current Biosusceptometry (ACB), a magnetic technique, has proved to be effective for these studies with small animals, in a noninvasive way, low cost, radiation free and avoiding the animal death. Associating the ACB to magnetic micro or nanoparticles used as tracers, it is possible to observe the meal behavior inside of the GIT. Focusing meanly on liquid meals digestion, this paper had the objective to evaluate the efficiency of the ACB technique in gastric emptying and gastrointestinal transit evaluation of liquid meals in rats. To perform the experiments, magnetic nanoparticles (ferrite, MgFe2O4) were used on a 1,5 ml solution introduced by gavage on similar weight and age rats. The sensor made by 2 pairs of coils, capable of generating and detecting magnetic fields, creates a field on the interest place and when this field is in contact with the marked meal, it changes, resulting on a variation of the measured voltage. The voltage variation is analyzed and is obtained a particle concentration on the interest region. The results showed that is possible to apply the ACB technique on the GIT evaluation of liquid particles digestion, gastric emptying and meal cecum arrival time curves were obtained and from that, is possible to observe a pattern of gastrointestinal transit. Both mean process time values were acquired, proving the technique capability of ...

Efeito da ativação física e química do gel clareador na velocidade de penetração do peróxido de hidrogênio: estudo in vitro

This study evaluated the effect of physical and chemical activation on the speed of penetration of hydrogen peroxide bleaching agents present in different concentrations through the enamel and dentin. One hundred and twenty bovine incisors were used, which were obtained enamel/dentin discs of the buccal surface, with 6 mm in diameter. The samples were divided into six groups: G1 - Hydrogen Peroxide Gel 20%, G2 - Hydrogen Peroxide Gel 20% with light activation, G3 - Hydrogen Peroxide Gel 20% with Manganese Gluconate; G4 - Hydrogen Peroxide Gel 35%; G5 - Hydrogen Peroxide Gel 35% with the light activation and G6 - Hydrogen Peroxide Gel 35% with Manganese Gluconate. The specimens were placed in a transparent support on which there was a substance sensitive to hydrogen peroxide immediately below and in contact with the specimen. After the procedures for applying the gel for each group, one video camera was positioned and operated to monitor the time of penetration of peroxide in each specimen. The recording ended after changing the color of the fluid revealed in all specimens and times were noted for comparison. ANOVA analysis showed that concentration and type of activation of bleaching gel significantly influenced the diffusion time of hydrogen peroxide (P 0.05). 35% hydrogen peroxide showed the lowest diffusion times compared to the groups with 20% hydrogen peroxide gel. The light activation of hydrogen peroxide decrease significantly the diffusion time compared to chemical activation. The highest diffusion time was obtained with 20% hydrogen peroxide chemically activated. The diffusion time of hydrogen peroxide was dependent on activation and concentration of hydrogen peroxide. The higher concentration of hydrogen peroxide diffused through dental tissues more quickly

Análise do corpo gorduroso do diplópodo Rhinocricus padbergi para verificação de seu potencial bioindicador de solos impactados

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Terapia fotodinâmica - Aplicações em lesões cutâneas

Photodynamic therapy is a method of healing skin lesions , in addition to radiotherapy and brachytherapy are commonly used in the treatment of skin cancers , but it is still little known in Brazil , consisting of the application to the skin of a drug in the form of cream or lotion ( photosensitizer ) . It is absorbed by tumor cells and subsequently activated by light device , that wavelength of light in the order of 630 nanometers , promoting the selective destruction of cancer cells while preserving healthy cells around the lesion . It can be considered highly effective for treatment of skin cancer types of non-melanoma ( basal cell carcinoma , 85-100 % success in curing [ 3 ] , and Bowen's disease), actinic keratoses , which are precancerous lesions and still no studies that prove also action in the treatment of acne and aging caused by the sun . And the advantage of this treatment is that , when indicated , replace the surgical procedure , selectively treating the diseased cells . Thus, it offers better cosmetic results, reducing the risk of scarring [ 4 ]

Avaliação da toxicidade do azocorante Acid Red 114 antes e após processo de biodegradação por meio de um consórcio de microorganismos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo sobre desruptores endócrinos em sistemas aquáticos: detecção e perspectivas de tratamento das águas do Rio Aporé-MS/GO, utilizando-se adsorventes sólidos

Pós-graduação em Ciência dos Materiais - FEIS

Disponibilidade de fósforo em solo tratado com resíduos urbanos e industriais sob sistema de plantio direto cultivado com soja

Pós-graduação em Agronomia (Agricultura) - FCA

Efeito do tipo de adesivo e do seu mecanismo de ativação, assim como da viscosidade da resina composta, na resistência de união à dentina antes e após envelhecimento artificial

Pós-graduação em Odontologia Restauradora - ICT

Caracterização química e estudo térmico de lodos provenientes da estação de tratamento de esgotos de Ribeirão Preto, SP, Brasil

Pós-graduação em Química - IQ

A incorreção política do policiamento da metalinguagem: a propósito do cultivo irracional do politicamente correto em linguagem

O artigo avalia o descompasso entre a liberdade de expressão, que no Brasil é garantida por lei, e ações culturalmente orientadas de patrulhamento do uso linguístico, que proíbem referências a características ou comportamentos de determinados grupos sociais, especialmente minorias. O descompasso se transforma em equívoco grave quando tal policiamento se estende às atividades de natureza metalinguística, que, baseadas na reflexão sobre os usos existentes, equacionam os significados das entidades do léxico ativado. O estudo examina especificamente a tarefa lexicográfica e avalia sua natureza e seu papel, dentro do universo de construção do saber sobre a língua.

Efeitos de diferentes sistemas de clareamento dental sobre a rugosidade e morfologia superficial do esmalte e de uma resina composta restauradora

The aim of this study was to evaluate and compare the roughness and superficial morphology of enamel and a composite restorative resin after different bleaching techniques application. Material and Methods: Bovine incisors were selected and standardized cavities were prepared on the buccal surface, which were restored with composite resin. The teeth were distributed according to the following treatments: G1- bleaching with 10% carbamide peroxide (CP); G2 - bleaching with 38% hydrogen peroxide (HP); and G3 - bleaching with 38% of HP associated to light irradiation. For G1, the bleaching gel was applied for 8 hours daily during 21 days. For G2 and G3, 3 sessions were performed, consisting of 3 applications of 15 minutes each, with 7 days of intervals between the sessions. For G3, the LED (470nm) light was used to activate the bleaching agent for 6 minutes. The surface of enamel and composite resin were evaluated before and after the bleaching procedures using a roughness tester and an atomic force microscope. Results: The results showed significant differences in surface roughness of enamel after bleaching only for G1 (Wilcoxon, p<0.05). For composite resin, neither group showed a statistical difference compared to control (Mann-Whitney, p>0.05). Conclusion: It was concluded that the increase in the roughness of enamel occurred only after bleaching therapy using a gel with 10% of CP. The bleaching procedures evaluated in this investigation did not increase the roughness or cause changes in the superficial morphology of the composite resin.