459 resultados para Descrição genética


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Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Ciências Biológicas (Zoologia) - IBB

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Pós-graduação em Agronomia (Agricultura) - FCA

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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A presente invenção refere-se a um método e kit para identificação genética humana por meio da análise de polimorfismos específicos do DNA mitocondrial para aplicação em populações miscigenadas como a brasileira, por exemplo. A técnica desenvolvida, além de permitir a identificação do indivíduo, permite també classificá-lo em halogrupos do DNA mitocondrial possibilitando a identificação da origem ancestral materna do indivíduo testado. A referida invenção pode ser aplicada na área de genética forense, pela polícia científica ou por laboratórios particulares, tendo como principais beneficiados populações miscigenadas que não dispõem de técnicas específicas para sua identificação e classificação genética.

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The present invention describes a method for transforming chemolithotrophic acidophilic bacteria using electroporation technology. The proposed method allows transforming a bacterial line using a transformation vector, the pAF vector, which contains an origin of vegetative replication that allows the vector to replicate inside the bacteria without altering the natural physiological functions of the latter. Also disclosed is the use of the bacteria modified according to the invention in bioleaching processes of sulphated copper, gold, uranium, nickel, zinc and cobalt ore, inter alia.

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This study aimed to evaluate the genetic diversity and relatedness of 24 accessions of Theobroma grandiflorum, originating from three units of Embrapa, aiming their use as parents in hybridization specie programs. The genetic markers used were heterologous microsatellite loci developed for cocoa. In the population studied 45 alleles were found. The effective average number of alleles per locus (2.33) was less than the average number of alleles per locus (3.21), indicating that many alleles have low frequency. The observed heterozygosity at polymorphic loci ranged from 0.33 to 1.00 with a mean of 0.54 and expected heterozygosity ranged from 0.48 to 0.76 with a mean of 0.54. The fixation index medium between loci (0.003) was not significantly different from zero. The estimate of relatedness between pairs of individuals indicates that some may be relatives, including half-brothers and clones. The results suggest that the accesses of T. grandiflorum analyzed contain a moderate level of genetic diversity and absence of inbreeding and therefore great potential for use in breeding programs.

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The Cerdocyon thous is a canid that has a wide distribution in South America and, besides some general aspects, its morphology is little known in the literature, especially regarding the nervous system. With the aim of elucidating the anatomical composition of brachial plexus, we studied three male specimens from Paragominas-PA, donated to the Morphological Laboratory of Animal Research (LaPMA), Federal Rural University of Amazonia (UFRA), after death by trampling. The animals were fixed in an aqueous solution of 10% formaldehyde for bilateral dissection of the origin of the brachial plexus. The brachial plexus of C. thous is derived from the last three cervical nerves and the first thoracic nerve (C6-T1). The main nerves that compose it, with their respective origins were the suprascapular nerve, subscapular nerve and musculocutaneous nerve (C6-C7), axillary nerve (C7-C8), radial nerve (C7-T1 and C7-C8), median nerve, ulnar nerve, thoracodorsal and thoracic lateral nerve (C8-T1). We conclude that the brachial plexus of C. thous is similar to that described for the domestic dogs, showing small differences in the composition of some nerves.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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This study has investigated the genetic variation for silvicultural traits in an open-pollinated progeny test of Astronium graveolens Jacq., established at Luiz Antônio Experimental Station (State of São Paulo, Brazil). The trial was planted in a random block experimental design, containing 23 families, six replications and five plants per plot. The traits measured were diameter at breast height (DBH), total height and stem form. The assessments were taken at the age of 19 years. Significant differences were not detected by the analysis of variance, suggesting that the genetic variation was low, as well as the probability to raising genetic gains through selection among progenies. The coefficient of genetic variation was moderate for the traits height (8.2%) and DBH (21.2%) and low to stem form (4.0%). However, the average coefficient of heritability among progenies was low for all studied traits (ranging from 0.02 to 0.15), confirming the low probability of genetic improvement of this population by selection among progenies.

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The study of the nervous system is increasing in Veterinary Medicine. Transcranial ultrasonography (TCUS) has the advantage of being a non-invasive and low cost method compared to computerized tomography (CT) and Magnetic Resonance Imaging (MRI). The temporal bone has been used as an acoustic window in TCUS in humans. This study aimed to correlate transcranial ultrasonographic images obtained through the temporal and occipital window with healthy dog's encephalic anatomy, and to standardize the technique. 37 adult mongrel dogs were used: 30 animals in vivo, in order to perform USTC screening and seven dog corpses for brain section as well as USTC planes. Data analysis was accomplished by the non parametric Wilcoxon test. Results obtained indicate that TCUS (in dorsal and oblique planes) is a viable method for brain evaluation in dogs weighting up to 10kg without anesthesia.

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The objectives of this study were to describe the potential for nitrogen retention by two techniques: comparative slaughter and nitrogen balance; compare the adjusted models for each technique and estimate the maintenance requirement for pullets. Assays were performed during 14 to 28, 56-70 and 98 to 112 days of age, using 168 pullets. The design was completely randomized with seven treatments and eight replications. Treatments consisted of protein levels ranging from 75 to 435gkg-1 of dry matter. The variables were collected by comparative slaughter and nitrogen ingested and deposited in nitrogen balance technique intake and nitrogen excretion. The intercept of the exponential relationship between ingested and excreted nitrogen was considered a requirement for maintenance. The adjusted models were compared by using the maximum likelihood ratio. The techniques described differently compared the requirement for maintenance of nitrogen. For comparative slaughter requiring maintenance was estimated at 342, 372 and 543mg/kgPC0,67 and for nitrogen balance was 342, 225 and 284mg/kgPC0,67 for the period of 14 to 28, 56-70 and 98 to 112 days of age, respectively.

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Functional grammar currently has a great acceptance in linguistics, mainly because it can enlighten grammatical facts' motivation in the structure of a text. From its emergence, the tradition of studying grammar by grammar has come to an end. Demonstrative pronouns, for instance, have begun to be viewed as efficient tools of text cohesion, used to resume terms from previous clauses. This task, however, ends up leaving an endless trail of black boxes. How is it possible to explain the origin of demonstratives' anaphoric functioning if they are originally used to indicate things or people relative to the interlocutors' spatial position? This work aims at showing that Cognitive Linguistics arises just as an option for opening those black boxes. This article focuses on one of its themes - the conceptual blending theory - to support this possibility. Firstly, it was necessary to integrate the cognitive model into the complexity theory, according to Bybee (2010) and Castilho (2009), who understand language as a complex adaptive system. After that, a brief updated description on the conceptual blending theory is made and its application in some grammatical facts of the Brazilian Portuguese language is suggested under the morphological and syntactic levels.