Ultrassonografia modo B de alta resolução modo Doppler e uso de contraste de microbolhas na avaliação testicular de gatos domésticos

Pós-graduação em Medicina Veterinária - FCAV

Experimental model of intestinal endometriosis: pilot study

Aims: The objective of this study is to create an experimental model of intestinal endometriosis in pigs, which might allow better understanding of deep infiltrating endometriosis and development of new treatment techniques. As secondary objective, we intend to create endometrial implants accessible by transrectal ultrasonography (TRUS). Study Design: Surgical experimental study in swine. Place and Duration of Study: This study was performed at the Instituto de Ensino e Pesquisa do Hospital Sírio-Libanês, São Paulo, Brazil, between January 2012 and December 2012. Methodology: Two sexually mature female minipigBR pigs underwent two laparotomies (each animal). The first laparotomy was performed to implant two fragments of autologous endometrium in the rectal wall. The second one was performed thirty days later to visualize, measure and obtain tissue of the site of the implants for histopathology study. A TRUS study was performed prior to the second surgery. The Institution’s Animal Utilization Study Committee approved the study. Results: In the first laparotomy a 5-cm segment of right uterine horn was resected. The endometrium was separated from the myometrium through sub-endometrial saline injection. Two endometrial fragments (1.0 x 2.0 cm) were dissected and sutured in the intra peritoneal anterior rectal wall of the animals. Thirty days later, all implants were identified during preoperative TRUS. “En-bloc” resection of the intestinal segment with the implants was performed during the second surgery. The autologous implants of endometrium invaded the muscular layer in one of the two animals. Conclusion: We demonstrated that the creation of an animal model of deep infiltrating endometriosis with intestinal involvement is feasible through a simple surgical technique. We believe that this model can be applied in experimental and clinical studies but further studies are necessary to refine the technique.

Evaluation of clinical, ultrasonographic and hysteroscopic parameters in the differentiation of endometrial polyps and cancer of endometrium

Study Objective: To evaluate the diagnostic accuracy of transvaginal ultrasound and office hysteroscopy in the differentiation between endometrial polyps and endometrial adenocarcinoma. Design: This is a prospective 100 women longitudinal study, 24 to 80 years, submitted to hysteroscopic polypectomy (n = 80) or surgery due to endometrial adenocarcinoma (n = 20), from january 2010 to December 2011. Clinical, ultrasonographic and hysteroscopic parameters were analyzed and compared with histopathologic find. Statistical analysis were performed utilizing the Tukey, Kruskal-Wallis, Dunn and Mann-Whitney test, with a confidence interval of 95% and p\0, 05 statiscally significant. Setting: Botucatu Medical School. Intervention: Prospective analysis of clinical, ultrasonographic and hysteroscopic parameters in patients with diagnosis suspected of endometrial polyps and adenocarcinoma of endometrium were performed. According to the diagnosis, hysteroscopic polypectomy or pan hysterectomy with lymph node sampling was realized. After the surgery and histopathological study, statistical analysis of parameters was performed and the results were compared between groups. It was Research Ethics Committee approved. Measurements and Main Results: There were no differences between age, BMI, menopause, TH use and associated diseases among groups. The main symptom of endometrial cancer was the postmenopausal bleeding, affecting 84,2% of women against 34,8% of polypectomy group. The majority of women with endometrial polyps were asymptomatic. Transvaginal ultrasonography showed no ability to differentiate cases of endometrial cancer compared with the cases of endometrial polyps, considering the presence of endometrial thickness and blood flow on color Doppler. Office hysteroscopy showed significant changes in 75% of the adenocarcinoma cases, especially the presence of diffuse hypervascularity with atypical vessels. Conclusion: Still remains an inability to establish clinical parameters and reliable ultrasound imaging to differentiate endometrial polyps and cancer of endometrium. Attention should be given to hysteroscopic exams presenting diffuse endometrial hypervascularization with architectural distortion of the vessels. The recommendation of our service remains the systematic removal of all endometrial polyps.

Diagnóstico por imagem nas enfermidades tireoidianas em cães: revisão

The diagnosis of various diseases has become more accessible and accurate with the rapid development of imaging modalities aiming to assist in medical diagnosis, and thereby in veterinary medicine. Different diagnostic imaging modalities such as ultrasonography, computed tomography, magnetic resonance imaging and scintigraphy can be performed to obtain information about thyroid diseases, each one with advantages and disadvantages, depending on the thyroid disease in question. Diagnostic imaging is a tool that not only assists in the diagnosis, but also helps treatment and assessment of prognosis of thyroid diseases. The aim of this article is to discuss the limitations and benefits of each diagnostic imaging modality available in the veterinary medical field, and also to present the newest diagnostic imaging modalities in order to maximize and make more accurate diagnosis of thyroid diseases.

Avaliação ultrassonográfica abdominal modo-B em cães e gatos filhotes: revisão de literatura

In veterinary medicine, the same clinician usually follows an animal from birth to old age, whereas in human medicine different specialists are required. It is therefore essential that veterinarians know the normal imaging, biochemical, hematological and physical exam values for younglings, which differ significantly from those of adults of the same species. Abdominal radiographies of young animals are poorly defined due to differences in tissue constitution in relation to adults. These same factors, however, substantially improve sonographic images of the region. Nonetheless, the interpretation of B-mode sonographic findings in puppies is difficult due to the lack of information about the normal parameters in this phase of life. The objective of this study was to compile information about peculiarities of abdominal B-mode ultrasonography in puppies and kittens.

Ultrassonografia transtorácica extracardíaca: revisão de Literatura

Radiography is usually the primary method of diagnosis to be used in the initial evaluation of the chest. In view of recent technological advances in ultrasound machines, noncardiac transthoracic ultrasound has become an important method of diagnosis for diseases of the lung, pleura, mediastinum and chest wall. Despite limitations due to the difficulty of efficient sound propagation in air, this exam allows fast decisions and makes sample collection safer and more effective. Knowledge of the sonographic chest anatomy is however restricted because of the artifact caused by the presence of air in lungs. When absent, it facilitates the detection of thoracic alterations. The aim of this review is to present the main abnormalities that are likely to be detected by ultrasonography.

Orquiepididimite causada por Corynebacterium sp e Proteus mirabilis em cordeiro da raça Lacaune: relato de caso

Orchiepididymitis is the inflammation of both, testis and epididymis and may be caused by microbial agents such as Brucella ovis. Thus, the aim of the present report is to present a case of orchiepididymitis in a Lacaune ram with unilateral testicular enlargement. Ultrasonography of the testis revealed the presence of a circular hypoechogenic structure adjacent to the testicular parenchyma. Corynebacterium sp was isolated from the surgically removed testis. Two months after removal of the affected testis, the animal returned to the Veterinary Hospital due to an enlargement of the remaining testis. A new ultrasonographic exam revealed the presence of abscesses in the testicular parenchyma and periorchitis. After orchiectomy, Proteus mirabilis was isolated from the material.

Diâmetro folicular e taxa de prenhez em protocolo de sincronização com GnRH ou Benzoato de Estradiol no dia 0 em vacas de corte

This work aimed to evaluate the pregnancy rate and follicular diameter using EB or GnRH on the insertion of progesterone implant (D0) in lactating beef cows. Two groups were tested in two experiments. In Exp. 1 were used 61 Nelore cows divided into two groups: G-BE (n = 32) and G-GnRH (n = 29), on D0 was inserted P4 implant (CIDR ) and applied 2 mL of BE (G-BE) or 2.5 mL GnRH (G-GnRH). In D9 was performed ultrasonography (U.S.) to measure the diameter of the dominant follicle (DF) present in the ovary and the implant was removed, with concomitant administration of 2.5 mL of PGF2a and estradiol cypionate (ECP ) followed by calves removal. After 48 hours all the cows were inseminated and the calves returned. In Exp. 2 50 cows were used following the same protocol described above, but the pregnancy was assessed without performing ovarian US. There was no difference (p>0.05) in pregnancy rate between treatments, BE (55%) or GnRH (41%), but the follicular diameter was significantly higher (p<0.05) in pregnant cows treated with EB (10.7 mm vs. 8.5 mm) and in cows treated with GnRH there was no difference (p>0.05) between pregnant and no pregnant cows (11.6 mm vs. 10.2 mm). We concluded the use of GnRH on D0 did not improve the pregnancy rate in lactating beef cows and follicular diameter was greater (p <0.05) in pregnant cows compared to non-pregnant only in G-BE.

Detecção imunoistoquímica de receptores de estrógeno e progesterona no endométrio de vacas Nelore (Bos taurus indicus) durante o anestro pós-parto

Twenty-four postpartum anestrous Nelore purebred cows were used in the study. Anestrous was determined based on the reproductive history which was confirmed in each cow based on plasma progesterone concentration and by transrectal ultrasonography. Endometrial biopsies were collected. The animals were separated into two groups according to maximum follicular diameter- Group 1: follicles <6mm (n=12) and Group 2: follicles >-6mm follicles (n=12). The immunohistochemistry technique was employed to evaluate the presence of estrogen and progesterone receptors in the uterine glandular epithelium and stroma. High counts of positive nuclei and high intensity of immunostain for estrogen and progesterone receptors in the glandular epithelium and stroma were observed in the two groups. However, the immunostain intensity of progesterone receptors in the glandular epithelium was higher in Group 2 compared to Group 1. When glandular epithelium and stroma were compared within each group, the relative number of estrogen receptors in the Group 1 was higher in the glandular epithelium compared to stroma and the immunostain intensity for the progesterone receptor in Group 2 was higher in the glandular epithelium compared to stroma. The results suggest that the mechanisms that control the expression of endomerial receptors in the anestrus are similar to the ones observed during the estrus cycle.

Casuística dos exames de diagnóstico por imagem na medicina de animais selvagens - 2009 a 2010

The aim of this study was to retrospectively evaluate in the casuistry on class and systems frequently diagnosed in wild animals that were sent to the FMVZ–UNESP-Botucatu diagnostic imaging service. The class of birds was the most referred to the centers, followed by the mammals and reptiles. The majority of the requested tests were the radiographs and in a minor scale the tomography and ultrasound. Although the birds were the greatest number of animals sent to the service, mammals were the most radiographed for wound control. The most frequently observed system was the musculoskeletal caused by trauma, especially in birds. The radiograph was the most exam initially indicated, then the animal could be forwarded to other imaging modalities, which in wild animals medicine is still limited to research.

Produção de oócitos e embriões bubalinos: efeitos da época do ano e da adição de óleo essencial de Lippia origanoides na maturação in vitro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Doppervelocimetria da artéria oftálmica em gestantes pré-eclâmpticas com recém-nascidos portadores de restrição de crescimento

Pós-graduação em Ginecologia, Obstetrícia e Mastologia - FMB