Natural latex films as carrier for casearia sylvestris swartz extract associated with ciprofloxacin

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Evaluation of allelopathic potential of leaf extract of kielmeyera coriacea on lactuca sativa l

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Phytotoxicity of Solanum aculeatissimum Jacq: leaves extract

The objective of this study was to evaluate the phytotoxicity of Solanum aculeatissimum Jacq. leaves ethanolic extract in seeds germination, development and fixation of Lactuca sativa seedlings. The same study also aimed to assess the mitotic index of lettuce roots meristematic cells, quantification of phenols and total flavonoids and triage by mean of phytochemical testing of the main secondary metabolites classes. Bioassays of germination, development of root and hypocotyl were carried out in Petri dishes using achenes of Lactuca sativa L. cv. 'Grand Rapids' (lettuce). Concomitantly, were evaluated the physico-chemical characteristics (pH, osmotic potential and electrical conductivity), mitotic index, quantification of total phenols and flavonoids and determination of phytochemical profile of the treatments extract. The results obtained in the bioassays demonstrate that the ethanol extract of S. aculeatissimuma presents phytotoxic potential in the development of lettuce seedlings, given that the concentration of 20 mg/ml showed greater inhibition (41% of germination). The extract contains significant amounts of antioxidants, total flavonoid and phenols, where the concentration 1000µg/mL showed higher values (86.50%). Furthermore, it was possible to observe the presence of compounds with allelopathic activity in the phytochemical screening test as coumarins, tannins, terpenes, flavonoids and alkaloids. Given the above it is clear that the ethanolic extract of S. aculeatissimum presents allelopathic substances with phytotoxic activity that can affect the germination and development of other plant species in their natural environment.

Sementes de piper cubeba nas dietas de alta e baixa digestibilidade para frangos de corte, uma alternativa de aditivo fitogênico

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Estudo de propriedades da resina acrílica termoativada por energia de micro-ondas frente às variações nos procedimentos de inclusão

On heat cured acrylic resins by microwaves energy the action of electromagnetic waves on methylmethacrylate promote the polymerization process. However, part of this energy is absorbed by the gypsum present in the flask and the function of this gypsum is to maintain the characteristics of specimen, nevertheless is unknown in the literature as the amount of water present in this gypsum affects the characteristics of acrylic resin. The proposal of this study was verified if dried gypsum influenced in some properties of the acrylic resins thermally activated by microwaves: presence of porosity, microhardness, roughness, flexural strength and dimensional change. Two different types of acrylic resin for complete denture were utilized: Lucitone 550 e Vipi-Wave and groups were formed: Lucitone 550, polymerization by microwave energy without gypsum treatment; Lucitone 550, polymerization by microwave energy with pretreatment gypsum; Vipi-Wave by microwave energy without gypsum treatment; Vipi Wave, polymerization by microwave energy with pretreatment gypsum; Lucitone 550 control group polymerization in hot water bath. For both situations were performed analysis of microhardness and porosity three different metallic shaped specimens were formed with followed dimensions: 2,0x2,0x2,0cm (8cm3); 2,0x2,0x1,0 (4cm3) e 2,0x2,0x0,5 (2cm3). Standardized wax specimens was used to prepare groups (n=9) and included in glass fiber flasks. The gypsum dry was made by microwave oven cycle (10min and 600W) and then the flasks were maintained in dry oven during two hours. Polymerizations of specimens were made and then polished. Wax patterns #7 obtained from the metal were embedded in plastic flask and following the processes of pressing and polymerization of the resin according to established groups. To evaluate the flexural strength, specimens 65mm x 10mm x 3.0mm from metallic patterns in bar format were made. ...

In vivo evaluation of the genetic toxicity of Rubus niveus Thunb. (Rosaceae) extract and initial screening of its potential chemoprevention against doxorubicin-induced DNA damage

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Green tea extract and its major constituent epigallocatechin-3-gallate inhibit growth and halitosis-related properties of Solobacterium moorei

Background: Solobacterium moorei is a volatile sulfide compound (VSC)-producing Gram-positive anaerobic bacterium that has been associated with halitosis. The aim of this study was to investigate the effects of green tea extract and its major constituent epigallocatechin-3-gallate (EGCG) on growth and severalhalitosis-related properties of S. moorei.Methods: A microplate dilution assay was used to determine the antibacterial activity of green tea extract and EGCG against S. moorei. Their effects on bacterial cell membrane integrity were investigated by transmission electron microscopy and a fluorescence-based permeability assay. Biofilm formation was quantified by crystal violet staining. Adhesion of FITC-labeled S. moorei to oral epithelial cells was monitored by fluorometry. The modulation of beta-galactosidase gene expression in S. moorei was evaluated by quantitative RT-PCR.Results: The green tea extract as well as EGCG inhibited the growth of S. moorei, with MIC values of 500 and 250 mu g/ml, respectively. Transmission electron microscopy analysis and a permeabilization assay brought evidence that the bacterial cell membrane was the target of green tea polyphenols. Regarding the effects of green tea polyphenols on the S. moorei colonization properties, it was found that biofilm formation on EGCG-treated surfaces was significantly affected, and that green tea extract and EGCG can cause the eradication of pre-formed S. moorei biofilms. Moreover, both the green tea extract and EGCG were found to reduce the adherence of S. moorei to oral epithelial cells. The beta-galactosidase activity of S. moorei, which plays a key role in VSC production, was dose-dependently inhibited by green tea polyphenols. In addition, EGCG at 1/2 MIC significantly decreased the beta-galactosidase gene expression.Conclusion: Our study brought evidence to support that green tea polyphenols possess a number of properties that may contribute to reduce S. moorei-related halitosis. Therefore, these natural compounds may be of interest to be used to supplement oral healthcare products.

Antiulcerogenic activity and toxicity of bauhinia holophylla hydroalcoholic extract

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Cytotoxic and genotoxic effects of ethanolic extract of Euphorbia hyssoptfolia L. on HepG2 cells

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influence of alternative drying aids on water sorption of spray dried mango mix powders: a thermodynamic approach

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Enhancement of quality and stability of dried papaya by pectin-based coatings as air-drying pretreatment

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effects of edible coatings on convective drying and characteristics of the dried pineapple

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)