Diversidade gênica no gênero Astyanax (Teleostei : Characidae) através da análise de PCR-RFLP de DNA mitocondrial

O gênero Astyanax é um dos mais abundantes e diversificados da família Characidae (subfamília Tetragonopterinae), com mais de 100 espécies nominais, estando amplamente distribuído na bacia do Alto rio Paraná. Esse gênero é caracterizado pela similaridade quanto à forma do corpo, além da alta variabilidade citogenética intra e interpopulações, sendo comum a ocorrência de espécies crípticas ou complexos de espécies. Devido à escassez de dados sobre genética molecular referentes a esse gênero, e a dificuldade na identificação taxonômica, fazse necessário um estudo utilizando marcadores moleculares que visem desenvolver métodos rápidos e eficientes para caracterização de espécies de Astyanax com base em análises de DNA. Em vista dessa necessidade, o presente trabalho teve como objetivo avaliar a eficiência da técnica de PCR-RFLP do gene mitocondrial Citocromo b na identificação e caracterização da variabilidade genética de cinco espécies do gênero Astyanax que ocorrem na bacia do Alto rio Paraná. O mtDNA das espécies alvo foi totalmente amplificado, num total de cerca de 1140 pares de bases (pb). As análises foram obtidas através dos haplótipos gerados com a digestão por três enzimas de restrição que clivam estes genes, sendo estas ALUI, BAMHI, e HPAII. Foram analisadas 2 populações de Astyanax paranae, A. altiparanae, A. fasciatus, A. bockmanni, e uma população de A. biotae, com amostragens variando entre 5 e 10 indivíduos de cada população. Como grupo externo foram analisadas duas populações de Astyanax ribeirae da bacia hidrográfica do rio Ribeira de Iguape. Ao final do trabalho foi possível a identificação de 4 das 6 espécies analisadas, sendo que as espécies mais divergentes são A. altiparanae e A. ribeirae, as espécies A. paranae + A. bockmanni e A. fasciatus + A. ribeirae são as mais fortemente relacionadas...(Resumo completo, clicar acesso eletrônico abaixo)

Geotecnologias para a análise da bacia hidrográfica do Córrego Wenzel - Rio Claro/SP: proposta de gestão de recursos hídricos

The expansion of urban in medium-sized citie, increase the importance of action planning and management of natural resources, among which water is the most important. Such action should include the integration of physical and man-made elements involved. In this context, it becomesimportant for watershed analysis and the use of geotecnologies. Thus, the objective was to demonstrate the contribution of the geo, as a tool for managing a river basin, allowing the integration of physical data and the analysis of anthropogenic environmental scenarios in urban areas. The area chosen, the basin of the Wenzel, is located in Rio Claro / SP, a city whose urbanization process has been intense and continuous. Thematic maps were generated from the physical environment: soil, slope and geological, as well as maps of land use. With regard to land use maps, these were drawn from the visual interpretation of material aerophotogrammetric, considering the years 1995 and 2010, performing an evolutionary analysis. It was observed that the area presents small variation in the physical and structural aspects, with lithological and geomorphological formation favoring the occurrence of floods, erosion and siltation. These processes are accelerated by the forms of land use, with intense urbanization during the period between 1995 and 2010, and poor preservation of permanent preservation areasand. In the floodplain areas there is a high waste disposal is the most notable environmental problem

A conformidade do projeto da usina hidrelétrica de Tijuco Alto em relação às áreas prioritárias para a conservação da diversidade biológica

A biodiversidade pode ser considerada como uma base da existência por proporcionar recursos e regular os processos naturais responsáveis pela manutenção da vida. Nas últimas décadas, a biodiversidade vem sendo perdida rapidamente, apresentando taxas de decréscimo nunca antes vistas, o que reduz a capacidade de aporte de recursos naturais para as gerações futuras. Em relação às estratégias de conservação da biodiversidade, os orgãos ambientais brasileiros têm empreendido diversos esforços na sistematização de dados e informações sobre biomas, ecossistemas e espécies ameaçadas com a finalidade do estabelecimento de indicadores e diretrizes para a conservação da diversidade biológica direcionados à tomada de decisões por parte de gestores públicos e privados, como aquelas que são deflagradas no âmbito dos processos de licenciamento ambiental. Dentre os instrumentos de gestão ambiental, o licencimaneto ambiental é aquele responsável pela autorização da implantação de empreendimentos potencialmente causadores de significativo impacto ambiental, como é o caso de usinas hidrelétricas. Para isso, o licenciamento ambiental deve estar baseado em estudos ambientais realizados a partir dos procedimentos metodológicos de avaliação de impacto ambiental, os quais devem preconizar os aspectos que caracterizam a vulnerabilidade ambiental da localidade geográfica onde está planejado o empreendimento. Neste sentido, o presente trabalho objetivou analisar de que forma os dados, informações, indicadores e diretrizes sobre a diversidade biológica brasileira, produzidos e estabelecidos pelo governo federal estão sendo utilizados no processo de licenciamento ambiental da usina hidrelétrica de Tijuco Alto do rio Ribeira de Iguape.

Variação inter-sexual na frequência de cromossomos Bs em Corydoras aeneus (Siluriformes: Callichthyidae)

The genus Corydoras, the highest among the Siluriformes, Callichthyidae is owned by the family, consisting of 177 valid species and widely distributed in cis-Andean portion of South America How striking feature has two longitudinal series of dermal plates covering almost the entire body. Cytogenetic studies in Callichthyidae show many chromosomal rearrangements, including events of polyploidy in their evolutionary history, particularly the genus Corydoras, in which the variation is the diploid number of 2n = 40 to 2n = 134 chromosomes. The absence of information on the frequency of chromosomes in the group Bs motivated this work with the species Corydoras aeneus. A population from the Tietê River basin in Ribeirão Claro (subbasin Corumbataí - Rio Claro, SP) was sampled a total of 20 subjects (10 males and 10 females) and 30 metaphases per individual were analyzed cytogenetically. Were carried out by impregnation techniques silver (Ag-NOR). The observed modal diploid number was 2n = 60 (26m +26 sm +8 st), with the variable occurrence of 1 or 2 Bs chromosomes in males and a B chromosome in females, both acrocentric. Regarding the variation in the frequency of chromosome Bs, the occurrence of two B chromosomes is directly linked to males, because there were no female sampled with the occurrence of two chromosomes Bs The low frequency of Bs in females suggests that this event can be sporadic this sex, different from males in which this appears to be set supernumerary chromosome showing a higher frequency of 2 Bs than the actual modal number of 2n = 60. Further studies will be performed to understand the dynamics of the B chromosome in the population

Simulação de produção de sedimentos em microbacias em função de cenários de restauração florestal

In São Paulo state, deforestation and agriculture activities are increasing soil losses processes, especially in areas of susceptible soils where properties not adopt soil conservation practices. Environmental adequacy at property level regarding Permanent Protection Areas and Legal Reserves is considered a factor on reducing soil losses and considering that we assessed soil losses of different scenario of environmental adequacy. Simulations of erosive process were carried out in 15 catchments of Corumbataí river basin, according to different forest restoration scenarios, as well as the current situation of land use/land cover. The scenarios include the implementation of Permanent Preservation Areas (PPA), the reforestation of variable source areas, and two scenarios, the Legal Reserve installation in 20% of each catchment, being one of them for most critical areas in terms of erosion and the other at random. It was observed that the establishment of PPA and the reforestation of hydrologically sensitive areas, offered only a small contribution to the control of the erosive process, resulting in a reduction of 10% and 7,8%, respectively, while the legal reserve in critical areas has a significant reduction of 69,8%. The random scenario, in turn, resulted in a reduction of only 21,4%. Results show that reforestation could reduce soil losses, but previous studies of land prioritization and planning could increase significantly its efficiency at this process.

Mapeamento citogenético de sequências histônicas e ribossomais revelam conservação e divergência cromossômica em populações de peixes da espécie Astyanax bockmanni (Teleostei, Characidae)

In the present work we performed molecular cytogenetic studies in two different populations of Astyanax bockmanni from streams of Botucatu, SP: one from Capivara river, on Tietê river basin and one from Água da Madalena river, on Paranapanema river basin. The results showed that the population of Astyanax bockmanni from Capivara river have a diploid number of 50 chromosomes, with karyotype consisting of 8 methacentric, 14 submethacentric, 12 subtelocentric and 16 acrocentric, while the individuals of the population from Água da Madalena river have 50 diploid chromosomes but with karyotype organized in 8 metacentric, 14 submetacentric, 16 subtelocentric and 12 acrocentric. Also, the rDNA 18s sequences are widely dispersed throughout the genome of two populations, with intra and interindividual variations. On the other hand, the sequences for rDNA 5S and Histone H1 remained chromosomally conserved in these two samples and sites located in pairs 2 and 19 (rDNA 5S) and the pairs 2 and 15 (Histone H1). The low dispersion of structural genes and a functional dynamic independence between sequences of rDNA 5S and rDNA 18S may be related to the process of karyotype maintenance and differentiation in these populations of Astyanax bockmanni

Contribuição das águas subterrâneas para a vazão do Ribeirão Boa Vista ou Córrego do Lajeado

This paper aimed to give a contribution to improve water resources management, consists of the analysis of interaction among groundwater and streamflow, by through the analysis of the relationship between basic and total streamflow, based on measured hidrological data from Ribeirão Boa Vista or Córrego Lajeado. watershed. Comparisons among groundwaterflow in this basin with another one in Rio Corumbataí basin were made based on the specific basic flow, in order to verify the possibility of the utilization of measured hydrological data in other basins with similar geological characteristics and soil use and occupation. Based upon the comparisons that were made, specific basic flow does not represent a good tool for extrapolation of measured data, indicating that this comparison shall be weighted by others characteristics as permeability and thickness of geological formations. Finally, it was possible to conclude that basic flow may be considered as an additional parameter for analysis of hydrological and hydrogeological characteristics of a basin and to provide subsidies to release legal licenses for water resources uses. related to surface water as well as for groundwater and may also be used as a tool for consolidation of integrated management of water resources, that is considered as a necessary and essential practice to avoid conflicts between users of water resources in a river basin.

Abordagem metalômica quantitativa de mercúrio em peixes da região amazônica

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Microscopic Evidence of the Opportunistic Reproductive Strategy and Early Sexual Maturation of the Small-Sized Characin Knodus moenkhausii (Characidae, Pisces)

We investigated the reproductive biology of Knodus moenkhausii, an abundantsmall-sized characin fish with broad occurrence in the Parana River basin, Bra-zil. Specimens were collected monthly to determine fecundity, length at firstmaturity, reproductive period and spawning type. Gonads were macroscopically classified according to their form, size and texture in three different stages(immature, maturing or mature). Histological procedures were conducted toconfirm gonadal developmental stages, and it was possible to notice thatmaturing females actually presented atretic oocytes, and all males that weremacroscopically classified as immature, maturing and mature actually presentedabundant spermatozoa in their gonads. Because of these discrepancies, a reclas-sification of gonadal maturations stages was needed after histological analysis,reinforcing its importance to studies on the reproduction of small characins.Reproduction occurred throughout the year though with two peaks. The lengthof the smallest mature individuals was 13 mm SL for males and 24 mm SL for females. Despite presenting relatively small batch fecundity, some life historytraits such as early reproduction, multiple spawning throughout the year, inassociation with known opportunistic feeding habits, explain the high abun-dance of this species in locations where it occurs.

Análise espaço-temporal do escoamento fluvial nas bacias hidrográficas dos rios Aguapeí e Peixe, Oeste Paulista, Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Geomorfologia e evolução quarternária da calha do Alto Rio Paraná, no segmento livre de barramentos, entre os reservatórios de Porto Primavera e Itaipu

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Políticas públicas e desenvolvimento Territorial: o Programa Territórios da Cidadania no Estado de São Paulo

Pós-graduação em Geografia - FCT

Variação espacial do grau de trofia e da biomassa fitoplanctônica no reservatório de Itupararanga (São Paulo, Brasil)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Geomorfologia e paleo-hidrologia dos megaleques dos rios Cuiabá e São Lourenço, quaternário da Bacia do Pantanal

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A competição interespecífica explica a distribuição espacial de macrófitas aquáticas em estuários?

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)