Composição da carne de frangos de corte alimentados com biomassa bacteriana

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aspectos da biologia reprodutiva da arara-azul Anodorhynchus hyacinthinus (Latham, 1790) no mosaico Carajás-PA

Pós-graduação em Ciências Biológicas (Zoologia) - IBB

Programação térmica fetal e duração da fase de eclosão: efeitos sobre a qualidade na eclosão, variáveis sanguíneas, preferência térmica e resposta ao desafio térmico de pintos fêmeas

Pós-graduação em Zootecnia - FCAV

Efficacy of prebiotics, probiotics, and synbiotics on laying hens and broilers challenged with salmonella enteritidis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Multiphase feeding program for broilers can replace traditional system

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Feeding high-moisture corn grain silage to broilers fed alternative diets and maintained at different environmental temperatures

The effects of the dietary substitution of dry corn by high-moisture corn grain silage (HMCGS) were evaluated on the performance, nutrient digestibility and serum biochemical parameters of broilers reared in an alternative production system and submitted to different environmental temperatures. A total of 288 one-day-old male Cobb chicks were distributed according to a randomized block design in a 3x4 factorial arrangement: three environmental temperatures (hot, thermoneutral or cold) and four levels of HMCGS in substitution of dry corn (0%, 20%, 40% or 60%). The acid analysis showed that the evaluated HMCGS contained average percentage values of ethanol, lactic acid, and acetic acid (expressed in 100% of dry matter) of 0.7690, 2.7320 and 0.0249%, respectively. Propionic and butyric acids were not detected. Dry corn and HMCGS presented pH values of 5.8 and 3.3, respectively. The inclusion of HMCGS reduced dietary pH, as shown by the values of 5.7, 5.4, 5.1 and 4.8 recorded for the diets containing 0%, 20%, 40% and 60% of HMCGS, respectively. There was no significant interaction between diets and environmental temperature. HMCGS may replace up to 40% dry corn in broiler diets when performance, triglyceride levels, and HDL-cholesterol ratio is considered, and up to 60% when nutrient digestibility is evaluated. High environmental temperature impairs broiler performance, nutrient digestibility, and serum biochemistry, demonstrating the influence of environmental temperature on broiler metabolism and performance.

Intestinal integrity and performance of broiler chickens fed a probiotic, a prebiotic, or an organic acid

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Salmonella serovars in laying hen flocks and commercial table eggs from a region of Sao Paulo State, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Gallus gallus domesticus are resistant to infection with Neospora caninum tachyzoites of the NC-1 strain

The aim of this study was to experimentally evaluate infection in Gallus gallus domesticus with Neospora caninum tachyzoites of the NC-1 strain. Experimental infection was conducted in 90-day-old chickens, embryonated eggs and bioassays in dogs. In the first experiment, poults were randomly divided into four groups. Groups I and II were provided feed with coccidiostat, whereas groups III and IV received feed without coccidiostat. When the poults from groups I and III reached 90 days of age, they received a subcutaneous inoculation of N. caninum. Once the hens entered their egg-laying period, during the following 30 days, the eggs were collected, identified, weighed and placed in an incubator. On the 70th day after inoculation, all animals, including the chicks, were euthanized. Tissue samples from the adult poultry and chicks were collected for histopathology, immunohistochemistry (IHC) and PCR. Brain tissue and pectoral muscle samples from infected birds were fed to two dogs. Notably, the average weight of the group III eggs was lower than that of the group IV eggs (p <0.05). No changes consistent with infection in adult poultry or chicks were detected by histopathology or IHC; moreover, no amplified parasite DNA was detected in the birds'tissues or dogs'feces. No dog eliminated oocysts. In the second experiment, the embryonated chicken eggs were inoculated with 1 x 10(2) N. caninum tachyzoites, on the 10th day of incubation, and chicks born from these eggs were housed in boxes suitable for the species and received commercial feed and distilled water ad libitum. On the 30th day after infection (DAI), the poultry were euthanized, and their organs were processed as described in experiment I. The amplification of parasite DNA was observed in the spleen and pectoral muscles of one of the birds. The ingestion of bird tissues by dogs did not result in oocyst elimination. These results indicate that the parasite may have been eliminated by the host and that the use of tachyzoites to induce chronic disease might be a poor source for hens. (C) 2014 Elsevier B.V. All rights reserved.

Traceability of poultry offal meal in broiler feeding using isotopic analysis (delta C-13 and delta N-15) of different tissues

Our goal was to trace the inclusion of poultry offal meal (OM) in diets by using carbon (13C/12C) and nitrogen (15N/14N) isotopic ratios of different tissues in order to contribute for the development of an independent technology for the certification of the feeding of broilers reared on diets with no addition of animal ingredients. Eighty one-day-old chicks were randomly distributed into five experimental treatments, that is, diets containing increasing levels of OM inclusion (0, 2, 4, 8 and 16% OM), with four replicates of four birds each. At 42 days of age, four birds per treatment (n=4) were randomly selected, weighed, and sacrificed to collect breast muscle (Pectoralis major), keel and tibia samples to determine their isotopic ratios (13C/12C e 15N/14N). It was observed that 13C and 15N enrichment increased as a function of increasing OM inclusion in all diets. The analyses of the Pectoralis major showed that that only treatments with 8 and 16% OM dietary inclusion were different form those in the control group (0% OM). on the other hand, when the keel and tibia were analyzed, in addition to 8 and 16% OM), the treatment with 4% OM inclusion was also different from the control group. The use of isotopic ratios of stable carbon and nitrogen isotopes is an alternative to trace OM inclusion in broiler diets as it is capable of tracing OM levels below those usually practiced by the poultry industry in Brazil.

Immunomodulatory activity and control of Salmonella Enteritidis colonization in the intestinal tract of chickens by Lactobacillus based probiotic

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Incubation temperature manipulation during fetal development reduces adiposity of broiler hatchlings

Broilers are known as an efficient source of lean meat. Genetic selection resulted in broiler strains with large body size and fast growth, but a concomitant increase in fat deposition also occurred. Other than reducing nutrient intake, there is a lack of alternative methods to control body fat composition of broilers. The present study assessed whether incubation temperature (machine temperatures: 36ºC, 37.5ºC, and 39ºC; eggshell temperatures: 37.4 ± 0.08°C, 37.8 ± 0.15ºC, and 38.8 ± 0.33°C, respectively.) from d 13 affects broiler hatchling fat deposition. We analyzed adipocyte hypertrophy and proliferation in 3 body regions; weight and chemical composition of yolk-free chicks and yolk sacs; and serum lipid profile. Increased incubation temperature reduced abdominal and cervical adipocyte size. Independently of temperature, cervical adipocytes were smaller and showed higher proliferation than adipocytes in the abdominal and thigh regions. Smaller cervical adipocytes were observed in birds from eggs incubated at 36ºC and 39ºC. With regard to weight and composition of chicks, ash content as a percentage of dry matter was the only variable affected by temperature; it was higher in chicks from eggs incubated at 36ºC than at 39ºC and showed no significant difference between chicks incubated at 39ºC and 37.5ºC. Absolute and relative weights of yolk sacs were higher from eggs incubated at 39ºC than at 36ºC, and these two treatments did not differ from the 37.5ºC control. Absolute measures of yolk sac lipids, moisture, dry matter, and crude protein content were lower in chicks from eggs incubated at 36ºC, and no significant differences were found for these variables between chicks from eggs incubated at 37.5ºC and 39ºC. Hatchlings from eggs incubated at 36°C had significantly higher cholesterol levels than chicks incubated at the other 2 temperatures, but no additional effects on blood lipids were detected. Incubation temperature manipulation during fetal development altered cervical and abdominal adipocyte size in broiler hatchlings and could become a tool in hatcheries to manipulate chick quality, although further studies are needed to evaluate its long-term effects.

Turnover tecidual, desempenho e rendimento de carcaça em frangos de corte submetidos a diferentes níveis de metionina digestível

Pós-graduação em Zootecnia - FMVZ

Diet formulation techniques and lysine requirements of 1-to 22-day-old broilers

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Strategic use of dietary electrolyte balance and ratio for broiler chickens

The literature reports dealing with the dietary electrolyte ratio (K+Cl)/ Na are rare, although the concept has been proposed by Mongin in 1981. Thus, its application appears to be limited as a nutritional strategy in feed formulation, which usually meets only the minimum nutritional recommendations in Na, K and Cl. The objective of this study was to evaluate the performance of broilers submitted to different dietary electrolyte balances (DEB) Na+K-Cl and dietary electrolyte ratio (DER) from 1 to 21 d of age. A total of 1575 male 1-d old broiler chicks were randomly assigned to 5 treatments with 9 replicates of 35 chicks each. The treatments consisted of diets with 5 relation of electrolytes, arising from combinations DEB/DER 150/3, 250/2, 250/3, 250/4 and 350/3, with use of NaCl, NaHCO3, KCl, K2SO4 and CaCl2. All diets were corn-soybean meal based and formulated to meet or exceed the NRC (1994) requirements. Chicks had ad libitum access to feed and water in floor pens with wood shavings as litter. Body weight, feed intake and feed conversion ratio were measured at 21 d of age. It was found that only the feed conversion was significantly affected (P = 0.0142) by the combinations of relation of electrolytes (DEB and DER). The supplementation levels in DEB and DER were made to fit the data into a surface analysis to allow increasing levels of DEB (150–350 mEq/kg) and narrow and broad ratio of DER (2–4). For the canonical analysis of response surface was obtained the stationary point for body weight (DEB = 255.77 mEq/kg, and DER = 2.73:1) the value was 942.02 g; for feed intake (DEB = 251.69 mEq / kg and DER = 3.51:1), the value was 1200.02 g; and for feed conversion (DEB = 254.62 mEq/kg and DER = 3.06:1) the value was 1.35. The results of this experiment indicated that the best performance was obtained with combinations of relation of electrolytes for DEB between 251 and 255 mEq/kg and DER between 2.73:1 and 3.5:1.