Mensuração do comprimento das fibras para a determinação da madeira juvenil em Eucalyptus citriodora

The juvenile wood has peculiar characteristics from the anatomical structure and physical-mechanical properties, considering these aspects the knowledge of the wood is essential for wood adequate utilization. The aim of this work was to determinate the zone of juvenile wood of pith-bark direction in Eucalyptus citriodora. The juvenile and mature wood zones were determined across fiber length measurement in various height in the tree stem. Results showed that juvenile wood zone occurs approximately up to the 45 at 55 mm from the pith.

Determinação precoce do sexo fetal pela análise do DNA no plasma materno

Purpose: to verify the viability of early diagnosis of fetal gender in maternal plasma by the real-time polymerase chain reaction (real-time PCR) starting at the 5th week of pregnancy. Methods: peripheral blood was collected from pregnant women with single fetus starting at the 5th week of gestation. After centrifugation, 0.4 mL plasma was separated for fetal DNA extraction. The DNA was analyzed in duplicate by real-time PCR for two genomic regions, one of the Y chromosome and the other common to both sexes, through the TaqMan® method, which uses a pair of primers and a fluorescent probe. Patients who aborted were excluded. Results: a total of 79 determinations of fetal DNA in maternal plasma were performed in 52 pregnant women. The results of the determinations were compared to fetal gender after delivery. Accuracy according to gestational age was 92.6% (25 of 27 cases) at 5 weeks with 87% sensitivity, and 95.6% (22 of 23 cases) at 6 weeks with 92% sensitivity. Starting at the 7th week of pregnancy, accuracy was 100% (29 of 29 cases). Specificity was 100% regardless of gestational age. Conclusion: real-time PCR for the detection of fetal gender in maternal plasma starting at the 5th week of gestation has good sensitivity and excellent specificity. There was agreement of the results in 100% of the cases in which male gender was diagnosed, regardless of gestational age, and from the 7th week of gestation for female gender diagnosis.

Determinação da distribuição de um nível de turbidito na formação Macaé no campo de Namorado através de uso de atributos sísmicos

This study was developed on the basis of petrophysics characterization, 3D seismic interpretation, and rock-wire-log integration. The results were integrated with amplitude attribute through cross plot (rock attribute x amplitude value). 3D seismic data also allowed inteipreting the top of Lagoa Feia, Macaé, and Carapebus formations, and Outeiro Member. The analyses of the maps of structures and attributes, integrated with wire-log data, suggest that Namorado turbidite level is candidate to be mapped with stratigraphic attributes like AverageTroughAmplitude, RMS velocity and Total AbsoluteAmplitude. Lagoa Feia Formation attribute maps (Dip-Azimuth and TotalEnergy) allows to interpret a low fault density at the carbonate coquina level in the Namorado field. This level is also considered one at the best seismic reflector and an important reservoir of the Campos Basin.

Variabilidade clínica na determinação da dose tóxica oral em intoxicação experimental por fluoroacetato de sódio em gatos

The sodium monofluoroacetate (FAC) or compound 1080 is a potent rodenticide used for a rodents and vertebrate pest control. It was prohibited in many countries because of its high toxicity, but in Brazil exist evidences of ilegal use causing the intoxication in children and domestic animals. The fluoroacetate metabolite, fluorocitric acid, blocks body energy production by inhibit the Krebs cycle, resulting in neurological and cardiacs signs. In the present study, four group of oral toxic dosis of the FAC were compared in cats. The best oral toxic dose for clinical signs presentation, without cause acute lethality, was 0,45mg/kg. The clinical variability was dosis dependent and its intensity, in crescent order, was: light signs (dose 1: 0,3mg/kg), light to moderate (dose 2: 0,4mg/kg), moderate to severe (dose 3: 0,45mg/ kg) and severe (dose 4: 0,5mg/kg). There was individual clinical variability between animals that received the same oral toxic dose.

Determinação de desmineralização óssea em gatos após tirotoxicose experimental

Hyperthyroidism can result in serious effects on the bone metabolism in humans as well as animals. For a better characterization of thyrotoxicosis effects, 16 cats were induced into thyrotoxicosis by intaking a dose of 150 μg/kg of sodium L-thyroxine every 24 hours during 42 days. The hormonal levels were evaluated by radioimmunoassay technique and the bone mineral density of the right distal radius extremity was measured through the radiographic optical densitometry. Was verified significant bone demineralization seven days of hormonal intake as weel as radius demineralization.

Determinação de fármacos diuréticos em associação por cromatografia em camada delgada e espectrofotometria

A rapid, sensitive and reliable thin-layer chromatography/spectrophotometry screening procedure was developed for quantitative determination of diuretics associated in pharmaceutical dosage forms. The chromatographic method employed microcrystalline cellulose and butanol : acetic acid : water (4:1:1) or amilic alcohol : ammonium hydroxide 25% (9:1) as mobile phases and detection by U.V. light. The drugs were extracted using a simple procedure and were quantified by U.V. spectrophotometry. Results varied from 97.5 to 102.5% and are similar to those obtained by conventional methods. This method of quantification of diuretics is promising for quality control of drugs.

Efeito residual de calcário, nitrogênio e zinco na produção de matéria seca de Brachiaria decumbens em condições de campo

The balance of liming and fertilization practice with nitrogen and zinc could affect the production of the pasture. Thus, the present work has as objective to evaluate the residual effect of the application of calcareous rock, nitrogen, and zinc on the dry matter production of Brachiaria decumbens, during three cuts. For this, an experiment was installed in Selvíria, Brazil, on a distrofic red Latosol, from october 1997 to may 1998. The experimental design was randomized blocks, in a factorial project 3×3×2, with four repetitions. The treatments were three doses of nitrogen: 0, 50, and 100 kg/ha, three doses of lime: 0, 267, and 556 kg/ha, corresponding to 41, 50, and 60% of the levels of base saturation, respectively, and two doses of zinc 0 and 5 kg/ha, applied in the first year and reapplied in the second year, except for the limestone. Four cuts were done in the grass at 10 cm of height, with intervals of 49 days, to obtain the dry matter production. The limestone application in the first year did not provide to residual effect on the production of Brachiaria decumbens. However, the nitrogen fertilization increased the dry matter production of the grass. The application of zinc in the first year and the reapplication in the second year did not result in a productive response of B. decumbens. © 2009 Instituto Nacional de Investigaciones Agricolas INIA.

Determinação de cetamina em plasma por HPLC: Aplicação em um estudo de farmacocinética de associação medicamentosa em cães

A S(+) cetamina é um fármaco amplamente utilizado na medicina para induzir anestesia e a associação com midazolam é empregada para minimizar seus efeitos adversos. Associações medicamentosas podem resultar em interações farmacocinéticas e a disponibilidade de métodos bioanalíticos para a determinação da cetamina em plasma constitui ferramenta útil para a avaliação do perfil cinético do fármaco administrado isoladamente ou em associação. O presente estudo teve como objetivo o desenvolvimento e validação de um método analítico para determinação da cetamina em plasma por cromatografia líquida de alta eficiência (HPLC) e a investigação do perfil farmacocinético da cetamina em quatro cães hígidos da raça Beagle. A S(+) cetamina (10mg/kg) foi administrada pela veia cefálica em dose única isoladamente (protocolo I) ou associada ao midazolam (0.2mg/kg) (protocolo II) em estudo cruzado com intervalo de uma semana para washout. Amostras seriadas de sangue foram coletadas no intervalo de oito horas e analisadas por HPLC para a avaliação do perfil farmacocinético utilizando modelo bicompartimental. O método bioanalítico apresentou limites de confiança aceitáveis para sua aplicação em estudos de farmacocinética e os parâmetros área sob a curva (ASC0-8), volume de distribuição (Vd), clearance total (Clt), meia vida de eliminação (t/12 ß), constante de eliminação (ß), meia vida de distribuição (t1/2α) e constante de distribuição (α) não mostraram diferenças estatísticas significativas entre os grupos (p < 0.05, Wilcoxon). Os resultados obtidos sugerem que a redução dos efeitos colaterais da cetamina decorrente do uso da associação cetamina-midazolam não está relacionada a alterações no perfil farmacocinético da cetamina.

Efeito de métodos de determinação de parâmetros para o controle da irrigação na eficência do uso da água do feijoeiro, sob três diferentes lâminas de água no solo

The objective of this research was to study three methods of parameter determination for irrigation control under different tensions in common bean (Phaseolus vulgaris L.). The treatments were a combination of three methods of parameter determination for irrigation control (tensiometer, water retention curve, USWB Class A pan) with three soil water tensions (1:-30 kPa; 2:-60, kPa both for the whole plant cycle; 3:-60 kPa for the vegetative phase and -30 kPa for the reproductive phase). The experimental design was randomized blocks, factorial 32 with three replicates. Although no significant effect on water use efficiency was found, a tendency for increasing water use efficiency in treatments with tensions of -60 kPa at the vegetative phase and -30 kPa at the reproductive phase was observed in all methods of parameter determination for irrigation control. Water was saved as less irrigation was applied during the vegetative phase and more irrigation at the reproductive phase. No precipitation was observed from June to September 1995, leading to a high yield and therefore to a higher water use efficiency.

Comparação de métodos para determinação da estabilidade de agregados por vias seca e úmida em diferentes sistemas de uso e manejo do solo

The purpose of the work is to evaluate the stability by dry and humid of theaggregate in different systems or the use and management in Argissolos: agricultural, pasture and torest utilization. This work was realized in agricultural property, in Anhumas city, SR Several analyses including percentage of the aggregate (by way of dry and humid); the weighted mean diameter of the aggregate calculation and statistic analysis. Conclusion: the organic matter content of the A horizon of the soil under forest is 64 % more than the soil under pasture and 79 % more than the soil under annual crop; the WMDA of the aggregate obtained by dry and humid ways of the horizon A and its mean value for the soil decrease in the following sequence: PVAd - forest > PVe -pasture > PVd - annual culture, respectively, with the following values: 1.33560 and 1.445496 (D), 2.81114 and 2.351380 (H); 0.66748 and 1.011830 (D); 2.79642 and 1.624250 (H); 0.32468 and 0.993775 (S), 1.25808 and 0.983135 mm (H); the two methods are equally sensitive to reveal the effect of the soil use and management; the organic matter provides additional stability to the aggregates submitted to humid sieving and clay to the dry sieving: the WMDA obtained by dry and humid ways are statistically different for the soil profiles.

Resposta do manjericão (Ocimum basilicum L.) cultivado em solos corrigidos com calcário e escória silicatada: Caracterí sticas agronômicas e produção de matéria seca

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Determinação da resistência de Staphylococcus aureus: Um desafio?

The aim of this study was to identify the resistance profile of Staphylococcus aureus strains, in relation to induced clindamycin resistance, and to detect oxacillin resistance by the routine phenotypic methods. The strains were isolated from nasal or lingual swabs taken from healthy adult carriers with no medical history of hospitalization or antibiotic treatment. Eighteen strains were distinguished by the different patterns generated by pulsed field gel electrophoresis (PFGE). Four (22.2%) of these showed sensitivity to clindamycin by the conventional antibacterial susceptibility test, but demonstrated inducible resistance to it by the D-test. One strain (5.6%) was characterized as borderline oxacillin-resistant S. aureus (BORSA), and another (5.6%) as CA MRSA (community-associated methicillin-resistant Staphylococcus aureus). Both of these strains were shown to be cefoxitin susceptible by the disk diffusion test. The polymerase chain reaction (PCR) failed to detect the mecA gene in this last strain and it was thus classified as BORSA. These results show the importance of incorporating the D-test into the routine lab tests for S. aureus inducible clindamycin resistance and also of including the cefoxitin resistance test among the phenotypic methods for MRSA characterization.

Estudo de metodologia analítica para a determinação do cetoconazol em formulações farmacêuticas

Ketoconazole is a synthetic broad-spectrum oral and topical antifungal drug derived from imidazole, effective in the treatment of superficial mycoses and systemic infections. In this study we have tested several methods to analyze ketoconazole in various pharmaceutical products containing this drug, employing techniques such as UV and IR spectrophotometry and thermal analysis. The results showed that UV spectrophotometry is a fast, practical and economical method and indicated that other methods, such as IR spectrophotometry and thermal analysis, could be good alternative methods for ketoconazole analysis in certain pharmaceutical forms.

Geoprocessamento e sensoriamento remoto aplicado na determinação da aptidão agrícola de uma microbacia

Soil use for the development of activities as agriculture and livestock has been causing great alterations in the environment, mainly when these are practiced intensively, disrespecting the fragility and aptitude of the natural resources. Therefore, it is essential that the planning of the agricultural activities is done, taking into consideration the several environmental criteria involved in the decision-making process. Thus, this study aimed to analyze the agricultural aptitude classes of lands from a watershed through geographical information system (GIS). The Arroio Ajuricaba watershed is located in the Municipality of Marechal Cândido Rondon - PR among the coordinates UTM 787309m E and 793892m E; 7275026m N and 7281310m N, in the Spindle 21, presenting an area of 1681ha. Soil maps, in semi detail scale, was the digital cartographic base used. The results allowed to conclude that 42.41% of the basin presented a good aptitude for farming in handling level of high technology (handling level C), regular aptitude for B, and restricted aptitude for A [class 1(a)bC] and that 12% of the area had regular aptitude for farming in the three handling levels (class 1abc). The watershed lands (14.24%) presented regular aptitude for farming in handling level C, restricted aptitude for B, and inapt for A [class of aptitude 2(b)c]; 15.85% presented good aptitude for planted pasture (class 4P) and 12.21% were considered without aptitude for agricultural use (class 6). We can say that 71.94% of the lands has aptitude for farming, although they present different degrees of limitations that request different treatments for its conservation.

Análise de crescimento em clones de eucalipto submetidos a estresse hídrico

The aim of this study was to evaluate Eucaliptus grandis genotypes (Clones 105 and 433) in relation to drought tolerance, through growth plant analysis. Black PVC pots with 10 liter volume were used for cultivate plants in polyethilene greenhouse oriented east/west. Completely randonmized design with four treatments was used: two clones and two minimum soil water potentials (- 0.03 and -1,5 MPa) and sixteen replicates. Pots were weighed daily in order to evaluate water content and characteristic soli water curve was determined. Plant development was obtained each 15 days from planting until 60 days through total dry matter (DM), leaf area index (LAI), leaf area ratio (LAR), net assimilative ratio (NAR), specific leaf area (SLA), relative growth ratio (RGR) and absolute growth ratio (AGR). Results showed that clone 105 presented less sensibility to water deficit, which qualify it as genetic material for use under dry soil conditons. On the other hand, both clones had similar behavior with no water restrictions.