Influência do banho de aspersão ante-mortem na contaminação microbiana da carne bovina

To determine the effects of the pre-slaughter showering on some meat quality parameters, the bacterial changes in the Longus colli muscle and the contamination of the meat surface at three different points of the slaughter line were studied. Sixteen Nelore steers were slaughtered in a commercial slaughterhouse. Eight animals were submitted to pre-slaughter showering; a control group of eight animals were slaughtered without showering. Aseptic samples were collected for evaluations in the muscle depth, in the anterior portion of Longus colli muscle, just before chilling. The swab method was used for sampling carcass surface right after dressing, before carcasse washing, and at the beginning of chilling. Longus colli muscle samples were used to determine bacteria total count, psychrotrophic count and Enterobacteriaceae count, after 5, 24 and 48 hours from slaughtering, and in carcass surface, bacteria total count and psychrotrophic count. Multivariate methods were used to evaluate bacterial data the use of pre-slaughter showering did not affect the bacteria total counts, in the deep tissue. A significant growth of psychrotrophic bacteria was detected in both treatments. No significant differences (P>.05) were found in bacteria total count and psychrotrophic count between treatments. Also, no differences (P>.05) were detected between counts taken at different momments at the kill floor: skinning, before carcasse washing, and at the cooler, before chilling.

Efeitos dos danos mecânicos e fisiológicos na colheita e beneficiamento de sementes de soja

The objective of this study was to evaluate the effect of mechanical damage and physiological events in harvesting and processing of soybean cv. Mosoy RR 8000. The samples were taken during harvest manual, mechanical harvesting and during processing (receipt, pre-cleaning, cleaning, spiral separator, classification and gravity table). The physiological and physical quality was analized through the purity, germination, vigor (first germination count, seedling dry matter, accelerated aging, electrical conductivity, tetrazolium, mechanical damages and seedling field emergence) tests. The statistical design used was a entirely randomized with nine treatments (9 sampling points) with 4 replications, being the means compared by the Tuckey test at 5% probability. In the purity and seedling field emergence were observed highly significative difference between the sampling process, also this differences were obtained the first germination count, seedling dry weight matter, accelerated aging and electrical conductivity which showed smaller results for the mechanical harvesting when compared with the manual harvesting. The germination was obtained differences at 5% for the manual harvesting in relation to the mechanical harvesting were obtained smaller results, being the main cause of reducing the soybean seed quality, when compared with the manual harvesting.

Distribuição de poros e densidade de latossolos submetidos a diferentes sistemas de uso e manejo

Man cultivates the soil for centuries, but the intensive business and use of the soils under Cerrado vegetation for agricultural production grew out of the seventies. The objective of this study was to evaluate soil physical characteristics as a function of sampling time and the soil uses in a Cerrado area in Uberlandia City - MG, Brazil. The managements were adopted: degraded pasture (M-1), conventional tillage (M-2), minimum tillage (M-3), tillage absence (M-4), no-tillage (NT) for three years (M-5); NT for nine years (M-6), NT for three years after Pinus (M-7), PD for one year after Pinus (M-8) and Pinus forest (M-9) with 25 years old. The evaluations were conducted in 2002/03 growing season, in two areas. The soils were: area 1, an Oxisol (Red Latosol - LV-1, M-1 through M-5) and area 2, two Oxisols (Red Latosol and Red-Yellow Latosol - LVA and LV-2, M-6 through M-9). The physical attributes studied changed depending of the soil class, sampling time and management systems, with emphasis on the area 2 soils, which, in general, better preserved its main physical attributes. Managements with intense tillage, such as the M-2, are the most soil physically degrade, presenting mostly negative changes to soil bulk density, total porosity, microporosity and macroporosity. Since the systems which promote less tillage, in short term, to preserve desirable physical attributes. The M-9 system had the lowest attributes range, compared to the others.

Estudos hídricos com substratos vegetais para cultivo de orquídeas epífitas

Different growing media were compared as to water adsorption and water loss, at Jaboticabal, SP, Brazil, through a three-year period. The objective was to recommend substrates other than tree fern fiber for cultivation of epiphytic orchids. Two treatments of each substrate were used in each sampling: materials stored in laboratory (without use) and materials exposed to conditions of orchid cultivation under laths (used). Generally, the substrates without use adsorbed less water than used substrates. When materials without use were compared, the tree fern fiber retained initially the greatest quantity of water and the blocks of pressed coconut bark, the smallest. However, these blocks gained a great capacity of water adsorption after being used. Charcoal added to the growing media did not cause significant alterations in the studied characteristics. In terms of water relations, the best growing media to substitute the tree fern fiber were composed by blocks of pressed coconut bark or by mixtures of this material with charcoal or Eucalyptus grandis bark. Bark of E grandis alone or in mixture with charcoal did not give good results.

Presença de microrganismos na silagem de grãos úmidos de milho ensilado com diferentes proporções de sabugo

This experiment was carried out in the Microbiology Laboratory of UNESP-Jaboticabal, to evaluate the different species of microorganisms in high-moisture corn grain silage. The treatments were five percentages of corn cob in the silage (0, 5, 10, 15 and 20% DM) and four sampling periods after the opening of the silos (0, 2, 4 and 6 days), using a factorial arrangement in randomized block design with three replications. The growth of Lactobacillus was higher (P<0.01) in the silage prepared only with grains in relation to the other treatments. The presence of Clostridium differed (P<0.01) among the treatments, with values ranging from 1.30 and 3.32 log CFU/g of silage. It was concluded that the population of Lactobacillus was satisfactory to obtain a good fermentation of the silages, and the presence of corn cob facilitated the development of Clostridium and also of yeast and Enterobacteriaceae after the silos were opened.

Avaliação da silagem de grãos úmidos de milho

This work had the objective of evaluating the effects of different percentages of corn (Zea mays L.) cob on the quality of the silage of high-moisture corn grains. The following treatments were studied: percentages of corn cob in the silage (0, 5, 10, 15 and 20%); period of sampling after opening the silos (zero, two, four and six days). The factorial arrangement 5 × 4 was studied according to a completely randomized block design with three replications. The variables studied were not affected by the sampling period. The buffering capacity and the pH were not affected by the cob, while the percent soluble carbohydrates and amoniacal nitrogen increasing until 1.2 and 1.89 unit percent, respectively. Increasing the amount of corn cobs reduced the contents of DM (from 63.9% to 58.6%), CP (from 10.0% to 7.3%), EE (from 4.87% to 3.92%) and the values of DMIVD (from 90.5% to 79.1%) in the silages and increasing the contents of acid detergente fiber (ADF) (from 3.3% to 12.9%) and neutral detergente fiber (NDF) (from 15.16% to 26.1%). The values of brute energy (BE) were not affected (P>0.01) by the cob corn in the silage.

Aditivos na ensilagem do capim-elefante. II. Qualidade das silagens amostradas por dois métodos

Silages of Pennisetum purpureum Schum. cv. Guaçu prepared with 0, 8, 16 and 24% of ground ear com with husks, wheat bran and saccharin, dry weight of additive/wet weight of green chop basis. The experimental design was a randomized blocks one in split-plot; the plots were the additives and levels, and the sub-plots the sampling methods. The material was ensiled using plastic vessels in middle of which holed pvc pipes (3 inches diameter) were put. These pipes (one per vessel) had the same length as the height of the vessels, and were filled at the same time and compacted the same way as the vessels. The first method of sampling used the material ensiled inside the pvc pipe, which was lifted out from the vessel at the moment of the silo opening. The other sampling method, normaly used in digestibility trials, consisted of samples composed by daily sub-samples collected in the vessels. The pvc sampling method was more efficient because it sampled a profile of the whole silage. All of the silages showed high percentages of lactic acid and low percentages or even absence of butyric acid, though in all silages high ammoniacal-N percentages were detected.

Aditivos na ensilagem do capim-elefante. IV. Fermentação ruminal em ovinos

The ruminai fermentation patterns of sheep fed elephant grass (Pennisetum purpureum Schum.) silage enriched with ground ear corn with husks, wheat bran and saccharin in the levels 0, 8, 16 and 24% dry weight of additive/wet weight of green chop was evaluated. A split-plot randomized block design was used. The plots were the additives and their levels and the sub-plots the time of rumen fluid collection (0, 1, 3, 6, 9, 12 and 24 h after feeding). During the collection period, the sheep were fed 80% of the observed voluntary feed intake of the previous phase. For all additive types and levels used in preparing the silages, high levels of total volatile fat acids were observed, with predominance of the acetic acid. The silages having ground ear corn with husks as additive showed, in the ruminai fluid, ammonia production levels below the recommended for maximum microbial protein synthesis. However, silages with saccharin or wheat bran presented a good ammoniacal-N availability. In the ruminal fluid of the sheep fed ground ear corn with husks or wheat bran the molar proportion of butyric acid was increased and that of acetic acid and pH were decreased, as the levels of the additives in the silage increased.

A seqüência mafra superior \ Rio Do Sul inferior (Grupo itararé, permocarbonífero) em sondagens testemunhadas da região de mafra (SC), Margem Leste da Bacia do Paraná

The Permo-Carboniferous Harare Group crops out in the Matra area, represented by Campo do Tenente, Mafra and Rio do Sul formations; they correspond in subsurface to Lagoa Azul, Campo Mourão and Tacïba formations. A composite sampling of the Group was performed through drilling of six wells, which average 60 m in depth; three of them cored the depositional sequence here designated as Upper Mafra\Lower Rio do Sul. The Upper Mafra Formation were sampled by TC-4 and BR-5 wells, and it consists of three units: the lower two are sandy, glacial-deltaic and fluvial-deltaic in origin, corresponding to a lowstand tract. The last unit is composed of two dirtying-upward successions of sandstone, diamictite and rhythmite, interpreted as deglaciation/transgressive events, and well represented in BR-5 drilling. The Lower-Rio do Sul Formation (Lontras Shale) is formed by two marine units: the lower one is represented by shale and bioturbated siltstone which culminate the previous deglaciation, transgressive succession, while the upper one, sampled by SL-2 well, is formed by shale and thin, turbidite sandstone, attributed to a highstand tract.

Colorimetric determination of ambient ozone using indigo blue droplet

A simple and sensitive method based on a liquid droplet is described for the measurement of atmospheric ozone. A 30 μL drop of indigo blue solution is suspended in a flowing-air sampling stream. The ozone collected reacts with the indigo solution resulting in its decolorization. The colorimetric sensor is composed of two optical fibers and the source of monochromatic light was a red LED (625 nm). The calibration curve was constructed with ozone standard concentrations ranging from 37 - 123 ppbv. The detection limit achieved was 7.3 ppbv. The method considered here showed itself to be easy to apply with a fast response and a total analysis time of only 5 minutes.

Estatística de levantamentos epidemiológicos sobre cárie dentária, publicados no brasil e no exterior, de 1960 a 2001

The epidemiological surveys are important to the deployment, implementation and evaluation of projects and health actions in a community. The planning, goals, samples, team training/calibration, carrying out and publication of results are extremely important in the epidemiological surveys. Thus, the care with the sample and statistical analysis is fundamental for the results to be consistent and trustworthy in order to be able to be inferred for all the population. The aims of this study is to investigate the statistical methodology used in papers on dental caries epidemiological surveys published from 1960 to 2001. A bibliographical survey was carried out in BBO, MEDLINE and SCIELO databases. The papers found were analyzed with regards to the statistical methodology applied in the whole study, from the sampling to the tabling of data. Most studies (72.6%) only presented the number of elements that composed the sample, without explaining the planning involved in obtaining it.

Hidrogeoquímica do aqüífero guarani na área urbana de ribeirão preto (SP)

This work evaluated the hydrogeochemistry of the Guarani Aquifer in the urban area of Ribeirão Preto, São Paulo State. Thirty one sampling points were established and it was carried out field campaigns in 7 and 8 July 2005, analyzing the following parameters: conductivity, temperature, pH, turbidity, color, dry residue, dissolved oxygen, Ca2+, Mg2+, Na+, K+, Fe3+, Al3+, HCO3-, SO42-, PO43-, NO3-, Cl- and F-. The results indicated that the groundwaters in the urban area of Ribeirão Preto pH possess slightly acid, low conductivity, turbidity, color and dissolved oxygen inside the limits established for drinking waters. The ionic composition indicated that the groundwaters have low cations and anions concentration, being they classified as soft water and calcium-bicarbonated. Statistical tests suggested that conductivity and pH are controlled for the calcite dissolution (CaCO3) during the water/rock interaction process. Besides, it is possible to conclude that the high NO3- and PO43- concentrations found in the groundwater in the urban area of Ribeirão Preto are originated due to anthropogenic activities.

Análise espacial de nutrientes e produção de alface cultivada em ambiente protegido

The objective of this work was to study spatial variability of some chemical soil attributes and lettuce production (total shoot fresh matter mass - MF; commercial shoot fresh matter mass - MFC; commercial shoot dry matter mass - MCS; and head commercial diameter - DCC) offering subsidies to the protected environment mapping in nutrients management areas in lettuce culture aiming for a higher productivity with application of fertilizers. The experiment was conducted in a protective environment (greenhouse) with lettuce irrigated by drip irrigation and sampling grid with 152 points. The special dependence analysis, determined by the variogram, was obtained with the aid of the GS+ Program. Considering the need for crop nutrients through the map obtained for element P (phosphorus) it was possible to establish two distinct areas for the application of this element in plantation fertilization. Through the lettuce yield maps obtained with MFC and DCC attributes was difficult to establish distinct areas for its management with data observed in only one crop cycle. Krigagem has proved useful for mapping the attributes studied.

Assistência de ar e aplicação em volume baixo no controle da ferrugem asiática da soja (Phakopsora pachyrhizi Sydon & Sydon

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Adubação mineral, esterco de curral e lodo de esgoto no desenvolvimento inicial do cafeeiro

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)