Padronização da reação de imunofluorescência direta para detecção de oocistos de cryptosporidium parvum em amostras fecais de bezerros

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Preparação e caracterização de casearinas de Casearia sylvestris como padrão fitoquímico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação química e biológica do fungo endofítico Colletotrichum sp. isolado de Senna spectabilis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação química e biológica de fungos endofíticos associados à alga marinha Padina gymnospora

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Investigação fitoquímica e avaliação da atividade antioxidante das folhas e frutos de Eugenia jambolana Lam. (Myrtaceae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Identificação dos princípios ativos antiulcerogênicos das folhas de Casearia sylvestris: contribuição para o desenvolvimento de um fitoterápico

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo químico e biológico de plantas da família Eriocaulaceae

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo químico e atividade mutagênica e antirradicalar de caesalpinia ferrea

Pós-graduação em Química - IQ

Esteroide e Triterpenos de espécies de Qualea: Bioatividade sobre Mycobacterium tuberculosis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Esteroide e Triterpenos de espécies de Qualea: bioatividade sobre Mycobacterium tuberculosis

The chloroform extract of bark of the tropical tree Qualea parviflora (Vochysiaceae) was fractionated by column chromatography on silica gel, yielding triterpenes (lupeol, lupenone, betulin, epi-betulinic acid and friedelin) and a steroid (β-sitosterol). β-sitosterol, lupenone and lupeol were also identified in Q. grandiflora and Q. multiflora, while friedelin was detected only in Q. Multiflora, by means of gas chromatography-mass spectrometry. The anti-Mycobacterium tuberculosis activity of the chloroform extract and isolated compounds was assayed by MABA and MIC values ranged from 250.0 to 31.2 µg/mL. This study is the first to investigate the chemistry and antitubercular activity of apolar compounds from Qualea species.

Estudos sobre a aplicação do pentacloreto de nióbio na síntese de derivados de 4-aril-3,4-di- hidrocumarinas

Coumarin is a natural active compound that can be found in many plants. The coumarins have many properties such as bronchodilator, anti-inflammatory, antioxidant, anticoagulant, antibiotics, immunomodulatory, antimicrobial and antiviral, thus, they are widely used in medical applications. More recently the coumarin derivatives have attracted the interest of many research groups in the field of new materials, for example the possibility of their use as sensitizers in dye-sensitized solar cells (DSSC) and lasers. The MCRs are defined as a process in which three or more reactants are combined in the same reaction pot, resulting in products with good structural complexity a single step, in addition to economy of atoms and selectivity and is a very important feature in modern synthetic methodology. In this work we investigated the use of niobium pentachloride as catalyst of the multicomponent reactions between phenolic derivatives, various aromatic aldehydes and β-diester derivatives in the synthesis of 4-aryl-3,4-dihydrocoumarin derivatives. The reactions were carried out at room temperature, under inert atmosphere (N2), using dichloromethane anhydrous (CH2 Cl2) as solvent, with a reaction time of most 120 hours. The products were isolated by column chromatography on silica gel and submitted to spectrometric and spectroscopic analysis. The results show that NbCl5 is an excellent agent for promoting the synthesis of 4-aryl-3,4-dihydrocoumarin derivatives through multicomponent reactions, obtaining yields varying from 45 to 95%

Effects of the extract and glycoalkaloids of Solanum lycocarpum St. Hill on Giardia lamblia trophozoites

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Antiproliferative activity of cardenolide glycosides from Asclepias subulata

Asclepias subulata Decne. is a shrub occurring in Sonora-Arizona desert (Mexico-USA). The ethnic groups, Seris and Pimas, use this plant for the treatment of sore eyes, gastrointestinal disorders and cancer. To isolate the compounds responsible for antiproliferative activity of the methanol extract of A. subulata. A bioguided fractionation of methanol extract of A. subulata was performed using MTT assay to measure the antiproliferative activity of different compounds on three human cancer cell lines (A549, LS 180 and PC-3), one murine cancer cell line (RAW 264.7) and one human normal cell line (ARPE-19). The methanol extract was partitioned with hexane, ethyl acetate and ethanol. The active fractions, ethanol and residual, were fractioned by silica-column chromatography and active sub-fractions were separated using HPLC. The chemical structures of isolated compounds were elucidated with different chemical and spectroscopic methods. A new cardenolide glycoside, 12, 16-dihydroxycalotropin, and three known, calotropin, corotoxigenin 3-O-glucopyranoside and desglucouzarin, were isolated of active sub-fractions. All isolated compounds showed a strong antiproliferative activity in human cancer cells. Calotropin was the more active with IC50 values of 0.0013, 0.06 and 0.41 µM on A549, LS 180 and PC-3 cell lines, respectively; while 12, 16-dihydroxycalotropin reached values of 2.48, 5.62 and 11.70 µM, on the same cells; corotoxigenin 3-O-glucopyranoside had IC50 of 2.64, 3.15 and 6.62 µM and desglucouzarin showed values of 0.90, 6.57 and 6.62, µM. Doxorubicin, positive control, showed IC50 values of 1.78, 6.99 and 3.18 µM, respectively. The isolated compounds had a weak effect on murine cancer cells and human normal cells, exhibiting selectivity to human cancer cells. In this study, we found that 12, 16-dihydroxicalotropin, calotropin, corotoxigenin 3-O-glucopyranoside and desglucouzarin are responsible of antiproliferative properties of A. subulata, and that these compounds are highly selective to human cancer cells. Further studies are needed in order to establish the action mechanisms of the isolated compounds.

Avaliação das atividades cicatrizante e antiinflamatória de Casearia sylvestris Swartz em animais e identificação dos marcadores químicos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Diterpenos tetranorlabdanos e isocumarinas produzidos por Botryosphaeria parva, um fungo endofítico em Eugenia jambolana Lam. (Myrtaceae)

Pós-graduação em Química - IQ