Identification of sexually dimorphic gene expression in brain tissue of the fish Leporinus macrocephalus through mRNA differential display and real time PCR analyses

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

High expression of human monocyte iNOS mRNA induced by Paracoccidioides brasiliensis is not associated with increase in NO production

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

mRNA profile of Nellore calves after primary infection with Haemonchus placei

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The role of the seed coat in phytochrome-controlled seed germination in Lactuca sativa L. cv. Grand Rapids

The effect of scarification with sodium hypochloride on light sensitivity in seeds of Lactuca sativa L. cv. Grand Rapids is presented in the paper. Light-requiring lettuce seeds germinated in both dark and continuous light after scarification and 36 degrees C pre-incubation restored light sensitivity at 25 degrees C. Curves of dose-response indicated that chemical scarification induced a change in the control of seed germination from Low Fluence Response to the Very Low Fluence Response. Pre-incubation at 36 degrees C return the control to the Low Fluence Response of phytochrome action.

The germination of seeds of Epiphyllum phyllanthus (L.) Haw. (Cactaceae) is controlled by phytochrome and by nonphytochrome related process

As sementes de Epiphyllum phyllanthus apresentam alta sensibilidade à luz e a sua germinação pode ser promovida pela luz verde de segurança por meio da resposta de fluência muito baixa mediada pelo fitocromo A. Parte da população de sementes tem fitocromo B na forma ativa (Fve) suficiente para promover a germinação no escuro. Sementes de Epiphyllum phyllanthus germinam em uma ampla faixa de temperatura de 10 a 40°C, atingindo germinação completa na faixa de 15 a 30°C. Acima de 35°C a velocidade de germinação aumenta indicando o controle por um processo não relacionado com o fitocromo. A análise da cinética da germinação de sementes indicou que o controle pelo fitocromo A é menos dependente da temperatura do que o processo controlado pelo fitocromo B.

Phytochrome controls achene germination in Bidens pilosa L. (Asteraceae) by very low fluence response

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Expression of annexin A1 mRNA in peripheral blood from oral squamous cell carcinoma patients

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Phytochrome and temperature control of seed germination in Muntingia calabura L. (Elaeocarpaceae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

New proposal of classification of seeds based on forms of phytochrome instead of photoblastism

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

PHYTOCHROME CONTROLLED SEED-GERMINATION IN LIGHT INSENSITIVE SEEDS OF RAPHANUS-SATIVUS L CV REDONDO GIGANTE

Seed germination of Raphanus sativus is inhibited by light only under low water potential of the medium. Curves of fluence response to white, red and far-red lights demonstrated that the inhibition of seed germination is dependent on light irradiance being far-red light the more effective than the formers and the germination being inhibited by intermitent light indicates phytochrome involvement through the high irradiance reactions in the control of seed germination in radish seeds under water stress conditions.

Effects of dietary macronutrient content on energy metabolism and uncoupling protein mRNA expression in broiler chickens

The objective of the present study was to investigate the effects of dietary macronutrient ratio on energy metabolism and on skeletal muscle mRNA expression of avian uncoupling protein (UCP), thought to be implicated in thermogenesis in birds. Broiler chickens from 2 to 6 weeks of age received one of three isoenergetic diets containing different macronutrient ratios (low-lipid (LL) 30 v. 77 g lipid/kg-, low-protein (LP) 125 v. 197 g crude protein (N X 6.25)/kg; low-carbohydrate (LC) 440 v. 520 g carbohydrate/kg). LP chickens were characterised by significantly lower body weights and food intakes compared with LL and LC chickens (-47 and -38% respectively) but similar heat production/kg metabolic body weight, as measured by indirect calorimetry, in the three groups. However, heat production/g food ingested was higher in animals receiving the LP diet (+41%, P<0.05). These chickens also deposited 57% less energy as protein (P<0.05) and 33% more as fat. No significant differences in energy and N balances were detected between LL and LC chickens. The diets with the higher fat contents (i.e. The LP and LC diets) induced slightly but significantly higher relative expressions of avian UCP mRNA in gastrocnemius muscle, measured by reverse transcription-polymerase chain reaction, than the LL diet (88 and 90 v. 78% glyceraldehyde-3-phosphate dehydrogenase respectively, P<0.05). Our present results are consistent with the recent view that UCP homologues could be involved in the regulation of lipid utilisation as fuel substrate and provide evidence that the macronutrient content of the diet regulates energy metabolism and especially protein and fat deposition.

Expression of LH receptor mRNA splice variants in bovine granulosa cells: Changes with follicle size and regulation by FSH in vitro

In cattle, most evidence suggests that granulosa cells express LH receptors (LHR) after (or as) the follicle becomes dominant, however there is some suggestion that granulosa cells from smaller pre-dominant follicles may express several LHR mRNA splice variants. The objective of this study was to measure LHR expression in bovine follicles of defined size and steroiclogenic ability, and in granulosa cells from small follicles (< 6 mm diameter) undergoing differentiation in vitro. Serniquantitative RT-PCR demonstrated that LHR mRNA was undetectable in granulosa cells of follicles < 7 mm diameter (nondominant follicles), and increased with follicle diameter in follicles > 7 mm diameter. Splice variants with deletions of exon 10 and part of exon 11 were detected as previously described, and we detected a novel splice variant with a deletion of exon 3. Cultured granulosa cells contained LHR mRNA, but with significantly greater amounts of variants with deletions of exon 10 and/or exon 11 compared with cells from dominant follicles. FSH increased the abundance of some but not all LHR mRNA splice variants in cultured granulosa cells. The addition of LH to cultured cells did not increase progesterone secretion, despite the presence of LHR mRNA. Collectively, these data suggest that granulosa cells do not acquire functional LHR until follicle dominance occurs.

Pulmonary Disease in Hamsters Infected with Leptospira interrogans: Histopathologic Findings and Cytokine mRNA Expressions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)