Germinação das unidades de dispersão de gervão-roxo

Foram conduzidos dois experimentos com o objetivo de estudar a germinação das unidades de dispersão de gervão-roxo [Stachytarpheta cayennesis (L.C. Rich) Vahl], removidas de espigas que haviam sido armazenadas após terem sido colhidas em dois estádios de maturação. As unidades de dispersão foram removidas de espigas que se encontravam armazenadas por seis ou 18 meses e que haviam sido colhidas com a coloração verde ou palha. No primeiro experimento, as unidades de dispersão, incubadas no interior de caixas plásticas tipo gerbox, foram distribuídas em folhas de papel germitest umedecidas com água destilada, sob temperatura de 20ºC(noite) e 30ºC (dia), na presença ou ausência de fotoperíodo de 12 horas, por 28 dias. No segundo experimento, as unidades de dispersão, incubadas no interior de caixas plásticas, foram distribuídas em folhas de papel germitest umedecidas com água destilada ou com solução de KNO3, sob temperatura de 20ºC(noite) e 30ºC (dia), na presença ou ausência de fotoperíodo de 12 horas, por 28 dias. A maior germinação foi obtida a partir de unidades de dispersão provenientes de espigas colhidas com a coloração palha e que se encontravam armazenadas por seis meses. A presença da luz, e o emprego de nitrato na ausência de luz, favoreceram a germinação das unidades de dispersão retiradas da parte apical e basal de espigas colhidas com a coloração verde ou palha e, armazenadas por seis ou 18 meses.

Aprendizado não-supervisionado em redes neurais pulsadas de base radial: um estudo da capacidade de agrupamento para a classificação de pixels

Redes neurais pulsadas - redes que utilizam uma codificação temporal da informação - têm despontado como uma promissora abordagem dentro do paradigma conexionista, emergente da ciência cognitiva. Um desses novos modelos é a rede neural pulsada com função de base radial, que é capaz de armazenar informação nos tempos de atraso axonais dos neurônios. Um algoritmo de aprendizado foi aplicado com sucesso nesta rede pulsada, que se mostrou capaz de mapear uma seqüência de pulsos de entrada em uma seqüência de pulsos de saída. Mais recentemente, um método baseado no uso de campos receptivos gaussianos foi proposto para codificar dados constantes em uma seqüência de pulsos temporais. Este método tornou possível a essa rede lidar com dados computacionais. O processo de aprendizado desta nova rede não se encontra plenamente compreendido e investigações mais profundas são necessárias para situar este modelo dentro do contexto do aprendizado de máquinas e também para estabelecer as habilidades e limitações desta rede. Este trabalho apresenta uma investigação desse novo classificador e um estudo de sua capacidade de agrupar dados em três dimensões, particularmente procurando estabelecer seus domínios de aplicação e horizontes no campo da visão computacional.

Efeitos de potenciais de água no solo, em diferentes estádios fenológicos da cultura da cevada (Hordeum vulgare L.)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Evaluation and application of bismuth as an internal standard for the determination of lead in wines by simultaneous electrothermal atomic absorption spectrometry

A method for the direct determination of Pb in wines by simultaneous multi-element atomic absorption spectrometry (SIMAAS) using a transversely heated graphite atomizer, Zeeman-effect background corrector and internal standardization is proposed. Bismuth was used as an internal standard and Pd(NO3)(2) plus Mg(NO3)(2) as chemical modifier,to stabilize both the analyte and the internal standard. The implementation of two pyrolysis steps avoided any build-up of carbonaceous residues on the graphite platform. All diluted samples (1 + 1 v/v) in 0.2% v/v HNO3 and reference solutions (5.0-50 mug l(-1) Ph in 0.2% v/v HNO3) were spiked with 25 mug l(-1) Bi. For a 20 mul aliquot dispensed into the graphite tube, a good correlation (r = 0.9997) was obtained between the ratio of the analyte signal to the internal standard signal and the analyte concentration of the reference solutions. The electrothermal behaviour of Pb and Bi in red, white and rose wines were compared. In addition, absorbance variations due to changes in experimental conditions, such as atomizer temperature, integration time, injected sample volume, radiation beam intensity, graphite tube surface, dilution and sample composition, were minimized by using Bi as internal standard. Relative standard deviations of measurements based on integrated absorbance varied from 0.1 to 3.4% and from 0.5 to 7.3% (n = 12) with and without internal standard correction, respectively. Good recoveries (91-104%) for Pb spikes were obtained. The characteristic mass was 45 pg Pb and the limit of detection based on integrated absorbance was 0.9 mug l(-1) Pb. Internal standardization increased the lifetime of the tube by 25%. Direct determinations of Pb in wines with and without internal standardization approaches were in agreement at the 95% confidence level. The repeatability and the tube lifetime were improved when using Bi as internal standard. The improvement in accuracy using an internal standard was only observed when the analytical results were affected by errors.

Electrothermal AAS determination of Ni in soft drinks using Co as the internal standard

A method for the direct determination of Ni in soft drinks by graphite furnace atomic absorption spectrometry using a transversely heated graphite atomizer (THGA), Zeeman-effect background corrector, and Co as the internal standard (IS) is proposed. Magnesium nitrate was used to stabilize both Ni and Co. All diluted samples (1+1) in 0.2% (v/v) HNO3 and reference solutions [5.0-50 mu g L-1 Ni in 0.2% (v/v) HNO3] were spiked with 50 mu g L-1 Co. For a 20-mu L sample dispensed into the atomizer, correlations between the ratio of absorbance of Ni to absorbance of Co and the analyte concentration were close to 0.9996. The relative standard deviation of the measurements varied from 0.5 to 3.4% and 1.0 to 7.0% (n=12) with and without IS, respectively. Recoveries within 98-104% for Ni spikes were obtained using IS. The characteristic mass was calculated as 43 pg Ni and the limit of detection was 1.4 mu g L-1. The accuracy of the method was checked for the direct determination of Ni in soft drinks and the results obtained with IS were better than those without IS.

Differential effects of novel wasp toxin on rat hippocampal interneurons

We studied the effects of a wasp toxin beta-pompilidotoxin (beta-PMTX) on rat hippocampal CA1 interneurons by the current-clamp technique. The firing patterns of pyramidal neurons and pyramidale interneurons were not affected by beta-PMTX, but in oriens and radiatum interneurons, beta-PMTX converted the action potentials to prolonged depolarizing potentials by slowing the inactivation of Na+ channels. In lacunosum moleculare interneurons, beta-PMTX induced initial bursting spikes followed by block of succeeding spikes. Comparison of beta-PMTX with a sea anemone toxin, ATX 11, revealed that ATX 11 altered the firing properties of pyramidal neurons and pyramidale interneurons that were unchanged by beta-PMTX. Our results suggest that beta-PMTX modulates Na+ currents in CAl interneurons differently in various CAl neurons and the toxin is useful to classify Na+ channel subtypes. (C) 2002 Elsevier B.V. Ireland Ltd. All rights reserved.

Minimization of lead and copper interferences on spectrophotometric determination of cadmium using electrolytic deposition and ion-exchange in multi-commutation flow system

A new strategy for minimization of Cu2+ and Pb2+ interferences on the spectrophotometric determination of Cd2+ by the Malachite green (MG)-iodide reaction using electrolytic deposition of interfering species and solid phase extraction of Cd2+ in flow system is proposed. The electrolytic cell comprises two coiled Pt electrodes concentrically assembled. When the sample solution is electrolyzed in a mixed solution containing 5% (v/v) HNO3, 0.1% (v/v) H2SO4 and 0.5 M NaCl, Cu2+ is deposited as Cu on the cathode, Pb2+ is deposited as PbO2 on the anode while Cd2+ is kept in solution. After electrolysis, the remaining solution passes through an AG1-X8 resin (chloride form) packed minicolumn in which Cd2+ is extracted as CdCl4/2-. Electrolyte compositions, flow rates, timing, applied current, and electrolysis time was investigated. With 60 s electrolysis time, 0.25 A applied current, Pb2+ and Cu2+ levels up to 50 and 250 mg 1-1, respectively, can be tolerated without interference. For 90 s resin loading time, a linear relationship between absorbance and analyte concentration in the 5.00-50.0 μg Cd 1-1 range (r2 = 0.9996) is obtained. A throughput of 20 samples per h is achieved, corresponding to about 0.7 mg MG and 500 mg KI and 5 ml sample consumed per determination. The detection limit is 0.23 μg Cd 1-1. The accuracy was checked for cadmium determination in standard reference materials, vegetables and tap water. Results were in agreement with certified values of standard reference materials and with those obtained by graphite furnace atomic absorption spectrometry at 95% confidence level. The R.S.D. for plant digests and water containing 13.0 μg Cd 1-1 was 3.85% (n = 12). The recoveries of analyte spikes added to the water and vegetable samples ranged from 94 to 104%. (C) 2000 Elsevier Science B.V.

Compactação do solo e intervalo hídrico ótimo no crescimento da parte aérea e produtividade da cultura do milho

The aim of this work was to evaluate the effect of soil compaction caused by tractor wheel traffic on the limiting water range (LLWR), shoot growth and levels of compaction and four replications in a completely randomized experimental design. Soil samples with preserved structure were collected in the layers: 0.02-0.05; 0.08-0.11; 0.15-0.18 and 0.22-0.25m to determine macroporosity, microporosity, total porosity, bulk density, resistance to penetration and LLWR. The evaluated corn parameters were: plant and first spike height, steam diameter, number of spikes per plant, plant dry matter, dry matter of 1000 seeds and seed productivity. The soil compaction restricted all corn parameters except the number of spikes per plant and dry matter of 1000 seeds. The LLWR was reduced by the soil resistance to penetration, even in the tilled soil with bulk density of 1.12 Mg m-3. Only the treatment with 11 Mg tractor, repeated four times on the area, demonstrated bulk density above critical bulk density in the LLWR that was 1.37 Mg m-3, where the seed productivity was significantly smaller.

Infantile epileptic encephalopathy with hypsarrhythmia (infantile spasms/west syndrome) and immunity

West syndrome is a severe epilepsy, occurring in infancy, that comprises epileptic seizures known as spasms, in clusters, and a unique EEG pattern, hypsarrhythmia, with psychomotor regression. Maturation of the brain is a crucial component. The onset is within the first year of life, before 12 months of age. Patients are classified as cryptogenic (10 to 20%), when there are no known or diagnosed previous cerebral insults, and symptomatic (80 to 90%), when associated with pre-existing cerebral damages. The time interval from a brain insult to infantile spasms onset ranged from 6 weeks to 11 months. West syndrome has a time-limited natural evolutive course, usually disappearing by 3 or 4 years of age. In 62% of patients, there are transitions to another age-related epileptic encephalopathies, the Lennox-Gastaut Syndrome and severe epilepsy with multiple independent foci. Spontaneous remission and remission after viral infections may occur. Therapy with ACTH and corticosteroids are the most effective. Reports about intravenous immunoglobulins action deserve attention. There is also immune dysfunction, characterized mainly by anergy, impaired cell-mediated immunity, presence of immature thymocytes in peripheral blood, functional impairment of T lymphocytes induced by plasma inhibitory factors, and altered levels of immunoglobulins. Changes in B lymphocytes frequencies and increased levels of activated B cells have been reported. Sensitized lymphocytes to brain extract were also described. Infectious diseases are frequent and may, sometimes, cause fatal outcomes. Increase of pro-inflamatory cytokines in serum and cerebrospinal fluid of epileptic patients were reported. Association with specific HLA antigens was described by several authors (HLA-DR7, HLA-A7, HLA-DRw52, and HLA-DR5). Auto-antibodies to brain antigens, of several natures (N-methyl-d-aspartate glutamate receptor, gangliosides, brain tissue extract, synaptic membrane, and others), were described in epileptic patients and in epileptic syndromes. Experimental epilepsy studies with anti-brain antibodies demonstrated that epileptiform discharges can be obtained, producing hyperexcitability leading to epilepsy. We speculate that in genetically prone individuals, previous cerebral lesions may sensitize immune system and trigger an autoimmune disease. Antibody to brain antigens may be responsible for impairment of T cell function, due to plasma inhibitory effect and also cause epilepsy in immature brains. © 2008 Bentham Science Publishers Ltd.

Características morfoagronômicas do milho submetido a diferentes níveis de desfolha manual

The present study was conducted to evaluate the damage caused by defoliation in maize. The experiment was carried out in 2005/2006 at Roma Farm, Ituverava-SP, using the triple hybrid CODETEC 304®. The experiment consisted of split plots (split-plot), which were distributed at random in three blocks, with the main treatment manual defoliation (30%, 40%, 60% and 80% defoliation) in the plots and as the secondary treatment the phenological phases (D1, D2 and D3) with three replicates for each treatment and a control plot (0% defoliation) for each block. It was evaluated the production of plots (g/m2), the average size of the spikes (cm) and the average weight of thousand grains (g). According to the results, it was concluded that the source-sink relations were negatively affected, and the treatment with 80% defoliation was the one that most affected all variables, thus, it is a level of defoliation that is responsible for the largest losses, with no means of compensation for the maize crop.

Molecular Dynamics simulations of track formation at different ensembles

A series of Molecular Dynamics simulations of thermal spikes has been run in zircon. For two different ensembles: microcanonical one and a combination of microcanonical one acting on the simulation core with Langevin one on the side walls of simulation. Depending on the used ensemble, different track-formation threshold energies were found. When the combined ensemble is carried out, the total energy of the simulations varies with the temperature which can influence how annealing fission-track models should deal with the lattice recovery. A fission-track annealing model is tested with the simulation results. © 2012 Elsevier Ltd. All rights reserved.

Comportamento produtivo do trigo em função da densidade de semeadura e da aplicação de reguladores vegetais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Períodos críticos de défices hídricos em cultivares de cevada (Hordeum vulgare L.)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Produtividade e valor nutritivo das plantas de milho de textura dentada ou dura em três maturidades para silagem

Pós-graduação em Zootecnia - FMVZ

Qualidade fisiológica da semente e adubação com zinco na cultura do milho-doce

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)