75 resultados para genetically modified mice


Relevância:

80.00% 80.00%

Publicador:

Resumo:

The study aimed to evaluate the occurrence of pest and management of cotton genetically modified with the introduction of the bacterium Bacillus thunringiensis compared to conventional cotton in the 'Cerrado' region, installing the field experiment in 2007/2008 in Chapadão do Sul-MS. The experimental design was randomized blocks in a 2 × 3 factorial, with two managements of insecticides (with and without insecticides to control lepidopteran) and 3 modes of use of cultivars: 100% transgenic (NUOPAL), 100% non transgenic (Deltaopal) and interior area with transgenic (80% NUOPAL) with non-transgenic border (20% Deltaopal) with five replications. Evaluations were performed weekly of pest infestation, observing in 15 plants per plot the number of Alabama argillacea, Heliothis virescens and Spodoptera frugiperda. Based on these results it was concluded that: the occurrence of A. argillacea and H. virescens was lower in treated transgenic cultivar. There were no differences between transgenic and conventional farming on the occurrence of S. frugiperda.

Relevância:

80.00% 80.00%

Publicador:

Resumo:

The polyphagous pests belonging to the genus Spodoptera are considered to be among the most important causes of damage and are widely distributed throughout the Americas'. Due to the extensive use of genetically modified plants containing Bacillus thuringiensis genes that code for insecticidal proteins, resistant insects may arise. To prevent the development of resistance, pyramided plants, which express multiple insecticidal proteins that act through distinct mode of actions, can be used. This study analyzed the mechanisms of action for the proteins Cry1Ia10 and Vip3Aa on neonatal Spodoptera frugiperda, Spodoptera albula, Spodoptera eridania and Spodoptera cosmioides larvae. The interactions of these toxins with receptors on the intestinal epithelial membrane were also analyzed by binding biotinylated toxins to brush border membrane vesicles (BBMVs) from the intestines of these insects. A putative receptor of approximately 65. kDa was found by ligand blotting in all of these species. In vitro competition assays using biotinylated proteins have indicated that Vip3Aa and Cry1Ia10 do not compete for the same receptor for S. frugiperda, S. albula and S. cosmioides and that Vip3Aa was more efficient than Cry1Ia10 when tested individually, by bioassays. A synergistic effect of the toxins in S. frugiperda, S. albula and S. cosmioides was observed when they were combined. However, in S. eridania, Cry1Ia10 and Vip3Aa might compete for the same receptor and through bioassays Cry1Ia10 was more efficient than Vip3Aa and showed an antagonistic effect when the proteins were combined. These results suggest that using these genes to develop pyramided plants may not prove effective in preventing the development of resistance in S. eridiana. © 2012 Elsevier Inc.

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Pós-graduação em Ciências Biológicas (Microbiologia Aplicada) - IBRC

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)