23 resultados para citometría de flujo


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Pós-graduação em História - FCHS

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The private for-profit institution necessarily works with a focus on continuous improvement of the results of its operations, thus ensuring the entry of assets. The archive is often consulted in order to obtain answers to questions of legal, administrative, political and diplomatic. This study aimed to delineate the organizational structure of a renowned brazilian company in the segment of photography in order to meet the tasks, activities and routines specific to the industry named “archive albums”, contextualizing it and providing elements for an effective organization of the ser-vices provided by industry. The study opted for a descriptive-exploratory method through observation of daily routines and interviews with the application of semi-structured questionnaire to industry officials and heads of departments. The results of data collection were synthesized by flow chart and a functional organizational structure that allows us to understand the activities developed by the sector.

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The main objective of this work was to mount and test an experimental model to measure the hydraulic conductance of ex vivo dentin. Seventeen healthy third molars, with indication of extraction of healthy donors aged between 15 and 30 years were obtained by informed consent. After cleaning them, disinfecting them, including them in resin epoxy and cutting them, there were 17 samples of dentin, corresponding to a disk of resin with a coronal section of tooth showing the dentin exposed on both sides of it. Three machines to measure the hydraulic conductance of the dentin were assembled according to the description of the model of Pashley. Samples were installed in a Chamber of diffusion, connected by using silicone tubes to a graduated transfer pipette and a 20cm water column. Through the displacement of a bubble of water in the inside of the pipette, the hydraulic conductance of each sample was measured 3 times on the 14th, 21st, 28th and 35th day post extraction. The data were tabulated and analyzed statistically. There is no SS difference in the rate of flow of a measured sample in the three machines (p=0.5937). There is no SS difference in measurements of the hydraulic conductance of 13 samples of human dentin measured in days 14, 21, 28 and 35 postextraction (p=0.0704). It is possible to mount an experimental model to study the hydraulic conductance of dentin ex vivo, based on the model of Pashley. The model seems to be reliable, but more research is needed in order to validate its reliability.

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Abstract This paper discusses the quality of basic education legitimized by the external evaluation and publicized by the Basic Education Development Index (IDEB) - an index that combines the performance of students in the assessment and the school flow, culminating with the establishment of an objective parameter (grade) that aims to reflect the Brazilian educational quality. The arguments are based on the premise that these two elements are incipient to determine the quality of education in the country without taking into account other factors that affect quality, namely: socio-economic and cultural level of the students, teacher training, enhancement of teaching, tangible and intangible working conditions, school management, school infrastructure, supplies, etc. The reflection leading to the debate on the lack of evidence to establish the quality of education travels the ways of the external evaluation in Brazil, the technical rationality of the school management referenced primarily by the IDEB and its governance to achieve the goals determined by this index. The purpose of this essay is to contribute to the current debate on the IDEB as a proposer and mobilizer of policies for the Brazilian public schools.

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The history of communication as a science was marked by different moments. The first one was built by technological experimentation and later, by language transformation. But the consolidation of communication as applied social science has come from theoretical research, when Lasswell and Lazarsfeld respectively proposed the hypodermic and the two-step flow theories. However, applied research – which involves experimentation and its observation in a practical application process – is once again being targeted by the academy. This text develops an essay on the importance of applied research to the development of contemporary communication, presenting examples and methods related to such reality.

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La popularización de los medios digitales y los recursos de comunicación difunde las narrativas no lineales y agrava la crisis de la enseñanza en el aula, con la expansión de diferentes modelos presenciales virtuales de la educación (PVE). Es evidente la demasificación de los nuevos medios, en un contexto que agudiza la percepción individual y social de los individuos, que son atraídos por el enorme flujo global de mensajes informativos (BRIGGS; BURKE, 2006). La transición de los métodos de enseñanza en el aula para modelar la educación virtual e interactiva ganó un nuevo formato en la educación pública en São Paulo en 2012, con el lanzamiento de cursos electivos de enseñanza de Inglés y Español para estudiantes de la secundaria, con la mediación de el PC y con el contenido producido por la Escuela Virtual de Programas Educativos del Estado de São Paulo - Evesp. Aún continua presencia en los salones de las grandes clases del sistema escolar, la falta de materiales de enseñanza y aprendizaje adecuados en las clases impartidas por los profesores cuyos repertorios están alejados de la vida cotidiana de los estudiantes. En este contexto, se comprueban las actuaciones de aprendizaje cualitativos más bien negativos de las cuatro habilidades comunicativas: escuchar, hablar, leer y escribir (CAMERON, 2001). La investigación que llevamos a cabo es analizar las experiencias de enseñanza de Inglés y Español en la plataforma computarizada Evesp y de la clase de Inglés y Español de los Centros para el Curso de idiomas (CELS), para la escuela secundaria, y el piloto Early Bird, de enseñanza y el aprendizaje de Inglés de la escuela primaria, todos bajo la Junta de Educación de Bauru (SP).

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Cryopreservation of sperm is important to preserve the germplasm from animals of genetic value, which can die unexpectedly. This study compares conventional and automated methods of cryopreservation of spermatozoa obtained from the epididymis of bulls post-mortem. Twenty-two epididymides were obtained from a commercial slaughterhouse. Spermatozoa were collected from the tail of the epididymis using the retrograde flow technique. Thus, the samples, which were diluted in 10 ml of extender without glycerol (Botubov® I, Botupharma, Botucatu, SP, Brazil), were evaluated on motility, sperm vigor, structural and functional (swelling hypoosmotic test) membrane integrity, mitochondrial activity, sperm viability and ADN fragmentation. The samples were divided into two aliquots and diluted in extender with glycerol (Botubov® II, Botupharma, Botucatu, SP, Brazil) at a concentration of 50x106 motile sperm/0.5 French straws. One sample was frozen by the conventional method (4 hours at 5°C, in a refrigerator and 20 min in nitrogen vapor) and the other by the automated method (Cryogen® Dualflex, Neovet, Uberaba, MG, Brazil). The parameters were higher in all the tests of fresh sperm samples, with the exception of the swelling hypoosmotic test, which showed no significant difference when the results were compared with sperm frozen by the conventional method. The average motility of fresh spermatozoa was 74%, and conventional and automated averages were 29 and 25%, respectively. Therefore, although cryopreservation techniques reduce sperm quality parameters, the viability of the sperm is maintained, and these methods can be used to preserve sperm.