Leucine-rich diet alters the eukaryotic translation initiation factors expression in skeletal muscle of tumour-bearing rats

Background: Cancer-cachexia induces a variety of metabolic disorders on protein turnorver, decreasing protein synthesis and increasing protein degradation. Controversly, insulin, other hormones, and branched-chain amino acids, especially leucine, stimulate protein synthesis and modulate the activity of translation initiation factors involved in protein synthesis. Since the tumour effects are more pronounced when associated with pregnancy, ehancing muscle-wasting proteolysis, in this study, the influence of a leucine-rich diet on the protein synthesis caused by cancer were investigated. Methods: Pregnant rats with or without Walker 256 tumour were distributed into six groups. During 20 days of experiment, three groups were fed with a control diet: C - pregnant control, W - tumour-bearing, and P - pair-fed, which received the same amount of food as ingested by the W group; three other groups of pregnant rats were fed a leucine-rich diet: L - pregnant leucine, WL - tumour-bearing, and PL - pair-fed, which received the same amount of food as ingested by the WL group. Results: The gastrocnemius muscle of WL rats showed increased incorporation of leucine in protein compared to W rats; the leucine-rich diet also prevented the decrease in plasma insulin normally seen in W. The expression of translation initiation factors increased when tumour-bearing rats fed leucine-rich diet, with increase of ∼35% for eIF2α and eIF5, ∼17% for eIF4E and 20% for eIF4G; the expression of protein kinase S6K1 and protein kinase C was also highly enhanced. Conclusion: The results suggest that a leucine-rich diet increased the protein synthesis in skeletal muscle in tumour-bearing rats possibly through the activation of eIF factors and/or the S6kinase pathway. © 2007 Ventrucci et al; licensee BioMed Central Ltd.

Uma discussão sobre documento audiovisual enquanto patrimônio arquivístico cultural no Brasil

The origin of the concept of cultural heritage and its definition and applicability to the Brazilian scenery is analyzed, with a focus on audiovisual archives.

The eukaryotic translation initiation factor 3 subunit L protein interacts with Flavivirus NS5 and may modulate yellow fever virus replication

Background: Yellow fever virus (YFV) belongs to the Flavivirus genus and causes an important disease. An alarming resurgence of viral circulation and the expansion of YFV-endemic zones have been detected in Africa and South America in recent years. NS5 is a viral protein that contains methyltransferase and RNA-dependent RNA polymerase (RdRp) domains, which are essential for viral replication, and the interactions between NS5 and cellular proteins have been studied to better understand viral replication. The aim of this study was to characterize the interaction of the NS5 protein with eukaryotic translation initiation factor 3 subunit L (eIF3L) and to evaluate the role of eIF3L in yellow fever replication. Methods. To identify interactions of YFV NS5 with cellular proteins, we performed a two-hybrid screen using the YFV NS5 RdRp domain as bait with a human cDNA library, and RNApol deletion mutants were generated and analyzed using the two-hybrid system for mapping the interactions. The RNApol region involved was segmented into three fragments and analyzed using an eIF3L-expressing yeast strain. To map the NS5 residues that are critical for the interactions, we performed site-direct mutagenesis in segment 3 of the interaction domain (ID) and confirmed the interaction using in vitro assays and in vivo coimmunoprecipitation. The significance of eIF3L for YFV replication was investigated using eIF3L overexpression and RNA interference. Results: In this work, we describe and characterize the interaction of NS5 with the translation factor eIF3L. The interaction between NS5 and eIF3L was confirmed using in vitro binding and in vivo coimmunoprecipitation assays. This interaction occurs at a region (the interaction domain of the RNApol domain) that is conserved in several flaviviruses and that is, therefore, likely to be relevant to the genus. eIF3L overexpression and plaque reduction assays showed a slight effect on YFV replication, indicating that the interaction of eIF3L with YFV NS5 may play a role in YFV replication. Conclusions: Although the precise function of eIF3L on interactions with viral proteins is not entirely understood, these results indicate an interaction of eIF3L with YF NS5 and that eIF3L overexpression facilitates translation, which has potential implications for virus replication. © 2013 Morais et al.; licensee BioMed Central Ltd.

Supernatural na web: produção e reprodução audiovisual em suporte convergente

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

José Louzeiro, do romance-reportagem ao cinema: estudo da adaptação literária para o audiovisual a partir de Lúcio Flávio e Infância dos Mortos

Pós-graduação em Comunicação - FAAC

Televisão digital e Educação a distância: modelo de ambiente virtual de aprendizagem para mídia audiovisual

Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

TV digital sob a perspectiva publicitária: uma análise do advertainment na narrativa audiovisual

Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

As contribuições do audiovisual para a formação de jovens produtores: a construção de conteúdos por meio de dispositivos móveis

Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

Canais públicos de televisão digital e as possibilidades de arranjos produtivos locais do audiovisual

Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

Experiência audiovisual e infância: em busca do que escapa ao primeiro olhar

Pós-graduação em Educação - IBRC

Sexualidade(S) em cena: as contribuições do discurso audiovisual para a problematização das diferenças no espaço escolar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Abordagens do documento audiovisual no campo teórico da arquivologia

Pós-graduação em Ciência da Informação - FFC

Mídias e Linguagem Audiovisual: investigando possibilidades na prática pedagógica do professor de Educação Física

Pós-graduação em Educação - FCT

Memória audiovisual da reforma psquiátrica no Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ginástica na escola e a utilização da tecnologia audiovisual (vídeo)

Pós-graduação em Desenvolvimento Humano e Tecnologias - IBRC