Lentinula edodes (Shiitake) modulates chemically induced mutagenesis by enhancing pitting

This study was undertaken to understand how Lentinula edodes modulates in vivo mutagenesis induced by alkylating agents in bone marrow and peripheral blood as described in our previous article. Male Swiss mice were pretreated for 15 consecutive days with aqueous extracts prepared from L. edodes, after which, the number of circulating blood cells, normal erythroid bone marrow cell cycling, and phagocytosis of micronucleated reticulocyte (MNRET) and activation of spleen macrophages were assessed. The results indicate that the antimutagenicity seen in bone marrow and peripheral blood is exerted by distinct compounds with different actions. The antimutagenic effect in bone marrow is exerted by compounds subject to degradation at deep-freeze storage temperature of -20 C. On the other hand, compounds responsible for antimutagenicity in peripheral blood are not subject to degradation at -20 C. The results also indicate that the antimutagenic action in peripheral blood leading to the reduction of circulating MNRET occurs in the spleen primarily through a phagocytic activity due to higher macrophage numbers and probably not due to the enhanced activation state of individual cells. © Mary Ann Liebert, Inc.

Atividade antioxidante dos extratos de shiitake (Lentinus edodes) e de cogumelo do sol (Agaricus blazei) aplicados em óelo de soja sob aquecimento

Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

Controle de fungos contaminantes no cultivo do cogumelo comestível shiitake (Lentinula edodes) em toros de eucalipto (Eucalyptus urophylla)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Crescimento micelial, produção e características bromatológicas do shiitake em função de linhagens e de propriedades físicas e químicas de espécies e clones de eucalipto

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito de linhagens e substratos no crescimento miceliano e na produtividade em cultivo axênico de shiitake (Lentinula edodes (Berk.) Pegler)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Uso de resíduos de madeiras e frutos da amazônia para o cultivo in vitro do cogumelo comestível shiitake

Local abundant residues with low or without commercial value with potential to make the production of edible mushrooms economically viable in any region of Brazil must be employed for cultivation. Thus, the radial mycelial growth of LED 20 strain of Lentinula edodes (Berk.) Pegler was verified in culture media prepared with extract of regional residues by using substrates based on Protium puncticulatum, Cariniana micrantha and Caryocar glabum sawdust, supplemented with 20% of grinded residues from the barks of the fruits of Astrocaryum aculeatum and Theobroma grandiflorum. Residues from eucalyptus (Eucalyptus spp) sawdust and wheat bran (Triticum aestivum L) were used as witness because they are commonly used in the cultivation of L. edodes. The experimental design used was totally randomized, in 4x3 factorial scheme, totalizing 12 treatments with five repetitions, being that each repetition corresponded to a Petri dish, adding up a total of 60 dishes, which were incubated under 25 degrees C. The diameter of the colony was daily evaluated during seven days of incubation. After that period, it was verified that media prepared with P. puncticulatum extract, whose supplementation with both brans was favorable for fungic development, presented some of the highest averages of mycelial growth of LED 20 strain of L. edodes, confirming the potential of those residues.

Introdução de Lentinulaedodes (Shiitake) e Pleurotusostreatus (Shimeji) em bagaço de cana termo explodido para diminuição da toxicidade

Ethanol production has gained great prominence in the investment new renewable energy sources and Brazil is among the leaders of production. However, this activity generates large amounts of waste being the largest volume of the sugar cane bagasse. For this reason looking up ways to use this material as burning for energy production and composition of forage in the diet of ruminants, however there are difficulties to use this production for this last one. This paper proposes a microbiological treatment with Lentinula edodes and Pleurotus ostreatus in order to enable the bagasse in ruminant feed composition in order to be used more noble than their burning. After treatment with the fungus, tests were performed for quantifying crude protein by the method of Kjeldhal. It was verified that the protein content in the pure bagasse was 1.0% after fermentation the protein content was 4.2% with L.edodes and 4.9% with P. ostreatus. To evaluate the protein quality of the product fermented by L. edodes and P. ostreatus was applied microbiological method for growth of Enterococcus zimogenes verifying that after fermentation the protein quality was 76 and 27.4% with L. edodes and P.ostreatus, respectively, compared with casein. The quantification of amino acids showed significant improvement of protein with altered amino acid profile with treatments of fungos. About of DQO and BOD were also found considerable improvement besides considerable drop in toxicity as measured by acute toxicity test with Daphinia similis

Thermal and mechanical shocks affecting the first flush of production of Lentinula edodes on Eucalyptus saligna logs

O objetivo deste trabalho foi avaliar o efeito do choque térmico e mecânico na produtividade de Lentinula edodes em 140 toras de Eucalyptus saligna, completamente colonizadas pelo fungo, em diferentes tempos de imersão em água e no primeiro fluxo de produção. As toras foram imersas em água resfriada (16ºC) ou à temperatura ambiente (22ºC); os períodos de imersão corresponderam a 6, 10, 14, 18, 22, 26 e 38 horas; o choque mecânico foi acompanhado por três quedas consecutivas da tora, em posição vertical, no chão. A temperatura da água e o tempo de imersão afetaram a produção de L. edodes, resultando em aumentos significativos (2 a 4 vezes) nos tratamentos em que as toras foram submetidas à água resfriada e nos tempos de imersão mais curtos (6 e 10 horas). O choque mecânico não resultou em aumento na produção de basidiomas.

Antimutagenic effect of Lentinula edodes (BERK.) Pegler mushroom and possible variation among lineages

This study was performed to evaluate the efficiency of four different lineages (95/01, L1, 96/22 and JABK) of Lentinula edodes (BERK.) Pegler mushroom (shiitake) for inhibiting the N-ethyl-N-nitrosourea (ENU) clastogenicity in vivo. Male Swiss mice (10 animals/group) were treated during 15 consecutive days with dried mushroom added to basal diet under three different concentrations (1, 5 and 10%). At day 15, mice were intraperitoneally injected with ENU (50 mg/kg body weight) and sacrificed 24 h later for evaluation of micronucleated bone marrow polychromatic erythrocytes (MNPCE). Negative and positive controls (10 animals each), receiving basal diet and saline or ENU ip injection, respectively, were also evaluated. Results showed that pretreatments with diets containing the lineages 95/01, L1 and 96/22 reduce the frequencies of MNPCE induced by ENU. The absence of an antimutagenic activity for the lineage JABK might be related to intrinsic differences among the lineages such as biochemical composition. Taken together, our data show that the differences in protective activities of the mushrooms need to be clarified in further studies and the mechanisms for such activities need to be investigated. (C) 2003 Elsevier B.V. Ltd. All rights reserved.

Caracterização da produção em toros do cogumelo comestível Lentinula edodes (Berk.) Pegler na região oeste do Estado de São Paulo

This study was carried out in the towns of Dracena, Junquciropolis, Mirandopolis, Aliancas, Ilha Solteira, Castilho, Aracatuba, Birigui and Guararapes, São Paulo State, by surveying 17 Shiitake growers through a questionnaire. Data pertaining to the stages of log-Shiitake growing, recording and characterization of growers and growing systems were entered into Microsoft Excel for Windows. The results showed that Shiitake cultivation is recent and increasing in this region, and that growers have a high education level. Shiitake cultivation is mainly located in rural areas, with both Brazilians of Japanese descent and native Brazilians growing it. The most commonly used trees are eucalyptus and mango. The high level of log contamination is perhaps due to growing without temperature or moisture control and to the inappropriate growing system.. In 2004, there were 45,000 Shiitake-inoculated logs in this region, and the yield stood around 200 g of fresh mushroom/log. The mushrooms are picked in boxes of 200 g, and are sold mainly to Ceasa in open markets.

Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) pegler lignocellulolitic activity

This work aimed to study the stationary and periodically mixed culture of L. edodes to the production of lignocellulolitic enzymes activity. LE 95/17, LE 96/22 and Leax strains were incubated in 25 g of eucalyptus sawdust substrate in Erlenmeyer flasks in stationary culture at 25 degrees C and in a bioreactor with four complete rotations daily at 25 degrees C and 3% CO2. The samples were collected at 8, 11, 14, 17 and 20 days after the incubation. Oxidative and hydrolytic enzymes analyses were performed. Lignin peroxidase enzyme was not found in the lignolytic systernfor LE 95/17, LE 96/22 and Leax strains in the different incubation methods. The use of bioreactor could be a practicable system to induce the laccase activity for L22 and Leax and MnP activity for L17 and L22. The activity of the hydrolytic enzymes was higher in the stationary system in comparison to periodically mixed system in the bioreactor.

Atividade de enzimas oxidativas do Lentinula edodes em substratos agroindustriais

Basidyomycete Lentinula edodes has its related enzymatic activity mainly on the agribusiness waste kind used as a substrate. The objective of this work was to verify the activity of oxidative enzymes lacase (Lac), lignina peroxidase (LiP) and manganese peroxidase (MnP) of three L. edodes strains, in stationary system, cultivated the 25 degrees C, in the absence of light, in substrates wtih 20% of bran of rice, 1% of CaCO(3) and 79% of rice husk (CA), eucalyptus sawdust (SE), cassava bagasse (BM) and sugarcane bagasse (BC), adjusted to 60% of humidity. The Lac and MnP activities were bigger in eucalyptus sawdust (SE) and sugarcane bagasse (BC). The UP activity was not induced for tested substrates. The rice husk (CA) and cassava bagasse (BM) Substrates, although are not adequate to produce Lac or MnP, can be used as additives to increase the porosity, air availability and easy metabolism polysaccharides.

Influence of solid wastes in the mycelium growth of Lentinula edodes (berk.) Pegler

In Brazil there was little research related to Shiitake axenic culture. The aim of this research was to understand the substratum effects in the kinetics of the Shiitake mycelium growth. It was used two Shiitake strains and two different base substrate (eucalyptus sawdust and sugar cane bagasse) varying in three proportions of the supplements. The supplements, a blend of rice and wheat brans, were added in the proportion of 0, 10 and 20% of the base substrate. The experiment was composed of six treatments. The mycelium growth kinetics in volume had no effect relation to the strains and substrate and it followed a mathematical model represented by logarithmic equation. Beta, gamma and delta parameters didn't show any correlation with the growth velocity in volume. The strain L55 was better adapted than L17.

Desenvolvimento micelial de Lentinula edodes como efeito da profundidade e suplementação do substrato

The objective of the present study was to assess the rate of mycelium development of Lentinula edodes (Berk.) Pegler as an effect of depth and supplementation of the sugar cane bagasse substrate with different amounts of rice bran and sugar cane molasses. The experimental design consisted in a 7 × 2 factorial scheme (seven levels of bran or molasses x two growth phases) using autoclavable glass flasks to keep the substrates. The proportions of rice bran tested were: 0, 10, 15, 20, 25, 30 and 40% (dry weight/bagasse dry weight), and the concentrations of sugar cane molasses were: 0, 10, 20, 30, 40, 50 and 60 g/kg substrate. Graph paper strips externally slicked to the flask were used to measure the mycelial development. To differentiate the growth as a function of depth, the mycelial development was divided into two phases: an initial one (upper half of the flask) and a final one (lower half). The rate of mycelium formation was always higher in the early growth than in the final phase regardless of the amount of supplement. High bran proportions reduced the rate of mycelium formation, especially during the final phase, and sugar cane molasses did not affect growth rate.

Farming technology, biochemistry characterization, and protective effects of culinary-medicinal mushrooms Agaricus brasiliensis S. Wasser et al. and Lentinus edodes (Berk.) Singer: Five years of research in Brazil

The biotechnology, biochemical characterization, and protective effects of the himematsutake and shiitake mushrooms were studied for the Thematic Project, from 1998 to 2003. A new species, Agaricus Brasiliens is Wasser et al. (= A. blazei Murrill ss. Heinem.), was proposed for the cultivated lineages in Brazil. Interactions among lineages, substrates, casing layers, and fructification inductions (temperature alternations) significantly increased the productivity of A. brasiliensis in Brazil (from 40 g to 200 g of fresh mushroom kg -1 moist compost). However, pests and diseases (mainly Sciaridae flies and false truffle), drastically reduced the productivity of A. brasiliensis (below 50 g kg -1). Biochemically for each mushroom species, the polar extracts, no polars, and medium polarity presented the same organic substances; however, their concentrations differed as a result of the lineages, maturation of the fruit bodies, and cultivation conditions. In this aspect, concentration of linoleic acid in A. brasiliensis (added to animal food) was related directly to the chemical protection against carcinogenic drugs in mice. Aqueous extracts of Lentinus edodes (= Lentinula edodes (Berk.) Pegler) and A. brasiliensis may be preventive chemical protectors against mutagenic and carcinogenic drugs, depending on the lineage and extraction method (tea or juice). However, immunomodulator effects and tumor reduction were only observed with concentrated fractions (hexanic, methanolic, and ATF extracts). Aqueous extracts of A. brasiliensis and L. edodes have antibiotic-like substances and substances able to act as elicitors of resistance responses in some plants (local and systemic) and show a potential to be used in the alternative control of plant pathogens. © 2005 by Begell House, Inc.