Metodologias de pesquisa para o estudo geográfico da sociabilidade juvenil

This study aims to present the methodologies of research used to carry out the dissertation requirements related to the different times and spaces linked to the youth sociability. The methodologies are about qualitative features not at all unknown to the geography studies despite the little account taken on them. The methodologies are: eyewitness accounts, Oral History, interviews and groups of debate. With this purpose, this work intends to bring some contribution to geographers and beginners that have been looking for methodological references to their researches it also tries to facilitate the discussion on the methodological dimension in the geographical studies.

Case report: Disseminated Mycobacterium tuberculosis infection in a dog

An uncommon disseminated Mycobacterium tuberculosis infection is described in a 12-year-old female dog presenting with fever, dyspnea, cough, weight loss, lymphadenopathy, melena, epistaxis, and emesis. The dog had a history of close contact with its owner, who died of pulmonary tuberculosis. Radiographic examination revealed diffuse radio-opaque images in both lung lobes, diffuse visible masses in abdominal organs, and hilar and mesenteric lymphadenopathy. Bronchial washing samples and feces were negative for acid-fast organisms. Polymerase chain reaction (PCR)-based species identification of bronchial washing samples, feces, and urine revealed M. tuberculosis using PCR-restriction enzyme pattern analysis-PRA. Because of public health concerns, which were worsened by the physical condition of the dog, euthanasia of the animal was recommended. Rough and tough colonies suggestive of M. tuberculosis were observed after microbiological culture of lung, liver, spleen, heart, and lymph node fragments in Löwenstein-Jensen and Stonebrink media. The PRA analysis enabled diagnosis of M. tuberculosis strains isolated from organs. Copyright © 2013 by The American Society of Tropical Medicine and Hygiene.

Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil

Background: Mycobacterium spp. is one of the most important species of zoonotic pathogens that can be transmitted from cattle to humans. The presence of these opportunistic, pathogenic bacteria in bovine milk has emerged as a public-health concern, especially among individuals who consume raw milk and related dairy products. To address this concern, the Brazilian control and eradication program focusing on bovine tuberculosis, was established in 2001. However, bovine tuberculosis continues to afflict approximately 1,3 percent of the cattle in Brazil. In the present study, 300 samples of milk from bovine herds, obtained from both individual and collective bulk tanks and informal points of sale, were cultured on Löwenstein-Jensen and Stonebrink media. Polymerase chain reaction (PCR)-based tests and restriction-enzyme pattern analysis were then performed on the colonies exhibiting phenotypes suggestive of Mycobacterium spp., which were characterized as acid-fast bacilli.Results: Of the 300 bovine milk samples that were processed, 24 were positively identified as Mycobacterium spp.Molecular identification detected 15 unique mycobacterial species: Mycobacterium bovis, M. gordonae, M. fortuitum, M. intracellulare, M. flavescens, M. duvalii, M. haemophilum, M. immunogenum, M. lentiflavum, M. mucogenicum, M. novocastrense, M. parafortuitum, M. smegmatis, M. terrae and M. vaccae. The isolation of bacteria from the various locations occurred in the following proportions: 9 percent of the individual bulk-tank samples, 7 percent of the collective bulk-tank samples and 8 percent of the informal-trade samples. No statistically significant difference was observed between the presence of Mycobacterium spp. in the three types of samples collected, the milk production profiles, the presence of veterinary assistance and the reported concerns about bovine tuberculosis prevention in the herds.Conclusion: The microbiological cultures associated with PCR-based identification tests are possible tools for the investigation of the presence of Mycobacterium spp. in milk samples. Using these methods, we found that the Brazilian population may be regularly exposed to mycobacteria by consuming raw bovine milk and related dairy products. These evidences reinforces the need to optimize quality programs of dairy products, to intensify the sanitary inspection of these products and the necessity of further studies on the presence of Mycobacterium spp. in milk and milk-based products. © 2013 Franco et al.; licensee BioMed Central Ltd.

Entre os discursos, as representações e as práticas: crianças e jovens empregadas domésticas na cidade de Marília

Pós-graduação em Ciências Sociais - FFC

A máquina e o tempo: dialética das forças produtivas e do tempo de trabalho em Marx

Pós-graduação em Ciências Sociais - FFC

A articulação comunitária e o Estado: limites e possibilidades dos CONSEGs em Marília/SP

Pós-graduação em Ciências Sociais - FFC

Anti-racismo em Marília: trajetórias e perspectivas de luta do movimento negro atual

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Modernidade, medo e violência: reflexões teóricas e o caso de Marília/SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Faces de Marília: a moradia em um condomínio horizontal

Pós-graduação em Ciências Sociais - FFC

O sentido da autoridade nas destituições do poder familiar em Marília a partir dos relatos dos operadores do direito na perspectiva do gênero

Pós-graduação em Ciências Sociais - FFC

Um brinde pra mim: rivalidade e concepção de talento dos hip-hoppers de Marília

Pós-graduação em Ciências Sociais - FFC

A Igreja Universal e a política: o caso de Marília

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Amélia é quem era mulher de verdade?: mulheres pobres e a construção de uma Nova Marília (1980-2004)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produção do espaço urbano e exclusão social em Marília-SP

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Os efeitos da expansão do setor sucrolacooleiro sobre o desenvolvimento de municípios da Região Administrativa de Marília- SP

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)