Ação de pesticidas em Metarhizium anisopliae no solo

The present study aimed to analyze the action of some acaricides, fungicides, insecticides and herbicides containing different active ingredients on Metarhizium anisopliae Metsch. (Sorokin) inoculated into autoclaved soil. The action of the pesticides was evaluated based on the fungal respiratory activity. The first assessment was done at 48h after inoculation. The pesticides were then added and respiratory activity was determined nine times every 48h and an additional five times every 4 days. Except for the fungicides, no significant effect (P > 0.05) of the pesticides on M. anisopliae was observed. A reduction in CO2 production was observed for the mancozeb treatment from day 4 to day 6 of incubation, and for tebuconazol between days 4 and 6, 8 and 10, and 32 and 36. The same was observed for copper oxychloride between days 10 and 12 and 32 and 36, and for chlorothalonyl between 8 and 10, 10 and 12, and 32 and 36 days of incubation. Identical effect occurred for the acaricides abamectin and fenbutatin oxide, with a reduction in CO2 production between 20 and 24 days of incubation. The herbicides glyphosate, trifluralin and ametrin reduced the respiratory activity of the fungus between days 10 and 12, while the insecticide trichlorfon reduced respiratory activity only from 32 to 40 days of incubation. The results indicate that the toxic action of pesticides on the fungus in soil is small, suggesting that this pest control bioagent can be used in combination with pesticides without compromising its activity.

Capacidade predatória de chrysoperla externa sobre bemisia tabaci biótipo b expostas ao fungo metarhizium anisopliae

The objective of this study was to determine the influence of Metarhizium anisopliae on the predatory capacity and functional response of Chrysoperla externa fed Bemisia tabaci B biotype rearing cotton. The stock rearings of B. tabaci B biotype and C. externa were maintained in cages according to preexisting methodologies. Upon hatching, predator larvae were fed on eggs of Ephestia küehniella until their 3rd instar and then fed on whitefly nymphs in density exceeding their intake capacity (150 nymphs/individual). The nymphs were offered on cotton leaf disks (5 cm diameter), 24 hours after the treatments application following: T1: Control (water); T2: M. anisopliae at the concentration of 105 conidia cm-3; T3: 10 8 conidia cm-3 origined from Metharril® formulation of the 109 viable conidia cm-3 M. anisopliae active principle. The predatory capacity was obtained by the difference between the numbers of nymphs supplied and nymphs left. Five different nymphal densities (130, 160, 190, 220, and 250) were selected for the functional response study. The number of nymphs preyed was recorded after 24, 48, 72, and 96 hours. The M. anisopliae entomopathogenic fungi did not influence predatory capacity of C. externa which ranged from 70.6 individuals at the highest concentration to 87.1 in the control. Conclued that the C. externa predatory capacity was not affected by fungi M. anisopliae concentrations. The predator showed a type-II functional response after 24 hours, and a type-I response after 48, 72s, and 96 hours independent from fungi concentrations.

Atividade patogênica dos fungos Metarhizium anisopliae, Beauveria bassiana e Paecilomyces fumosoroseus para Chrysomya puloria (Wiedemann, 1830) (Diptera: Calliphoridae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação de metarhizium anisopliae (Metsch.) Sorok. para o controle de Fidicinoides pronoe (Cicadidae) e sua compatibilidade com produtos fitossanitários utilizados na cultura do café

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Patogenicidade e seleção de isolados de Metarhizium anisopliae (Metsch.) Sorokin e Beauveria bassiana (Bals.) Vuill. para o controle de Oryzophagus oryzae (Costa Lima, 1936) (Coleoptera: Curculionidae)

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Aplicação do fungo Metarhizium anisopliae em pastagem visando o controle do carrapato Boophilus microplus em bovinos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da patogenicidade de Beauveria bassiana e Metarhizium anisopliae, sobre os diferentes estádios de Rhipicephalus sanguineus no cão e no ambiente

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção de Metarhizium anisopliae (METSCH.) SOROK. e Beauveria bassiana (BALS.) VUILL. em diferentes substratos e efeito da radiação ultravioleta e da temperatura sobre estruturas infectivas desses entomopatógenos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Seleção e avaliação de campo de isolados de Metarhizium anisopliae (Metsch.) Sorok. para o controle da cigarrinha-da-raiz da cana-de-açúcar, Mahanarva fimbriolata (Stal,1854) (Hemiptera: Cercopidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção e eficiência de isolados de Metarhizium anisopliae (Metsch.) Sorok. no controle da cigarrinha-das-raízes da cana-de-açúcar, Mahanarva fimbriolata (Stal, 1854) (Hemiptera: Cercopidae)

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Composições contendo o isolado jab 68 do fungo entomopatogênico metarhizium anisopliae e uso das composições

Essa invenção se refere ao desenvolvimento de composições com o isolado jab 68 do fungo entomopatogânico metarhizium anisopliae, em peletes de alginato de sádio, e em óleo emulsionável e pó molhável. As composições propostas são usadas no controle da mosca-dos-chifres. As composições em óleo emulsionável e pó molhável também podem ser usadas para o controle do carrapato de bovinos rhipicephalus (boophilus) microplus ou do carrapato de cães rhipicephalus sanguineus e para controle da cigarrinha da folha (mahanarva posticata) e da raiz (mahanarva fimbriolata) da cana-de-açúcar e das pastagens (deois flavopicta).

Produção semissólida de metarhizium anisopliae e beauveria bassiana em diferentes substratos e efeito da radiação ultravioleta e da temperatura sobre propágulos desses entomopatógenos

The present study was aimed to evaluate different (semi-solid) media for the production of Metarhizium anisopliae and Beauveria bassiana propagules, and to evaluate the tolerance of these propagules to ultraviolet radiation and temperature. The experiments were performed at the Biological Control Laboratory of the Instituto Biológico at Campinas, São Paulo, Brazil. For both fungi, 6 repetitions were performed for each of the 17 treatments: corn starch, full rice, parboiled rice, type-1 rice, type-2 rice, oat flakes, canjiquinha [grits], wheat flour, raw cassava flour, yellow corn flour, special wheat flour, corn flour, corn in grains, cassava starch, soy in grains, crushed wheat, and turf. The viability analysis was done in plastic plates containing BDA. For the bioassays involving exposure to ultraviolet light and temperature, BDA was also used for viability analysis, and each treatment was exposed to the UV radiation for 0, 25 and 50 seconds, the temperature exposure being at 20, 25, 30 and 35º C. Using a Potter tower, 2 mL of fungus suspension from each treatment was inoculated to the Diatraea saccharalis caterpillars. Regarding the sporulation, the largest concentrations of M. anisopliae and B. bassiana were found for the treatments with parboiled rice, type-1 rice, type-2 rice, yellow corn flour, corn flour and crushed wheat. The viability of all treatments was superior to 94.00%. Also, the longer the duration of the exposition to the UV, the smaller the number of fertile conidia. At 35o C, a significant loss of conidia viability was observed, and all the treatments presented some level of virulence.

Aspectos de engenharia da produção do fungo entomopatogênico Metarhizium anisopliae em biorreator de bandeja

Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

Produção de conídios do fungo entomopatogênico Metarhizium anisopliae em diferentes condições de cultivo e em biorreator de bandeja

Pós-graduação em Microbiologia - IBILCE

Efeito de beauveria bassiana (bals.) Vuillemin eMetarhizium anisopliae (metsch.) sorokin nos parâmetros biológicos de trichogramma atopovirilia oatman & platner, 1983 (hymenoptera: trichogrammatidae)

Two bioassays were performed to evaluate the effect of two biopesticides based on Beauveria bassiana (Bals.) Vuillemin and Metarhizium anisopliae (Metsch.) Sorokin on the biological parameters of Trichogramma atopovirilia Oatman & Platner, 1983. In the first one, displays with S. frugiperda, 1797 eggs were dropped into the biopesticide suspension and offered to the parasitoid females for 24 hours. In the second one, parasitoid females were fed with a suspension containing honey and biopesticide suspension. In both cases, after the parasitoid death they were mantained into a humid chamber to observe fungus sporulation. The experiments were maintained in a climatized chamber at 25 +/- 1 degrees C, RH 70 +/- 10%, and photophase of 14 hours. The longevity and adult mortality, total parasitism, progeny emergency, number of individuals per egg and sexual ratio were analyzed. The biopesticides did not affect the evaluated parameters and no fungus sporulation was observed in dead females. It is possible to assert that the entomopathogenic fungi B. bassiana and M. anisopliae can be used with T. atopovirilia in IPM S. frugiperda systems.