Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain

Trypanosoma cruzi comprises a pool of populations which are genetically diverse in terms of DNA content, growth and infectivity. Inter- and intra-strain karyotype heterogeneities have been reported, suggesting that chromosomal rearrangements occurred during the evolution of this parasite. Clone D11 is a single-cell-derived clone of the T. cruzi G strain selected by the minimal dilution method and by infecting Vero cells with metacyclic trypomastigotes. Here we report that the karyotype of clone D11 differs from that of the G strain in both number and size of chromosomal bands. Large chromosomal rearrangement was observed in the chromosomes carrying the tubulin loci. However, most of the chromosome length polymorphisms were of small amplitude, and the absence of one band in clone D11 in relation to its reference position in the G strain could be correlated to the presence of a novel band migrating above or below this position. Despite the presence of chromosomal polymorphism, large syntenic groups were conserved between the isolates. The appearance of new chromosomal bands in clone D11 could be explained by chromosome fusion followed by a chromosome break or interchromosomal exchange of large DNA segments. Our results also suggest that telomeric regions are involved in this process. The variant represented by clone D11 could have been induced by the stress of the cloning procedure or could, as has been suggested for Leishmania infantum, have emerged from a multiclonal, mosaic parasite population submitted to frequent DNA amplification/deletion events, leading to a 'mosaic' structure with different individuals having differently sized versions of the same chromosomes. If this is the case, the variant represented by clone D11 would be better adapted to survive the stress induced by cloning, which includes intracellular development in the mammalian cell. Karyotype polymorphism could be part of the T. cruzi arsenal for responding to environmental pressure. © 2013 Lima et al.

Gestão do desempenho organizacional em empresas do setor de construção civil: uma aplicação do Balanced Scorecard

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aplicação do balanced scorecard como sistema de execução da estratégia e a controladoria como unidade gestão dos controles operacionais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Comparison of oxidative stress in ASA physical status I patients scheduled for minimally invasive surgery under balanced or intravenous anesthesia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Capturing Transition Paths and Transition States for Conformational Rearrangements in the Ribosome

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Balanced anesthesia with sevoflurane does not alter redox status in patients undergoing surgical procedures

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo da utilização do balanced scorecard na gestão da qualidade e excelência operacional aplicadas a projetos de engenharia em sistemas de potência

The expansion of the electrical system is currently a subject of great importance to the development of Brazil. For its realization, large projects are needed, requiring large-scale investment and realization processes of great complexity. Thus, this type of project has a high strategic importance for the performance of companies that conduct. Seeking a way to determine a method to align traditional practices for measuring quality to the corporate strategic needs, this work is the study of the use of the balanced scorecard methodology, applying quality management and operational excellence, with a focus on projects in power systems. We analyzed some cases of successful application of the methodology in business segments in the next study, culminating in a proposed model of the method

Análise da implementação do balanced scorecard em uma fornecedora de tecnologia da informação

Many companies have invested substantial resources in recent years in implementing the Balanced Scorecard, a strategic management tool that embraces not only financial perspectives, but also three other perspectives that create an overall and long-term vision of the company. The purpose of this article is to analyze, through interviews and action research, the implementation of the Balanced Scorecard in a provider of information technology and suitability for the company area Alliances and Channels, identifying determinants factors that exist in the literature and contributing to a greater understanding around this strategic management tool. The study shows that the implementation of the company was not successful, but the area of Alliances and Channels suit tool in order to find good results and two new determinants, other than those existing in the literature, were found

Proposta de aplicação do Balanced Scorecard para o operador de transporte ferroviário

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)