Genotoxic evaluation of the antimalarial drugs artemisinin and artesunate in human HepG2 cells and effects on CASP3 and SOD1 gene expressions

The malaria treatment recommended by the World Health Organization involves medicines derived from artemisinin, an active compound extracted from the plant Artemisia annua, and some of its derivatives, such as artesunate. Considering the lack of data regarding the genotoxic effects of these compounds in human cells, the objective of this study was to evaluate the cytotoxicity and genotoxicity, and expressions of the CASP3 and SOD1 genes in a cultured human hepatocellular liver carcinoma cell line (HepG2 cells) treated with artemisinin and artesunate. We tested concentrations of 2.5, 5, 7.5, 10, and 20 μg/mL of both substances with a resazurin cytotoxicity assay, and the concentrations used in the genotoxicity experiments (2.5, 5, and 10 μg/mL) and gene expression analysis (5 mg/mL) were determined. The results of the comet assay in cells treated with artemisinin and artesunate showed a significant dosedependent increase (P < 0.001) in the number of cells with DNA damage at all concentrations tested. However, the gene expression analysis revealed no significant change in expression of CASP3 or SOD1. Our data showed that although artemisinin and artesunate exhibited genotoxic effects in cultured HepG2 cells, they did not significantly alter expression of the CASP3 and SOD1 genes at the doses tested. ©FUNPEC-RP.

A documentação pedagógica como processo de investigação e reflexão na educação infantil

Pós-graduação em Educação - FFC

Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Human-calf relationship and its implications on animal welfare

Pós-graduação em Zootecnia - FCAV

Human dental enamel and dentin structural effects after Er:yag laser irradiation

Ideally projected to be applied on soft tissues, infrared lasers were improved by restorative dentistry to be used in hard dental tissues cavity preparations-namely enamel and dentin. This paper evidentiates the relevant aspects of infrared Erbium laser's action mechanism and its effects, and characterizes the different effects deriving from the laser's beams emission. The criteria for use and selection of optimal parameters for the correct application of laser systems and infuence of supporting factors on the process, such as water amount and its presence in the ablation process, protection exerted by the plasma shielding and structural factors, which are indispensable in dental tissues cavity preparation related to restorative technique, are subordinated to optical modifcations caused by the interaction of the energy dissipated by these laser light emission systems in the targeted tissue substrate. Clinical relevance: Differences in the action of infrared Erbium laser system in regard to the nature of the ablation process and variations on the morphological aspects observed in the super-fcial structure of the target tissue irradiated, may be correlated to the structural optical modifcations of the substrate produced by an interaction of the energy propagated by laser systems.

Cytotoxic Effects of Zoledronic Acid on Human Epithelial Cells and Gingival Fibroblasts

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

miRNA expression in human lung cancer and fetal lung: a comparative study

Trabalho apresentado na São Paulo Advanced School of Comparative Oncology, Águas de São Pedro, SP, 2012.

A escola na transição para o digital: desafios para a incorporação de TIC e a contribuição da TVD

Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

Professores iniciantes: marcas de alteridade na constituição da profissionalidade docente

Pós-graduação em Educação - IBRC

O consumo de bebidas alcoólicas por estudantes do ensino médio e características do grupo de pares

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Nas redes da Àte: A hybris de Xerxes em Os persas de Ésquilo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Práticas corporais alternativas: um caminho para a formação em educação física

Pós-graduação em Ciências da Motricidade - IBRC

Sistema de monitoramento de temperatura e umidade em silos graneleiros via rádio

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Violência conjugal: materialização das ações profissionais na área da saúde

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Contação de histórias e educação crítica: uma proposta didática

This proposal offers a possibility of approximation of mathematical knowledge at the prospect of Critical Education defended by researchers such as Ole Skovsmose and Paulo Freire. Using storytelling as a methodological resource is intended to develop an activity that allows students to think critically about real-life situations that have democratic questions as ground. The research is part of the branch of mathematics concerned with the education goals of teaching mathematics in basic education. We discuss the relevance of an educational process that enables the development of a democratic competence in the first years of primary education by showing students that their classroom can and should be seen as a community, where everyone has rights and duties. The interpretation and understanding of real situations can be something very complex and uninteresting for students of that age, looking for an attractive feature such as storytelling, which is based on the tendency of Reading and Writing in Mathematics, proposed by Nacarato and Lopes; Cardoso and Fonseca. Adopting a phenomenological attitude in the research seeks to understand if the storytelling can contribute to the formation of the critical student. Assuming this perspective has developed a didactic sequence from the story 'o que os olhos não vêem' from writer Ruth Rocha. The student's writing, favored by the proposed sequence, subsidizes the understanding of their thinking and acting on the democratic situations