87 resultados para Histologia comparada
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Este estudo visou verificar o efeito da sonoforese com Arnica montana sobre a fase inflamatória aguda de uma lesão muscular. Para isso, 40 ratos Wistar machos, lesados cirurgicamente, foram divididos em 4 grupos: controle (C), 10 ratos lesados e não tratados; grupo ultra-som (US), 10 lesados, tratados com US; grupo ultra-som com arnica (US+A), 10 ratos lesados, tratados com sonoforese de gel de arnica; grupo arnica (A), 10 ratos lesados, tratados com massagem de gel de arnica. O tratamento dos três grupos foi iniciado 24h após a lesão, sendo aplicado uma vez ao dia durante 3 minutos, por três dias. Quatro dias após a lesão, os animais foram sacrificados e o terço médio do músculo tibial anterior lesado foi removido e tratado histologicamente. Os resultados da análise qualitativa mostram que, no grupo C, formou-se um intenso infiltrado de células inflamatórias no espaço intersticial e um processo de regeneração apenas iniciado. Nos grupos US e US+A foi detectado um avançado processo inflamatório, com tecido conjuntivo mais organizado e consistente. No grupo A foi detectada diminuição no número de células inflamatórias e uma desorganização em sua disposição, o que poderia levar a um atraso no processo de regeneração. Conclui-se que os grupos que receberam a aplicação do ultra-som e ultra-som com arnica apresentaram semelhante aceleração do processo inflamatório agudo, sugerindo ineficácia da sonoforese quando comparada à aplicação de apenas ultra-som.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Anatomia comparada do pulvino primário de leguminosas com diferentes velocidades de movimento foliar
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Histologia da associação de membranas biológicas de origem bovina implantadas no subcutâneo de ratos
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O trabalho teve por objetivo avaliar a capacidade de barreira da membrana de cortical óssea desmineralizada liofilizada bovina, testando sua permeabilidade e integridade, em associação com a membrana de pericárdio bovino liofilizado. em 15 ratos Wistar, machos, adultos, implantou-se no subcutâneo da região cérvico-torácica dorsal a combinação de duas membranas de cortical óssea desmineralizada tendo no interior a membrana de pericárdio bovino. Os animais (cinco ratos/grupo) foram submetidos à eutanásia aos 15, 30 e 60 dias de pós-operatório. A avaliação microscópica mostrou que, aos 15 dias de pós-cirúrgico, as membranas estavam íntegras, com exceção de pequenas áreas de reabsorção da membrana de cortical óssea junto aos locais referentes aos antigos canais nutritivos. Aos 30 dias, havia apenas restos da membrana de cortical óssea e, aos 60 dias, a sua total ausência. Baseados nos resultados aqui obtidos, é possível concluir que a membrana de cortical óssea bovina é rapidamente absorvida e não confere proteção à membrana de pericárdio bovino liofilizado.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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The objective of this study was to evaluate the in vitro activity of cefepime, cefpirome and amikacin against the most prevalent nosocomial bacteria. Initially a prospective study was designed to compare the bacterial susceptibility to the three drugs using 1,022 pathogenic strains. The strains were isolated from hospitalized patients of the Hospital das Clinicas - Faculdade de Medicina de Botucatu, SP, from March to December of 1996, by using the Bauer-Kirby susceptibility diffusion controlled method. The activity of cefepime by the Kirby-Bauer method was significantly higher (χ2, p ≤ 0.05) than cefpirome and amikacin for the following bacteria: P. aeruginosa (72% x 56% x 64%, respectively), Enterobacter cloacae (98% x 88% x 80%) and total strains (79.5% x 74.3% x 76.8%). Cefpirome exhibited higher activity than cefepime only to Enterococcus faecalis (42% x 23%). In the 12 other bacterial groups studied the sensibility of the three drugs was similar (χ2, p ≥ 0.05). The minimal inhibitory concentration (MIC) for 127 bacterial strains - Enterobacter cloacae (12), Citrobacter sp (15), Pseudomonas aeruginosa (50), Acinetobacter baumannii (12), BGNF others (22) and Enterococcus faecalis (16)-from the same origin previously described and isolated during 1997, was determined by E-test. Ranges of MIC intervals, MIC(50%), MIC(90%) and the proportion of the sensitive bacterial strains were determined and permitted the following analysis: the activity of cefepime against Gram-negative bacteria was 2 or more times higher than that of cefpirome and amikacin, specially when CIM(90%) was considered; the activity of cefpirome was higher only against E. faecalis. This information must be considered in the rational use of antibiotic, specially in patients with nosocomial infections.
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With the development of greenhouse grown crops, the whitefly Trialeurodes vaporariorum (West.) has become an important pest. Aiming at verifying the effect of host on the development of this species, the biology of the whitefly was studied on bean (Phaseolus vulgaris L.) cultivars IAC-Carioca Pyatã, IAPAR-57, Jalo Precoce, IAC-Bico de Ouro, IAC-Maravilha and on soybean (Glycine max L.) cultivar Cristalina. The study was conducted at the Laboratório de Entomologia Agrícola - FCA-UNESP, at 25 ± 3°C temperature, 80 ± 10% RH and 14h photophase. The biological parameters assessed were duration, viability and number of nymphal instars; male and female longevity, oviposition period, number of eggs per female and sex ratio; egg viability and incubation period and the life cycle duration. Based on data obtained, fertility life tables were constructed to allow a comparative analysis of the effect of cultivars on the biological development and consequent population growth of this species. The bean cultivars IAC-Maravilha and Jalo Precoce negatively influenced T. vaporariorum development, whereas, the other bean cultivars studied, as well as the soybean cultivar Cristalina, provided good conditions for development of this pest.
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This research was aimed at verifying the occurrence of possible alterations in liver, kidney and intestine of bullfrog tadpoles in stage 42 fed with diets containing different concentrations (0.0; 0.2; 0.5; 1.0 and 1.5%) of propolis hydroalcoholic extract. The experiment was carried out in laboratory of Aquatic Organisms Nutrition from Aquaculture Center of UNESP, where 1,400 tadpoles in stage 26 were used and distributed in twenty experimental aquariums. In the end of experiment (60 days) three tadpoles from each repetition were sacrificed and kidney, liver and intestine samples were collected to processing of histological slices in Histology's Laboratory pertaining to Department of Morphology and Physiology from FCAV - UNESP. Samples were fixed, dehydrated, stained in HE, analysed, photomicrographed and thickness of intestinal epithelium was measured. Histological disturbances in tadpoles's intestine, kidneys and liver were not observed. Thickness of intestinal epithelium from the same ones was not influenced (P>0.05) by propolis concentrations.
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A comparative evaluation was made of the use of natural language versus two specialized indexing languages, aiming to demonstrate the influence of the availability of indexing languages on the functioning of information retrieval systems. The study was conducted within the ambit of the construction of search strategies by subject in online university library catalogs. The precision ratio was calculated to determine the accuracy of each indexing language in subjectbased information retrieval. From the comparative evaluation of the use of indexing languages, it was concluded that the term specificity required by the user during retrieval was more satisfactory when the query was made through controlled languages, whose availability and simplicity is also an indispensable requisite.