Small-angle X-ray scattering and structural modeling of full-length: Cellobiohydrolase I from Trichoderma harzianum

Cellobiohydrolases hydrolyze cellulose releasing cellobiose units. They are very important for a number of biotechnological applications, such as, for example, production of cellulosic ethanol and cotton fiber processing. The Trichoderma cellobiohydrolase I (CBH1 or Cel7A) is an industrially important exocellulase. It exhibits a typical two domain architecture, with a small C-terminal cellulose-binding domain and a large N-terminal catalytic core domain, connected by an O-glycosylated linker peptide. The mechanism by which the linker mediates the concerted action of the two domains remains a conundrum. Here, we probe the protein shape and domain organization of the CBH1 of Trichoderma harzianum (ThCel7A) by small angle X-ray scattering (SAXS) and structural modeling. Our SAXS data shows that ThCel7A linker is partially-extended in solution. Structural modeling suggests that this linker conformation is stabilized by inter- and intra-molecular interactions involving the linker peptide and its O-glycosylations. © 2013 Springer Science+Business Media Dordrecht.

A dimensão política da educação ambiental: reflexões sobre os trabalhos dos encontros de pesquisa em Educação Ambiental (I, II, III EPEAS)

Pós-graduação em Educação - IBRC

Tradição e reinvenção: as convergências em I nostri antenati fr Italo Calvino e Primeiras estórias de João Guimarães Rosa

Pós-graduação em Letras - IBILCE

Aprendizagem de alunos com deficiência intelectual do ciclo I do Ensino Fundamental: o desempenho em leitura e escrita e a formação de professores

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

The Epstein-Glaser causal approach to the light-front QED(4). I: Free theory

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Coordinative versatility of a Schiff base containing thiophene: Synthesis, characterization and biological activity of zinc(II) and silver(I) complexes

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Specific Activation of an I-Like Element in Drosophila Interspecific Hybrids

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Expresividad poética y traducción con breve lectura de Fedro I. 1

The term poetic expressiveness refers to the multiple joints of the plan of expression, derived from the expressive value of the linguistic sign (ROSSET: 1970, 135) and its particular role in the field of poetry. The features of meaning, such as projection, elevation and salience, make it possible to consider expressive all poetic statements which constitute particularly dense instances in the formal consolidation of a convergence between the two planes (expression/content), and therefore it stands out from the others due to the high density of structural parallelisms and isomorphisms, which are procedures responsible for the impression that a particular form of content can only be expressed by cutting that same specific form of expression out. These considerations have an immediate impact on the reading, interpretation and practice of translating poems, which is intended to be demonstrated here, through an example of translation of a Phaedrus' fable, written in iambic meter.

Os elementos religiosos nas sátiras do poeta Juvenal (séculos I e II d.C)

Pós-graduação em História - FCLAS