Metabolismo energético do camarão da amazonia, Macrobrachium amazonicum

Pós-graduação em Biologia Geral e Aplicada - IBB

Análise da variabilidade genética e do padrão de atividade esterásica no hepatopâncreas de camarões palemonídeos expostos a diferentes pesticidas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do potencial bioindicador de Trichdactylus fluviatilis (Latreille, 1828) (Crustaceae : Decapoda : Trichodactylidae) na bacia do Rio Corumbataí

Pós-graduação em Ciências Biológicas (Zoologia) - IBRC

Aspectos estruturais do hepatopâncreas, desenvolvimento ovocitário e caracterização hormonal de fêmeas de Macrobrachium amazonicum durante as fases de maturação gonadal

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caracterização estrutural do sistema reprodutor masculino e do hepatotâncreas dos diferentes morfotipos de Macrobrachium amazonicum

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Farfantepenaeus brasiliensis (Crustacea : Penaeoidea): morfologia do hepatopâncreas e sua relação com os metais pesados encontrados no litoral sul do Espírito Santo

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Qualidade da água e histopatologia de órgãos de peixes provenientes de criatórios do município de Itapecuru Mirim, Maranhão

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Bioaccumulation of tributyltin by blue crabs

This study evaluated the bioaccumulation of tributyltin (TBT) by the blue crab (Callinectes sapidus). Animals were fed with contaminated food containing 30 µg g-1 of TBT expressed as Sn. The analytes were determined in the gills, hepatopancreas and muscle. Acid digestion was used in the total Sn determination, and a solid-phase extraction technique was used for the selective determination of TBT. Limits of detection of 44.6 and 4.46 ng g-1 were found for HG-ICP OES (hydride generation-inductively coupled plasma optical emission spectroscopy) and ICP-MS (ICP-mass spectrometry), respectively. The results for non-contaminated animals were below 50 ng g-1, while the animals subjected to the contaminated food showed higher tissue concentrations of Sn (until 6229 ng g-1) and TBT (until 3357 ng g-1) related to the number of exposure days. According to the results, Sn is bioaccumulated by the blue crab in higher concentrations in the hepatopancreas. For most of these animals, the results suggest that Sn is bioaccumulated as TBT.

Development of the male reproductive system in Callinectes ornatus Ordway, 1863 (Brachyura: Portunidae)

This study describes the histology and histochemistry of the male reproductive system in Callinectes ornatus, comparing juvenile and adult developmental stages. We also analyzed changes in the gonadosomatic (GSI) and hepatosomatic (HSI) indices, and the weights of the testis and vas deferens during the development. The results showed that all stages, beginning with the juvenile (JUV), through developing (DEV) and mature (MAT) adult males of C. ornatus produce sperm and spermatophores. During development, testicular lobes showed the same characteristics of production and release of sperm into the seminiferous duct. The vas deferens showed little histological and histochemical change in the epithelium in juvenile and adult males. The differences consisted of the larger amount of secretion in MAT males compared to JUV and DEV ones. The chemical composition of the seminal fluid was similar, but MAT males produced a more homogeneous secretion. Morphological and physiological maturation are not synchronized in C. ornatus, since JUV males produced spermatophores similar to those in DEV and MAT males. However, these JUV are not yet able to reproduce, since they still have the abdomen attached to the cephalothoracic sternum. The increase of the GSI during development was significant for MAT males, and is related to the production of sufficient volume of seminal fluid to form the sperm plug in the female seminal receptacle. The HSI decreased from DEV to MAT adult stages, indicating that reserves from the hepatopancreas are used to develop the reproductive system after the pubertal molt.

DIGESTIVE PROTEASES FROM WILD AND FARMED MALE MORPHOTYPES OF THE AMAZON RIVER PRAWN (MACROBRACHIUM AMAZONICUM)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Dinâmica da vitelogênese durante a maturação ovariana em Artemesia longinaris

Pós-graduação em Aquicultura - FCAV

EFFECT OF FOOD SHORTAGE ON GROWTH, ENERGETIC RESERVES MOBILIZATION, AND WATER QUALITY IN JUVENILES OF THE REDCLAW CRAYFISH, CHERAX QUADRICARINATUS, REARED IN GROUPS

The aim of this study is to analyze the impact of food shortage on growth performance, by means of energetic reserves (proteins, glycogen and lipids) mobilization and hepatopancreas cells analysis in C. quadricarinatus juveniles maintained in groups, as well as the effect on culture water quality. Two experiments were performed, each of them with two feeding regimes during 45 days. The Control feeding regime, in which crayfish were fed daily (once a day) throughout the experimental period (DF), and the Cyclic feeding regime, in which juveniles were fed for 2 or 4 days (once a day) followed by 2 or 4 days of food deprivation (2F/2D and 4F/4D, respectively) in repeated cycles. Cyclic feeding influenced growth, biochemical composition from hepatopancreas and muscle, and water quality. Juveniles cyclically fed were unable to maintain a normal growth trajectory during 45 days. Apparent feed conversion ratio, apparent protein efficiency ratio, hepatosomatic index and relative pleon mass were similar in cyclic and daily fed animals and no structural damage was found in the hepatopancreas of juveniles subjected to cyclic feeding. The novelty of this study was the significant accumulation of proteins in pleonal muscle in both cyclic feeding regimes (approx. 18%) suggesting that the storage of this constitutive material during food shortage may be an adaptation for a compensatory growth when food becomes abundant again. The cyclic feeding regimes had a positive effect on water quality decreasing inorganic nitrogen concentration. This was due to the reduction in the amount of animal excretes and feces in the group that received approx. 50% less feed. Additionally, water pH was higher in cyclic feeding tanks, as a result of lower organic matter decomposition and consequent release of CO2. Accordingly, total ammonia in the water was significantly lower for the cyclic feeding regimes compared to their respective controls. This study suggests that the protocol of cyclic feeding could be applied at least 45 days in 1 g juveniles maintained in group conditions, without affecting the energetic reserves and hepatopancreas structure, emphasizing the high tolerance of this species to food restriction.

Análises isoenzimáticas, moleculares e ultraestruturais em camarões dulcícolas do gênero Macrobrachium

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência da temperatura nas respostas aos biocidas diazinon e carbaril em Dilocarcinus pagei Stimpson, 1861

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)