Screening of genomic libraries

Microsatellites, or simple sequence repeats (SSRs), have proven to be an important molecular marker in plant genetics and breeding research. The main strategies to obtain these markers can be through genomic DNA and from expressed sequence tags (ESTs) from mRNA/cDNA libraries. Genetic studies using microsatellite markers have increased rapidly because they can be highly polymorphic, codominant markers and they show heterozygous conserved sequences. Here, we describe a methodology to obtain microsatellite using the enrichment library of DNA genomic sequences. This method is highly efficient to development microsatellite markers especially in plants that do not have available ESTs or genome databases. This methodology has been used to enrich SSR marker libraries in Citrus spp., an important tool to genotype germplasm, to select zygotic hybrids, and to saturate genetic maps in breeding programs. © Springer Science+Business Media, LLC 2013.

Aspectos técnicos e econômicos da poda e do controle químico de Brevipalpus phoenicis no manejo da leprose dos citros

The objective of this study was to evaluate different citrus leprosis management tactics during seven seasons, based in pruning and acaricide applications, considering technical and economic aspects of each tactic. The trial was conducted from October 2003 to August 2010 in an orange plantation of Pera cv. located in the municipality of Reginópolis-SP, Brazil. The plants of citrus used were12 years old and grafted onto Cleopatra tangerine. The experimental design used was randomized blocks, in a factorial scheme, made up of factors type of pruning (A), with six levels: (1) drastic pruning, (2) intermediate pruning without leprosis lesions, (3) intermediate pruning with leprosis lesions (4) light pruning, (5) without pruning and (6) replant; acaricide applications factor (B), with three levels: (1) without acaricides applications, (2) with lime sulfur applications and (3) spirodiclofen or cyhexatin applied in rotation; pruning factor to remove leprosis symptomatic branches (C), with two levels: (1) with pruning for removal, (2) without removal pruning. The combination of factors, with respective levels (6 x 3 x 2), resulted in 36 treatments that were repeated four times, with each parcel being made up of three plants in a row. After seven years, it was observed that the types of the pruning and remove of leprosis symptomatic branches used as single management tactic, is not sufficient to leprosis control. Therefore, the results demonstrated that for leprosis management, it is essential the association between tactics, especially the control mite vector. For ensuring the citrus production economically, the use of acaricides highly efficient is essential in B. phoenicis control. The recommendation of the type of pruning should be in function of the leprosis incidence and severity in the orchard. In orchards with low leprosis incidence and severity the light pruning is more appropriate, because it is efficient and ensures greater financial balance. However, in orchards with high leprosis incidence and severity it is indicated the severe pruning for reducing or eliminating inoculum source. Between the severe prunings, the intermediate pruning with leprosis lesions is the most relevant, because the financial return will be faster. Replant is indicated only for young orchards, because does not imply in changes onto cultural practices.

Efeitos de óleos nas características físicas e químicas da calda de aplicação e na ação da atrazina

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Determinação de ácido abscísico e respostas metabólicas em espécies congenéricas de savana e mata do cerrado paulista

Pós-graduação em Ciências Biológicas (Biologia Vegetal) - IBRC

Vírus da hepatite B: avaliação do risco de infecção e da resposta sorológica à vacina em funcionários de limpeza do Hospital das Clínicas da Faculdade de Medicina de Botucatu - UNESP

Pós-graduação em Doenças Tropicais - FMB

Propriedades físico-químicas de vidrados cerâmicos preparados com rejeitos galvânicos atuando como corantes

Pós-graduação em Física - IGCE

Caracterização físico-química, microbiológica e planctônica de um viveiro coberto por macrófitas usado no abastecimento de água para aquicultura

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização molecular e biológica do Lettuce big-vein associated vírus e Mirafiori lettuce big-vein vírus e estudo da ocorrência em relação à época e sintoma em plantas de alface no Estado de São Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Oxidação da hemoglobina como modelo de estudo do sistema de óxido-redução eritrocitário: interação entre nitrito de sódio, azul de metileno e cistamina

Pós-graduação em Fisiopatologia em Clínica Médica - FMB

Geologia, petrografia, geoquímica, comportamento físico-mecânico e alterabilidade das rochas ornamentais do stock granítico Serra do Barriga, Sobral (CE)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caracterização citogenética e molecular das espécies Cichla kelberi e Cichla piquiti e seu possível híbrido interespecífico coletados em ambientes naturais

Pós-graduação em Ciências Biológicas (Zoologia) - IBB

Light-Induced Structural Change in Iridium Complexes Studied by Electron Spin Resonance

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Morte e morrer: opinião dos profissionais de saúde de uma unidade de terapia intensiva pediátrica

Presently, the dying process and death most often occur in hospitals and, particularly, in Intensive Care Units (ICU), where patients’ lives are prolonged thanks to advanced technological devices and highly efficient medicines. To learn about the opinion of health care professionals working at a Pediatric Intensive Care Unit in relation to the dying process and dying. This is a descriptive quantitative study. A questionnaire was applied to the unit’s staff members from June to August, 2011. Data were statistically analyzed. Twenty-five professionals answered the questionnaire, and 72% faced death as a natural life process. 60% felt compassion, but that feeling did not interfere with how they cared for patients. Concerning their professional training, 52% reported not to have received any concerning patients’ caregivers in the dying process or death; therefore, they experienced such situation when they were already working, and 76% reported to be interested in updating courses on that theme. Further discussion about this topic during academic education is necessary. It is also necessary to provide health care professionals with specialization courses, debates and experience exchange so that they can better understand and deal with their feelings and limitations in face of death and thus give better care to patients and relate to patients’ families during the dying process of a loved one

Desenvolvimento, implantação e avaliação do desempenho de biorreator anaeróbico de leito fluidizado e leito fixo com filtros biológicos em materiais diversos

This study aimed to develop, implement and evaluate the performance of a new type of bioreactor for anaerobic treatment of wastewater using different filling materials like trickling filters post-reactor. This bioreactor has mixed characteristics of the UASB reactors and horizontal flow from the point of view of removal of BOD (Biochemical Oxygen Demand) ssed (settled solids), TS (Total Solids), SS (Suspended Solid), SD (Dissolved Solids) and turbidity. The experimental model consists of a bioreactor with a volume of 12 m³, 2/3 filled by fluidized bed and 1/3 for fixed. The fluidized bed is made of polystyrene plates used as a system percolation and compartmentalized trickling filters, where each compartment was filled with a support medium with different characteristics (gravel number 4, plastic rings of polystyrene, PET and HDPE) . In addition, the output of a filter system was installed three entries filled with activated carbon. The bioreactor was installed in private residence in the city of Igarapava-SP (20° 02'40.18"S and 47° 45'01.36" W). The system was highly efficient as the removal of organic contaminant load 92% on average reducing the BOD, a significant result when compared to other anaerobic systems. For the other parameters, the mean reduction was 96% for turbidity, 99% ssed, 67.5% ST, 57% SD and 88% of SS. As for its operation the system was capable of operating in continuous flow without the need for maintenance during the entire period of evaluation and without energy, as it operates taking advantage of the natural slope of the terrain where it is installed. The environmental impacts were minimized due to the preservation of local vegetation allowing the ecosystem to remain unchanged beyond the prototype was completely sealed preventing exhalation of odors and therefore not causing inconvenience to neighboring populations. Given these facts it was concluded that the prototype is shown to be highly feasible deployed as a new alternative for treatment of sewage in rural and urban settings (individual homes, condos, farms, ranches, etc.) Due to ease of design and operability, and sustainability at all stages of execution.

Mortalidade e efeitos subletais de Bacillus thuringiensis Berliner em Spodoptera albula (Walker, 1857)

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV