Dynamics of bone healing after osteotomy with piezosurgery or conventional drilling - histomorphometrical, immunohistochemical, and molecular analysis

Background: Piezosurgery is an osteotomy system used in medical and dental surgery. Many studies have proven clinical advantages of piezosurgery in terms of quality of cut, maneuverability, ease of use, and safety. However, few investigations have tested its superiority over the traditional osteotomy systems in terms of dynamics of bone healing. Therefore, the aim of this study was to evaluate the dynamics of bone healing after osteotomies with piezosurgery and to compare them with those associated to traditional bone drilling.Methods: One hundred and ten rats were divided into two groups with 55 animals each. The animals were anesthetized and the tibiae were surgically exposed to create defects 2 mm in diameter by using piezosurgery (Piezo group) and conventional drilling (Drill group). Animals were sacrificed at 3, 7, 14, 30 and 60 days post-surgery. Bone samples were collected and processed for histological, histomorphometrical, immunohistochemical, and molecular analysis. The histological analysis was performed at all time points (n = 8) whereas the histomorphometrical analysis was performed at 7, 14, 30 and 60 days post-surgery (n = 8). The immunolabeling was performed to detect Vascular Endothelial Growth Factor (VEGF), Caspase-3 (CAS-3), Osteoprotegerin (OPG), Receptor Activator of Nuclear Factor kappa-B Ligand (RANKL), and Osteocalcin (OC) at 3, 7, and 14 days (n = 3). For the molecular analysis, animals were sacrificed at 3, 7 and 14 days, total RNA was collected, and quantification of the expression of 21 genes related to BMP signaling, Wnt signaling, inflammation, osteogenenic and apoptotic pathways was performed by qRT-PCR (n = 5).Results: Histologically and histomorphometrically, bone healing was similar in both groups with the exception of a slightly higher amount of newly formed bone observed at 30 days after piezosurgery (p < 0.05). Immunohistochemical and qRT-PCR analyses didn't detect significant differences in expression of all the proteins and most of the genes tested.Conclusions: Based on the results of our study we conclude that in a rat tibial bone defect model the bone healing dynamics after piezosurgery are comparable to those observed with conventional drilling. © 2013 Esteves et al.; licensee BioMed Central Ltd.

Desempenho de cultivares transgênicas de soja em sucessão a culturas de inverno em semeadura direta

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A fuga dupla luso-brasileira durante os séculos XVIII e XIX

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A busca do ser e da arte em O Cavaleiro Inexistente de Ítalo Calvino

Pós-graduação em Letras - IBILCE

Anita Garibaldi coberta por histórias

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O experimento investigativo e representações de alunos de ensino médio: obstáculos epistemológicos em questão

Este trabalho teve por finalidade explorar o potencial teórico-metodológico de uma atividade investigativa como recurso didático para o processo de construção e validação de hipóteses. Objetivou-se, também, analisar as interpretações discursivas - representações - produzidas pelos alunos quando se deparam com fenômeno(s) que não consegue(m) explicar, ou explicam utilizando argumentos distorcidos do ponto de vista científico. Nesta perspectiva, as representações dos alunos foram discutidas tendo em conta a presença de obstáculos epistemológicos bachelardianos. Os resultados mostram que nessas representações aparecem os obstáculos animistas e realistas, os quais podem dificultar a compreensão dos conceitos científicos por parte dos alunos. Apenas ocasionalmente aparece o obstáculo verbalista, relacionado com o uso de uma linguagem comum para explicar certo conceito científico. O obstáculo substancialista também se faz presente por meio da “propriedade substancial” responsável por algumas distorções conceituais produzidas pelos alunos.

Anita Garibaldi coberta por histórias

Onde termina o mito e começa a mulher? Se a vida repleta de aventuras de Anita Garibaldi já deu origem a vários filmes e minisséries, também é tema recorrente na literatura não apenas brasileira, mas até na hispano-americana. No livro Anita Garibaldi coberta por histórias, Fernanda Aparecida Ribeiro analisa com elegância e concisão as imagens construídas da revolucionária catarinense por dois escritores brasileiros - João Felício dos Santos e Flávio Aguiar - e dois argentinos - Julio A. Sierra e Alicia Dujovne Ortiz. Ribeiro discute o esforço desses autores de contextualizarem melhor o mito dominante em torno de Anita - o da heroína de dois mundos, que lutou por seus ideais na América do Sul e na Itália. E também a tentativa que eles fizeram para se desvencilhar da outra imagem, mais domesticada, da companheira de Giuseppe Garibaldi, vista antes como esposa e mãe do que como revolucionária por vários historiadores brasileiros, principalmente no começo do século 20.

A proposta de ensino por investigação e o processo de formação inicial de professores de ciências: reflexões sobre a construção de um modelo didático pessoal

Pós-graduação em Educação para a Ciência - FC

Adubação nitrogenada, fosfatada e potássica na cultura do eucalipto (clone i144 - E. urograndis)

Pós-graduação em Agronomia - FEIS

Cardiovascular risk and mortality in end-stage renal disease patients undergoing dialysis: sleep study, pulmonary function, respiratory mechanics, upper airway collapsibility, autonomic nervous activity, depression, anxiety, stress and quality of life: a prospective, double blind, randomized controlled clinical trial

Background: Chronic kidney disease (CKD) is one of the most serious public health problems. The increasing prevalence of CKD in developed and developing countries has led to a global epidemic. The hypothesis proposed is that patients undergoing dialysis would experience a marked negative influence on physiological variables of sleep and autonomic nervous system activity, compromising quality of life.Methods/Design: A prospective, consecutive, double blind, randomized controlled clinical trial is proposed to address the effect of dialysis on sleep, pulmonary function, respiratory mechanics, upper airway collapsibility, autonomic nervous activity, depression, anxiety, stress and quality of life in patients with CKD. The measurement protocol will include body weight (kg); height (cm); body mass index calculated as weight/height(2); circumferences (cm) of the neck, waist, and hip; heart and respiratory rates; blood pressures; Mallampati index; tonsil index; heart rate variability; maximum ventilatory pressures; negative expiratory pressure test, and polysomnography (sleep study), as well as the administration of specific questionnaires addressing sleep apnea, excessive daytime sleepiness, depression, anxiety, stress, and quality of life.Discussion: CKD is a major public health problem worldwide, and its incidence has increased in part by the increased life expectancy and increasing number of cases of diabetes mellitus and hypertension. Sleep disorders are common in patients with renal insufficiency. Our hypothesis is that the weather weight gain due to volume overload observed during interdialytic period will influence the degree of collapsibility of the upper airway due to narrowing and predispose to upper airway occlusion during sleep, and to investigate the negative influences of haemodialysis in the physiological variables of sleep, and autonomic nervous system, and respiratory mechanics and thereby compromise the quality of life of patients.

Adubação fosfatada e épocas de cultivo na composição química de sementes de cultivares de feijoeiro

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Dry matter yield and tillering of brachiarias sown at depths intercropped with corn

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Body and Didactics. Possible directions of international research

The paper investigates the role of the body in didactics. It looks up for points of contact between the functional sceneries of the classroom and some recent approaches, such like simplexity, neurosciences and enactivism. The two experiments presented they aim to demonstrate the importance of body awareness to improve the didactic quality. The first experience used a SenseWear Armband that provided data about the energetic expenditure of a teacher during diff erent activities in a lesson. Th e second experiment relied on a neurofeedback device integrated to a sensor, it detected body temperature with the aim of understanding the role of the body in the process of self-regulation-learning and management of attention and arousal.

High Levels of Diversity Uncovered in a Widespread Nominal Taxon: Continental Phylogeography of the Neotropical Tree Frog Dendropsophus minutus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)