Reconstrução do L.C.P. do joelho: técnica de fixação no leito tibial (INLAY). Avaliação objetiva e subjetiva de 30 casos

A reconstrução cirúrgica do ligamento cruzado posterior (L.C.P.) do joelho ainda permanece como um grande desafio terapêutico. Neste trabalho avaliamos 30 pacientes submetidos à reconstrução cirúrgica do L.C.P. com a técnica de fixação do enxerto tendíneo no leito tibial por abordagem direta (INLAY). 28 pacientes eram do sexo masculino e 2 do feminino, com idade média de 31,10 anos. O tempo médio de lesão foi de 34,24 meses em 67% dos casos a lesão foi secundária a acidente motociclístico. As lesões condrais e do ligamento cruzado anterior (L.C.A.) do joelho estavam presentes em 67% e 33% dos casos, respectivamente. Os pacientes foram avaliados objetivamente (teste de gaveta posterior) e subjetivamente (Escala de Lysholm). O seguimento pós-operatório médio foi de 21,7 meses. Cerca de 66% dos casos foram classificados como bom e excelente na avaliação subjetiva e objetiva. A análise estatística apresentou comportamento semelhante para as duas avaliações.Os resultados clínicos pós-operatórios obtidos neste trabalho têm nos encorajado a seguir com esta técnica cirúrgica.

Níveis de energia digestível para leitoas dos 30 aos 60 kg mantidas em ambiente frio (15°C)

Vinte e cinco leitoas dos 30 aos 60 kg PV, mantidas em temperatura ambiente baixa, foram usadas para avaliar o efeito de níveis de energia digestível de 3100 a 3700 kcal/kg de ração sobre desempenho, composição da carcaça e níveis séricos de hormônio da tireóide. O ganho de peso diário não foi influenciado pelo nível de energia digestível (ED) da ração, porém influiu de forma linear decrescente nos consumos de ração e proteína. A conversão alimentar melhorou de forma linear com o aumento do nível de ED da ração. O consumo de ED aumentou linearmente com a inclusão de óleo de soja na ração. Foi observado efeito do nível de ED da ração sobre a taxa de deposição de gordura e proteína, que aumentou e reduziu linearmente, respectivamente. As concentrações séricas de triiodotironina livre das leitoas aumentaram linearmente com o nível de energia da ração. O nível de energia da ração não influenciou os pesos absoluto e relativo dos órgãos avaliados.

Proteína bruta para suínos machos castrados mantidos em ambiente de conforto térmico dos 15 aos 30 kg

RESUMO - Este experimento foi realizado para determinar a exigência de proteína bruta para suínos mestiços (Landrace x Large White), machos, castrados dos 15 aos 30 kg, mantidos em ambiente de conforto térmico. Durante o período experimental, a temperatura da sala manteve-se em 23,1±1,19°C, com umidade relativa de 80,6±4,59% e índice de temperatura do globo e umidade de 69,85±1,38. Foi usado um total de 60 leitões mestiços, machos castrados, com peso médio inicial de 14,8±0,85kg e final de 29,3±2,42 kg. Foi usado delineamento de blocos ao acaso, com cinco tratamentos (17,0; 18,0; 19,0; 20,0; e 21,0% de proteína bruta), seis repetições e dois animais por unidade experimental. O nível de proteína bruta na ração influenciou o ganho de peso diário e os consumos de proteína e lisina diários, que aumentaram linearmente. Entretanto, a conversão alimentar diminuiu linearmente. Não houve efeito do nível de proteína sobre os consumos de ração e energia diários. A taxa de deposição de gordura não foi influenciada, enquanto a taxa de deposição de proteína aumentou quadraticamente até o nível de 20,0% de proteína bruta. Os pesos absolutos do fígado e do intestino e o peso relativo do fígado aumentaram linearmente com o crescente nível de proteína bruta da ração. A concentração de uréia plasmática não foi influenciada pelos níveis de proteína bruta da ração. Suínos mestiços, machos, castrados de 15 a 30 kg, mantidos em ambiente de conforto térmico exigem 20,0% de proteína bruta na ração, correspondente a 0,90% de lisina total ou 57 g de proteína/Mcal ED. O nível de uréia no plasma sangüíneo não foi um parâmetro adequado para estimar a exigência de proteína bruta.

SEX DETERMINATION IN BEES .30. EFFECTS OF JUVENILE-HORMONE ON THE DEVELOPMENT OF TERGAL GLANDS IN MELIPONA

Worker larvae of Melipona rufiventris, M. quadrifasciata and M. compressipes were treated topically with juvenile hormone (JH) during the cocoon-spinning phase. Examination of the tergites of the queens obtained following JH application showed induced differentiation into adults with full female (queen) traits. Melipona workers had tergal glands only in tergite II, whereas natural and JH-induced queens had tergal glands in tergite II plus additional glands in at least one other of tergites Ill to VII.

Deletion of Epstein-Barr virus latent membrane protein 1 gene in United States and Brazilian Hodgkin's disease and reactive lymphoid tissue: High frequency of a 30-bp deletion

A 30-basepair (bp) deletion in the Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1) gene has been reported in nasopharyngeal carcinoma and EBV-associated malignant lymphomas. Prior studies have found the deletion in about 10% to 28% of cases of Hodgkin's disease (HD), particularly in cases with aggressive histology. We studied the prevalence of 30-bp LMP1 gene deletion in EBV-positive HD in the United States (US) (12 cases) and Brazil (26 cases) with comparison to reactive lymphoid tissues (21 cases) and HD without EBV-positive Reed-Sternberg cells (15 cases). We studied the status of the LMP1 gene by Southern blot hybridization of polymerase chain reaction (PCR) products obtained after amplification with primers spanning the site of the deletion. We also performed EBV typing, EBER1 in situ hybridization, and LMP1 protein immunohistochemistry. EBV was detected in 12/26 (46%) cases of HD from the US and 26/27 (96%) cases of Brazilian HD. The 30-bp LMP1 gene deletion was observed in 4/12 (33%) cases of EBV-positive HD from US, and 12/26 (46%) cases of Brazilian EBV-positive HD, including 3 cases of type B EBV, as compared with 12/21 (57%) reactive lymphoid tissues and 9/15 (60%) cases of EBV-negative HD. US and Brazilian HD showed a higher prevalence of the 30-bp LMP1 gene deletion, compared with studies of others. The unexpected finding of high incidence of 30-bp deletion in LMP1 gene in reactive lymphoid tissue and HD without EBV-positive Reed-Sternberg cells suggests that this deletion may not be relevant to HD pathogenesis in most cases. Copyright (C) 1997 by W.B. Saunders Company.

Thermal analysis and electrochemical study of Hg reactions on Pt-30% Ir alloy electrode

Thermogravimetry (TG), cyclic voltammetry (CV) and other analytical techniques were used to study the reactions of mercury with Pt-30% Ir alloy. The results allowed to suggest that an electrodeposited mercury film interacts with the substrate and when subjected to heat or electrochemical removal at least four mass loss steps or five peaks appeared during the mercury desorption process. The first two steps were attributed to Hg(0) removal probably from the bulk and from the adsorbed monolayer which wets the electrode surface. These two processes are responsible for peaks D and F in the cyclic voltammograms. The last two peaks (G, H) in CV were ascribed to the intermetallic compound decomposition. In TG curves, the last two steps were attributed to the PtHg4 (third step), and PtHg2 decomposition followed by Hg removal from the subsurface. The PtHg2 was formed by an eutectoide reaction: PtHg -> PtHg2+Hg(Pt-Ir). The Hg diffused to the subsurface was not detectable by cyclic voltammetry.

Changes in coral reef communities and an associated reef fish species, Cephalopholis cruentata (Lacepede), after 30 years on Curacao (Netherlands Antilles)

Using the same methodology and identical sites, we repeat a study dating from 1973 and quantify cover of hard coral species, soft corals, sponges, hard substratum and soft substratum, and density of a commercially important reef fish species, the graysby Cephalopholis cruentata, along a depth-gradient of 3-36 m oil the coral reefs of Curacao. The objective was to determine the multi-decade change in benthic coral reef cover and structural complexity, and their effect oil densities of an associated reef fish species. Total hard coral cover decreased on average from 52% in 1973 to 22% in 2003, representing a relative decline of 58%. During this time span, the cover of hard substratum increased considerably (from 11 to 58%), as did that of soft corals (from 0.1 to 2.2%), whereas the cover of sponges showed no significant change. Relative decline of hard coral cover and of reef complexity was greatest in shallow waters (near the coast), which is indicative of a combination of anthropogenic influences from shore and recent storm damage. Cover of main reef builder coral species (Agaricia spp., Siderastrea siderea, Montastrea annularis) decreased more than that of other species, and resulted in a significant decrease in reef complexity. Although density of C. cruentata was highly correlated to cover of Montastrea and Agaricia in 1973, the loss of coral cover did not show any effect on the total density of C. cruentata in 2003. However, C. cruentata showed a clear shift in density distribution from shallow water in 1973 to deep water in 2003. It call be concluded that the reefs of Curacao have degraded considerably in the last three decades, but that this has had no major effect on the population size of one commercially important coral-associated fish species.

Estado, burguesía e legislação trabalhista brasileira no limiar dos anos 30: notas para uma discussão

The creation and implementation of Brazilian labor laws during the 1930s gave rise to important political debates. The various stakeholders - the state, the bourgeoisie, and workers - each with their own social project, had forged mechanisms designed to protect their own interests. Within this framework, this article addresses the arguments regarding the Expulsion of Foreigners Act (1907), the "Dois Tercos"(Two-thirds) Act (1930), and the Unionization Act (1931). These acts provide clear evidence that the growth of state interventionism was accompanied by tensions and changes, and that the labor legislation was not simply the 'gift' of a Corporate State.

O pensamento e o metodo na obra de Pierre Monbeig - analise dos trabalhos produzidos no Brasil nas decadas de 30 e 40.

Examines the historiography of the geographical ideas in Brazil starting from the analysis of Monbeig's work which has two essential elements: the positivist-functionalist discourse, and the ideals of the politic liberalism that are his main preoccupation. The preoccupation of geography as a science is constant in all his discourse. -English summary

Electrocardiographic evolution in cats from birth to 30 days of age

Further knowledge of feline physiology and neonatology is needed because the number of cats being kept as pets and used as experimental animals has increased. Few studies have been published on feline sequential electrocardiography. This study was conducted to determine the values of normal waves, complexes, and intervals of the electrocardiogram in clinically healthy feline neonates. Serial electrocardiography was performed in 15 female and 15 male neonates at 30 days of age. Parameters analyzed were heart rate and rhythm, duration and amplitude of electrocardiographic waves, duration of intervals, and the electrical axis of the heart. The results did not show differences between males and females. During the neonates' first 30 days of life, migration of the electrical axis from right to left was observed. There was a progressive increase in the R wave amplitude, while the S wave amplitude showed a progressive decrease. A sinus heart rhythm was predominant in the feline neonates.

Evaluating the karyotypic diversity in species of Hyla (Anura, Hylidae) with 2n = 30 chromosomes based on the analysis of ten species

Ten species of Hyla with 2n = 30 from Brazilian fauna were analysed cytogenetically. Hyla minuta is the unique presenting all bi-armed metacentric or submetacentric chromosomes in the karyotype, therefore, with the highest FN = 60. The remaining species have a variable number of uni-armed telocentric or subtelocentric chromosomes: H. cruzi, H. elianeae, and H. rubicundula with three pairs (FN = 54), H. berthalutzae, H. elegans, H. microps, and H. nana with four pairs (FN = 52), and H. nahdereri and H. sanborni with five pairs (FN = 50). The uni-armed elements are among pairs 5, 6, 7, 11, 14, and 15, which also appeared with metacentric or submetacentric morphology. The remaining chromosome pairs 1, 2, 3, 4, 8, 9, 10, 12, and 13 were never found to be telocentric or subtelocentric. AgNOR patterns are species-specific, the majority of the species exhibiting a single pair with AgNORs, with the exception of H. elegans and H. nana with more than one chromosome pair bearing this cytological marker. C banding was obtained in H. berthalutzae, H. cruzi, H. elegans, H. elianeae, H. microps, H. minuta, H. nahdereri, and H. nana, which showed positively stained centromeric heterochromatin. Our analysis confirms the great karyotypic diversity in the species of Hyla with 2n = 30, with no species sharing identical karyotypes.