Effects of LLLT in combination with bisphosphonate on bone healing in critical size defects: A histological and histometric study in rat calvaria

The purpose of this study was to analyze histologically the effect of low-level laser therapy (LLLT) in combination with bisphosphonate on bone healing in surgically created critical size defects (CSD) in rat calvaria. One hundred Wistar female rats sham operated (sham) and ovariectomized (Ovx) were maintained untreated for 1 month to allow for the development of osteopenia in the Ovx animals. A CSD was made in the calvarium of each rat, and the animals were divided into five groups according to following treatments: (1) sham rats (control), (2) Ovx rats, (3) Ovx rats treated with LLLT, (4) Ovx rats treated with bisphosphonate, and (5) Ovx rats treated with bisphosphonate and LLLT. Groups 4 and 5 were irrigated with 1 ml of bisphosphonate, and groups 3 and 5 were submitted to LLLT (GaAlAs), 660 nm, 24 J, and 0.4285 W/cm2 on the CSD. Ten animals of each treatment were killed at 30 and 60 days. Histomorphometric assessments, using image analysis software, and histological analyses were performed. No defect was completely regenerated with the bone. Histometrically, it can be observed that groups 3 (37.49 ± 1.94%, 43.11 ± 2.39%) and 5 (35.05 ± 1.57%, 41.07 ± 1.89%) showed a significant bone neoformation when compared to groups 1 (16.81 ± 1.57%, 27.54 ± 1.49%), 2 (11.68 ± 0.98%, 22.51 ± 1.05%), and 4 (14.62 ± 1.70%, 25.67 ± 1.41%) in all experimental periods (P < 0.05). It was possible to conclude that the LLLT associated or not with bisphosphonate treatment was effective for stimulating bone formation in CSD in the calvaria of rats submitted to ovariectomy. © 2012 Springer-Verlag London Ltd.

Bone marrow aspirate combined with low-level laser therapy: A new therapeutic approach to enhance bone healing

This study evaluated the influence of bone marrow aspirate (BMA), low-level laser therapy (LLLT) and their combination on bone healing in surgically created critical-size defects (CSDs) in rat calvaria. 40 rats were divided into four groups: C (control), BMA, LLLT and BMA/LLLT. A 5 mm diameter CSD was created in the calvarium of each animal. In Group C, the defect was filled by blood clot only. In Group BMA, the defect was filled with BMA. In groups LLLT and BMA/LLLT, the defect received laser irradiation (InGaAlP laser), was filled with blood clot or BMA respectively, and irradiated again. Animals were euthanized 30 days postoperatively. Histomorphometric and immunohistochemical analyses were performed. Newly formed bone area (NFBA) was calculated as percentage of the total area of the original defect. Proliferating cell nuclear antigen (PCNA), runt-related transcription factor 2 (Runx2) and osteocalcin (OCN) immunohistochemical staining were performed. PCNA-positive, Runx2-positive and OCN-positive cells were quantified. Data were statistically analyzed. Group BMA/LLLT had significantly greater NFBA than groups C, BMA or LLLT. Group BMA presented significantly greater NFBA than control, while group LLLT did not. Group BMA/LLLT presented a significantly higher number of PCNA-positive and OCN-positive cells than any of the other groups. Groups BMA/LLLT and BMA showed a significantly lower number of Runx2-positive cells than groups C or LLLT. The combination of BMA/LLLT yielded significantly greater bone formation in surgically created CSD in rat calvaria when compared to control, or either treatment alone. © 2013 Elsevier B.V. All rights reserved.

Cicatrização óssea de defeitos de tamanho crítico tratados com Aspirado de Medula Óssea, Laser em Baixa Intensidade ou a associação de ambas as terapias: estudo histológico e histométrico em calvárias de ratos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Reparo ósseo de defeitos de tamanho crítico tratados com plasma rico em plaquetas derivado do sangue periférico ou do aspirado de medula óssea associado ou não ao enxerto de osso autógeno: estudo histológico e histométrico em calvárias de ratos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Processo de cicatrização óssea em defeitos cirúrgicos de tamanho crítico tratados com partículas de vidro bioativo associadas ou não à barreira de sulfato de cálcio: estudo histológico e histométrico em calvárias de rato

Pós-graduação em Odontologia - FOA

Influência do plasma rico em plaquetas no reparo ósseo de defeitos de tamanho crítico em calvárias de ratos: estudo histológico e histométrico

Pós-graduação em Odontologia - FOA

Sentimentos e percepções de mulheres no ciclo gravídico-puerperal que sobreviveram à morbidade materna grave

Pós-graduação em Enfermagem (mestrado profissional) - FMB

Atendimento de urgência/emergência pré-hospitalar a indivíduos com autonomia reduzida vitimados por atos de violência: dificuldades e dilemas éticos

Pós-graduação em Enfermagem (mestrado profissional) - FMB

Formação profissional e inserção no mercado de trabalho: percepções de um curso de graduação em enfermagem de instituição privada

Pós-graduação em Enfermagem (mestrado profissional) - FMB

Compreendendo a experiência da equipe multiprofissional em uma unidade de queimados

Pós-graduação em Enfermagem (mestrado profissional) - FMB

Processo de cicatrização óssea em defeitos cirúrgicos de tamanho crítico tratados com partículas do novo vidro bioativo associadas ou não à barreira de sulfato de cálcio: estudo histológico e histométrico em calvárias de rato

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do reparo ósseo de defeitos de tamanho crítico tratados com plasma rico em plaquetas ou com fibrina rica em plaquetas: estudo histomorfométrico e imunoistoquímico em calvárias de ratos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência da associação do plasma rico em plaquetas ao enxerto de osso autógeno no reparo ósseo de defeitos de tamanho crítico em calvárias de coelhos: estudo histológico e histométrico

Pós-graduação em Odontologia - FOA

Compreendendo a experiência de crianças e adolescentes submetidos à punção de medula óssea e lombar em centro cirúrgico de um hospital oncológico

Pós-graduação em Enfermagem (mestrado profissional) - FMB

Representações de indivíduoas em situações de isolamento para transplante de medula óssea

Pós-graduação em Enfermagem (mestrado profissional) - FMB