Análise estrutural dos efeitos da desadaptação em componentes de próteses odontológicas utilizando o método dos elementos finitos com elemento de contato e pré-carga

Pós-graduação em Engenharia Mecânica - FEB

Avaliação funcional e histológica do enxerto tubular de matriz acelular arterial heteróloga como substituto da uretra em coelhos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Understanding the Function of Conserved Variations in the Catalytic Loops of Fungal Glycoside Hydrolase Family 12

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Kinome profiling of osteoblasts on hydroxyapatite opens new avenues on biomaterial cell signaling

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Bioengenharia no controle de processos erosivos de áreas de hidrelétricas – o caso da PCH Costa Rica (MS)

The crescent increasing demand of the electric power in Brazil has stimulated the implantation of Small Central Hydroelectric Power (SCHP) in several regions of the country. However, the silting up of the reservoirs is one of the main problems faced by hydroelectric power plants and SCHP. In this context, this research aimed evaluate the phenomenology and propose the enforcement of appropriate bioengineering techniques to control the intense erosive process of the hydrographical basin of the “Alto Rio Sucuriú,” that cause silting up of the reservoirs of the SCHP Costa Rica, located in the municipal district of Costa Rica (MS). In order to identify the phenomenology of the main falling in of this basin, a diagnosis of the conditions of the physical environment of the region (climate, geology, pedology, hydrology, and use of the ground) was realized. A surveying was also realized to specify the geometric feature of the falling in using the Total Station of the Ruide brand, series RTS 860R and the geodetic GPS of the Ashtech brand and the data obtained was used on the preparation of the Digital Elevation Model (DEM) of the falling in. Based on this diagnosis an official register of the falling in was done with identification of the different types of present erosion. Due to the advanced erosive stage of the falling in researched, the use of bioengineering techniques could be the best solution considering that the traditional engineering techniques make use of heavy material like concrete, iron and large machines that besides causing higher impact to the natural and esthetic aspects of the environment also require a higher investment of capital. This research establish a great cooperation to the knowledge of the erosive process and of the rehabilitation of the degraded areas with application of bioengineering techniques not only hydrographical basin of “Alto Rio Sucuriú” but also to other basins that show similar situation

Técnicas de Bioengenharia Aplicadas ás Áreas de Preservação Permanente Urbanas – Presidente Prudente, São Paulo

There is a demand to incorporate the nature in the city in order to meet the wishes of the entire population. The valuation of water, a key component of the quality of the landscape, becomes a key element in this context both for its ecological potential because of their potential for recreational activities. The areas that suffer most impacts are the Permanent Preservation Areas (PPAs) and this paper will be a weighting on the urban interface - PPA. With the study area the basin of the stream of Limoeiro, in the context of the Friendship Fountain Balneary, especially the source contained between residential neighborhoods Maré Mansa and Parque Imperial, the city of Presidente Prudente and Álvares Machado, Sao Paulo, this paper aims to search and use of knowledge about technologies and techniques of environmental restoration, with emphasis on bioengineering, through an environmental characterization of the basin, with a view the incorporation of nature in the lives of the urban population. The methodology includes: literature review priority issues related to the PPAs, linear parks, relevant environmental legislation, environmental characterization of the watershed of the stream Limoeiro; study of technologies and techniques for environmental remediation of soil bioengineering and implementation of parks linear, development of proposals for the environmental recovery of PPA in the basin of the stream Limoeiro, focusing on emerging... (Complete abstract click electronic access below)

Expansão ex vivo de células uroteliais em scaffolds 3D

Malformations and possible damages to the urogenital system can be originated in the embryonic period. Moreover, fire guns, knives and accidents, where there is the disruption of the urethra, also cause these lesions. The objective was to analyze the contribution of tissue engineering in the construction of neo-urethra, developed by bioengineering. We performed an urothelial ex vivo expansion of cells in 3D scaffolds (platelet gel matrix and acellular porcine aorta) to assess the contribution of this technique in the construction of a neo-urethra. Mechanical dissociation was made of the inner wall of 10 North Folk rabbit’s bladder, weighing 2.5 to 3.0 kg. After dissociation the cell content was centrifuged and obtained a pellet of urothelial cells. The pellet was ressuspended in culture medium DMEM F12 and cells were maintained in culture for 15 days. Immunohistochemical analysis characterized the urothelial culture. The cells were then implanted in the scaffold - platelet gel. In a second experiment using aortic porcine acellular matrix were implanted urothelial cells alone and urothelial cells on platelet gel, on the inner wall of the scaffold - aorta, with space for setting bordered by a urethral probe. The complex probe - cells - aorta and probe - cells in platelet gel - aorta, were sealed with suture material and culture were maintained in a humidified 37ºC incubator with 5% CO2 in air for 12 days to subsequent histological analysis of urothelium cell adhesion to the scaffolds. By observation under an optical microscope, we could see the growth of cells in the scaffold platelet gel, from a monolayer in to a three-dimensional structure. In the acellular porcine aortic matrix containing the platelet gel, we could observe a few quantity of urothelial cells adhered. However with the acellular porcine aortic matrix in which was implanted only the urothelial cells, we have obtained adhesion to the wall

Scientific use of the finite element method in Orthodontics

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Optimal control strategy for fed-batch enzymatic hydrolysis of lignocellulosic biomass based on epidemic modeling

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration

Bacterial cellulose (BC) and silk fibroin (SF) are natural biopolymers successfully applied in tissue engineering and biomedical fields. In this work nanocomposites based on BC and SF were prepared and characterized by scanning electron microscopy (SEM), infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). In addition, the investigation of cytocompatibility was done by MTT, XTT and Trypan Blue dye technique. Cellular adhesion and proliferation were detected additionally. The evaluation of genotoxicity was realized by micronucleus assay. In vitro tests showed that the material is non-cytotoxic or genotoxic. SEM images revealed a greater number of cells attached at the BC/SF:50% scaffold surface than the pure BC one, suggesting that the presence of fibroin improved cell attachment. This could be related to the SF amino acid sequence that acts as cell receptors facilitating cell adhesion and growth. Consequently, BC/SF:50% scaffolds configured an excellent option in bioengineering depicting its potential for tissue regeneration and cultivation of cells on nanocomposites.