Action of anti-bothropic factor isolated from Didelphis marsupialis on renal effects of Bothrops erythromelas venom

Acute renal failure is the most common complication in the lethal cases caused by snakebites in Brazil. Among the Brazilian venom snakes, Bothrops erythromelas is responsible for the majority of accidents in Northeastern Brazil. Didelphis marsupialis serum could inhibit myonecrotic, hemorrhagic, edematogenic hyperalgesic and lethal effects of envenomation determined by ophidian bites. In the present study, we evaluated the action of the anti-bothropic factor isolated from D. marsupialis on the renal effects promoted by B. erythromelas venom without systemic interference. Isolated kidneys from Wistar rats were perfused with Krebs-Henseleit solution containing 6% bovine serum albumin. We analyzed renal perfusion pressure (PP), renal vascular resistance (RVR), glomerular filtration rate (GFR), urinary flow (UF), and the percentages of sodium and potassium tubular transport (%TNa +, %TK +). The B. erythromelas venom (10 μg mL -1) decreased the PP (ct=108.71±5.09 mmHg; BE=65.21±5.6 mmHg*) and RVR (ct=5.76±0.65 mmHg mL -1 g -1 min -1; BE=3.10±0.45 mmHg mL -1 g -1 min -1*) . On the other hand, the GFR decreased at 60 min (ct 60=0.76±0. 07 mL g -1 min -1; BE 60=0.42±0.12 mL g -1 min -1*) and increased at 120 min (ct 120=0.72±0.01 mL g -1 min -1; BE 120=1.24±0.26 mL g -1 min -1*). The UF increased significantly when compared with the control group (ct=0.14±0.01 mL g -1 min -1; BE=0.47±0.08 mL g -1 min -1*). The venom reduced the %TNa + (ct 90=79.18±0.88%; BE 90=58.35±4.86%*) and %TK + (ct 90=67.20±4.04%; BE 90=57. 32±5.26%*) The anti-bothropic factor from D. marsupialis (10 μg mL -1) incubated with B. erythromelas venom (10 μg mL -1) blocked the effects on PP, RVR, %TNa +, and %TK +, but was not able to reverse the effects in UF and GFR promoted by venom alone. However, the highest concentration of D. marsupialis serum (30 μg mL -1) reversed all the renal effects induced by the venom. In conclusion, B. erythromelas venom altered all the renal functional parameters evaluated and the anti-bothropic factor from D. marsupialis was able to inhibit the effects induced by the venom in isolated kidney. © 2005 Elsevier Ltd. All rights reserved.

Effects of electroplated zinc-nickel alloy coatings on the fatigue strength of AISI 4340 high strength steel

It is well known that fatigue behaviour is an important parameter to be considered in mechanical components subjected to constant and variable amplitude loadings. In combination with corrosion phenomenon, fatigue effects were responsible for proximally 64% of fails that occur in metallic parts of aeronautical accidents in the last 30 years. Recovered substrates have been extensively used in the aerospace field. Cadmium electroplating has been widely applied to promote protective coatings in aeronautical components, resulting in excellent corrosion protection combined with a good performance in cyclic loading. Ecological considerations allied to the increasing demands for corrosion resistance, resulted in the search for possible alternatives. Zinc-nickel alloys received considerable interest recently, since these coatings showed some advantages such as a good resistance to white and red rust, high plating rates and acceptation in the market. In this study the effects of zinc-nickel coatings electroplated on AISI 4340 high strength steel were analysed on rotating bending and axial fatigue strength, corrosion and adhesion resistance. Compressive residual stress field was measured by a X-ray tensometry prior to fatigue tests. Optical microscopy images showed coating thicknesses, adhesion and the existence of an uniform coverage of nearly all substrates. The fractured fatigue specimens were investigated using a scanning electron microscope. Three different zinc-nickel coating thicknesses were tested and comparison with rotating bending fatigue data from specimens cadmium electroplated and heat treated at 190°C for 3, 8 and 24 hours to avoid the diffusion of hydrogen in the substrate, was performed. Experimental results showed effect of coatings on the AISI 4340 steel behaviour when submitted to fatigue testing and the existence of coating thickness influence on the fatigue strength.

Exposição ocupacional ao ruído e acidentes do trabalho

The purpose of this study was to verify whether occupational noise exposure is a significant risk factor for work accidents in the city of Piracicaba, São Paulo State, Brazil. This hospital-based case-control study included 600 workers aged 15-60 who suffered typical occupational accidents between May and October 2004 and were seen at the Piracicaba Orthopedics and Trauma Center. The control group comprised 822 workers, aged 15-60, who were also seen at the Center, and either had a non-occupational accident or were accompanying someone who had suffered an accident. A multiple logistic regression model was adjusted with work accident as an independent variable, controlled by covariables of interest such as noise exposure. The risk of having a work accident was about twice as high among workers exposed to noise, after controlling for several covariables. Occupational noise exposure not only affected auditory health status but was also a risk factor for work accidents.

Spatial clustering applied to health area

The significant volume of work accidents in the cities causes an expressive loss to society. The development of Spatial Data Mining technologies presents a new perspective for the extraction of knowledge from the correlation between conventional and spatial attributes. One of the most important techniques of the Spatial Data Mining is the Spatial Clustering, which clusters similar spatial objects to find a distribution of patterns, taking into account the geographical position of the objects. Applying this technique to the health area, will provide information that can contribute towards the planning of more adequate strategies for the prevention of work accidents. The original contribution of this work is to present an application of tools developed for Spatial Clustering which supply a set of graphic resources that have helped to discover knowledge and support for management in the work accidents area. © 2011 IEEE.

Ação educativa de prevenção de acidentes domésticos em escolas de ensino fundamental

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Mapeamento de sensibilidade a derramamentos de petróleo do Parque Estadual da Ilha do Cardoso - PEIC e áreas do entorno

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise de acidentes fatais investigados pelo MTE de acordo com o porte da empresa: Estado de Sâo Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Implantação e análise de sistema de captação de acidentes do trabalho no Hospital Escola da Faculdade de Medicina de Botucatu

Pós-graduação em Saúde Coletiva - FMB

Percepção dos riscos de escorregamentos na Vila Mello Reis, Juiz de Fora (MG): contribuição ao planejamento e à gestão urbanos

Pós-graduação em Geografia - IGCE

Análise dos riscos físicos e ergonômicos em roçadora transversal motorizada

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Biologia, morfologia e bioquímica de veneno da formiga lava-pés Solenopsis saevissima Smith (Insecta, Hymenoptera, Formicidae)

Pós-graduação em Ciências Biológicas (Zoologia) - IBRC

Acidentes graves fatais no trabalho de corte mecanizado de cana-de-açucar: o olhar através do método mapa

Pós-graduação em Saúde Coletiva - FMB

Caracterização gfeológico-geotécnica aplicada a gestão de risco no gasoduto GASBOL, trecho Ipeúna/SP - Itirapina/SP

The pipelines are characterized by the great length and linearity, these enterprises are usually associated with the horizon of soil and decomposed rock and accidents in this system usually have high complexity. So this work proposes a study of geological and geotechnical characterization of approximately 40 km ² and a study of risk analysis along 18.3 km of gas pipeline GASBOL, in the municipalities of Ipeúna-SP and Itirapina-SP, in scale 1:25.000, besides correlation between studies. The method for geological and geotechnical characterization is based on physiographic integrated analysis technique, which is supported by literature research, observation and interpretation of satellite imagery (photointerpretation), correlation of products numerical model of ground, fieldwork. The method adopted for risk classification is based on preliminary analysis of hazards (APP) technique, which is identified the geological process, this feature is classified as the susceptibility of occurrence, and then classified as the possible consequence of the process, if this evolves. As a product of the research, we designed a map in 1:25,000 scale, containing a table of geological and geotechnical characteristics of the mapped drives, as well as sectorization risk in each section of the pipeline. Were mapped geological and geotechnical seven units in the study area, delimited in excerpts of which five are classified as high risk, totaling 1.1 km in length of 18.3 km total

Estudos estruturais por difração de raios X de fosfolipases A2 em presença de íons com importância funcional

Snakes from Bothrops genus are responsible for more than 90% of the ophidian accidents in Brazil. One of the main complications from this kind of accident is muscular necrosis, which is related to the action of phospholipases A2 and metalloproteases, two groups of enzymes found in the venom of these animals. Although this complication cannot be solved by serum therapy administration, a great number of studies have been performed with the attempt to know the pharmacological sites of these toxins aiming, in the future, the development of complementary treatments to serum therapy. This work proposes structural studies of bothropic phospholipases A2 (PLA2s) in the presence of ions relevant to their activity, using the X-ray crystallography technique. Recently, it was demonstrated ions, as manganese, calcium and others, interfere in the biological activity of the PLA2s. Particularly, Lys49-PLA2s in the presence of manganese ions have miotoxicity reduced. Asp49-PLA2s show catalytic activity dependent of calcium, although structural studies with a miotoxic Asp49-PLA2, BthTX-II, suggest a possible catalytic mechanism independent of calcium. Therefore, co-crystallization of BthTX-II in the presence of calcium ions and of PrTX-I in the presence of manganese ions were performed. Comparative structural studies among obtained results and others already published in the literature were performed aiming a better understanding of the structure-function relationship of these toxins. The BthTX-II with the presence of calcium do not show this ion in the loop of coordination of calcium, presence necessary to develop the catalyses. After comparison of this model with the native one, only one distortion was found, but no apparent relationship with the residues responsible for its activity. In the PrTX-I structure, regions candidates of manganese ions were also found... (Complete abstract click electronic access below)

Projeto de um transdutor binocular utilizando extensometria e aplicação na pesagem rodoviária

With the passage of time, the variety of companies from each branch has gradually grown throughout the country. As a consequence, the logistics to attend to such demand has grown in the same proportion. One factor to highlight about this rapid growth is the increase of freight vehicles roaming the roads, often carrying loads over the limit established by law. At first it may seem that this would not bring any problem, however, note that an excessive number of overloads ends up deteriorating the asphalt, causing ondulations, potholes and other imperfections on the roads, which can cause accidents. In addition, the money spent for the maintenance of the roads is high. Thinking about it, it was established by law a regulation that restricts the weight limit for these vehicles, through axle weighing, subject to a fine. With this control the road floor is preserved for longer, requiring less maintenance expenses. The weighing stations use load cells to display the inferred weight, these cells use strain gages in its construction. The following work presents a methodology to dimension a binocular load cell, its main elements and its dimensioning. Showing how different materials can affect the dimensioning, so that can work satisfactorily. It also presents an overview of how are the parameters for weighing, and fines