Anestesia epidural com ropivacaína, lidocaína ou associação de lidocaína e xilazina em cães: efeitos cardiorrespiratório e analgésico

Este estudo investigou o efeito da ropivacaína, da lidocaína e da associação de lidocaína e xilazina na anestesia epidural de cães. Trinta cães foram tranqüilizados com acepromazina intravenosa, distribuídos em três grupos e submetidos à anestesia epidural no espaço lombo-sacro, com lidocaína 2% com vasoconstrictor (GL), ropivacaína 1% (GR) ou com xilazina associada à lidocaína (GXL). Mensuraram-se as freqüências cardíaca (FC) e respiratória (f), a pressão arterial sistólica (PAS), a concentração final expirada de CO2 (EtCO2), o volume minuto (VM) e a temperatura retal (T). Para avaliação da analgesia somática, utilizou-se o teste do panículo e o teste térmico a 55°C. Os protocolos produziram anestesia da região retro-umbilical, sendo que a associação XL produziu bloqueio anestésico mais cranial, porém causou bradicardia moderada. A duração da anestesia foi mais prolongada nos animais dos grupos GXL (240 min) e GR (250 min), quando comparada as do grupo GL (120 min).

Comparison among three approaches for evaluation of sexual precocity in Nellore cattle

Restricted breeding seasons used in beef cattle produce censored data for reproduction traits measured in regard to these seasons. To analyze these data, adequate methods must be used. The objective of this paper was to compare three approaches aiming to evaluate sexual precocity in Nellore cattle. The final data set contained 6699 records of age at first conception (AFC14) (in days) and of heifer pregnancy (HP14) (binary) obtained from females exposed to the bulls for the first time at about 14 months of age. Records of females that did not calve in the following year after being exposed to a sire were considered censored (77.5% of total). The models used to obtain genetic parameters and expected progeny differences (EPDs) were a Weibull mixed and a censored linear model for AFC14 and threshold model for HP14. The mean heritabilities obtained were 0.76 and 0.44, respectively, for survival and censored linear models (for AFC14), and 0.58 for HP14. Ranking and Pearson correlations varied (in absolute values) from 0.54 to 0.99 (considering different percentages of sires selected), indicating moderate changes in the classification. Considering survival analysis as the best selection criterion (that would result in the best response to selection), it was observed that selection for HP14 would lead to a more significant decrease in selection response if compared with selection for AFC14 analysed by censored linear model, from which results were very similar to the survival analysis.

Orbital solitary fibrous tumor: Report of two cases and literature review

Background: Solitary fibrous tumor (SFT) is a rare, benign, and very uncommon lesion in the orbit. Because of its complex and variable clinical and histological appearance the SIFT is often misdiagnosed.Cases: Two new cases of orbital SIFT are reported, one in a man and the other in a woman, both unilateral and in the superomedial orbit.Observations: Clinical and tomographical evaluations were conducted and the lesions were excised. The histological evaluation showed the tumors were composed of spindle-shaped cells within colla.-en bundles and vascular channels. Immunohistochemical staining was positive for CD 34 and negative for S-100 protein.Conclusion: Immunohistochemical study is an important adjuvant in determining the SIFT diagnosis. Long-term follow-up is necessary because of the possibility of SFT recurrence after excision. (C) 2003 Japanese Ophthalmological Society.

Evaluation of ten tropical grasses in the northwest region of the state of Sao-Paulo Brazil

The experiment was carried out in the northwest region of the State of São Paulo-Brazil to evaluate ten grasses recently introduced in the region. The grasses studied were: Cynodon nlemfuensis cv. Tifton 68, C dacrylon cv. Tifton 78, Cynodon spp. cv. Tifton 85, C. dactylon cv. Florakirk, C. nlemfuensis cv. Florico, C nlemfuensis cv. Florona, C dactylon cv. Coastcross, Paspalum notation cv. Tifton and NDF (717 to 749 g.kg(-1)) were observed in the Cynodon cultivars while lower values of CP (102 and 107 g.kg(-1)) and NDF (697 and 705 g.kg(-1)) were registered in the cvs. Marandu and Tanzania 1. The contents of ADF were higher in the the cvs. Tanzania 1 and Tifton 9. The average values of IVDMD differed (Ppound0.05) among grasses and were all above 564 g.kg(-1), except for cv. Tifton-9 (499 g.kg(-1)). The results obtained in this work allowed to conclude that the cvs. Tifton 78, Tifton 85, Coastcross, Florona, and Tanzania 1 are interesting options for pastures establishment in the northwest region of the State of São Paulo-Brazil.

Evaluation of tissue reaction to Aroeira (Myracrodruon urundeuva) extracts: a histologic and edemogenic study

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Higher salt preference in heart failure patients

Heart failure (HF) is a complex syndrome that involves changes in behavioral, neural and endocrine regulatory systems. Dietary salt restriction along with pharmacotherapy is considered an essential component in the effective management of symptomatic HF patients. However, it is well recognized that HF patients typically have great difficulty in restricting sodium intake. We hypothesized that under HF altered activity in systems that normally function to regulate body fluid and cardiovascular homeostasis could produce an increased preference for the taste of salt. Therefore, this study was conducted to evaluate the perceived palatability (defined as salt preference) of food with different concentrations of added salt in compensated chronically medicated HF patients and comparable control subjects. Healthy volunteers (n = 25) and medicated, clinically stable HF patients (n = 38, NYHA functional class II or III) were interviewed and given an evaluation to assess their preferences for different amounts of saltiness. Three salt concentrations (0.58, 0.82, and 1.16 g/100 g) of bean soup were presented to the subjects. Salt preference for each concentration was quantified using an adjective scale (unpleasant, fair or delicious). Healthy volunteers preferred the soup with medium salt concentration (p = 0.042), HF patients disliked the low concentration (p < 0.001) and preferred the high concentration of salted bean soup (p < 0.001). When compared to healthy volunteers, HF patients demonstrated a significantly greater preference for the soup with a high salt concentration (p = 0.038). It is concluded that medicated, compensated patients under chronic treatment for HF have an increased preference for salt. (C) 2011 Elsevier Ltd. All rights reserved,

The Mechanism of Oocyte Activation Influences the Cell Cycle-Related Genes Expression During Bovine Preimplantation Development

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Production of pro- and anti-inflammatory cytokines by monocytes from patients with paracoccidioidomycosis

Monocytes and macrophages can produce a large repertoire of cytokines and participate in the pathogenesis of granulomatous diseases. We investigated the production of pro- and anti-inflammatory cytokines by monocytes from patients with active paracoccidioidomycosis. Peripheral blood monocytes from 37 patients and 29 healthy controls were cultivated with or without 10 mug/ml of lipopolysaccharide (LPS) for 18 h at 37 degreesC, and the cytokine levels were determined in the culture supernatants by enzyme immunoassay. The results showed that the endogenous levels of tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1beta), IL-6, IL-8, IL-10 and transforming growth factor beta detected in the supernatant of patient monocytes cultivated without stimulus were significantly higher than those produced by healthy controls. These data demonstrated that monocytes from patients with active paracoccidioidomycosis produce high levels of cytokines with both inflammatory and anti-inflammatory activities. However, patient monocytes produced significantly lower TNF-alpha and IL-6 levels in response to LPS when compared to normal subjects, suggesting an impairment in their capacity to produce these cytokines after LPS stimulation. Concentrations of IL-1beta, IL-8 and IL-10 in cultures stimulated with LPS were higher in patients than in controls. These results suggest that an imbalance in the production of pro- and anti-inflammatory cytokines might be associated with the pathogenesis of paracoccidioidomycosis. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.

Physical chromosome mapping of repetitive DNA sequences in Nile tilapia Oreochromis niloticus: Evidences for a differential distribution of repetitive elements in the sex chromosomes

Repetitive DNAs have been extensively applied as physical chromosome markers on comparative studies, identification of chromosome rearrangements and sex chromosomes, chromosome evolution analysis, and applied genetics. Here we report the characterization of repetitive DNA sequences from the Nile tilapia (Oreochromis niloticus) genome by construction and screening of plasmid library enriched with repetitive DNAs, analysis of a BAC-based physical map, and hybridization to chromosomes. The physical mapping of BACs enriched with repetitive sequences and C(o)t-1 DNA (DNA enriched for highly and moderately repetitive DNA sequences) to chromosomes using FISH showed a predominant distribution of repetitive elements in the centromeric and telomeric regions and along the entire length of the largest chromosome pair (X and Y sex chromosomes) of the species. The distribution of repetitive DNAs differed significantly between the p arm of X and Y chromosomes. These findings suggest that repetitive DNAs have had an important role in the differentiation of sex chromosomes. (c) 2007 Elsevier Ltd. All rights reserved.

Gastrointestinal parasites in goats from Monte Castelo, Santa Catarina, Brazil

Este trabalho foi realizado com o objetivo de estimar o grau de infecção dos helmintos gastrintestinais em um rebanho caprino criado no Planalto Norte Catarinense. Foram utilizadas 12 fêmeas jovens e 11 adultas, das quais, a cada 28 dias, foram coletadas amostras de fezes diretamente do reto, totalizando 12 coletas, para quantificação de ovos por grama de fezes (OPG) e cultivo de larvas através de pool das amostras positivas do mesmo grupo. A contagem de OPG variou de zero a 10.400 nos animais jovens e de zero a 7.600 nos adultos. As médias do OPG entre as coletas foram de 583,3 a 4.441,7 no grupo jovem e de 418,2 a 2.181,8 nos adultos, sendo observados ovos da ordem Strongylida, dos gêneros Moniezia e Toxocara, bem como oocistos de coccídeos. Os animais mais jovens foram os mais acometidos, sendo o gênero Haemonchus o mais prevalente.

Occurrence of Pseudosida ramosa Daday, 1904 (Crustacea: Cladocera: Ctenopoda) in marginal lakes of the Paranapanema River, with comments on its distribution.

Aim: Contribute to the understanding of the distribution of a littoral cladoceran, Pseudosida ramosa Daday, 1904, in Brazil, with special reference for the Paranapanema River basin (Southeast Brazil); Methods: The specimens were sampled in two floodplain lakes located in the middle Paranapanema River basin, in the boundary of São Paulo and Parana States, using a standard plankton net (50 [mu]m mesh size). Trinocular microscopic with camera lucida and milimetric scale was used for the observation and measurements of the specimens; Results: Only three individuals were found (in winter and summer), despite of an extensive sampling program during 17 consecutive months of observations in both lakes. The largest body length was 1,146 [mu]m. The taxonomical identification followed Korovchinsky (1992); Conclusions: This species have a wide geographical distribution in Brazil, but it is restricted to zones colonized by aquatic macrophytes and seems to occur in low population abundance. The scarcity of registers of P. ramosa demonstrates the necessity of deeper investigations of littoral habits as well as the important role of this kind of environment for the conservation of the rivers/reservoirs biota, including some possible rare species.

Subterranean termites (Isoptera: Rhinotermitidae): Exploitation of equivalent food resources with different forms of placement

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Toxicity effect of the acaricide fipronil in semi-engorged females of the tick Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae): Preliminary determination of the minimum lethal concentration and LC50

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

When Basking Is Not an Option: Thermoregulation of a Viperid Snake Endemic to a Small Island in the South Atlantic of Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A NEW and THREATENED INSULAR SPECIES of LANCEHEAD FROM SOUTHEASTERN BRAZIL

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)