Cytotoxic action of Brazilian propolis in vitro on canine osteosarcoma cells

Osteosarcoma (OSA) is a primary bone neoplasm frequently diagnosed in dogs. The biology of OSA in pet dogs is identical to that of pediatric patients, and it has been considered an excellent model in vivo to study human OSA. Since the individual response to chemotherapy is unpredictable and considering that propolis is a natural product with several biological properties, this work evaluated the cytotoxic action of propolis on canine OSA cells. The primary cell culture of canine OSA was obtained from the tumor of a dog with OSA. Cell viability was assessed after incubation with propolis, 70% ethanol (propolis solvent), and carboplatin after 6, 24, 48, and 72 h. Cell viability was analyzed by the crystal violet method. Data showed that canine OSA cells were sensitive to propolis in a dose- and time-dependent manner and had a distinct morphology compared to control. Its solvent (70% ethanol) had no effect on cell viability, suggesting that the cytotoxic action was exclusively due to propolis. Our propolis sample exerted a cytotoxic effect on canine OSA cells, and its introduction as a possible therapeutic agent in vivo could be investigated, providing a new contribution to OSA treatment. Copyright © 2012 John Wiley & Sons, Ltd.

Bioecologia do percevejo bronzeado Thaumastocoris peregrinus carpintero & dellapé (hemiptera: thaumastocoridae) em eucalipto e prospecção de inimigos naturais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Resistência de genótipos de eucalipto ao psilídeo-de-concha Glycaspis brimblecombei Moore (Hemiptera: psyllidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Manejo hídrico na produção de brotos e mudas de quatro clones de Eucalyptus spp

Pós-graduação em Ciência Florestal - FCA

Mito e concretude: a morte na poesia de Angelus Silesius

This study intends to get a general overview on the German Angelus Silesius (1624- 1677)’s poetry about the way he discusses death. For the purpose of postulating some hypotheses about the meaning of death in the historical period in which the poet lived, we will comment some poems by identifying clues which reclaim the period’s moral.

Ciência e tecnologia na literatura italiana de Dante a Leopardi

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)